https://gitlab.com/tgwgraham/papa_paper_plasmids
Tip revision: 984803582c0023a9fe975ec6d41be3d723c2737b authored by Thomas Graham on 08 July 2022, 01:06:31 UTC
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Tip revision: 9848035
ptg746-ef1alpha-3xflag-halo-snapf.gb
LOCUS pTG746_EF1alpha-3xFlag- 8487 bp ds-DNA circular 30-NOV-2020
DEFINITION .
KEYWORDS "creator:Gina Dailey - Darzacq/Tjian Lab" "marker:AmpR, PuroR"
FEATURES Location/Qualifiers
primer complement(108..126)
/label="oTG1552_pBS_diffusion_substrate_300bp"
/note="sequence: ctctagcttcccggcaaca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS complement(650..718)
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter complement(719..823)
/label="AmpR promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(802..823)
/label="oTG1179_FRT_AmpR_5’"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCcgcggaacccctatttgtttat"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(821..839)
/label="oTG1553_pBS_diffusion_substrate_1kb"
/note="sequence: ttttcggggaaatgtgcgc"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
rep_origin complement(849..1303)
/label="f1 ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 1441..1460
/label="oTG1336_M13F_extended"
/note="sequence: GTTGTAAAACGACGGCCAGT"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind 1443..1460
/label="M13-fwd"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 1444..1460
/label="M13 fwd"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
misc_feature 1590..1602
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 1637..1652
/label="IR (inverted repeat) truncated?"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
promoter 2071..2570
/label="PGK promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS 2592..3191
/label="PuroR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
polyA_signal 3224..3448
/label="bGH poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
CDS 4012..4014
/label="ATG"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 4012..5569
/label="Translation 4012-5569"
CDS 4015..4080
/label="3xFLAG"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 4089..4114
/label="oTG945_60xHalo_F"
/note="sequence: CCGGCTCCGGCAGCGGTAGCATGGCAGAAATCGGTACTGGCTTTC"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 4090..4110
/label="oTG898_H10_Halo_F"
/note="sequence: TTCTCACCATCACCATCACCACCACCACCACCATATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 4090..4110
/label="oTG1573_GSGSKCK_Halo_F"
/note="sequence: GGTAGTGGTAGTAAGTGTAAAATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer 4090..4110
/label="oTG778_5'Halo"
/note="sequence: ATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
CDS 4090..4980
/label="HaloTag®"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 4093..4113
/label="oTG1144_inVitro_60xHalo_F"
/note="sequence: GGCTCCGGCAGCGGTAGCGCAGAAATCGGTACTGGCTTT"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer 4096..4118
/label="oTG1280_60mer_linker_Halo_F"
/note="sequence: GGAGTGGTAGCGGCAGTATTAAGgaaatcggtactggctttccatt"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 4096..4118
/label="oTG995_Halo_linker_F"
/note="sequence: GATCTGGAGGTTCAGGAGGCAGCGGTGGAAGTGAAATCGGTACTGGCTTTCCATT"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer complement(4101..4120)
/label="oTG1172_Halo_int_R2"
/note="sequence: CGAATGGAAAGCCAGTACCG"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(4106..4125)
/label="oTG1214_Halo_int_R_75"
/note="sequence: GGGGTCGAATGGAAAGCCAG"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(4129..4148)
/label="oTG1096_Halo_int_R"
/note="sequence: TCGCCCAGGACTTCCACATA"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(4150..4169)
/label="oTG1173_Halo_int_R3"
/note="sequence: ACATCGACGTAGTGCATGCG"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 4311..4332
/label="oTG1513_Halo_qPCR_F1"
/note="sequence: ACCAGACCTGGGTTATTTCTTC"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer 4451..4470
/label="oTG1208_Halo_int_F"
/note="sequence: AGCGCGTCAAAGGTATTGCA"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(4471..4490)
/label="oTG1514_Halo_qPCR_R1"
/note="sequence: GGGCGGATGAACTCCATAAA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(4629..4650)
/label="oTG1207_Halo_int_R"
/note="sequence: GTCCATCTCGACTTCAGTCAGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 4636..4657
/label="oTG1515_Halo_qPCR_F2"
/note="sequence: GAAGTCGAGATGGACCATTACC"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer complement(4758..4777)
/label="oTG1516_Halo_qPCR_R2"
/note="sequence: GCAGCCAGTCCATGTATTCT"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 4871..4890
/label="oTG1095_Halo_int_F"
/note="sequence: ACTGCAAGGCTGTGGACATC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 4898..4920
/label="oTG979_3'_Halo_seq"
/note="sequence: GTCTGAATCTGCTGCAAGAAGAC"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer complement(4948..4965)
/label="oTG1312_Halo_tubUTR_R"
/note="sequence: cgcgacgcttagtgtgccgcTTACGTCGACAGCCAGCGCGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(4960..4979)
/label="oTG1261_ParB2_Halo_stop_R"
/note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(4960..4979)
/label="oTG1261_ParB2_Halo_stop_R"
/note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(4961..4980)
/label="oTG779_Halo-stop_pETSUMO"
/note="sequence: GCTCCAATTGGGTACCCATGGAGCCGGAAATCTCGAGCGTCG"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer complement(4961..4980)
/label="oTG793_3'Halo_R"
/note="sequence: GCCGGAAATCTCGAGCGTCG"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer 4999..5021
/label="oTG1562_FKBP-SNAPf_F2"
/note="sequence: atggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
gene 4999..5544
/label="SNAP26f"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 4999..5544
/label="SNAPf"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
CDS 4999..5544
/label="Translation 4999-5544"
source 4999..5544
/label="source"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(5525..5544)
/label="oTG1563_FKBP-SNAPf_R2"
/note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 5543..5567
/label="oTG967_pHalo-Stop_F"
/note="sequence: TAGGATCCACCGGATCTAGATAACT"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
misc_feature 5559..5569
/label="stop codons"
/ApEinfo_revcolor=#ff0000
/ApEinfo_fwdcolor=#ff0000
misc_feature 5646..5646
/label="add_before"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 5646..5663
/label="MCS"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
polyA_signal 5779..5900
/label="SV40 poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(5872..5900)
/label="oTG885_SV40_pA_F"
/note="sequence: TAAGATACATTGATGAGTTTGGACAAACC"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature complement(6660..6678)
/label="IR (inverted repeat)"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
misc_feature complement(6682..6694)
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind complement(7185..7201)
/label="M13 rev"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
protein_bind 7209..7225
/label="lac operator"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
promoter complement(7233..7239)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(7240..7257)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(7251..7272)
/label="oTG980_Vector-Fwd-GA"
/note="sequence: ccggatgacagtgacgaggagaactgaACCCCAGGCTTTacacTTTATG"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
promoter complement(7258..7263)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
protein_bind 7278..7299
/label="CAP binding site"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
primer 7330..7350
/label="oTG1156_mCherry-LacI-Rev"
/note="sequence: AAGCTTCCATTCGGGAAACCTGTCGtgCCAG"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 7350..7370
/label="oTG1089_Rev-LacI-NLS-NOSTOP-GA"
/note="sequence: AACCTTCCTCTTCTTCTTAGGAGGCTGCATTAATGAATCGGCCA"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 7350..7371
/label="oTG1084_Rev-LacI-GA"
/note="sequence: TCAAACCTTCCTCTtcTTcTTaGgagGCTGCATTAATGAATCGGCCAA"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 7407..7424
/label="oTG775_pET_BbvCI_insert_seq"
/note="sequence: TTCCGCTTCCTCGCTCAC"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
rep_origin complement(7587..8174)
/label="ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 8228..8251
/label="oTG1180_AmpR_seq"
/note="sequence: ACGTTAAGGGATTTTGGTCATGAG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(8314..8344)
/label="oTG1554_pBS_diffusion_substrate_3kb"
/note="sequence: ctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer complement(8314..8344)
/label="oTG1177_FRT_AmpR_3’flanking"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer 8315..8344
/label="oTG1182_E75_AmpR_F"
/note="sequence: gactaactgccactcgcagcgcgcagatcgAAGTATATATGAGTAAACTTGGTCTGACAG"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer 8344..8368
/label="oTG1549_pBS_849_adapter"
/note="sequence: GCTGTGTCTTATTACACACAGTCGttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
CDS complement(join(8345..8487,1..649))
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 8345..8368
/label="oTG1176_FRT_AmpR_3’"
/note="sequence: CTATTCTCTAGAAAGTATAGGAACTTCttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(8391..8413)
/label="oTG1550_pBS_diffusion_substrate_100bp"
/note="sequence: caggcaactatggatgaacgaaa"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC
61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG
121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA
181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA
241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA
301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA
361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA
421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG
481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG
541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG
601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG
661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC
721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT
901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA
961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA
1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA
1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG
1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA
1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG
1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG
1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC
1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG
1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG
1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG
1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT
1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA
1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT
1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT
1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT
1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA
1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC
2041 TGGGGGGATA CGGGGAAAAG GCGAACGCGT gggtagggga ggcgcttttc ccaaggcagt
2101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
2161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
2221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
2281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
2341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
2401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
2461 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
2521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
2581 caAGCTTCAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC
2641 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG
2701 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG
2761 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA
2821 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG
2881 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC
2941 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA
3001 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC
3061 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA
3121 CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC
3181 CCGGTGCCTG AGCGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AACGCGATCG CTCCGGTGCC
3481 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC
3541 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC
3601 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG
3661 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC
3721 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC
3781 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA
3841 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC
3901 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT
3961 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCCAC CATGGACTAC
4021 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGACAAG
4081 CTTGGCTCCA TGGCAGAAAT CGGTACTGGC TTTCCATTCG ACCCCCATTA TGTGGAAGTC
4141 CTGGGCGAGC GCATGCACTA CGTCGATGTT GGTCCGCGCG ATGGCACCCC TGTGCTGTTC
4201 CTGCACGGTA ACCCGACCTC CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG
4261 ACCCATCGCT GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA ACCAGACCTG
4321 GGTTATTTCT TCGACGACCA CGTCCGCTTC ATGGATGCCT TCATCGAAGC CCTGGGTCTG
4381 GAAGAGGTCG TCCTGGTCAT TCACGACTGG GGCTCCGCTC TGGGTTTCCA CTGGGCCAAG
4441 CGCAATCCAG AGCGCGTCAA AGGTATTGCA TTTATGGAGT TCATCCGCCC TATCCCGACC
4501 TGGGACGAAT GGCCAGAATT TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC
4561 GGCCGCAAGC TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC GATGGGTGTC
4621 GTCCGCCCGC TGACTGAAGT CGAGATGGAC CATTACCGCG AGCCGTTCCT GAATCCTGTT
4681 GACCGCGAGC CACTGTGGCG CTTCCCAAAC GAGCTGCCAA TCGCCGGTGA GCCAGCGAAC
4741 ATCGTCGCGC TGGTCGAAGA ATACATGGAC TGGCTGCACC AGTCCCCTGT CCCGAAGCTG
4801 CTGTTCTGGG GCACCCCAGG CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA
4861 AGCCTGCCTA ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT GCAAGAAGAC
4921 AACCCGGACC TGATCGGCAG CGAGATCGCG CGCTGGCTGT CGACGCTCGA GATTTCCGGC
4981 ggtagtggta ccggttctat ggacaaagac tgcgaaatga agcgcaccac cctggatagc
5041 cctctgggca agctggaact gtctgggtgc gaacagggcc tgcaccgtat catcttcctg
5101 ggcaaaggaa catctgccgc cgacgccgtg gaagtgcctg ccccagccgc cgtgctgggc
5161 ggaccagagc cactgatgca ggccaccgcc tggctcaacg cctactttca ccagcctgag
5221 gccatcgagg agttccctgt gccagccctg caccacccag tgttccagca ggagagcttt
5281 acccgccagg tgctgtggaa actgctgaaa gtggtgaagt tcggagaggt catcagctac
5341 agccacctgg ccgccctggc cggcaatccc gccgccaccg ccgccgtgaa aaccgccctg
5401 agcggaaatc ccgtgcccat tctgatcccc tgccaccggg tggtgcaggg cgacctggac
5461 gtggggggct acgagggcgg gctcgccgtg aaagagtggc tgctggccca cgagggccac
5521 agactgggca agcctgggct gggtGGATCC ACCGGATCTA GATAACTGAT CATAATCAGC
5581 CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC
5641 CTGAACGCGG CCGCtggGTC GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA
5701 CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT
5761 TGTATCATGC GTTAACTAAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
5821 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
5881 AAACTCATCA ATGTATCTTA TCATGTCTGG AATTGACTCA AATGATGTCA ATTAGTCTAT
5941 CAGAAGCTCA TCTGGTCTCC CTTCCGGGGG ACAAGACATC CCTGTTTAAT ATTTAAACAG
6001 CAGTGTTCCC AAACTGGGTT CTTATATCCC TTGCTCTGGT CAACCAGGTT GCAGGGTTTC
6061 CTGTCCTCAC AGGAACGAAG TCCCTAAAGA AACAGTGGCA GCCAGGTTTA GCCCCGGAAT
6121 TGACTGGATT CCTTTTTTAG GGCCCATTGG TATGGCTTTT TCCCCGTATC CCCCCAGGTG
6181 TCTGCAGGCT CAAAGAGCAG CGAGAAGCGT TCAGAGGAAA GCGATCCCGT GCCACCTTCC
6241 CCGTGCCCGG GCTGTCCCCG CACGCTGCCG GCTCGGGGAT GCGGGGGGAG CGCCGGACCG
6301 GAGCGGAGCC CCGGGCGGCT CGCTGCTGCC CCCTAGCGGG GGAGGGACGT AATTACATCC
6361 CTGGGGGCTT TGGGGGGGGG CTGTCCCTGA TATCTATAAC AAGAAATATA TATATAATAA
6421 GTTATCACGT AAGTAGAACA TGAAATAACA ATATAATTAT CGTATGAGTT AATCTTAAAG
6481 TCACGTAAAA GATAATCATG CGTCATTTTG ACTCACGCGG TCGTTATAGT TCAAAATCAG
6541 TGACACTTAC CGCATTGACA AGCACGCCTC ACGGGAGCTC CAAGCGGCGA CTGAGATGTC
6601 CTAAATGCAC AGCGACGGAT TCGCGCTATT TAGAAAGAGA GAGCAATATT TCAAGAATGC
6661 ATGCGTCAAT TTTACGCAGA CTATCTTTCT AGGGTTAATC TAGCTGCATC AGGATCATAT
6721 CGTCGGGTCT TTTTTCCGGC TCAGTCATCG CCCAAGCTGG CGCTATCTGG GCATCGGGGA
6781 GGAAGAAGCC CGTGCCTTTT CCCGCGAGGT TGAAGCGGCA TGGAAAGAGT TTGCCGAGGA
6841 TGACTGCTGC TGCATTGACG TTGAGCGAAA ACGCACGTTT ACCATGATGA TTCGGGAAGG
6901 TGTGGCCATG CACGCCTTTA ACGGTGAACT GTTCGTTCAG GCCACCTGGG ATACCAGTTC
6961 GTCGCGGCTT TTCCGGACAC AGTTCCGGAT GGTCAGCCCG AAGCGCATCA GCAACCCGAA
7021 CAATACCGGC GACAGCCGGA ACTGCCGTGC CGGTGTGCAG ATTAATGACA GCGGTGCGGC
7081 GCTGGGATAT TACGTCAGCG AGGACGGGTA TCCTGGCTGG ATGCCGCAGA AATGGACATG
7141 GATACCCCGT GAGTTACCCG GCGGGCGCGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT
7201 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT
7261 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC
7321 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG
7381 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
7441 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
7501 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
7561 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
7621 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
7681 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
7741 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT
7801 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
7861 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
7921 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
7981 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT
8041 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAGAGT TGGTAGCTCT TGATCCGGCA
8101 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
8161 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
8221 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
8281 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
8341 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
8401 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
8461 GCCCCAGTGC TGCAATGATA CCGCGAG
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