ptg781-ef1alpha-3xflag-halo-3xnls-p2a-t2a-snapf-3xnls.gb
LOCUS pTG781_EF1alpha-3xFlag- 8886 bp ds-DNA circular 11-FEB-2021
DEFINITION .
KEYWORDS "creator:Gina Dailey - Darzacq/Tjian Lab" "marker:AmpR, PuroR"
FEATURES Location/Qualifiers
primer complement(108..126)
/label="oTG1552_pBS_diffusion_substrate_300bp"
/note="sequence: ctctagcttcccggcaaca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS complement(650..718)
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter complement(719..823)
/label="AmpR promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(802..823)
/label="oTG1179_FRT_AmpR_5’"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCcgcggaacccctatttgtttat"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(821..839)
/label="oTG1553_pBS_diffusion_substrate_1kb"
/note="sequence: ttttcggggaaatgtgcgc"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
rep_origin complement(849..1303)
/label="f1 ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 1441..1460
/label="oTG1336_M13F_extended"
/note="sequence: GTTGTAAAACGACGGCCAGT"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind 1443..1460
/label="M13-fwd"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 1444..1460
/label="M13 fwd"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
misc_feature 1590..1602
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 1637..1652
/label="IR (inverted repeat) truncated?"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
promoter 2071..2570
/label="PGK promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS 2592..3191
/label="PuroR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
polyA_signal 3224..3448
/label="bGH poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
CDS 4012..4014
/label="ATG"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 4012..5823
/label="Translation 4012-5823"
CDS 4015..4080
/label="3xFLAG"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 4089..4114
/label="oTG945_60xHalo_F"
/note="sequence: CCGGCTCCGGCAGCGGTAGCATGGCAGAAATCGGTACTGGCTTTC"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 4090..4110
/label="oTG778_5'Halo"
/note="sequence: ATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 4090..4110
/label="oTG898_H10_Halo_F"
/note="sequence: TTCTCACCATCACCATCACCACCACCACCACCATATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 4090..4110
/label="oTG1573_GSGSKCK_Halo_F"
/note="sequence: GGTAGTGGTAGTAAGTGTAAAATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
CDS 4090..4980
/label="HaloTag®"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 4093..4113
/label="oTG1144_inVitro_60xHalo_F"
/note="sequence: GGCTCCGGCAGCGGTAGCGCAGAAATCGGTACTGGCTTT"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer 4096..4118
/label="oTG995_Halo_linker_F"
/note="sequence: GATCTGGAGGTTCAGGAGGCAGCGGTGGAAGTGAAATCGGTACTGGCTTTCCATT"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer 4096..4118
/label="oTG1280_60mer_linker_Halo_F"
/note="sequence: GGAGTGGTAGCGGCAGTATTAAGgaaatcggtactggctttccatt"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(4101..4120)
/label="oTG1172_Halo_int_R2"
/note="sequence: CGAATGGAAAGCCAGTACCG"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(4106..4125)
/label="oTG1214_Halo_int_R_75"
/note="sequence: GGGGTCGAATGGAAAGCCAG"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(4129..4148)
/label="oTG1096_Halo_int_R"
/note="sequence: TCGCCCAGGACTTCCACATA"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(4150..4169)
/label="oTG1173_Halo_int_R3"
/note="sequence: ACATCGACGTAGTGCATGCG"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 4311..4332
/label="oTG1513_Halo_qPCR_F1"
/note="sequence: ACCAGACCTGGGTTATTTCTTC"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer 4451..4470
/label="oTG1208_Halo_int_F"
/note="sequence: AGCGCGTCAAAGGTATTGCA"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(4471..4490)
/label="oTG1514_Halo_qPCR_R1"
/note="sequence: GGGCGGATGAACTCCATAAA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(4629..4650)
/label="oTG1207_Halo_int_R"
/note="sequence: GTCCATCTCGACTTCAGTCAGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 4636..4657
/label="oTG1515_Halo_qPCR_F2"
/note="sequence: GAAGTCGAGATGGACCATTACC"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer complement(4758..4777)
/label="oTG1516_Halo_qPCR_R2"
/note="sequence: GCAGCCAGTCCATGTATTCT"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 4871..4890
/label="oTG1095_Halo_int_F"
/note="sequence: ACTGCAAGGCTGTGGACATC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 4898..4920
/label="oTG979_3'_Halo_seq"
/note="sequence: GTCTGAATCTGCTGCAAGAAGAC"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer complement(4948..4965)
/label="oTG1312_Halo_tubUTR_R"
/note="sequence: cgcgacgcttagtgtgccgcTTACGTCGACAGCCAGCGCGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(4960..4979)
/label="oTG1261_ParB2_Halo_stop_R"
/note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(4960..4979)
/label="oTG1261_ParB2_Halo_stop_R"
/note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(4961..4980)
/label="oTG793_3'Halo_R"
/note="sequence: GCCGGAAATCTCGAGCGTCG"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer complement(4961..4980)
/label="oTG779_Halo-stop_pETSUMO"
/note="sequence: GCTCCAATTGGGTACCCATGGAGCCGGAAATCTCGAGCGTCG"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
misc_feature 5044..5112
/label="3xNLS"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 5119..5136
/label="oTG1684_P2A-T2A_KpnI_F"
/note="sequence: atttccggcggtagtggtaccGGCAGCGGCGCCACAAAC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 5119..5184
/label="P2A"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 5203..5265
/label="T2A"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
primer 5278..5300
/label="oTG1562_FKBP-SNAPf_F2"
/note="sequence: atggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
CDS 5278..5823
/label="SNAPf"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
gene 5278..5823
/label="SNAP26f"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 5278..5823
/label="Translation 5278-5823"
source 5278..5823
/label="source"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(5804..5823)
/label="oTG1563_FKBP-SNAPf_R2"
/note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
CDS 5824..5835
/label="HaloTag®"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
CDS 5824..5964
/label="Translation 5824-5964"
primer 5839..5859
/label="oTG1415_7-SspB-p65-Fwd-OptoFly-GA"
/note="sequence: ggagatggcgctggactgatc"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
CDS 5839..5862
/label="GDGAGLIN linker"
/ApEinfo_revcolor=#cd4489
/ApEinfo_fwdcolor=#cd4489
misc_feature 5865..5865
/label="add_after"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 5875..5895
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 5899..5919
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 5923..5943
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
primer 5942..5966
/label="oTG967_pHalo-Stop_F"
/note="sequence: TAGGATCCACCGGATCTAGATAACT"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
misc_feature 5958..5968
/label="stop codons"
/ApEinfo_revcolor=#ff0000
/ApEinfo_fwdcolor=#ff0000
misc_feature 6045..6045
/label="add_before"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 6045..6062
/label="MCS"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
polyA_signal 6178..6299
/label="SV40 poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(6271..6299)
/label="oTG885_SV40_pA_F"
/note="sequence: TAAGATACATTGATGAGTTTGGACAAACC"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature complement(7059..7077)
/label="IR (inverted repeat)"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
misc_feature complement(7081..7093)
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind complement(7584..7600)
/label="M13 rev"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
protein_bind 7608..7624
/label="lac operator"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
promoter complement(7632..7638)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(7639..7656)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(7650..7671)
/label="oTG980_Vector-Fwd-GA"
/note="sequence: ccggatgacagtgacgaggagaactgaACCCCAGGCTTTacacTTTATG"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
promoter complement(7657..7662)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
protein_bind 7677..7698
/label="CAP binding site"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
primer 7729..7749
/label="oTG1156_mCherry-LacI-Rev"
/note="sequence: AAGCTTCCATTCGGGAAACCTGTCGtgCCAG"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 7749..7769
/label="oTG1089_Rev-LacI-NLS-NOSTOP-GA"
/note="sequence: AACCTTCCTCTTCTTCTTAGGAGGCTGCATTAATGAATCGGCCA"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 7749..7770
/label="oTG1084_Rev-LacI-GA"
/note="sequence: TCAAACCTTCCTCTtcTTcTTaGgagGCTGCATTAATGAATCGGCCAA"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 7806..7823
/label="oTG775_pET_BbvCI_insert_seq"
/note="sequence: TTCCGCTTCCTCGCTCAC"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
rep_origin complement(7986..8573)
/label="ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 8627..8650
/label="oTG1180_AmpR_seq"
/note="sequence: ACGTTAAGGGATTTTGGTCATGAG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(8713..8743)
/label="oTG1177_FRT_AmpR_3’flanking"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer complement(8713..8743)
/label="oTG1554_pBS_diffusion_substrate_3kb"
/note="sequence: ctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 8714..8743
/label="oTG1182_E75_AmpR_F"
/note="sequence: gactaactgccactcgcagcgcgcagatcgAAGTATATATGAGTAAACTTGGTCTGACAG"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer 8743..8767
/label="oTG1549_pBS_849_adapter"
/note="sequence: GCTGTGTCTTATTACACACAGTCGttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
CDS complement(join(8744..8886,1..649))
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 8744..8767
/label="oTG1176_FRT_AmpR_3’"
/note="sequence: CTATTCTCTAGAAAGTATAGGAACTTCttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(8790..8812)
/label="oTG1550_pBS_diffusion_substrate_100bp"
/note="sequence: caggcaactatggatgaacgaaa"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC
61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG
121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA
181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA
241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA
301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA
361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA
421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG
481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG
541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG
601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG
661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC
721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT
901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA
961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA
1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA
1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG
1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA
1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG
1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG
1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC
1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG
1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG
1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG
1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT
1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA
1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT
1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT
1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT
1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA
1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC
2041 TGGGGGGATA CGGGGAAAAG GCGAACGCGT gggtagggga ggcgcttttc ccaaggcagt
2101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
2161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
2221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
2281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
2341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
2401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
2461 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
2521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
2581 caAGCTTCAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC
2641 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG
2701 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG
2761 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA
2821 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG
2881 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC
2941 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA
3001 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC
3061 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA
3121 CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC
3181 CCGGTGCCTG AGCGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AACGCGATCG CTCCGGTGCC
3481 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC
3541 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC
3601 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG
3661 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC
3721 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC
3781 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA
3841 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC
3901 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT
3961 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCCAC CATGGACTAC
4021 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGACAAG
4081 CTTGGCTCCA TGGCAGAAAT CGGTACTGGC TTTCCATTCG ACCCCCATTA TGTGGAAGTC
4141 CTGGGCGAGC GCATGCACTA CGTCGATGTT GGTCCGCGCG ATGGCACCCC TGTGCTGTTC
4201 CTGCACGGTA ACCCGACCTC CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG
4261 ACCCATCGCT GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA ACCAGACCTG
4321 GGTTATTTCT TCGACGACCA CGTCCGCTTC ATGGATGCCT TCATCGAAGC CCTGGGTCTG
4381 GAAGAGGTCG TCCTGGTCAT TCACGACTGG GGCTCCGCTC TGGGTTTCCA CTGGGCCAAG
4441 CGCAATCCAG AGCGCGTCAA AGGTATTGCA TTTATGGAGT TCATCCGCCC TATCCCGACC
4501 TGGGACGAAT GGCCAGAATT TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC
4561 GGCCGCAAGC TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC GATGGGTGTC
4621 GTCCGCCCGC TGACTGAAGT CGAGATGGAC CATTACCGCG AGCCGTTCCT GAATCCTGTT
4681 GACCGCGAGC CACTGTGGCG CTTCCCAAAC GAGCTGCCAA TCGCCGGTGA GCCAGCGAAC
4741 ATCGTCGCGC TGGTCGAAGA ATACATGGAC TGGCTGCACC AGTCCCCTGT CCCGAAGCTG
4801 CTGTTCTGGG GCACCCCAGG CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA
4861 AGCCTGCCTA ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT GCAAGAAGAC
4921 AACCCGGACC TGATCGGCAG CGAGATCGCG CGCTGGCTGT CGACGCTCGA GATTTCCGGC
4981 ggtagtggta ccTCAGGAAG TGGAGATGGC GCCGGATTGA TCAATGGACG GGCAGATgct
5041 agcCCGAAGA AAAAGCGGAA GGTCGATCCC AAAAAGAAGA GGAAAGTGGA CCCAAAGAAG
5101 AAACGGAAGG TAcatatggg cagcggcgcc acaaacttct ctctgctaaa gcaagcaggt
5161 gatgttgaag aaaaccccgg gcctACTAGT GCTGGAAAGC TTggctccgg cgagggcagg
5221 ggaagtcttc taacatgcgg ggacgtggag gaaaatcccg gcccaggtac cggttctatg
5281 gacaaagact gcgaaatgaa gcgcaccacc ctggatagcc ctctgggcaa gctggaactg
5341 tctgggtgcg aacagggcct gcaccgtatc atcttcctgg gcaaaggaac atctgccgcc
5401 gacgccgtgg aagtgcctgc cccagccgcc gtgctgggcg gaccagagcc actgatgcag
5461 gccaccgcct ggctcaacgc ctactttcac cagcctgagg ccatcgagga gttccctgtg
5521 ccagccctgc accacccagt gttccagcag gagagcttta cccgccaggt gctgtggaaa
5581 ctgctgaaag tggtgaagtt cggagaggtc atcagctaca gccacctggc cgccctggcc
5641 ggcaatcccg ccgccaccgc cgccgtgaaa accgccctga gcggaaatcc cgtgcccatt
5701 ctgatcccct gccaccgggt ggtgcagggc gacctggacg tggggggcta cgagggcggg
5761 ctcgccgtga aagagtggct gctggcccac gagggccaca gactgggcaa gcctgggctg
5821 ggtGAGATTT CCGGCagcgg agatggcgct ggactgatca acggcCGAGC TGATCCAAAA
5881 AAGAAGAGAA AGGTAGATCC AAAAAAGAAG AGAAAGGTAG ATCCAAAAAA GAAGAGAAAG
5941 GTAGGATCCA CCGGATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGAGGTT
6001 TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAACGCGGC CGCtggGTCG
6061 ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG
6121 CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCG TTAACTAAAC
6181 TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT
6241 AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT
6301 CATGTCTGGA ATTGACTCAA ATGATGTCAA TTAGTCTATC AGAAGCTCAT CTGGTCTCCC
6361 TTCCGGGGGA CAAGACATCC CTGTTTAATA TTTAAACAGC AGTGTTCCCA AACTGGGTTC
6421 TTATATCCCT TGCTCTGGTC AACCAGGTTG CAGGGTTTCC TGTCCTCACA GGAACGAAGT
6481 CCCTAAAGAA ACAGTGGCAG CCAGGTTTAG CCCCGGAATT GACTGGATTC CTTTTTTAGG
6541 GCCCATTGGT ATGGCTTTTT CCCCGTATCC CCCCAGGTGT CTGCAGGCTC AAAGAGCAGC
6601 GAGAAGCGTT CAGAGGAAAG CGATCCCGTG CCACCTTCCC CGTGCCCGGG CTGTCCCCGC
6661 ACGCTGCCGG CTCGGGGATG CGGGGGGAGC GCCGGACCGG AGCGGAGCCC CGGGCGGCTC
6721 GCTGCTGCCC CCTAGCGGGG GAGGGACGTA ATTACATCCC TGGGGGCTTT GGGGGGGGGC
6781 TGTCCCTGAT ATCTATAACA AGAAATATAT ATATAATAAG TTATCACGTA AGTAGAACAT
6841 GAAATAACAA TATAATTATC GTATGAGTTA ATCTTAAAGT CACGTAAAAG ATAATCATGC
6901 GTCATTTTGA CTCACGCGGT CGTTATAGTT CAAAATCAGT GACACTTACC GCATTGACAA
6961 GCACGCCTCA CGGGAGCTCC AAGCGGCGAC TGAGATGTCC TAAATGCACA GCGACGGATT
7021 CGCGCTATTT AGAAAGAGAG AGCAATATTT CAAGAATGCA TGCGTCAATT TTACGCAGAC
7081 TATCTTTCTA GGGTTAATCT AGCTGCATCA GGATCATATC GTCGGGTCTT TTTTCCGGCT
7141 CAGTCATCGC CCAAGCTGGC GCTATCTGGG CATCGGGGAG GAAGAAGCCC GTGCCTTTTC
7201 CCGCGAGGTT GAAGCGGCAT GGAAAGAGTT TGCCGAGGAT GACTGCTGCT GCATTGACGT
7261 TGAGCGAAAA CGCACGTTTA CCATGATGAT TCGGGAAGGT GTGGCCATGC ACGCCTTTAA
7321 CGGTGAACTG TTCGTTCAGG CCACCTGGGA TACCAGTTCG TCGCGGCTTT TCCGGACACA
7381 GTTCCGGATG GTCAGCCCGA AGCGCATCAG CAACCCGAAC AATACCGGCG ACAGCCGGAA
7441 CTGCCGTGCC GGTGTGCAGA TTAATGACAG CGGTGCGGCG CTGGGATATT ACGTCAGCGA
7501 GGACGGGTAT CCTGGCTGGA TGCCGCAGAA ATGGACATGG ATACCCCGTG AGTTACCCGG
7561 CGGGCGCGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC
7621 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA
7681 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG
7741 TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG
7801 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG
7861 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA
7921 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
7981 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG
8041 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
8101 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
8161 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT
8221 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
8281 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC
8341 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
8401 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA
8461 GTTACCTTCG GAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
8521 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
8581 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT
8641 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT
8701 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA
8761 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG
8821 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
8881 CGCGAG
//