https://github.com/junlabucsd/mother-machine-data
Tip revision: 4c1c689192c51e2a523d2b768863b56c46bc802f authored by rthiermann on 04 January 2024, 23:27:32 UTC
Merge branch 'main' of https://github.com/junlabucsd/mother-machine-data into main
Merge branch 'main' of https://github.com/junlabucsd/mother-machine-data into main
Tip revision: 4c1c689
readme.md
### Raw data location
https://drive.google.com/file/d/1xL8VQzO7oFj_I-HoYDbsN5yiaS4D5X_5/view?usp=share_link
### Imaging conditions
`Imaging interval` 150 seconds
`Resolution` 0.065 uM / pixel
Images were obtained on an inverted microscope (Nikon Ti-E) with Perfect Focus 3 (PFS3), 100x oil immersion objective (PH3, numerical aperture = 1.45), and Obis laser 488LX(Coherent Inc., CA) as a fluorescence light source, and an Andor NEO sCMOS (Andor Technology) camera. The laser power was 18 mW. The exposure time was 200 ms for phase contrast imaging and 50 ms for fluorescence.
### Experimental conditions
`Strain` _E. coli_ MG1655 F- λ- rph-1 DnaN-Ypet (kanamycin resistant)
`Growth medium` MOPS 0.4% glycerol + 11 amino acids. See reference for detailed media recipe.
`Temperature` 37C
### Reference
Fangwei Si, Guillaume Le Treut, John T. Sauls, Stephen Vadia, Petra Anne Levin, Suckjoon Jun,
Mechanistic Origin of Cell-Size Control and Homeostasis in Bacteria,
Current Biology,
Volume 29, Issue 11,
2019,
Pages 1760-1770.e7,
ISSN 0960-9822,
https://doi.org/10.1016/j.cub.2019.04.062.
(https://www.sciencedirect.com/science/article/pii/S0960982219304919)
