using FileIO using Printf include("julia2ims.jl") """ 20201216: Package Spinning disk tif stack to imaris """ src_dir = "/datahub/rawdata/tandeng/mRNA_imaging/mRNA_confocal_again/20201215/sCMOS" out_dir = "/datahub/rawdata/tandeng/mRNA_imaging/mRNA_confocal_again/20201215" #src_dir = ARGS[1] t_len = 42 # 79-120 time point z_len = 20 pos_len = 40 buffer = zeros(N0f16, 1900, 1300, z_len*t_len*pos_len) img_num = 866 raw = load(File(format"TIFF", "$src_dir/HE7-1NLS-uw-PWM_1_MMStack_72.ome.tif")) buffer[:, :, 1:818] = raw[:, :, 49:end] # push 364 slice Threads.@threads for index in 73:109 # 18 file here, remain 412 slice println(index) filename = "HE7-1NLS-uw-PWM_1_MMStack_$index.ome.tif" raw = load(File(format"TIFF", "$src_dir/$filename")) GC.gc() id = (index-73)*img_num + 1 + 818 buffer[:, :, id:id+img_num-1] = raw end raw = load(File(format"TIFF", "$src_dir/HE7-1NLS-uw-PWM_1_MMStack_110.ome.tif")) buffer[:, :, end-740+1:end] = raw[:, :, :] # push 48 slice buffer = reshape(buffer, (1900, 1300, z_len, t_len*pos_len)) Threads.@threads for pos in 1:pos_len imsname = @sprintf("%s/pos%02d-4.tiff", out_dir, pos) #save2ims(reinterpret(UInt16, buffer[:, :, :, :, pos]), # imsname, dx=0.108, compression=1) save(imsname, reshape(buffer[:, :, :, pos:40:end], (1900, 1300, z_len*t_len))) end