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https://github.com/vejnar/LabxPipe
02 May 2023, 09:53:20 UTC
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    • v0.6.0
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  • f4c2bd6
  • /
  • src
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  • labxpipe_scripts
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  • lxpipe_generate.py
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Tip revision: adf29e860d11a9d1d3ecb06afff81bbf46207d62 authored by vejnar on 19 April 2023, 15:50:13 UTC
Add --suffix to extract command
Tip revision: adf29e8
lxpipe_generate.py
#!/usr/bin/env python3
# -*- coding: utf-8 -*-

#
# Copyright © 2013 Charles E. Vejnar
#
# This Source Code Form is subject to the terms of the Mozilla Public
# License, v. 2.0. If a copy of the MPL was not distributed with this
# file, You can obtain one at https://www.mozilla.org/MPL/2.0/.
#

"""Generate and convert project files"""

import argparse
import json
import os
import sys

import labxdb

def main(argv=None):
    if argv is None:
        argv = sys.argv
    # Started from wrapper?
    prog = os.path.basename(argv[0])
    if len(argv) > 1 and argv[1] == 'generate':
        job_cmd = argv[:2]
        argv_parser = argv[2:]
        prog += ' generate'
    else:
        job_cmd = argv[:1]
        argv_parser = argv[1:]
    # Parse arguments
    parser = argparse.ArgumentParser(prog=prog, description='Generate pipeline config.')
    parser.add_argument('-p', '--project', dest='project', action='store', help='Project')
    parser.add_argument('-r', '--replicate', dest='replicate', action='store_true', help='List replicates instead of runs')
    parser.add_argument('-n', '--infos', dest='infos', action='store_true', help='Include sample infos from database')
    parser.add_argument('-o', '--path_output', dest='path_output', action='store', default='-', help='Path to output (\'-\' for stdout)')
    parser.add_argument('--path_config', dest='path_config', action='store', help='Path to config')
    parser.add_argument('--http_url', '--labxdb_http_url', dest='labxdb_http_url', action='store', help='Database HTTP URL')
    parser.add_argument('--http_login', '--labxdb_http_login', dest='labxdb_http_login', action='store', help='Database HTTP login')
    parser.add_argument('--http_password', '--labxdb_http_password', dest='labxdb_http_password', action='store', help='Database HTTP password')
    parser.add_argument('--http_path', '--labxdb_http_path', dest='labxdb_http_path', action='store', help='Database HTTP path')
    parser.add_argument('--http_db', '--labxdb_http_db', dest='labxdb_http_db', action='store', help='Database HTTP DB')
    args = parser.parse_args(argv_parser)

    # Get config (JSON single file or all files in path_config)
    config = {}
    paths = []
    if args.path_config is None:
        if 'HTS_CONFIG_PATH' in os.environ:
            paths.append(os.environ['HTS_CONFIG_PATH'])
        elif 'XDG_CONFIG_HOME' in os.environ:
            paths.append(os.path.join(os.environ['XDG_CONFIG_HOME'], 'hts'))
    else:
        paths.append(args.path_config)
    for path in paths:
        if os.path.isdir(path):
            for f in sorted(os.listdir(path)):
                if f.endswith('.json'):
                    config = {**config, **json.load(open(os.path.join(path, f)))}
        elif os.path.isfile(path):
            config = {**config, **json.load(open(path))}

    # Input local config from args
    vargs = vars(args)
    for a, v in vargs.items():
        if v is not None and (a not in config or v != parser.get_default(a)):
            config[a] = v

    # Init. DBLink
    if 'labxdb_http_path' not in config and 'labxdb_http_db' not in config:
        if 'labxdb_http_path_seq' in config:
            config['labxdb_http_path'] = config['labxdb_http_path_seq']
        else:
            config['labxdb_http_db'] = 'seq'
    dbl = labxdb.DBLink(config.get('labxdb_http_url'), config.get('labxdb_http_login'), config.get('labxdb_http_password'), config.get('labxdb_http_path'), config.get('labxdb_http_db'))

    # Generate new pipeline from project ID
    if 'project' in config:
        # Empty project
        empty_project = {'name': '', 'path_output': '', 'logging_level': 'info'}

        # Add refs
        projects = dbl.post('tree', {'search_criterion':['0 project_ref EQUAL '+config['project']], 'search_gate':'AND', 'limit':'ALL'})
        if args.replicate:
            refs = sorted([replicate['replicate_ref'] for project in projects for sample in project['children'] for replicate in sample['children']])
            empty_project['replicate_refs'] = refs
        else:
            refs = sorted([run['run_ref'] for project in projects for sample in project['children'] for replicate in sample['children'] for run in replicate['children']])
            empty_project['run_refs'] = refs

        # Add sample infos
        if args.infos:
            empty_project['ref_info_source'] = ['json']

            # Prepare sample infos
            ref_infos = []
            for project in projects:
                for sample in project['children']:
                    # Get adapter sequence
                    if sample['adapter_5p'] is not None and sample['adapter_5p'] in config['adaptors']:
                        adaptor_5p = config['adaptors'][sample['adapter_5p']]
                    else:
                        adaptor_5p = None
                    if sample['adapter_3p'] is not None and sample['adapter_3p'] in config['adaptors']:
                        adaptor_3p = config['adaptors'][sample['adapter_3p']]
                    else:
                        adaptor_3p = None
                    for replicate in sample['children']:
                        for run in replicate['children']:
                            paired = run['paired']
                            directional = run['directional']
                            r1_strand = run['r1_strand']
                            quality_scores = run['quality_scores']
                            if 'run_refs' in empty_project and run['run_ref'] in empty_project['run_refs']:
                                d = {
                                    'label_short': replicate['label_short'],
                                    'label_long': replicate['label_long'],
                                    'paired': paired,
                                    'directional': directional,
                                    'r1_strand': r1_strand,
                                    'quality_scores': quality_scores,
                                }
                                if adaptor_5p is not None:
                                    d['adaptor_5p'] = adaptor_5p
                                if adaptor_3p is not None:
                                    d['adaptor_3p'] = adaptor_3p
                                ref_infos.append((run['run_ref'], d))
                        if 'replicate_refs' in empty_project and run['replicate_ref'] in empty_project['replicate_refs']:
                            d = {
                                'label_short': replicate['label_short'],
                                'label_long': replicate['label_long'],
                                'paired': paired,
                                'directional': directional,
                                'r1_strand': r1_strand,
                                'quality_scores': quality_scores,
                            }
                            if adaptor_5p is not None:
                                d['adaptor_5p'] = adaptor_5p
                            if adaptor_3p is not None:
                                d['adaptor_3p'] = adaptor_3p
                            ref_infos.append((replicate['replicate_ref'], d))

            # Add sample infos to project
            ref_infos.sort(key=lambda x: x[0])
            empty_project['ref_infos'] = dict(ref_infos)

        # Add analysis
        empty_project['analysis'] = []

        # Output
        out = json.dumps(empty_project, indent=4, separators=(',', ': '))
        if args.path_output == '-':
            print(out)
        else:
            with open(args.path_output, 'wt') as f:
                f.write(out)

if __name__ == '__main__':
    sys.exit(main())

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