https://gitlab.com/tgwgraham/papa_paper_plasmids
Tip revision: 984803582c0023a9fe975ec6d41be3d723c2737b authored by Thomas Graham on 08 July 2022, 01:06:31 UTC
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Tip revision: 9848035
ptg824-ef1alpha-snapf-3xnls-t2a-p2a-h2b-halo-snapf.gb
LOCUS pTG824_EF1alpha-SNAPf-3 9768 bp ds-DNA circular 01-JUL-2021
DEFINITION .
KEYWORDS "creator:Gina Dailey - Darzacq/Tjian Lab" "marker:AmpR, PuroR"
FEATURES Location/Qualifiers
primer complement(108..126)
/label="oTG1552_pBS_diffusion_substrate_300bp"
/note="sequence: ctctagcttcccggcaaca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS complement(650..718)
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter complement(719..823)
/label="AmpR promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(802..823)
/label="oTG1179_FRT_AmpR_5’"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCcgcggaacccctatttgtttat"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(821..839)
/label="oTG1553_pBS_diffusion_substrate_1kb"
/note="sequence: ttttcggggaaatgtgcgc"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
rep_origin complement(849..1303)
/label="f1 ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 1441..1460
/label="oTG1336_M13F_extended"
/note="sequence: GTTGTAAAACGACGGCCAGT"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind 1443..1460
/label="M13-fwd"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 1444..1460
/label="M13 fwd"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
misc_feature 1590..1602
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 1637..1652
/label="IR (inverted repeat) truncated?"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
promoter 2071..2570
/label="PGK promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS 2592..3191
/label="PuroR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
polyA_signal 3224..3448
/label="bGH poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 3991..4010
/label="oTG1576_3xFlag-Halo_upstream_F"
/note="sequence: CCGGCGCCTACTCTAGCCAC"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 3991..4034
/label="oTG1580_Kozak_SNAPf_F"
/note="sequence: CCGGCGCCTACTCTAGCCACCatggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
misc_feature 4012..4026
/label="Gibson overhang"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 4012..4033
/label="oTG1577_Halo_linker_SNAPf_F"
/note="sequence: CGACGCTCGAGATTTCCGGCggtagtggtaccggttctATGGACAAAGACTGCGAAATGA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 4012..4034
/label="oTG1580_Kozak_SNAPf_F"
/note="sequence: CCGGCGCCTACTCTAGCCACCatggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 4012..4034
/label="oTG1562_FKBP-SNAPf_F2"
/note="sequence: atggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
gene 4012..4557
/label="SNAP26f"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 4012..4557
/label="SNAPf"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
source 4012..4557
/label="source"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS 4012..6825
/label="Translation 4012-6825"
primer complement(4099..4119)
/label="oTG1722_SNAPf_v2_int_R"
/note="sequence: tttgcccaggaagatgatacg"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4538..4557)
/label="oTG1731_SNAPf_stop_BamHI_R"
/note="sequence: TCAGTTATCTAGATCCGGTGGATCCTTAacccagcccaggcttgccca"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4538..4557)
/label="oTG1581_SNAPf_3’_R"
/note="sequence: acccagcccaggcttgccca"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(4538..4557)
/label="oTG1563_FKBP-SNAPf_R2"
/note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(4538..4558)
/label="oTG1578_SNAPf_noNLS_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCacccagcccaggcttgccca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(4538..4577)
/label="oTG1563_FKBP-SNAPf_R2"
/note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
CDS 4558..4569
/label="HaloTag®"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 4573..4593
/label="oTG1415_7-SspB-p65-Fwd-OptoFly-GA"
/note="sequence: ggagatggcgctggactgatc"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
CDS 4573..4596
/label="GDGAGLIN linker"
/ApEinfo_revcolor=#cd4489
/ApEinfo_fwdcolor=#cd4489
misc_feature 4599..4599
/label="add_after"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 4609..4629
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 4633..4653
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 4657..4677
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
primer complement(4669..4689)
/label="oTG1724_SNAPf-3xNLS_R"
/note="sequence: tccggtggatcctacctttct"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4669..4707
/label="oTG1725_SNAPf-3xNLS_P2A_F"
/note="sequence: AGAAAGGTAGGATCCACCGGAcatatgggcagcggcgcc"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4696..4713
/label="oTG1684_P2A-T2A_KpnI_F"
/note="sequence: atttccggcggtagtggtaccGGCAGCGGCGCCACAAAC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 4696..4761
/label="P2A"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 4780..4842
/label="T2A"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
primer complement(4825..4848)
/label="oTG1685_P2A-T2A_KpnI_R"
/note="sequence: tttgtccatagaaccggtaccTGGGCCGGGATTTTCCTC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4828..4866
/label="oTG1628_3xF-HaloSNAP-3xN-TP2A-H2B-SNAP_F"
/note="sequence: gaaaatcccggcccaGgtaccATGCCTGAGCCTGCGAAG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4829..4866
/label="oTG1727_T2A-KpnI-H2B_F"
/note="sequence: AAAATCCCGGCCCAGGTACCatgcctgagcctgcgaag"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4831..4848)
/label="oTG1726_T2A-KpnI_R"
/note="sequence: ggtacctgggccgggatt"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4846..4866
/label="oTG1626_H2B-Halo-SNAP-TP2A-SNAP_F"
/note="sequence: cggcgcctactctagccaccATGCCTGAGCCTGCGAAG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
CDS 4849..5226
/label="H2be"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
primer complement(5220..5238)
/label="oTG1629_3xF-HaloSNAP-3xN-TP2A-H2B-SNAP_R"
/note="sequence: tccacttccgccaccggtaccGGATCCTCCGGACTTGGAG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5220..5264)
/label="oTG1627_H2B-Halo-SNAP-TP2A-SNAP_R"
/note="sequence: aagccagtaccgatttctgccatggaGGATCCTCCGGACTTGGAG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5240..5262
/label="oTG1730_T2A_Halo_F"
/note="sequence: AAAATCCCGGCCCAGGTACCatggcagaaatcggtactggc"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5241..5266
/label="oTG945_60xHalo_F"
/note="sequence: CCGGCTCCGGCAGCGGTAGCATGGCAGAAATCGGTACTGGCTTTC"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 5242..5262
/label="oTG898_H10_Halo_F"
/note="sequence: TTCTCACCATCACCATCACCACCACCACCACCATATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 5242..5262
/label="oTG778_5'Halo"
/note="sequence: ATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 5242..5262
/label="oTG1582_SNAPf_linker_Halo_F"
/note="sequence: TGGGCAAGCCTGGGCTGGGTggtagtggtaccggttctATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 5242..5262
/label="oTG1573_GSGSKCK_Halo_F"
/note="sequence: GGTAGTGGTAGTAAGTGTAAAATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
CDS 5242..6132
/label="HaloTag®"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 5245..5265
/label="oTG1144_inVitro_60xHalo_F"
/note="sequence: GGCTCCGGCAGCGGTAGCGCAGAAATCGGTACTGGCTTT"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer 5248..5270
/label="oTG995_Halo_linker_F"
/note="sequence: GATCTGGAGGTTCAGGAGGCAGCGGTGGAAGTGAAATCGGTACTGGCTTTCCATT"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer 5248..5270
/label="oTG1280_60mer_linker_Halo_F"
/note="sequence: GGAGTGGTAGCGGCAGTATTAAGgaaatcggtactggctttccatt"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(5253..5272)
/label="oTG1172_Halo_int_R2"
/note="sequence: CGAATGGAAAGCCAGTACCG"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(5258..5277)
/label="oTG1214_Halo_int_R_75"
/note="sequence: GGGGTCGAATGGAAAGCCAG"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(5281..5300)
/label="oTG1096_Halo_int_R"
/note="sequence: TCGCCCAGGACTTCCACATA"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(5302..5321)
/label="oTG1173_Halo_int_R3"
/note="sequence: ACATCGACGTAGTGCATGCG"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 5463..5484
/label="oTG1513_Halo_qPCR_F1"
/note="sequence: ACCAGACCTGGGTTATTTCTTC"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer 5603..5622
/label="oTG1208_Halo_int_F"
/note="sequence: AGCGCGTCAAAGGTATTGCA"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(5623..5642)
/label="oTG1514_Halo_qPCR_R1"
/note="sequence: GGGCGGATGAACTCCATAAA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(5717..5739)
/label="oTG1674_Halo_V167C_R"
/note="sequence: GTTCTGATCGATGATCAGCTTGC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5717..5739)
/label="oTG1691_Halo_V167C_R"
/note="sequence: GTTCTGATCGATGATCAGCTTGC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5726..5751)
/label="oTG1693_Halo_G171C_R"
/note="sequence: CTCGATAAAAACGTTCTGATCGATGA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5726..5751)
/label="oTG1676_Halo_G171C_R"
/note="sequence: CTCGATAAAAACGTTCTGATCGATGA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5742..5762
/label="oTG1673_Halo_V167C_F"
/note="sequence: tgtTTTATCGAGGGTACGCTGCC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5742..5762
/label="oTG1690_Halo_V167C_F"
/note="sequence: tgtTTTATCGAGGGTACGCTGCC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5744..5763)
/label="oTG1695_Halo_M175C_R"
/note="sequence: CGGCAGCGTACCCTCGATAA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5744..5763)
/label="oTG1678_Halo_M175C_R"
/note="sequence: CGGCAGCGTACCCTCGATAA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5753..5772
/label="oTG1675_Halo_G171C_F"
/note="sequence: tgtACGCTGCCGATGGGTGTC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5753..5772
/label="oTG1692_Halo_G171C_F"
/note="sequence: tgtACGCTGCCGATGGGTGTC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5767..5784
/label="oTG1677_Halo_M175C_F"
/note="sequence: tgtGGTGTCGTCCGCCCGCTG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5767..5784
/label="oTG1694_Halo_M175C_F"
/note="sequence: tgtGGTGTCGTCCGCCCGCTG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5781..5802)
/label="oTG1207_Halo_int_R"
/note="sequence: GTCCATCTCGACTTCAGTCAGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 5788..5809
/label="oTG1515_Halo_qPCR_F2"
/note="sequence: GAAGTCGAGATGGACCATTACC"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer complement(5910..5929)
/label="oTG1516_Halo_qPCR_R2"
/note="sequence: GCAGCCAGTCCATGTATTCT"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 6023..6042
/label="oTG1095_Halo_int_F"
/note="sequence: ACTGCAAGGCTGTGGACATC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 6050..6072
/label="oTG979_3'_Halo_seq"
/note="sequence: GTCTGAATCTGCTGCAAGAAGAC"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer complement(6100..6117)
/label="oTG1312_Halo_tubUTR_R"
/note="sequence: cgcgacgcttagtgtgccgcTTACGTCGACAGCCAGCGCGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(6112..6131)
/label="oTG1261_ParB2_Halo_stop_R"
/note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(6112..6131)
/label="oTG1261_ParB2_Halo_stop_R"
/note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(6113..6132)
/label="oTG779_Halo-stop_pETSUMO"
/note="sequence: GCTCCAATTGGGTACCCATGGAGCCGGAAATCTCGAGCGTCG"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer complement(6113..6132)
/label="oTG793_3'Halo_R"
/note="sequence: GCCGGAAATCTCGAGCGTCG"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer complement(6113..6134)
/label="oTG1583_Halo_noNLS_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCgccggaaatctcgagcgtcg"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 6124..6143
/label="Gibson overhang"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 6124..6143
/label="oTG1618_SNAP-3xNLS_gBlock_F"
/note="sequence: ATTTCCGGCggtagtggtac"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 6151..6175
/label="oTG1729_Kozak_SNAPfv2_F"
/note="sequence: CCGGCGCCTACTCTAGCCACCatggataaagactgtgagatgaagc"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 6151..6696
/label="SNAPf"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 6702..6721
/label="oTG1683_3xNLS-P2A-T2A_KpnI_F"
/note="sequence: atttccggcggtagtggtaccTCAGGAAGTGGAGATGGCG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 6754..6822
/label="3xNLS"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(6800..6825)
/label="oTG1728_3xNLS_stop_BamHI_R"
/note="sequence: TCAGTTATCTAGATCCGGTGGATCCttataccttccgtttcttctttgggt"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(6800..6850)
/label="oTG1728_3xNLS_stop_BamHI_R"
/note="sequence: TCAGTTATCTAGATCCGGTGGATCCttataccttccgtttcttctttgggt"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 6824..6848
/label="oTG967_pHalo-Stop_F"
/note="sequence: TAGGATCCACCGGATCTAGATAACT"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 6825..6848
/label="oTG967_pHalo-Stop_F"
/note="sequence: TAGGATCCACCGGATCTAGATAACT"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 6826..6848
/label="oTG967_pHalo-Stop_F"
/note="sequence: TAGGATCCACCGGATCTAGATAACT"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(6831..6859)
/label="oTG1579_3xNLS_downstream_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTG"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 6840..6850
/label="stop codons"
/ApEinfo_revcolor=#ff0000
/ApEinfo_fwdcolor=#ff0000
misc_feature 6927..6927
/label="add_before"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 6927..6944
/label="MCS"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
polyA_signal 7060..7181
/label="SV40 poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(7153..7181)
/label="oTG885_SV40_pA_F"
/note="sequence: TAAGATACATTGATGAGTTTGGACAAACC"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature complement(7941..7959)
/label="IR (inverted repeat)"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
misc_feature complement(7963..7975)
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind complement(8466..8482)
/label="M13 rev"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
protein_bind 8490..8506
/label="lac operator"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
promoter complement(8514..8520)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(8521..8538)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(8532..8553)
/label="oTG980_Vector-Fwd-GA"
/note="sequence: ccggatgacagtgacgaggagaactgaACCCCAGGCTTTacacTTTATG"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
promoter complement(8539..8544)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
protein_bind 8559..8580
/label="CAP binding site"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
primer 8611..8631
/label="oTG1156_mCherry-LacI-Rev"
/note="sequence: AAGCTTCCATTCGGGAAACCTGTCGtgCCAG"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 8631..8651
/label="oTG1089_Rev-LacI-NLS-NOSTOP-GA"
/note="sequence: AACCTTCCTCTTCTTCTTAGGAGGCTGCATTAATGAATCGGCCA"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 8631..8652
/label="oTG1084_Rev-LacI-GA"
/note="sequence: TCAAACCTTCCTCTtcTTcTTaGgagGCTGCATTAATGAATCGGCCAA"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 8688..8705
/label="oTG775_pET_BbvCI_insert_seq"
/note="sequence: TTCCGCTTCCTCGCTCAC"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
rep_origin complement(8868..9455)
/label="ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 9509..9532
/label="oTG1180_AmpR_seq"
/note="sequence: ACGTTAAGGGATTTTGGTCATGAG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(9595..9625)
/label="oTG1177_FRT_AmpR_3’flanking"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer complement(9595..9625)
/label="oTG1554_pBS_diffusion_substrate_3kb"
/note="sequence: ctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 9596..9625
/label="oTG1182_E75_AmpR_F"
/note="sequence: gactaactgccactcgcagcgcgcagatcgAAGTATATATGAGTAAACTTGGTCTGACAG"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer 9625..9649
/label="oTG1549_pBS_849_adapter"
/note="sequence: GCTGTGTCTTATTACACACAGTCGttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
CDS complement(join(9626..9768,1..649))
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 9626..9649
/label="oTG1176_FRT_AmpR_3’"
/note="sequence: CTATTCTCTAGAAAGTATAGGAACTTCttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(9672..9694)
/label="oTG1550_pBS_diffusion_substrate_100bp"
/note="sequence: caggcaactatggatgaacgaaa"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC
61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG
121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA
181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA
241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA
301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA
361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA
421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG
481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG
541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG
601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG
661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC
721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT
901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA
961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA
1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA
1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG
1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA
1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG
1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG
1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC
1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG
1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG
1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG
1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT
1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA
1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT
1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT
1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT
1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA
1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC
2041 TGGGGGGATA CGGGGAAAAG GCGAACGCGT gggtagggga ggcgcttttc ccaaggcagt
2101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
2161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
2221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
2281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
2341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
2401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
2461 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
2521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
2581 caAGCTTCAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC
2641 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG
2701 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG
2761 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA
2821 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG
2881 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC
2941 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA
3001 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC
3061 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA
3121 CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC
3181 CCGGTGCCTG AGCGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AACGCGATCG CTCCGGTGCC
3481 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC
3541 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC
3601 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG
3661 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC
3721 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC
3781 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA
3841 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC
3901 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT
3961 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCCAC Catggacaaa
4021 gactgcgaaa tgaagcgcac caccctggat agccctctgg gcaagctgga actgtctggg
4081 tgcgaacagg gcctgcaccg tatcatcttc ctgggcaaag gaacatctgc cgccgacgcc
4141 gtggaagtgc ctgccccagc cgccgtgctg ggcggaccag agccactgat gcaggccacc
4201 gcctggctca acgcctactt tcaccagcct gaggccatcg aggagttccc tgtgccagcc
4261 ctgcaccacc cagtgttcca gcaggagagc tttacccgcc aggtgctgtg gaaactgctg
4321 aaagtggtga agttcggaga ggtcatcagc tacagccacc tggccgccct ggccggcaat
4381 cccgccgcca ccgccgccgt gaaaaccgcc ctgagcggaa atcccgtgcc cattctgatc
4441 ccctgccacc gggtggtgca gggcgacctg gacgtggggg gctacgaggg cgggctcgcc
4501 gtgaaagagt ggctgctggc ccacgagggc cacagactgg gcaagcctgg gctgggtGAG
4561 ATTTCCGGCa gcggagatgg cgctggactg atcaacggcC GAGCTGATCC AAAAAAGAAG
4621 AGAAAGGTAG ATCCAAAAAA GAAGAGAAAG GTAGATCCAA AAAAGAAGAG AAAGGTAGGA
4681 TCCACCGGAc atatgggcag cggcgccaca aacttctctc tgctaaagca agcaggtgat
4741 gttgaagaaa accccgggcc tACTAGTGCT GGAAAGCTTg gctccggcga gggcagggga
4801 agtcttctaa catgcgggga cgtggaggaa aatcccggcc caGgtaccAT GCCTGAGCCT
4861 GCGAAGTCCG CTCCCGCCCC GAAGAAGGGC TCCAAGAAGG CCGTCACCAA GGCCCAGAAG
4921 AAGGACGGCA AGAAGCGCAA GCGCAGCCGC AAGGAGAGCT ACTCGGTGTA CGTGTACAAG
4981 GTGCTGAAAC AAGTGCACCC CGACACGGGC ATCTCCTCCA AGGCCATGGG CATCATGAAC
5041 TCGTTCGTGA ACGACATCTT CGAGCGCATC GCGGGCGAGG CGTCCCGCCT GGCGCATTAC
5101 AACAAGCGCT CGACCATCAC GTCCCGGGAG ATCCAGACGG CCGTGCGCCT GCTGCTGCCC
5161 GGGGAGCTGG CCAAGCACGC GGTGTCGGAG GGCACCAAGG CCGTCACCAA GTACACCAGC
5221 TCCAAGTCCG GAGGATCCTC CATGGCAGAA ATCGGTACTG GCTTTCCATT CGACCCCCAT
5281 TATGTGGAAG TCCTGGGCGA GCGCATGCAC TACGTCGATG TTGGTCCGCG CGATGGCACC
5341 CCTGTGCTGT TCCTGCACGG TAACCCGACC TCCTCCTACG TGTGGCGCAA CATCATCCCG
5401 CATGTTGCAC CGACCCATCG CTGCATTGCT CCAGACCTGA TCGGTATGGG CAAATCCGAC
5461 AAACCAGACC TGGGTTATTT CTTCGACGAC CACGTCCGCT TCATGGATGC CTTCATCGAA
5521 GCCCTGGGTC TGGAAGAGGT CGTCCTGGTC ATTCACGACT GGGGCTCCGC TCTGGGTTTC
5581 CACTGGGCCA AGCGCAATCC AGAGCGCGTC AAAGGTATTG CATTTATGGA GTTCATCCGC
5641 CCTATCCCGA CCTGGGACGA ATGGCCAGAA TTTGCCCGCG AGACCTTCCA GGCCTTCCGC
5701 ACCACCGACG TCGGCCGCAA GCTGATCATC GATCAGAACG TTTTTATCGA GGGTACGCTG
5761 CCGATGGGTG TCGTCCGCCC GCTGACTGAA GTCGAGATGG ACCATTACCG CGAGCCGTTC
5821 CTGAATCCTG TTGACCGCGA GCCACTGTGG CGCTTCCCAA ACGAGCTGCC AATCGCCGGT
5881 GAGCCAGCGA ACATCGTCGC GCTGGTCGAA GAATACATGG ACTGGCTGCA CCAGTCCCCT
5941 GTCCCGAAGC TGCTGTTCTG GGGCACCCCA GGCGTTCTGA TCCCACCGGC CGAAGCCGCT
6001 CGCCTGGCCA AAAGCCTGCC TAACTGCAAG GCTGTGGACA TCGGCCCGGG TCTGAATCTG
6061 CTGCAAGAAG ACAACCCGGA CCTGATCGGC AGCGAGATCG CGCGCTGGCT GTCGACGCTC
6121 GAGATTTCCG GCggtagtgg tacaggttct ATGGATAAAG ACTGTGAGAT GAAGCGGACA
6181 ACCCTTGATT CCCCTCTGGG GAAACTGGAG CTCAGTGGGT GCGAACAGGG GCTGCACAGA
6241 ATTATATTTC TTGGGAAGGG CACGTCTGCT GCAGATGCTG TCGAGGTCCC AGCCCCGGCT
6301 GCCGTGCTTG GTGGACCAGA GCCTCTCATG CAAGCTACTG CTTGGCTGAA CGCGTATTTC
6361 CACCAACCTG AAGCGATAGA AGAGTTCCCT GTTCCTGCTC TGCACCACCC GGTTTTCCAA
6421 CAGGAATCTT TCACGAGACA AGTATTGTGG AAACTTCTCA AAGTGGTCAA ATTCGGTGAA
6481 GTAATATCCT ATTCACACCT GGCAGCTCTC GCCGGGAACC CGGCGGCAAC CGCTGCGGTT
6541 AAAACTGCAC TCTCTGGTAA TCCTGTACCA ATACTTATAC CGTGCCACAG GGTTGTCCAA
6601 GGCGACTTGG ACGTTGGAGG GTATGAAGGG GGCCTCGCTG TGAAAGAGTG GCTTCTCGCG
6661 CACGAAGGTC ATCGGCTCGG TAAGCCAGGG CTCGGGGAAA TCTCAGGAAG TGGAGATGGC
6721 GCCGGATTGA TCAATGGACG GGCAGATgct agcCCGAAGA AAAAGCGGAA GGTCGATCCC
6781 AAAAAGAAGA GGAAAGTGGA CCCAAAGAAG AAACGGAAGG TAtaaGGATC CACCGGATCT
6841 AGATAACTGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC
6901 CTCCCACACC TCCCCCTGAA CCTGAACGCG GCCGCtggGT CGACAATCAA CCTCTGGATT
6961 ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG
7021 GATACGCTGC TTTAATGCCT TTGTATCATG CGTTAACTAA ACTTGTTTAT TGCAGCTTAT
7081 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG
7141 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GAATTGACTC
7201 AAATGATGTC AATTAGTCTA TCAGAAGCTC ATCTGGTCTC CCTTCCGGGG GACAAGACAT
7261 CCCTGTTTAA TATTTAAACA GCAGTGTTCC CAAACTGGGT TCTTATATCC CTTGCTCTGG
7321 TCAACCAGGT TGCAGGGTTT CCTGTCCTCA CAGGAACGAA GTCCCTAAAG AAACAGTGGC
7381 AGCCAGGTTT AGCCCCGGAA TTGACTGGAT TCCTTTTTTA GGGCCCATTG GTATGGCTTT
7441 TTCCCCGTAT CCCCCCAGGT GTCTGCAGGC TCAAAGAGCA GCGAGAAGCG TTCAGAGGAA
7501 AGCGATCCCG TGCCACCTTC CCCGTGCCCG GGCTGTCCCC GCACGCTGCC GGCTCGGGGA
7561 TGCGGGGGGA GCGCCGGACC GGAGCGGAGC CCCGGGCGGC TCGCTGCTGC CCCCTAGCGG
7621 GGGAGGGACG TAATTACATC CCTGGGGGCT TTGGGGGGGG GCTGTCCCTG ATATCTATAA
7681 CAAGAAATAT ATATATAATA AGTTATCACG TAAGTAGAAC ATGAAATAAC AATATAATTA
7741 TCGTATGAGT TAATCTTAAA GTCACGTAAA AGATAATCAT GCGTCATTTT GACTCACGCG
7801 GTCGTTATAG TTCAAAATCA GTGACACTTA CCGCATTGAC AAGCACGCCT CACGGGAGCT
7861 CCAAGCGGCG ACTGAGATGT CCTAAATGCA CAGCGACGGA TTCGCGCTAT TTAGAAAGAG
7921 AGAGCAATAT TTCAAGAATG CATGCGTCAA TTTTACGCAG ACTATCTTTC TAGGGTTAAT
7981 CTAGCTGCAT CAGGATCATA TCGTCGGGTC TTTTTTCCGG CTCAGTCATC GCCCAAGCTG
8041 GCGCTATCTG GGCATCGGGG AGGAAGAAGC CCGTGCCTTT TCCCGCGAGG TTGAAGCGGC
8101 ATGGAAAGAG TTTGCCGAGG ATGACTGCTG CTGCATTGAC GTTGAGCGAA AACGCACGTT
8161 TACCATGATG ATTCGGGAAG GTGTGGCCAT GCACGCCTTT AACGGTGAAC TGTTCGTTCA
8221 GGCCACCTGG GATACCAGTT CGTCGCGGCT TTTCCGGACA CAGTTCCGGA TGGTCAGCCC
8281 GAAGCGCATC AGCAACCCGA ACAATACCGG CGACAGCCGG AACTGCCGTG CCGGTGTGCA
8341 GATTAATGAC AGCGGTGCGG CGCTGGGATA TTACGTCAGC GAGGACGGGT ATCCTGGCTG
8401 GATGCCGCAG AAATGGACAT GGATACCCCG TGAGTTACCC GGCGGGCGCG CTTGGCGTAA
8461 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA
8521 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
8581 ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA
8641 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG
8701 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG
8761 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA
8821 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC
8881 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
8941 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG
9001 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
9061 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
9121 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
9181 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
9241 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC
9301 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAGAG
9361 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA
9421 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG
9481 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA
9541 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA
9601 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG
9661 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA
9721 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAG
//