https://gitlab.com/tgwgraham/papa_paper_plasmids
Tip revision: 984803582c0023a9fe975ec6d41be3d723c2737b authored by Thomas Graham on 08 July 2022, 01:06:31 UTC
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Tip revision: 9848035
ptg838-ef1alpha-h2b-halo-snapf-3xnls.gb
LOCUS pTG838_EF1alpha-H2B-Hal 8933 bp ds-DNA circular 03-NOV-2021
DEFINITION .
KEYWORDS "creator:Gina Dailey - Darzacq/Tjian Lab" "marker:AmpR, PuroR"
FEATURES Location/Qualifiers
misc_feature 7..22
/label="IR (inverted repeat) truncated?"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
promoter 441..940
/label="PGK promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS 962..1561
/label="PuroR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
polyA_signal 1594..1818
/label="bGH poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
promoter 1858..2069
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter 1858..2069
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
LTR 2082..2350
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
LTR 2082..2350
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 2361..2380
/label="oTG1576_3xFlag-Halo_upstream_F"
/note="sequence: CCGGCGCCTACTCTAGCCAC"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 2362..2399
/label="oTG1626_H2B-Halo-SNAP-TP2A-SNAP_F"
/note="sequence: cggcgcctactctagccaccATGCCTGAGCCTGCGAAG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 2379..2399
/label="oTG1628_3xF-HaloSNAP-3xN-TP2A-H2B-SNAP_F"
/note="sequence: gaaaatcccggcccaGgtaccATGCCTGAGCCTGCGAAG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
CDS 2382..2759
/label="H2be"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 2382..4370
/label="Translation 2382-4370"
primer complement(2753..2771)
/label="oTG1629_3xF-HaloSNAP-3xN-TP2A-H2B-SNAP_R"
/note="sequence: tccacttccgccaccggtaccGGATCCTCCGGACTTGGAG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(2753..2797)
/label="oTG1627_H2B-Halo-SNAP-TP2A-SNAP_R"
/note="sequence: aagccagtaccgatttctgccatggaGGATCCTCCGGACTTGGAG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 2774..2799
/label="oTG945_60xHalo_F"
/note="sequence: CCGGCTCCGGCAGCGGTAGCATGGCAGAAATCGGTACTGGCTTTC"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 2775..2795
/label="oTG1573_GSGSKCK_Halo_F"
/note="sequence: GGTAGTGGTAGTAAGTGTAAAATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer 2775..2795
/label="oTG1582_SNAPf_linker_Halo_F"
/note="sequence: TGGGCAAGCCTGGGCTGGGTggtagtggtaccggttctATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 2775..2795
/label="oTG778_5'Halo"
/note="sequence: ATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 2775..2795
/label="oTG898_H10_Halo_F"
/note="sequence: TTCTCACCATCACCATCACCACCACCACCACCATATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
CDS 2775..3665
/label="HaloTag®"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 2778..2798
/label="oTG1144_inVitro_60xHalo_F"
/note="sequence: GGCTCCGGCAGCGGTAGCGCAGAAATCGGTACTGGCTTT"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer 2781..2803
/label="oTG995_Halo_linker_F"
/note="sequence: GATCTGGAGGTTCAGGAGGCAGCGGTGGAAGTGAAATCGGTACTGGCTTTCCATT"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer 2781..2803
/label="oTG1280_60mer_linker_Halo_F"
/note="sequence: GGAGTGGTAGCGGCAGTATTAAGgaaatcggtactggctttccatt"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(2786..2805)
/label="oTG1172_Halo_int_R2"
/note="sequence: CGAATGGAAAGCCAGTACCG"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(2791..2810)
/label="oTG1214_Halo_int_R_75"
/note="sequence: GGGGTCGAATGGAAAGCCAG"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(2814..2833)
/label="oTG1096_Halo_int_R"
/note="sequence: TCGCCCAGGACTTCCACATA"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(2835..2854)
/label="oTG1173_Halo_int_R3"
/note="sequence: ACATCGACGTAGTGCATGCG"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 2996..3017
/label="oTG1513_Halo_qPCR_F1"
/note="sequence: ACCAGACCTGGGTTATTTCTTC"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer 3136..3155
/label="oTG1208_Halo_int_F"
/note="sequence: AGCGCGTCAAAGGTATTGCA"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(3156..3175)
/label="oTG1514_Halo_qPCR_R1"
/note="sequence: GGGCGGATGAACTCCATAAA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(3250..3272)
/label="oTG1691_Halo_V167C_R"
/note="sequence: GTTCTGATCGATGATCAGCTTGC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(3250..3272)
/label="oTG1674_Halo_V167C_R"
/note="sequence: GTTCTGATCGATGATCAGCTTGC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(3259..3284)
/label="oTG1693_Halo_G171C_R"
/note="sequence: CTCGATAAAAACGTTCTGATCGATGA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(3259..3284)
/label="oTG1676_Halo_G171C_R"
/note="sequence: CTCGATAAAAACGTTCTGATCGATGA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 3275..3295
/label="oTG1690_Halo_V167C_F"
/note="sequence: tgtTTTATCGAGGGTACGCTGCC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 3275..3295
/label="oTG1673_Halo_V167C_F"
/note="sequence: tgtTTTATCGAGGGTACGCTGCC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(3277..3296)
/label="oTG1695_Halo_M175C_R"
/note="sequence: CGGCAGCGTACCCTCGATAA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(3277..3296)
/label="oTG1678_Halo_M175C_R"
/note="sequence: CGGCAGCGTACCCTCGATAA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 3286..3305
/label="oTG1692_Halo_G171C_F"
/note="sequence: tgtACGCTGCCGATGGGTGTC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 3286..3305
/label="oTG1675_Halo_G171C_F"
/note="sequence: tgtACGCTGCCGATGGGTGTC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 3300..3317
/label="oTG1677_Halo_M175C_F"
/note="sequence: tgtGGTGTCGTCCGCCCGCTG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 3300..3317
/label="oTG1694_Halo_M175C_F"
/note="sequence: tgtGGTGTCGTCCGCCCGCTG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(3314..3335)
/label="oTG1207_Halo_int_R"
/note="sequence: GTCCATCTCGACTTCAGTCAGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 3321..3342
/label="oTG1515_Halo_qPCR_F2"
/note="sequence: GAAGTCGAGATGGACCATTACC"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer complement(3443..3462)
/label="oTG1516_Halo_qPCR_R2"
/note="sequence: GCAGCCAGTCCATGTATTCT"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 3556..3575
/label="oTG1095_Halo_int_F"
/note="sequence: ACTGCAAGGCTGTGGACATC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 3583..3605
/label="oTG979_3'_Halo_seq"
/note="sequence: GTCTGAATCTGCTGCAAGAAGAC"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer complement(3633..3650)
/label="oTG1312_Halo_tubUTR_R"
/note="sequence: cgcgacgcttagtgtgccgcTTACGTCGACAGCCAGCGCGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(3645..3664)
/label="oTG1261_ParB2_Halo_stop_R"
/note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(3645..3664)
/label="oTG1261_ParB2_Halo_stop_R"
/note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(3646..3665)
/label="oTG793_3'Halo_R"
/note="sequence: GCCGGAAATCTCGAGCGTCG"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer complement(3646..3665)
/label="oTG779_Halo-stop_pETSUMO"
/note="sequence: GCTCCAATTGGGTACCCATGGAGCCGGAAATCTCGAGCGTCG"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer complement(3646..3667)
/label="oTG1583_Halo_noNLS_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCgccggaaatctcgagcgtcg"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 3657..3676
/label="Gibson overhang"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 3657..3676
/label="oTG1618_SNAP-3xNLS_gBlock_F"
/note="sequence: ATTTCCGGCggtagtggtac"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 3684..4229
/label="SNAPf"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 4235..4254
/label="oTG1683_3xNLS-P2A-T2A_KpnI_F"
/note="sequence: atttccggcggtagtggtaccTCAGGAAGTGGAGATGGCG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 4287..4355
/label="3xNLS"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 4359..4383
/label="oTG967_pHalo-Stop_F"
/note="sequence: TAGGATCCACCGGATCTAGATAACT"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
misc_feature 4375..4385
/label="stop codons"
/ApEinfo_revcolor=#ff0000
/ApEinfo_fwdcolor=#ff0000
misc_feature 4462..4462
/label="add_before"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 4462..4479
/label="MCS"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
polyA_signal 4595..4716
/label="SV40 poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(4688..4716)
/label="oTG885_SV40_pA_F"
/note="sequence: TAAGATACATTGATGAGTTTGGACAAACC"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature complement(5476..5494)
/label="IR (inverted repeat)"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
misc_feature complement(5498..5510)
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind complement(6001..6017)
/label="M13 rev"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
protein_bind 6025..6041
/label="lac operator"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
promoter complement(6049..6055)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(6056..6073)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(6067..6088)
/label="oTG980_Vector-Fwd-GA"
/note="sequence: ccggatgacagtgacgaggagaactgaACCCCAGGCTTTacacTTTATG"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
promoter complement(6074..6079)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
protein_bind 6094..6115
/label="CAP binding site"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
primer 6146..6166
/label="oTG1156_mCherry-LacI-Rev"
/note="sequence: AAGCTTCCATTCGGGAAACCTGTCGtgCCAG"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 6166..6186
/label="oTG1089_Rev-LacI-NLS-NOSTOP-GA"
/note="sequence: AACCTTCCTCTTCTTCTTAGGAGGCTGCATTAATGAATCGGCCA"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 6166..6187
/label="oTG1084_Rev-LacI-GA"
/note="sequence: TCAAACCTTCCTCTtcTTcTTaGgagGCTGCATTAATGAATCGGCCAA"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 6223..6240
/label="oTG775_pET_BbvCI_insert_seq"
/note="sequence: TTCCGCTTCCTCGCTCAC"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
rep_origin complement(6403..6990)
/label="ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 7044..7067
/label="oTG1180_AmpR_seq"
/note="sequence: ACGTTAAGGGATTTTGGTCATGAG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(7130..7160)
/label="oTG1177_FRT_AmpR_3’flanking"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer complement(7130..7160)
/label="oTG1554_pBS_diffusion_substrate_3kb"
/note="sequence: ctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 7131..7160
/label="oTG1182_E75_AmpR_F"
/note="sequence: gactaactgccactcgcagcgcgcagatcgAAGTATATATGAGTAAACTTGGTCTGACAG"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer 7160..7184
/label="oTG1549_pBS_849_adapter"
/note="sequence: GCTGTGTCTTATTACACACAGTCGttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer 7161..7184
/label="oTG1176_FRT_AmpR_3’"
/note="sequence: CTATTCTCTAGAAAGTATAGGAACTTCttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
CDS complement(7161..7952)
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(7207..7229)
/label="oTG1550_pBS_diffusion_substrate_100bp"
/note="sequence: caggcaactatggatgaacgaaa"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(7411..7429)
/label="oTG1552_pBS_diffusion_substrate_300bp"
/note="sequence: ctctagcttcccggcaaca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS complement(7953..8021)
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter complement(8022..8126)
/label="AmpR promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(8105..8126)
/label="oTG1179_FRT_AmpR_5’"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCcgcggaacccctatttgtttat"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(8124..8142)
/label="oTG1553_pBS_diffusion_substrate_1kb"
/note="sequence: ttttcggggaaatgtgcgc"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
rep_origin complement(8152..8606)
/label="f1 ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 8744..8763
/label="oTG1336_M13F_extended"
/note="sequence: GTTGTAAAACGACGGCCAGT"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind 8746..8763
/label="M13-fwd"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 8747..8763
/label="M13 fwd"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
misc_feature 8893..8905
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
ORIGIN
1 TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT ATTTATTAAT
61 TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA ATTCAACAAA
121 CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT TCTTCTATAA
181 AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT GTAATTACGT
241 CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT CCGGCGCTCC
301 CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA GGTGGCACGG
361 GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC TGGGGGGATA
421 CGGGGAAAAG GCGAACGCGT gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
481 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
541 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
601 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
661 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
721 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
781 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
841 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
901 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caAGCTTCAC
961 CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT
1021 ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA
1081 CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA
1141 CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA
1201 GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG
1261 TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA
1321 GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG
1381 CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT
1441 GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC
1501 CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG
1561 AGCGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG
1621 TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT
1681 CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG
1741 GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG
1801 ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AACGCGATCG CTCCGGTGCC CGTCAGTGGG
1861 CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACGG
1921 GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC
1981 TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT
2041 TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC ATCTCTCCTT
2101 CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC GTTCTGCCGC
2161 CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA AAGCTCAGGT
2221 CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC CGGCTCTCCA
2281 CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT GTTCTGCGCC
2341 GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCCAC CATGCCTGAG CCTGCGAAGT
2401 CCGCTCCCGC CCCGAAGAAG GGCTCCAAGA AGGCCGTCAC CAAGGCCCAG AAGAAGGACG
2461 GCAAGAAGCG CAAGCGCAGC CGCAAGGAGA GCTACTCGGT GTACGTGTAC AAGGTGCTGA
2521 AACAAGTGCA CCCCGACACG GGCATCTCCT CCAAGGCCAT GGGCATCATG AACTCGTTCG
2581 TGAACGACAT CTTCGAGCGC ATCGCGGGCG AGGCGTCCCG CCTGGCGCAT TACAACAAGC
2641 GCTCGACCAT CACGTCCCGG GAGATCCAGA CGGCCGTGCG CCTGCTGCTG CCCGGGGAGC
2701 TGGCCAAGCA CGCGGTGTCG GAGGGCACCA AGGCCGTCAC CAAGTACACC AGCTCCAAGT
2761 CCGGAGGATC CTCCATGGCA GAAATCGGTA CTGGCTTTCC ATTCGACCCC CATTATGTGG
2821 AAGTCCTGGG CGAGCGCATG CACTACGTCG ATGTTGGTCC GCGCGATGGC ACCCCTGTGC
2881 TGTTCCTGCA CGGTAACCCG ACCTCCTCCT ACGTGTGGCG CAACATCATC CCGCATGTTG
2941 CACCGACCCA TCGCTGCATT GCTCCAGACC TGATCGGTAT GGGCAAATCC GACAAACCAG
3001 ACCTGGGTTA TTTCTTCGAC GACCACGTCC GCTTCATGGA TGCCTTCATC GAAGCCCTGG
3061 GTCTGGAAGA GGTCGTCCTG GTCATTCACG ACTGGGGCTC CGCTCTGGGT TTCCACTGGG
3121 CCAAGCGCAA TCCAGAGCGC GTCAAAGGTA TTGCATTTAT GGAGTTCATC CGCCCTATCC
3181 CGACCTGGGA CGAATGGCCA GAATTTGCCC GCGAGACCTT CCAGGCCTTC CGCACCACCG
3241 ACGTCGGCCG CAAGCTGATC ATCGATCAGA ACGTTTTTAT CGAGGGTACG CTGCCGATGG
3301 GTGTCGTCCG CCCGCTGACT GAAGTCGAGA TGGACCATTA CCGCGAGCCG TTCCTGAATC
3361 CTGTTGACCG CGAGCCACTG TGGCGCTTCC CAAACGAGCT GCCAATCGCC GGTGAGCCAG
3421 CGAACATCGT CGCGCTGGTC GAAGAATACA TGGACTGGCT GCACCAGTCC CCTGTCCCGA
3481 AGCTGCTGTT CTGGGGCACC CCAGGCGTTC TGATCCCACC GGCCGAAGCC GCTCGCCTGG
3541 CCAAAAGCCT GCCTAACTGC AAGGCTGTGG ACATCGGCCC GGGTCTGAAT CTGCTGCAAG
3601 AAGACAACCC GGACCTGATC GGCAGCGAGA TCGCGCGCTG GCTGTCGACG CTCGAGATTT
3661 CCGGCggtag tggtacaggt tctATGGATA AAGACTGTGA GATGAAGCGG ACAACCCTTG
3721 ATTCCCCTCT GGGGAAACTG GAGCTCAGTG GGTGCGAACA GGGGCTGCAC AGAATTATAT
3781 TTCTTGGGAA GGGCACGTCT GCTGCAGATG CTGTCGAGGT CCCAGCCCCG GCTGCCGTGC
3841 TTGGTGGACC AGAGCCTCTC ATGCAAGCTA CTGCTTGGCT GAACGCGTAT TTCCACCAAC
3901 CTGAAGCGAT AGAAGAGTTC CCTGTTCCTG CTCTGCACCA CCCGGTTTTC CAACAGGAAT
3961 CTTTCACGAG ACAAGTATTG TGGAAACTTC TCAAAGTGGT CAAATTCGGT GAAGTAATAT
4021 CCTATTCACA CCTGGCAGCT CTCGCCGGGA ACCCGGCGGC AACCGCTGCG GTTAAAACTG
4081 CACTCTCTGG TAATCCTGTA CCAATACTTA TACCGTGCCA CAGGGTTGTC CAAGGCGACT
4141 TGGACGTTGG AGGGTATGAA GGGGGCCTCG CTGTGAAAGA GTGGCTTCTC GCGCACGAAG
4201 GTCATCGGCT CGGTAAGCCA GGGCTCGGGG AAATCTCAGG AAGTGGAGAT GGCGCCGGAT
4261 TGATCAATGG ACGGGCAGAT gctagcCCGA AGAAAAAGCG GAAGGTCGAT CCCAAAAAGA
4321 AGAGGAAAGT GGACCCAAAG AAGAAACGGA AGGTAcataG CGGTACCTAG TGACTAGATA
4381 ACTGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC
4441 ACACCTCCCC CTGAACCTGA ACGCGGCCGC tggGTCGACA ATCAACCTCT GGATTACAAA
4501 ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC
4561 GCTGCTTTAA TGCCTTTGTA TCATGCGTTA ACTAAACTTG TTTATTGCAG CTTATAATGG
4621 TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC
4681 TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGAATT GACTCAAATG
4741 ATGTCAATTA GTCTATCAGA AGCTCATCTG GTCTCCCTTC CGGGGGACAA GACATCCCTG
4801 TTTAATATTT AAACAGCAGT GTTCCCAAAC TGGGTTCTTA TATCCCTTGC TCTGGTCAAC
4861 CAGGTTGCAG GGTTTCCTGT CCTCACAGGA ACGAAGTCCC TAAAGAAACA GTGGCAGCCA
4921 GGTTTAGCCC CGGAATTGAC TGGATTCCTT TTTTAGGGCC CATTGGTATG GCTTTTTCCC
4981 CGTATCCCCC CAGGTGTCTG CAGGCTCAAA GAGCAGCGAG AAGCGTTCAG AGGAAAGCGA
5041 TCCCGTGCCA CCTTCCCCGT GCCCGGGCTG TCCCCGCACG CTGCCGGCTC GGGGATGCGG
5101 GGGGAGCGCC GGACCGGAGC GGAGCCCCGG GCGGCTCGCT GCTGCCCCCT AGCGGGGGAG
5161 GGACGTAATT ACATCCCTGG GGGCTTTGGG GGGGGGCTGT CCCTGATATC TATAACAAGA
5221 AATATATATA TAATAAGTTA TCACGTAAGT AGAACATGAA ATAACAATAT AATTATCGTA
5281 TGAGTTAATC TTAAAGTCAC GTAAAAGATA ATCATGCGTC ATTTTGACTC ACGCGGTCGT
5341 TATAGTTCAA AATCAGTGAC ACTTACCGCA TTGACAAGCA CGCCTCACGG GAGCTCCAAG
5401 CGGCGACTGA GATGTCCTAA ATGCACAGCG ACGGATTCGC GCTATTTAGA AAGAGAGAGC
5461 AATATTTCAA GAATGCATGC GTCAATTTTA CGCAGACTAT CTTTCTAGGG TTAATCTAGC
5521 TGCATCAGGA TCATATCGTC GGGTCTTTTT TCCGGCTCAG TCATCGCCCA AGCTGGCGCT
5581 ATCTGGGCAT CGGGGAGGAA GAAGCCCGTG CCTTTTCCCG CGAGGTTGAA GCGGCATGGA
5641 AAGAGTTTGC CGAGGATGAC TGCTGCTGCA TTGACGTTGA GCGAAAACGC ACGTTTACCA
5701 TGATGATTCG GGAAGGTGTG GCCATGCACG CCTTTAACGG TGAACTGTTC GTTCAGGCCA
5761 CCTGGGATAC CAGTTCGTCG CGGCTTTTCC GGACACAGTT CCGGATGGTC AGCCCGAAGC
5821 GCATCAGCAA CCCGAACAAT ACCGGCGACA GCCGGAACTG CCGTGCCGGT GTGCAGATTA
5881 ATGACAGCGG TGCGGCGCTG GGATATTACG TCAGCGAGGA CGGGTATCCT GGCTGGATGC
5941 CGCAGAAATG GACATGGATA CCCCGTGAGT TACCCGGCGG GCGCGCTTGG CGTAATCATG
6001 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC
6061 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC
6121 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT
6181 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC
6241 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT
6301 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA
6361 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC
6421 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
6481 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
6541 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
6601 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
6661 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
6721 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
6781 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
6841 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAGAGTTGGT
6901 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
6961 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
7021 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
7081 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
7141 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
7201 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
7261 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGTT
7321 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
7381 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
7441 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
7501 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
7561 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
7621 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
7681 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
7741 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
7801 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
7861 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
7921 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
7981 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
8041 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
8101 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TAAATTGTAA
8161 GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC
8221 AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA
8281 GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG
8341 GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT
8401 TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG
8461 AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC
8521 GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC
8581 GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA
8641 AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC
8701 AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TCCCAGTCAC GACGTTGTAA AACGACGGCC
8761 AGTGAGCGCG CCTCGTTCAT TCACGTTTTT GAACCCGTGG AGGACGGGCA GACTCGCGGT
8821 GCAAATGTGT TTTACAGCGT GATGGAGCAG ATGAAGATGC TCGACACGCT GCAGAACACG
8881 CAGCTAGATT AACCCTAGAA AGATAATCAT ATTGTGACGT ACGTTAAAGA TAA
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