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Installation Instructions for Ragout ==================================== Availability ------------ Ragout is available for Mac OS and Linux. Bioconda distribitions ---------------------- Ragout binary releases are available through Bioconda: conda install ragout Build Requirements ------------------ * C++ compiler with C++0x support (GCC 4.6+ / Clang 3.2+ / Apple Clang 4.2+) * GNU make * Cmake Runtime Depenencies ------------------- * Python 2.7 * Sibelia [http://github.com/bioinf/Sibelia] * python-networkx == 2.2 * HAL Tools (optionally) [https://github.com/ComparativeGenomicsToolkit/hal] Local installation ------------------ If you don't want to use bioconda release, you can build Ragout repository clone and run it locally without installing into system. To do this, perform: git clone https://github.com/fenderglass/Ragout.git cd Ragout python setup.py build pip install -r requirements.txt --user python scripts/install-sibelia.py This will also build and install Sibelia and all Python dependencies. See below for HAL installation instructions. Once installed, you can invoke Ragout from the cloned directory by using: bin/ragout System installation ------------------- To integrate Ragout into your system, run: git clone https://github.com/fenderglass/Ragout.git cd Ragout python setup.py build python setup.py install This assumes that you already have python-networkx package installed into your system (using the respective package manager). Sibelia / HAL tools should also be installed / integrated separately. HAL Tools --------- HAL alignment produced by Progressive Cactus could be used for synteny blocks decomposition instead of Sibelia (recommended for large genomes). If you want to use HAL alignment as input, you need to install HAL Tools package [https://github.com/ComparativeGenomicsToolkit/hal] as it is described in the manual. Do not forget to properly set PATH and PYTHONPATH environment variables. Troubleshooting --------------- Q: Many compilation errors, possibly with "unrecognized command line option '-std=c++0x'" message: A: Probably your compiler is too old and does not support C++0x. Make sure you have at least GCC 4.6+ / Clang 3.2+ Q: "libstdc++.so.6: version `CXXABI_1.3.5' not found" or similar error when running A: Ensure that the version of libc++ that was used to compile Ragout is similar to one the you currently using. You can specify an extra search path to a specific library by setting "LD_LIBRARY_PATH" variable.