https://gitlab.com/tgwgraham/papa_paper_plasmids
Tip revision: 984803582c0023a9fe975ec6d41be3d723c2737b authored by Thomas Graham on 08 July 2022, 01:06:31 UTC
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Tip revision: 9848035
ptg771-ef1alpha-snapf-mar.gb
LOCUS pTG771_EF1alpha-SNAPf- 10248 bp ds-DNA circular 31-AUG-2021
DEFINITION .
KEYWORDS "creator:Gina Dailey - Darzacq/Tjian Lab" "marker:AmpR, PuroR"
FEATURES Location/Qualifiers
primer complement(108..126)
/label="oTG1552_pBS_diffusion_substrate_300bp"
/note="sequence: ctctagcttcccggcaaca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS complement(650..718)
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter complement(719..823)
/label="AmpR promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(802..823)
/label="oTG1179_FRT_AmpR_5’"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCcgcggaacccctatttgtttat"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(821..839)
/label="oTG1553_pBS_diffusion_substrate_1kb"
/note="sequence: ttttcggggaaatgtgcgc"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
rep_origin complement(849..1303)
/label="f1 ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 1441..1460
/label="oTG1336_M13F_extended"
/note="sequence: GTTGTAAAACGACGGCCAGT"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind 1443..1460
/label="M13-fwd"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 1444..1460
/label="M13 fwd"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
misc_feature 1590..1602
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 1637..1652
/label="IR (inverted repeat) truncated?"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
promoter 2071..2570
/label="PGK promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(2131..2150)
/label="oTG1779_PGK_gPCR_rev"
/note="sequence: acttgtgtagcgccaagtgc"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
CDS 2592..3191
/label="PuroR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
polyA_signal 3224..3448
/label="bGH poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 3991..4010
/label="oTG1576_3xFlag-Halo_upstream_F"
/note="sequence: CCGGCGCCTACTCTAGCCAC"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 3991..4034
/label="oTG1580_Kozak_SNAPf_F"
/note="sequence: CCGGCGCCTACTCTAGCCACCatggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 4012..4033
/label="oTG1577_Halo_linker_SNAPf_F"
/note="sequence: CGACGCTCGAGATTTCCGGCggtagtggtaccggttctATGGACAAAGACTGCGAAATGA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 4012..4034
/label="oTG1562_FKBP-SNAPf_F2"
/note="sequence: atggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
CDS 4012..4557
/label="SNAPf"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
gene 4012..4557
/label="SNAP26f"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
source 4012..4557
/label="source"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS 4012..7330
/label="Translation 4012-7330"
primer complement(4099..4119)
/label="oTG1722_SNAPf_v2_int_R"
/note="sequence: tttgcccaggaagatgatacg"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4538..4557)
/label="oTG1731_SNAPf_stop_BamHI_R"
/note="sequence: TCAGTTATCTAGATCCGGTGGATCCTTAacccagcccaggcttgccca"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4538..4557)
/label="oTG1581_SNAPf_3’_R"
/note="sequence: acccagcccaggcttgccca"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(4538..4558)
/label="oTG1563_FKBP-SNAPf_R2"
/note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(4538..4559)
/label="oTG1578_SNAPf_noNLS_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCacccagcccaggcttgccca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 4549..4587
/label="oTG1752_SNAP-6xGA_F"
/note="sequence: GGGCTGGGTGGTAGTGGTACCggagctggtgcaggtgct"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4587..4605)
/label="oTG1753_6xGA-Halo_R"
/note="sequence: GATTTCTGCCATAGAACCGGTACCtgcacctgctcctgctcca"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 4606..7305
/label="Mouse AR"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(4652..4671)
/label="oTG1796_mAR_22_R"
/note="sequence: CGCTCCTCGATAGGTCTTGG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4686..4704
/label="oTG1795_mAR_FQNLF_AQNAA_F"
/note="sequence: gccCAGAATgcggccCAGAGCGTGCGCGAAGCG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(6242..6261)
/label="oTG1751_mAR_552_R"
/note="sequence: TCCGTAGTGACAGCCAGAAG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 6264..6282
/label="oTG1750_mAR_A553D_F"
/note="sequence: gatCTCACTTGTGGCAGCTGC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(6276..6297)
/label="oTG1792_mAR_564_R"
/note="sequence: TTTGAAGAAGACCTTGCAGCTG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 6301..6318
/label="oTG1790_mAR_R565A_F"
/note="sequence: gctGCCGCTGAAGGGAAACAG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 6301..6318
/label="oTG1791_mAR_R565K_F"
/note="sequence: aagGCCGCTGAAGGGAAACAG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(6304..6330)
/label="oTG1794_mAR_578_R"
/note="sequence: ACATAGATACTTCTGTTTCCCTTCAGC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 6336..6365
/label="oTG1793_mAR_A576T_S577T_F"
/note="sequence: accaccAGAAACGATTGTACCATTGATAAATTTCG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(6569..6587)
/label="oTG1781_mAR_seq_R"
/note="sequence: TCAATGGCTTCCAGGACGT"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(7281..7298)
/label="oTG1600_AR-6xGA-SNAP_R"
/note="sequence: TCTTTGTCCATAGAACCGGTACCtgcacctgctcctgctccagcacctgcaccagctccCTGGGTGTGGAAATAGATGGGC"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 7281..7303
/label="oTG1780_mAR_gPCR_fwd"
/note="sequence: GCCCATCTATTTCCACACACAGT"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 7304..7328
/label="oTG967_pHalo-Stop_F"
/note="sequence: TAGGATCCACCGGATCTAGATAACT"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 7305..7328
/label="oTG967_pHalo-Stop_F"
/note="sequence: TAGGATCCACCGGATCTAGATAACT"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 7306..7328
/label="oTG967_pHalo-Stop_F"
/note="sequence: TAGGATCCACCGGATCTAGATAACT"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(7311..7339)
/label="oTG1579_3xNLS_downstream_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTG"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 7320..7330
/label="stop codons"
/ApEinfo_revcolor=#ff0000
/ApEinfo_fwdcolor=#ff0000
misc_feature 7407..7407
/label="add_before"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 7407..7424
/label="MCS"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
polyA_signal 7540..7661
/label="SV40 poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(7633..7661)
/label="oTG885_SV40_pA_F"
/note="sequence: TAAGATACATTGATGAGTTTGGACAAACC"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature complement(8421..8439)
/label="IR (inverted repeat)"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
misc_feature complement(8443..8455)
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind complement(8946..8962)
/label="M13 rev"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
protein_bind 8970..8986
/label="lac operator"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
promoter complement(8994..9000)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(9001..9018)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(9012..9033)
/label="oTG980_Vector-Fwd-GA"
/note="sequence: ccggatgacagtgacgaggagaactgaACCCCAGGCTTTacacTTTATG"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
promoter complement(9019..9024)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
protein_bind 9039..9060
/label="CAP binding site"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
primer 9091..9111
/label="oTG1156_mCherry-LacI-Rev"
/note="sequence: AAGCTTCCATTCGGGAAACCTGTCGtgCCAG"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 9111..9131
/label="oTG1089_Rev-LacI-NLS-NOSTOP-GA"
/note="sequence: AACCTTCCTCTTCTTCTTAGGAGGCTGCATTAATGAATCGGCCA"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 9111..9132
/label="oTG1084_Rev-LacI-GA"
/note="sequence: TCAAACCTTCCTCTtcTTcTTaGgagGCTGCATTAATGAATCGGCCAA"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 9168..9185
/label="oTG775_pET_BbvCI_insert_seq"
/note="sequence: TTCCGCTTCCTCGCTCAC"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
rep_origin complement(9348..9935)
/label="ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 9989..10012
/label="oTG1180_AmpR_seq"
/note="sequence: ACGTTAAGGGATTTTGGTCATGAG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(10075..10105)
/label="oTG1177_FRT_AmpR_3’flanking"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer complement(10075..10105)
/label="oTG1554_pBS_diffusion_substrate_3kb"
/note="sequence: ctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 10076..10105
/label="oTG1182_E75_AmpR_F"
/note="sequence: gactaactgccactcgcagcgcgcagatcgAAGTATATATGAGTAAACTTGGTCTGACAG"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer 10105..10129
/label="oTG1549_pBS_849_adapter"
/note="sequence: GCTGTGTCTTATTACACACAGTCGttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
CDS complement(join(10106..10248,1..649))
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 10106..10129
/label="oTG1176_FRT_AmpR_3’"
/note="sequence: CTATTCTCTAGAAAGTATAGGAACTTCttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(10152..10174)
/label="oTG1550_pBS_diffusion_substrate_100bp"
/note="sequence: caggcaactatggatgaacgaaa"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC
61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG
121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA
181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA
241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA
301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA
361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA
421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG
481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG
541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG
601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG
661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC
721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT
901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA
961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA
1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA
1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG
1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA
1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG
1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG
1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC
1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG
1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG
1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG
1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT
1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA
1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT
1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT
1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT
1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA
1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC
2041 TGGGGGGATA CGGGGAAAAG GCGAACGCGT gggtagggga ggcgcttttc ccaaggcagt
2101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
2161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
2221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
2281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
2341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
2401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
2461 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
2521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
2581 caAGCTTCAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC
2641 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG
2701 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG
2761 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA
2821 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG
2881 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC
2941 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA
3001 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC
3061 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA
3121 CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC
3181 CCGGTGCCTG AGCGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AACGCGATCG CTCCGGTGCC
3481 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC
3541 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC
3601 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG
3661 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC
3721 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC
3781 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA
3841 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC
3901 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT
3961 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCCAC Catggacaaa
4021 gactgcgaaa tgaagcgcac caccctggat agccctctgg gcaagctgga actgtctggg
4081 tgcgaacagg gcctgcaccg tatcatcttc ctgggcaaag gaacatctgc cgccgacgcc
4141 gtggaagtgc ctgccccagc cgccgtgctg ggcggaccag agccactgat gcaggccacc
4201 gcctggctca acgcctactt tcaccagcct gaggccatcg aggagttccc tgtgccagcc
4261 ctgcaccacc cagtgttcca gcaggagagc tttacccgcc aggtgctgtg gaaactgctg
4321 aaagtggtga agttcggaga ggtcatcagc tacagccacc tggccgccct ggccggcaat
4381 cccgccgcca ccgccgccgt gaaaaccgcc ctgagcggaa atcccgtgcc cattctgatc
4441 ccctgccacc gggtggtgca gggcgacctg gacgtggggg gctacgaggg cgggctcgcc
4501 gtgaaagagt ggctgctggc ccacgagggc cacagactgg gcaagcctgg gctgggtggt
4561 agtggtaccg gagctggtgc aggtgctgga gcaggagcag gtgcaATGGA GGTGCAGTTA
4621 GGGCTGGGAA GGGTCTACCC ACGGCCCCCA TCCAAGACCT ATCGAGGAGC GTTCCAGAAT
4681 CTGTTCCAGA GCGTGCGCGA AGCGATCCAG AACCCGGGCC CCAGGCACCC TGAGGCCGCT
4741 AACATAGCAC CTCCCGGCGC CTGTTTACAG CAGAGGCAGG AGACTAGCCC CCGGCGGCGG
4801 CGGCGGCAGC AGCACACTGA GGATGGTTCT CCTCAAGCCC ACATCAGAGG CCCCACAGGC
4861 TACCTGGCCC TGGAGGAGGA ACAGCAGCCT TCACAGCAGC AGGCAGCCTC CGAGGGCCAC
4921 CCTGAGAGCA GCTGCCTCCC CGAGCCTGGG GCGGCCACCG CTCCTGGCAA GGGGCTGCCG
4981 CAGCAGCCAC CAGCTCCTCC AGATCAGGAT GACTCAGCTG CCCCATCCAC GTTGTCCCTG
5041 CTGGGCCCCA CTTTCCCAGG CTTAAGCAGC TGCTCCGCCG ACATTAAAGA CATTTTGAAC
5101 GAGGCCGGCA CCATGCAACT TCTTCAGCAG CAGCAACAAC AGCAGCAGCA CCAACAGCAG
5161 CACCAACAGC ACCAACAGCA GCAGGAGGTA ATCTCCGAAG GCAGCAGCGC AAGAGCCAGG
5221 GAGGCCACGG GGGCTCCCTC TTCCTCCAAG GATAGTTACC TAGGGGGCAA TTCAACCATA
5281 TCTGACAGTG CCAAGGAGTT GTGTAAAGCA GTGTCTGTGT CCATGGGATT GGGTGTGGAA
5341 GCATTGGAAC ATCTGAGTCC AGGGGAACAG CTTCGGGGAG ACTGCATGTA CGCGTCGCTC
5401 CTGGGAGGTC CACCCGCGGT GCGTCCCACT CCTTGTGCGC CGCTGCCCGA ATGCAAAGGT
5461 CTTCCCCTGG ACGAAGGCCC AGGCAAAAGC ACTGAAGAGA CTGCTGAGTA TTCCTCTTTC
5521 AAGGGAGGTT ACGCCAAAGG ATTGGAAGGT GAGAGCTTGG GGTGCTCTGG CAGCAGTGAA
5581 GCAGGTAGCT CTGGGACACT TGAGATCCCG TCCTCTCTGT CTCTGTATAA ATCTGGAGCA
5641 CTAGACGAGG CAGCAGCATA CCAGAATCGC GACTACTACA ACTTTCCGCT GGCTCTGTCC
5701 GGGCCGCCGC ACCCCCCGCC CCCTACCCAT CCACACGCCC GTATCAAGCT GGAGAACCCA
5761 TTGGACTACG GCAGCGCCTG GGCTGCGGCG GCAGCGCAAT GCCGCTATGG GGACTTGGGT
5821 AGTCTACATG GAGGGAGTGT AGCCGGGCCC AGCACTGGAT CGCCCCCAGC CACCACCTCT
5881 TCTTCCTGGC ATACTCTCTT CACAGCTGAA GAAGGCCAAT TATATGGGCC AGGAGGCGGG
5941 GGCGGCAGCA GCAGCCCAAG CGATGCCGGG CCTGTAGCCC CCTATGGCTA CACTCGGCCC
6001 CCTCAGGGGC TGACAAGCCA GGAGAGTGAC TACTCTGCCT CCGAAGTGTG GTATCCTGGT
6061 GGAGTTGTGA ACAGAGTACC CTATCCCAGT CCCAATTGTG TCAAAAGTGA AATGGGACCT
6121 TGGATGGAGA ACTACTCCGG ACCTTATGGG GACATGCGTT TGGACAGTAC CAGGGACCAT
6181 GTTTTACCCA TCGACTATTA CTTTCCACCC CAGAAGACCT GCCTGATCTG TGGAGATGAA
6241 GCTTCTGGCT GTCACTACGG AGCTCTCACT TGTGGCAGCT GCAAGGTCTT CTTCAAAAGA
6301 GCCGCTGAAG GGAAACAGAA GTATCTATGT GCCAGCAGAA ACGATTGTAC CATTGATAAA
6361 TTTCGGAGGA AAAATTGCCC ATCTTGTCGT CTCCGGAAAT GTTATGAAGC AGGGATGACT
6421 CTGGGAGCTC GTAAGCTGAA GAAACTTGGA AATCTAAAAC TACAGGAGGA AGGAGAAAAC
6481 TCCAATGCTG GCAGCCCCAC TGAGGACCCA TCCCAGAAGA TGACTGTATC ACACATTGAA
6541 GGCTATGAAT GTCAGCCTAT CTTTCTTAAC GTCCTGGAAG CCATTGAGCC AGGAGTGGTG
6601 TGTGCCGGAC ATGACAACAA CCAACCAGAT TCCTTTGCTG CCTTGTTATC TAGCCTCAAT
6661 GAGCTTGGAG AGAGGCAGCT TGTGCATGTG GTCAAGTGGG CCAAGGCCTT GCCTGGCTTC
6721 CGCAACTTGC ATGTGGATGA CCAGATGGCG GTCATTCAGT ATTCCTGGAT GGGACTGATG
6781 GTATTTGCCA TGGGTTGGCG GTCCTTCACT AATGTCAACT CCAGGATGCT CTACTTTGCA
6841 CCTGACTTGG TTTTCAATGA GTACCGCATG CACAAGTCTC GGATGTACAG CCAGTGTGTG
6901 AGGATGAGGC ACCTGTCTCA AGAGTTTGGA TGGCTCCAAA TAACCCCCCA GGAATTCCTG
6961 TGCATGAAAG CACTGCTGCT CTTCAGCATT ATTCCAGTGG ATGGGCTGAA AAATCAAAAA
7021 TTCTTTGATG AACTTCGAAT GAACTACATC AAGGAACTCG ATCGCATCAT TGCATGCAAA
7081 AGAAAGAATC CCACATCCTG CTCAAGGCGC TTCTACCAGC TCACCAAGCT CCTGGATTCT
7141 GTGCAGCCTA TTGCAAGAGA GCTGCATCAG TTCACTTTTG ACCTGCTAAT CAAGTCCCAT
7201 ATGGTGAGCG TGGACTTTCC TGAAATGATG GCAGAGATCA TCTCTGTGCA AGTGCCCAAG
7261 ATCCTTTCTG GGAAAGTCAA GCCCATCTAT TTCCACACAC AGTGAGGATC CACCGGATCT
7321 AGATAACTGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC
7381 CTCCCACACC TCCCCCTGAA CCTGAACGCG GCCGCtggGT CGACAATCAA CCTCTGGATT
7441 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG
7501 GATACGCTGC TTTAATGCCT TTGTATCATG CGTTAACTAA ACTTGTTTAT TGCAGCTTAT
7561 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG
7621 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GAATTGACTC
7681 AAATGATGTC AATTAGTCTA TCAGAAGCTC ATCTGGTCTC CCTTCCGGGG GACAAGACAT
7741 CCCTGTTTAA TATTTAAACA GCAGTGTTCC CAAACTGGGT TCTTATATCC CTTGCTCTGG
7801 TCAACCAGGT TGCAGGGTTT CCTGTCCTCA CAGGAACGAA GTCCCTAAAG AAACAGTGGC
7861 AGCCAGGTTT AGCCCCGGAA TTGACTGGAT TCCTTTTTTA GGGCCCATTG GTATGGCTTT
7921 TTCCCCGTAT CCCCCCAGGT GTCTGCAGGC TCAAAGAGCA GCGAGAAGCG TTCAGAGGAA
7981 AGCGATCCCG TGCCACCTTC CCCGTGCCCG GGCTGTCCCC GCACGCTGCC GGCTCGGGGA
8041 TGCGGGGGGA GCGCCGGACC GGAGCGGAGC CCCGGGCGGC TCGCTGCTGC CCCCTAGCGG
8101 GGGAGGGACG TAATTACATC CCTGGGGGCT TTGGGGGGGG GCTGTCCCTG ATATCTATAA
8161 CAAGAAATAT ATATATAATA AGTTATCACG TAAGTAGAAC ATGAAATAAC AATATAATTA
8221 TCGTATGAGT TAATCTTAAA GTCACGTAAA AGATAATCAT GCGTCATTTT GACTCACGCG
8281 GTCGTTATAG TTCAAAATCA GTGACACTTA CCGCATTGAC AAGCACGCCT CACGGGAGCT
8341 CCAAGCGGCG ACTGAGATGT CCTAAATGCA CAGCGACGGA TTCGCGCTAT TTAGAAAGAG
8401 AGAGCAATAT TTCAAGAATG CATGCGTCAA TTTTACGCAG ACTATCTTTC TAGGGTTAAT
8461 CTAGCTGCAT CAGGATCATA TCGTCGGGTC TTTTTTCCGG CTCAGTCATC GCCCAAGCTG
8521 GCGCTATCTG GGCATCGGGG AGGAAGAAGC CCGTGCCTTT TCCCGCGAGG TTGAAGCGGC
8581 ATGGAAAGAG TTTGCCGAGG ATGACTGCTG CTGCATTGAC GTTGAGCGAA AACGCACGTT
8641 TACCATGATG ATTCGGGAAG GTGTGGCCAT GCACGCCTTT AACGGTGAAC TGTTCGTTCA
8701 GGCCACCTGG GATACCAGTT CGTCGCGGCT TTTCCGGACA CAGTTCCGGA TGGTCAGCCC
8761 GAAGCGCATC AGCAACCCGA ACAATACCGG CGACAGCCGG AACTGCCGTG CCGGTGTGCA
8821 GATTAATGAC AGCGGTGCGG CGCTGGGATA TTACGTCAGC GAGGACGGGT ATCCTGGCTG
8881 GATGCCGCAG AAATGGACAT GGATACCCCG TGAGTTACCC GGCGGGCGCG CTTGGCGTAA
8941 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA
9001 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
9061 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA
9121 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG
9181 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG
9241 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
9301 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
9361 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
9421 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG
9481 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
9541 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
9601 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
9661 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
9721 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC
9781 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAGAG
9841 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA
9901 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG
9961 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA
10021 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA
10081 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG
10141 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA
10201 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAG
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