function varargout = LineScanPlotter(varargin) % LINESCANPLOTTER MATLAB code for LineScanPlotter.fig % LINESCANPLOTTER, by itself, creates a new LINESCANPLOTTER or raises the existing % singleton*. % % H = LINESCANPLOTTER returns the handle to a new LINESCANPLOTTER or the handle to % the existing singleton*. % % LINESCANPLOTTER('CALLBACK',hObject,eventData,handles,...) calls the local % function named CALLBACK in LINESCANPLOTTER.M with the given input arguments. % % LINESCANPLOTTER('Property','Value',...) creates a new LINESCANPLOTTER or raises the % existing singleton*. Starting from the left, property value pairs are % applied to the GUI before LineScanPlotter_OpeningFcn gets called. An % unrecognized property name or invalid value makes property application % stop. All inputs are passed to LineScanPlotter_OpeningFcn via varargin. % % *See GUI Options on GUIDE's Tools menu. Choose "GUI allows only one % instance to run (singleton)". % % See also: GUIDE, GUIDATA, GUIHANDLES % Edit the above text to modify the response to help LineScanPlotter % Last Modified by GUIDE v2.5 02-Oct-2017 13:45:58 % Begin initialization code - DO NOT EDIT gui_Singleton = 1; gui_State = struct('gui_Name', mfilename, ... 'gui_Singleton', gui_Singleton, ... 'gui_OpeningFcn', @LineScanPlotter_OpeningFcn, ... 'gui_OutputFcn', @LineScanPlotter_OutputFcn, ... 'gui_LayoutFcn', [] , ... 'gui_Callback', []); if nargin && ischar(varargin{1}) gui_State.gui_Callback = str2func(varargin{1}); end if nargout [varargout{1:nargout}] = gui_mainfcn(gui_State, varargin{:}); else gui_mainfcn(gui_State, varargin{:}); end % End initialization code - DO NOT EDIT % --- Executes just before LineScanPlotter is made visible. function LineScanPlotter_OpeningFcn(hObject, eventdata, handles, varargin) % This function has no output args, see OutputFcn. % hObject handle to figure % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) % varargin command line arguments to LineScanPlotter (see VARARGIN) % Choose default command line output for LineScanPlotter handles.output = hObject; % Update handles structure guidata(hObject, handles); % This sets up the initial plot - only do when we are invisible % so window can get raised using LineScanPlotter. if strcmp(get(hObject,'Visible'),'off') plot(nan(5)); end % UIWAIT makes LineScanPlotter wait for user response (see UIRESUME) % uiwait(handles.figure1); % --- Outputs from this function are returned to the command line. function varargout = LineScanPlotter_OutputFcn(hObject, eventdata, handles) % varargout cell array for returning output args (see VARARGOUT); % hObject handle to figure % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) % Get default command line output from handles structure varargout{1} = handles.output; % --- Executes on button press in pushbutton1. function pushbutton1_Callback(hObject, eventdata, handles) % hObject handle to pushbutton1 (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) axes(handles.axes1); cla; % % dataDir = '~/Dropbox/CurrentData/CalciumImaging/'; dataDir = 'C:\Users\scientifica\Documents\ScanImageData\'; popup_selection = handles.popupmenu1.String(handles.popupmenu1.Value); cd([dataDir,popup_selection{1}]); %load file to plot cellID = handles.edit1.String{1}; trialsToPull = eval(handles.edit2.String{1}); accum_data = []; for tt = 1:length(trialsToPull) currentTrial_str = num2str(trialsToPull(tt)); switch length(currentTrial_str) case 1 fileName = [cellID,'_0000',currentTrial_str]; case 2 fileName = [cellID,'_000',currentTrial_str]; case 3 fileName = [cellID,'_00',currentTrial_str]; end if tt == 1 metaFileName= fileName; end [~, pmtData, ~, roiGroup] = readLineScanDataFiles_riekeLab(fileName,metaFileName); noROIs = size(roiGroup.rois,2); plotLen = size(pmtData,1) / noROIs; for ii = 1:noROIs plotStart = plotLen * (ii-1) + 1; plotEnd = plotLen * ii; %Picking channel 1 out of pmtData: accum_data(tt,:,ii) = mean(squeeze(pmtData(plotStart:plotEnd,1,:))); end end %plot new data: hold on; colors = hsv(noROIs); for ii = 1:noROIs plot(mean(accum_data(:,:,ii),1),'Color',colors(ii,:)) end title('Channel 1') % -------------------------------------------------------------------- function FileMenu_Callback(hObject, eventdata, handles) % hObject handle to FileMenu (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) % -------------------------------------------------------------------- function OpenMenuItem_Callback(hObject, eventdata, handles) % hObject handle to OpenMenuItem (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) file = uigetfile('*.fig'); if ~isequal(file, 0) open(file); end % -------------------------------------------------------------------- function PrintMenuItem_Callback(hObject, eventdata, handles) % hObject handle to PrintMenuItem (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) printdlg(handles.figure1) % -------------------------------------------------------------------- function CloseMenuItem_Callback(hObject, eventdata, handles) % hObject handle to CloseMenuItem (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) selection = questdlg(['Close ' get(handles.figure1,'Name') '?'],... ['Close ' get(handles.figure1,'Name') '...'],... 'Yes','No','Yes'); if strcmp(selection,'No') return; end delete(handles.figure1) % --- Executes on selection change in popupmenu1. function popupmenu1_Callback(hObject, eventdata, handles) % hObject handle to popupmenu1 (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) % Hints: contents = get(hObject,'String') returns popupmenu1 contents as cell array % contents{get(hObject,'Value')} returns selected item from popupmenu1 % --- Executes during object creation, after setting all properties. function popupmenu1_CreateFcn(hObject, eventdata, handles) % hObject handle to popupmenu1 (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles empty - handles not created until after all CreateFcns called % Hint: popupmenu controls usually have a white background on Windows. % See ISPC and COMPUTER. if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) set(hObject,'BackgroundColor','white'); end %populate popup menu 1 with data directory names % % dataDir = '~/Dropbox/CurrentData/CalciumImaging/'; dataDir = 'C:\Users\scientifica\Documents\ScanImageData\'; cd(dataDir); tempDir = dir; tempInd = find(vertcat(tempDir.isdir)); directories = tempDir(tempInd); directories = directories(~cellfun('isempty', {directories.date})); %exclude invalid entries nameList = {}; ct = 0; for dd = 1:length(directories) if strcmp(directories(dd).name,'.') continue elseif strcmp(directories(dd).name,'..') continue end ct = ct + 1; nameList{ct} = directories(dd).name; end set(hObject, 'String', nameList); function edit1_Callback(hObject, eventdata, handles) % hObject handle to edit1 (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) % Hints: get(hObject,'String') returns contents of edit1 as text % str2double(get(hObject,'String')) returns contents of edit1 as a double % --- Executes during object creation, after setting all properties. function edit1_CreateFcn(hObject, eventdata, handles) % hObject handle to edit1 (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles empty - handles not created until after all CreateFcns called % Hint: edit controls usually have a white background on Windows. % See ISPC and COMPUTER. if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) set(hObject,'BackgroundColor','white'); end function edit2_Callback(hObject, eventdata, handles) % hObject handle to edit2 (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles structure with handles and user data (see GUIDATA) % Hints: get(hObject,'String') returns contents of edit2 as text % str2double(get(hObject,'String')) returns contents of edit2 as a double % --- Executes during object creation, after setting all properties. function edit2_CreateFcn(hObject, eventdata, handles) % hObject handle to edit2 (see GCBO) % eventdata reserved - to be defined in a future version of MATLAB % handles empty - handles not created until after all CreateFcns called % Hint: edit controls usually have a white background on Windows. % See ISPC and COMPUTER. if ispc && isequal(get(hObject,'BackgroundColor'), get(0,'defaultUicontrolBackgroundColor')) set(hObject,'BackgroundColor','white'); end