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  • README.txt
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To reference or cite the objects present in the Software Heritage archive, permalinks based on SoftWare Hash IDentifiers (SWHIDs) must be used.
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(requires biblatex-software package)
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(requires biblatex-software package)
Generating citation ...
README.txt
Script to do PCA and DEseq2 analysis
=============================================================================================

This script performs PCA and DEseq2 analysis, output differentially expressed genes, and generate MA plots and PCA plots. 

Related to Figure 5B, as well as Figure 5-figure supplements
 

Directory Structure:
==========================

------------------------------+
Input   |
------------------------------+
1. The Count supermatrix .csv file generated using the '2_getCombinedCountMatrix_220805_codechk.pl' script
2. Experimental design .csv file that contains library name, sex, feeding, batch, and condition (a complex variable with all the info). 

------------------------------+
Plots   |
------------------------------+
This is where the PCA plots and MA plots will be stored.


-----------------+
Output          |
-----------------+
This is where all the DEseq2 comparisons and normalized counts will be stored. 

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