https://github.com/arjunrajlaboratory/RajLabSeqTools
Tip revision: c8b8c79b2ec9c1bd9eb7ced427bb2aec25f19506 authored by Benjamin Emert on 26 March 2020, 17:37:11 UTC
Updated reorganizeBasespaceFiles.py to better parse samples with same first index (e.g. sample 1 and sample10)
Updated reorganizeBasespaceFiles.py to better parse samples with same first index (e.g. sample 1 and sample10)
Tip revision: c8b8c79
prepareSamForHTSeq.sh
#!
# run from within "repo" directory
EXPERIMENT=$1
SAMPLEID=$2
ALIGNMENT_TOOL_NAME=rum
if [[ ! -z "$3" ]]; then
ALIGNMENT_TOOL_NAME="$3"
fi
commandName=prepareSamForHTSeq
if [ ! -d $EXPERIMENT/analyzed/$SAMPLEID/log ]; then
mkdir $EXPERIMENT/analyzed/$SAMPLEID/log
fi
JOURNAL=$EXPERIMENT/analyzed/$SAMPLEID/log/$(date +%Y-%m-%d_%H-%M).$commandName.log
if [ ! -d $EXPERIMENT/analyzed/$SAMPLEID/htseq ]; then
mkdir $EXPERIMENT/analyzed/$SAMPLEID/htseq
fi
readNameSortCmd="samtools sort -n \
$EXPERIMENT/analyzed/$SAMPLEID/$ALIGNMENT_TOOL_NAME/$SAMPLEID.sorted.mapped.unique.bam \
$EXPERIMENT/analyzed/$SAMPLEID/htseq/$SAMPLEID.nameSorted"
fixMateCmd="samtools fixmate \
$EXPERIMENT/analyzed/$SAMPLEID/htseq/$SAMPLEID.nameSorted.bam \
$EXPERIMENT/analyzed/$SAMPLEID/htseq/$SAMPLEID.nameSorted.mateFixed.bam"
toSamCmd="samtools view -h \
$EXPERIMENT/analyzed/$SAMPLEID/htseq/$SAMPLEID.nameSorted.mateFixed.bam \
> $EXPERIMENT/analyzed/$SAMPLEID/htseq/$SAMPLEID.nameSorted.mateFixed.sam"
echo "Starting..." >> $JOURNAL
date >> $JOURNAL
echo "$readNameSortCmd" >> $JOURNAL
eval "$readNameSortCmd"
echo "$fixMateCmd" >> $JOURNAL
eval "$fixMateCmd"
echo "$toSamCmd" >> $JOURNAL
eval "$toSamCmd"
date >> $JOURNAL
echo "Done" >> $JOURNAL