https://gitlab.com/tgwgraham/papa_paper_plasmids
Tip revision: 984803582c0023a9fe975ec6d41be3d723c2737b authored by Thomas Graham on 08 July 2022, 01:06:31 UTC
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Tip revision: 9848035
ptg820-pb-ef1alpha-3xflag-halo-pp3-snapf-3xnls.gb
LOCUS pTG820_PB_EF1alpha-3xFl 9426 bp ds-DNA circular 15-JUL-2021
DEFINITION .
KEYWORDS "creator:Gina Dailey - Tjian Darzacq Lab" "marker:PuroR, AmpR"
FEATURES Location/Qualifiers
CDS complement(156..534)
/label="ISS"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS complement(650..718)
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter complement(719..823)
/label="AmpR promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
rep_origin complement(849..1303)
/label="f1 ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer_bind 1444..1460
/label="M13 fwd"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
promoter 2071..2570
/label="PGK promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS 2592..3191
/label="PuroR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
polyA_signal 3224..3448
/label="bGH poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
regulatory 4006..4015
/label="Kozak sequence"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 4012..6561
/label="Translation 4012-6561"
CDS 4015..4080
/label="3xFLAG"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
CDS 4090..4980
/label="HaloTag®"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
CDS 4981..5253
/label="7"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 5260..5532
/label="8"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 5539..5811
/label="9"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 5818..6363
/label="SNAP-tag®"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
CDS 6379..6402
/label="GDGAGLIN linker"
/ApEinfo_revcolor=#cd4489
/ApEinfo_fwdcolor=#cd4489
CDS 6415..6435
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 6439..6459
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 6463..6483
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
polyA_signal 6718..6839
/label="SV40 poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer_bind complement(8124..8140)
/label="M13 rev"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
protein_bind 8148..8164
/label="lac operator"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
promoter complement(8172..8178)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(8179..8196)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(8197..8202)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
protein_bind 8217..8238
/label="CAP binding site"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
rep_origin complement(8526..9113)
/label="ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
CDS complement(join(9284..9426,1..649))
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC
61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG
121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA
181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA
241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA
301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA
361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA
421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG
481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG
541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG
601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG
661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC
721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT
901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA
961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA
1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA
1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG
1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA
1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG
1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG
1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC
1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG
1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG
1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG
1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT
1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA
1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT
1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT
1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT
1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA
1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC
2041 TGGGGGGATA CGGGGAAAAG GCGAACGCGT gggtagggga ggcgcttttc ccaaggcagt
2101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
2161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
2221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
2281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
2341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
2401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
2461 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
2521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
2581 caAGCTTCAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC
2641 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG
2701 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG
2761 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA
2821 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG
2881 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC
2941 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA
3001 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC
3061 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA
3121 CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC
3181 CCGGTGCCTG AGCGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AACGCGATCG CTCCGGTGCC
3481 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC
3541 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC
3601 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG
3661 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC
3721 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC
3781 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA
3841 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC
3901 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT
3961 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCCAC CATGGACTAC
4021 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGACAAG
4081 CTTGGCTCCA TGGCAGAAAT CGGTACTGGC TTTCCATTCG ACCCCCATTA TGTGGAAGTC
4141 CTGGGCGAGC GCATGCACTA CGTCGATGTT GGTCCGCGCG ATGGCACCCC TGTGCTGTTC
4201 CTGCACGGTA ACCCGACCTC CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG
4261 ACCCATCGCT GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA ACCAGACCTG
4321 GGTTATTTCT TCGACGACCA CGTCCGCTTC ATGGATGCCT TCATCGAAGC CCTGGGTCTG
4381 GAAGAGGTCG TCCTGGTCAT TCACGACTGG GGCTCCGCTC TGGGTTTCCA CTGGGCCAAG
4441 CGCAATCCAG AGCGCGTCAA AGGTATTGCA TTTATGGAGT TCATCCGCCC TATCCCGACC
4501 TGGGACGAAT GGCCAGAATT TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC
4561 GGCCGCAAGC TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC GATGGGTGTC
4621 GTCCGCCCGC TGACTGAAGT CGAGATGGAC CATTACCGCG AGCCGTTCCT GAATCCTGTT
4681 GACCGCGAGC CACTGTGGCG CTTCCCAAAC GAGCTGCCAA TCGCCGGTGA GCCAGCGAAC
4741 ATCGTCGCGC TGGTCGAAGA ATACATGGAC TGGCTGCACC AGTCCCCTGT CCCGAAGCTG
4801 CTGTTCTGGG GCACCCCAGG CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA
4861 AGCCTGCCTA ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT GCAAGAAGAC
4921 AACCCGGACC TGATCGGCAG CGAGATCGCG CGCTGGCTGT CGACGCTCGA GATTTCCGGC
4981 ttaattgaag ttgagaaacc attatacgga gttgaggtat ttgttgggga aactgctcat
5041 ttcgagatcg aactgtcgga acctgacgta catggacagt ggaagttaaa gggccaacca
5101 ttggcagcta gtcctgactg cgagattatt gaagatggta agaagcacat tctgattttg
5161 cacaactgtc aattagggat gacaggagag gtgtcctttc aggccgctaa tacgaagagt
5221 gccgccaact taaaagtgaa agagttacgc agtactagtc ttattgaggt agaaaaacca
5281 ttatatggtg ttgaagtttt cgtgggagag actgctcatt tcgaaattga gttgtcggaa
5341 ccagacgtcc atgggcaatg gaaactgaag ggccaaccgt tagccgcgag ccccgattgt
5401 gaaattattg aggatggaaa gaaacacatc ttgattcttc acaactgcca actgggaatg
5461 actggagaag tctcatttca agcagccaac accaagtcgg ccgcgaacct taaggtaaaa
5521 gaattgcgta gcgtcgacct tattgaagta gagaagcctc tttatggagt ggaagtcttt
5581 gtaggggaaa cggctcactt tgagatcgag ttgtccgaac ctgatgtaca cggccagtgg
5641 aaattaaaag gtcaaccgtt ggccgcgagt cctgattgcg aaattattga agatggtaaa
5701 aagcatatcc ttattttgca caactgtcag ttgggcatga caggcgaagt cagtttccaa
5761 gccgcaaaca ccaaatcggc tgcaaatctg aaggtcaagg aattgcgttc cggttctatg
5821 gacaaagact gcgaaatgaa gcgcaccacc ctggatagcc ctctgggcaa gctggaactg
5881 tctgggtgcg aacagggcct gcaccgtatc atcttcctgg gcaaaggaac atctgccgcc
5941 gacgccgtgg aagtgcctgc cccagccgcc gtgctgggcg gaccagagcc actgatgcag
6001 gccaccgcct ggctcaacgc ctactttcac cagcctgagg ccatcgagga gttccctgtg
6061 ccagccctgc accacccagt gttccagcag gagagcttta cccgccaggt gctgtggaaa
6121 ctgctgaaag tggtgaagtt cggagaggtc atcagctaca gccacctggc cgccctggcc
6181 ggcaatcccg ccgccaccgc cgccgtgaaa accgccctga gcggaaatcc cgtgcccatt
6241 ctgatcccct gccaccgggt ggtgcagggc gacctggacg tggggggcta cgagggcggg
6301 ctcgccgtga aagagtggct gctggcccac gagggccaca gactgggcaa gcctgggctg
6361 ggtGAGATTT CCGGCagcgg agatggcgct ggactgatca acggcCGAGC TGATCCAAAA
6421 AAGAAGAGAA AGGTAGATCC AAAAAAGAAG AGAAAGGTAG ATCCAAAAAA GAAGAGAAAG
6481 GTAGGATCCA CCGGATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGAGGTT
6541 TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAACGCGGC CGCtggGTCG
6601 ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG
6661 CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCG TTAACTAAAC
6721 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT
6781 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT
6841 CATGTCTGGA ATTGACTCAA ATGATGTCAA TTAGTCTATC AGAAGCTCAT CTGGTCTCCC
6901 TTCCGGGGGA CAAGACATCC CTGTTTAATA TTTAAACAGC AGTGTTCCCA AACTGGGTTC
6961 TTATATCCCT TGCTCTGGTC AACCAGGTTG CAGGGTTTCC TGTCCTCACA GGAACGAAGT
7021 CCCTAAAGAA ACAGTGGCAG CCAGGTTTAG CCCCGGAATT GACTGGATTC CTTTTTTAGG
7081 GCCCATTGGT ATGGCTTTTT CCCCGTATCC CCCCAGGTGT CTGCAGGCTC AAAGAGCAGC
7141 GAGAAGCGTT CAGAGGAAAG CGATCCCGTG CCACCTTCCC CGTGCCCGGG CTGTCCCCGC
7201 ACGCTGCCGG CTCGGGGATG CGGGGGGAGC GCCGGACCGG AGCGGAGCCC CGGGCGGCTC
7261 GCTGCTGCCC CCTAGCGGGG GAGGGACGTA ATTACATCCC TGGGGGCTTT GGGGGGGGGC
7321 TGTCCCTGAT ATCTATAACA AGAAATATAT ATATAATAAG TTATCACGTA AGTAGAACAT
7381 GAAATAACAA TATAATTATC GTATGAGTTA ATCTTAAAGT CACGTAAAAG ATAATCATGC
7441 GTCATTTTGA CTCACGCGGT CGTTATAGTT CAAAATCAGT GACACTTACC GCATTGACAA
7501 GCACGCCTCA CGGGAGCTCC AAGCGGCGAC TGAGATGTCC TAAATGCACA GCGACGGATT
7561 CGCGCTATTT AGAAAGAGAG AGCAATATTT CAAGAATGCA TGCGTCAATT TTACGCAGAC
7621 TATCTTTCTA GGGTTAATCT AGCTGCATCA GGATCATATC GTCGGGTCTT TTTTCCGGCT
7681 CAGTCATCGC CCAAGCTGGC GCTATCTGGG CATCGGGGAG GAAGAAGCCC GTGCCTTTTC
7741 CCGCGAGGTT GAAGCGGCAT GGAAAGAGTT TGCCGAGGAT GACTGCTGCT GCATTGACGT
7801 TGAGCGAAAA CGCACGTTTA CCATGATGAT TCGGGAAGGT GTGGCCATGC ACGCCTTTAA
7861 CGGTGAACTG TTCGTTCAGG CCACCTGGGA TACCAGTTCG TCGCGGCTTT TCCGGACACA
7921 GTTCCGGATG GTCAGCCCGA AGCGCATCAG CAACCCGAAC AATACCGGCG ACAGCCGGAA
7981 CTGCCGTGCC GGTGTGCAGA TTAATGACAG CGGTGCGGCG CTGGGATATT ACGTCAGCGA
8041 GGACGGGTAT CCTGGCTGGA TGCCGCAGAA ATGGACATGG ATACCCCGTG AGTTACCCGG
8101 CGGGCGCGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC
8161 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
8221 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG
8281 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
8341 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG
8401 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA
8461 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
8521 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG
8581 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
8641 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
8701 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT
8761 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
8821 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC
8881 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
8941 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA
9001 GTTACCTTCG GAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
9061 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
9121 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT
9181 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT
9241 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA
9301 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG
9361 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
9421 CGCGAG
//