https://github.com/darylgohl/TaG-EM
Tip revision: 5dc9aa94c42e759c219de322ae067e817a5edf4c authored by darylgohl on 20 February 2025, 18:24:16 UTC
Add files via upload
Add files via upload
Tip revision: 5dc9aa9
TaG-EM_plasmid_map.gb
LOCUS TaG-EM_plasmid_map 8917 bp ds-DNA circular SYN 20-FEB-2025
DEFINITION synthetic circular DNA.
ACCESSION
VERSION .
KEYWORDS pJFRC12-10XUAS-IVS-myr::GFP.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct.
REFERENCE 1 (bases 1 to 8882)
AUTHORS Pfeiffer BD, Ngo TT, Hibbard KL, Murphy C, Jenett A, Truman JW and
Rubin GM
TITLE Refinement of Tools for Targeted Gene Expression in Drosophila.
JOURNAL Genetics. 2010 Aug 9. ():.
PUBMED 20697123
REFERENCE 2 (bases 1 to 8882)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
COMMENT ##Imported_From-START##
Path :: /Users/dmgohl/Desktop/Projects/UMGC_IL_Project_038_TAG-
EM
Filename :: addgene-plasmid-26222-sequence-12326.gbk
##Imported_From-END##
FEATURES Location/Qualifiers
source 1..8917
/organism="synthetic DNA construct"
/mol_type="other DNA"
regulatory 31..135
/note="Geneious type: promoter"
/gene="bla"
/standard_name="AmpR promoter"
CDS 136..996
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="confers resistance to ampicillin, carbenicillin,
and related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
/standard_name="AmpR"
primer_bind complement(354..373)
/note="Ampicillin resistance gene, reverse primer"
/standard_name="Amp-R"
rep_origin 1167..1755
primer_bind 1656..1675
/note="pBR322 origin, forward primer"
/standard_name="pBR322ori-F"
protein_bind 1842..1911
/bound_moiety="phage phi-C31 integrase"
/note="attB site for the phi-C31 integrase (Groth et al.,
2000)"
/standard_name="attB"
primer_bind complement(2136..2154)
/note="Drosophila mini-white gene, reverse primer"
/standard_name="pCasper-F"
CDS join(2606..2677,3079..3352,3427..4081,4143..4458,
4662..4793,4864..5478)
/codon_start=1
/gene="white"
/product="Drosophila white gene eye color pigment"
/note="This is a modified version of the white gene
lacking part of the first intron."
/translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKN
YGTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNE
RHIPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRL
LNGQPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQV
IQELSLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTA
HSVVQVLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSY
VGAQCPTNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNL
EKPLEQPENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFL
GQQLTQVGVMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFL
GKTIAELPLFLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCA
SSSTSMALSVGPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWAD
VEPGEISCTSSNTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLR
ARRKE"
/standard_name="mini-white"
protein_bind 6508..6602
/bound_moiety="GAL4"
/note="five tandem copies of the ""ScaI site"" 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
/standard_name="5X UAS"
protein_bind 6636..6730
/bound_moiety="GAL4"
/note="five tandem copies of the ""ScaI site"" 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
/standard_name="5X UAS"
regulatory 6761..6999
/note="Geneious type: promoter"
/note="Drosophila melanogaster hsp70Bb promoter"
/standard_name="hsp70 promoter"
primer_bind 6946..6967
/note="Drosophila Hsp70 promoter, forward primer"
/standard_name="pCasper-hs"
intron 7014..7080
/note="modified Drosophila myosin heavy chain intron 16
(Pfeiffer et al., 2010)"
/standard_name="Mhc intron"
gene 7359..8087
/standard_name="GFP"
intron 8165..8230
/note="SV40 (simian virus 40) small t antigen intron"
/standard_name="small t intron"
CDS 8360..8380
/codon_start=1
/product="nuclear localization signal of SV40 (simian
virus 40) large T antigen"
/translation="PKKKRKV"
/standard_name="SV40 NLS"
misc_feature 8652..>8668
/note="Geneious type: polyA_signal"
/note="SV40 polyadenylation signal"
/standard_name="SV40 poly(A) signal"
misc_feature 8675..8848
/note="Geneious type: Editing History Replacement"
/standard_name="TGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAA
AGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTAT
CATGTCTGGATCGATCTGGCCGGCCGTTTAAACGAATTC"
primer_bind 8675..8694
/standard_name="B2 3'F1"
misc_feature 8695..8708
/standard_name="TaG-EM Barcode"
primer_bind complement(8720..8748)
/standard_name="SV40_pre_R "
primer_bind complement(8808..8828)
/standard_name="SV40_post_R "
primer_bind complement(8863..8881)
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
/standard_name="pBRforEco"
ORIGIN
1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag
2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga
3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
6481 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct
6541 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
6601 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt
6661 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
6721 gtcctccgag cggagactct agcgctagcg acgtcgagcg ccggagtata aatagaggcg
6781 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa
6841 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt
6901 taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc
6961 tgccaagaag taattattga atacaagaag agaactctga atagatctaa aaggtaggtt
7021 caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc tttacttcag
7081 gcggccgcgg ctcgagaatc aaaatgggca acaaatgctg cagcaagcga caggatcagg
7141 aactggcact ggcctatccc actgggggct acaagaaatc cgactacacc tttggccaga
7201 cgcacatcaa cagcagcggc ggcggcaaca tgggcggcgt tcttggccag aagcataaca
7261 acggtggctc gctggactcg cgctacacgc ccgatcccaa tcatcggggt ccgttgaaaa
7321 tcggcggaaa gggcggcgtt gacatcatca gaccacgcgg atccatgtcc aaaggtgaag
7381 aactgtttac cggagtagtc ccgatattgg ttgaactcga cggcgatgtc aacggtcata
7441 aattcagtgt gtccggcgag ggtgagggcg acgccacata cggtaagctg acgttgaagt
7501 tcatatgcac cacgggcaag ctgcccgtgc catggccgac gttggtcacg acgctgacgt
7561 atggtgtcca gtgtttcagc cgttaccccg atcatatgaa gcagcatgac tttttcaagt
7621 cggcgatgcc ggagggatac gttcaagaga ggaccatttt cttcaaggat gacggcaact
7681 ataagacgcg agcggaggtg aaatttgaag gcgacacact cgttaaccgt attgagttga
7741 agggcattga ttttaaggag gatggcaaca ttctgggcca taagttggaa tacaactaca
7801 actcgcataa tgtgtatata atggcagata agcagaaaaa cggaataaag gttaacttca
7861 agattcgcca caacatagag gacggttccg tgcaacttgc agatcattac caacagaaca
7921 cacccattgg agatggccca gttctcttgc cagacaatca ctacctttcc acacagtccg
7981 cgttgagcaa ggaccccaat gaaaagcggg accacatggt gttgctggag tttgtgaccg
8041 cagctggtat tacacacggc atggatgagc tctacaagta atctagagga tctttgtgaa
8101 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
8161 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
8221 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
8281 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
8341 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
8401 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
8461 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
8521 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
8581 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
8641 ttgttgttgt taacttgttt attgcagctt ataacttcca acaaccggaa gtgannnnnn
8701 nnnnnnnntg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
8761 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
8821 gatctggccg gccgtttaaa cgaattcttt gaagacgaaa gggcctcgtg atacgcctat
8881 ttttataggt taatgtcatg ataataatgg tttctta
//
