LOCUS pTG765_EF1alpha-FRB-Hal 8259 bp ds-DNA circular 27-JAN-2021 DEFINITION . KEYWORDS "creator:Gina Dailey - Darzacq/Tjian Lab" "marker:AmpR, PuroR" FEATURES Location/Qualifiers primer complement(108..126) /label="oTG1552_pBS_diffusion_substrate_300bp" /note="sequence: ctctagcttcccggcaaca" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 CDS complement(650..718) /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 promoter complement(719..823) /label="AmpR promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(802..823) /label="oTG1179_FRT_AmpR_5’" /note="sequence: CTATACTTTCTAGAGAATAGGAACTTCcgcggaacccctatttgtttat" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer complement(821..839) /label="oTG1553_pBS_diffusion_substrate_1kb" /note="sequence: ttttcggggaaatgtgcgc" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 rep_origin complement(849..1303) /label="f1 ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer 1441..1460 /label="oTG1336_M13F_extended" /note="sequence: GTTGTAAAACGACGGCCAGT" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer_bind 1443..1460 /label="M13-fwd" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind 1444..1460 /label="M13 fwd" /ApEinfo_revcolor=#a020f0 /ApEinfo_fwdcolor=#a020f0 misc_feature 1590..1602 /label="PB Terminal repeat" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1637..1652 /label="IR (inverted repeat) truncated?" /ApEinfo_revcolor=#339966 /ApEinfo_fwdcolor=#339966 promoter 2071..2570 /label="PGK promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS 2592..3191 /label="PuroR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 polyA_signal 3224..3448 /label="bGH poly(A) signal" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac promoter 3488..3699 /label="EF-1α core promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 3488..3699 /label="EF-1α core promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 LTR 3712..3980 /label="5' LTR (truncated)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 LTR 3712..3980 /label="5' LTR (truncated)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer 3991..4010 /label="oTG1576_3xFlag-Halo_upstream_F" /note="sequence: CCGGCGCCTACTCTAGCCAC" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer 3991..4035 /label="oTG1591_FRB_Halo_F" /note="sequence: CCGGCGCCTACTCTAGCCACCATGatcctctggcatgagatgtgg" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd CDS 4012..5341 /label="Translation 4012-5341" misc_feature 4015..4028 /label="F primer" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 CDS 4015..4293 /label="FRB*" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 misc_feature complement(4272..4293) /label="R primer" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(4292..4341) /label="oTG1592_FRB_Halo_R" /note="sequence: AAAGCCAGTACCGATTTCTGCCATGGAGCCtccgctgccggaaatctcct" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS 4309..4312 /label="GDGAGLIN linker" /ApEinfo_revcolor=#cd4489 /ApEinfo_fwdcolor=#cd4489 primer 4317..4342 /label="oTG945_60xHalo_F" /note="sequence: CCGGCTCCGGCAGCGGTAGCATGGCAGAAATCGGTACTGGCTTTC" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer 4318..4338 /label="oTG898_H10_Halo_F" /note="sequence: TTCTCACCATCACCATCACCACCACCACCACCATATGGCAGAAATCGGTACTGGC" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 4318..4338 /label="oTG1573_GSGSKCK_Halo_F" /note="sequence: GGTAGTGGTAGTAAGTGTAAAATGGCAGAAATCGGTACTGGC" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer 4318..4338 /label="oTG1582_SNAPf_linker_Halo_F" /note="sequence: TGGGCAAGCCTGGGCTGGGTggtagtggtaccggttctATGGCAGAAATCGGTACTGGC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 4318..4338 /label="oTG778_5'Halo" /note="sequence: ATGGCAGAAATCGGTACTGGC" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e CDS 4318..5208 /label="HaloTag®" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer 4321..4341 /label="oTG1144_inVitro_60xHalo_F" /note="sequence: GGCTCCGGCAGCGGTAGCGCAGAAATCGGTACTGGCTTT" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer 4324..4346 /label="oTG1280_60mer_linker_Halo_F" /note="sequence: GGAGTGGTAGCGGCAGTATTAAGgaaatcggtactggctttccatt" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer 4324..4346 /label="oTG995_Halo_linker_F" /note="sequence: GATCTGGAGGTTCAGGAGGCAGCGGTGGAAGTGAAATCGGTACTGGCTTTCCATT" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer complement(4329..4348) /label="oTG1172_Halo_int_R2" /note="sequence: CGAATGGAAAGCCAGTACCG" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer complement(4334..4353) /label="oTG1214_Halo_int_R_75" /note="sequence: GGGGTCGAATGGAAAGCCAG" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer complement(4357..4376) /label="oTG1096_Halo_int_R" /note="sequence: TCGCCCAGGACTTCCACATA" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer complement(4378..4397) /label="oTG1173_Halo_int_R3" /note="sequence: ACATCGACGTAGTGCATGCG" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer 4539..4560 /label="oTG1513_Halo_qPCR_F1" /note="sequence: ACCAGACCTGGGTTATTTCTTC" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer 4679..4698 /label="oTG1208_Halo_int_F" /note="sequence: AGCGCGTCAAAGGTATTGCA" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer complement(4699..4718) /label="oTG1514_Halo_qPCR_R1" /note="sequence: GGGCGGATGAACTCCATAAA" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer complement(4857..4878) /label="oTG1207_Halo_int_R" /note="sequence: GTCCATCTCGACTTCAGTCAGC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 4864..4885 /label="oTG1515_Halo_qPCR_F2" /note="sequence: GAAGTCGAGATGGACCATTACC" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer complement(4986..5005) /label="oTG1516_Halo_qPCR_R2" /note="sequence: GCAGCCAGTCCATGTATTCT" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer 5099..5118 /label="oTG1095_Halo_int_F" /note="sequence: ACTGCAAGGCTGTGGACATC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 5126..5148 /label="oTG979_3'_Halo_seq" /note="sequence: GTCTGAATCTGCTGCAAGAAGAC" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer complement(5176..5193) /label="oTG1312_Halo_tubUTR_R" /note="sequence: cgcgacgcttagtgtgccgcTTACGTCGACAGCCAGCGCGC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer complement(5188..5207) /label="oTG1261_ParB2_Halo_stop_R" /note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(5188..5207) /label="oTG1261_ParB2_Halo_stop_R" /note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer complement(5189..5208) /label="oTG793_3'Halo_R" /note="sequence: GCCGGAAATCTCGAGCGTCG" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer complement(5189..5208) /label="oTG779_Halo-stop_pETSUMO" /note="sequence: GCTCCAATTGGGTACCCATGGAGCCGGAAATCTCGAGCGTCG" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer complement(5189..5208) /label="oTG1583_Halo_noNLS_R" /note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCgccggaaatctcgagcgtcg" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 5212..5232 /label="oTG1415_7-SspB-p65-Fwd-OptoFly-GA" /note="sequence: ggagatggcgctggactgatc" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 CDS 5212..5235 /label="GDGAGLIN linker" /ApEinfo_revcolor=#cd4489 /ApEinfo_fwdcolor=#cd4489 misc_feature 5238..5238 /label="add_after" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS 5248..5268 /label="SV40 NLS" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 CDS 5272..5292 /label="SV40 NLS" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 CDS 5296..5316 /label="SV40 NLS" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 primer 5315..5339 /label="oTG967_pHalo-Stop_F" /note="sequence: TAGGATCCACCGGATCTAGATAACT" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer complement(5322..5350) /label="oTG1579_3xNLS_downstream_R" /note="sequence: TGATTATGATCAGTTATCTAGATCCGGTG" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 5331..5341 /label="stop codons" /ApEinfo_revcolor=#ff0000 /ApEinfo_fwdcolor=#ff0000 misc_feature 5418..5418 /label="add_before" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS 5418..5435 /label="MCS" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc polyA_signal 5551..5672 /label="SV40 poly(A) signal" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer complement(5644..5672) /label="oTG885_SV40_pA_F" /note="sequence: TAAGATACATTGATGAGTTTGGACAAACC" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature complement(6432..6450) /label="IR (inverted repeat)" /ApEinfo_revcolor=#339966 /ApEinfo_fwdcolor=#339966 misc_feature complement(6454..6466) /label="PB Terminal repeat" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind complement(6957..6973) /label="M13 rev" /ApEinfo_revcolor=#a020f0 /ApEinfo_fwdcolor=#a020f0 protein_bind 6981..6997 /label="lac operator" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b promoter complement(7005..7011) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter complement(7012..7029) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(7023..7044) /label="oTG980_Vector-Fwd-GA" /note="sequence: ccggatgacagtgacgaggagaactgaACCCCAGGCTTTacacTTTATG" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e promoter complement(7030..7035) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 protein_bind 7050..7071 /label="CAP binding site" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b primer 7102..7122 /label="oTG1156_mCherry-LacI-Rev" /note="sequence: AAGCTTCCATTCGGGAAACCTGTCGtgCCAG" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer 7122..7142 /label="oTG1089_Rev-LacI-NLS-NOSTOP-GA" /note="sequence: AACCTTCCTCTTCTTCTTAGGAGGCTGCATTAATGAATCGGCCA" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer 7122..7143 /label="oTG1084_Rev-LacI-GA" /note="sequence: TCAAACCTTCCTCTtcTTcTTaGgagGCTGCATTAATGAATCGGCCAA" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer 7179..7196 /label="oTG775_pET_BbvCI_insert_seq" /note="sequence: TTCCGCTTCCTCGCTCAC" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 rep_origin complement(7359..7946) /label="ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer 8000..8023 /label="oTG1180_AmpR_seq" /note="sequence: ACGTTAAGGGATTTTGGTCATGAG" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(8086..8116) /label="oTG1177_FRT_AmpR_3’flanking" /note="sequence: CTATACTTTCTAGAGAATAGGAACTTCctgtcagaccaagtttactcatatatacttt" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer complement(8086..8116) /label="oTG1554_pBS_diffusion_substrate_3kb" /note="sequence: ctgtcagaccaagtttactcatatatacttt" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer 8087..8116 /label="oTG1182_E75_AmpR_F" /note="sequence: gactaactgccactcgcagcgcgcagatcgAAGTATATATGAGTAAACTTGGTCTGACAG" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer 8116..8140 /label="oTG1549_pBS_849_adapter" /note="sequence: GCTGTGTCTTATTACACACAGTCGttaccaatgcttaatcagtgaggc" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 CDS complement(join(8117..8259,1..649)) /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 8117..8140 /label="oTG1176_FRT_AmpR_3’" /note="sequence: CTATTCTCTAGAAAGTATAGGAACTTCttaccaatgcttaatcagtgaggc" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer complement(8163..8185) /label="oTG1550_pBS_diffusion_substrate_100bp" /note="sequence: caggcaactatggatgaacgaaa" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 ORIGIN 1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT 901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA 961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT 1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA 1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA 1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG 1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA 1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG 1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG 1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC 1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG 1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG 1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG 1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT 1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA 1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT 1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT 1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT 1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA 1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC 2041 TGGGGGGATA CGGGGAAAAG GCGAACGCGT gggtagggga ggcgcttttc ccaaggcagt 2101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc 2161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct 2221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt 2281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca 2341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct 2401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc 2461 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa 2521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc 2581 caAGCTTCAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC 2641 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG 2701 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG 2761 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA 2821 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG 2881 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC 2941 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA 3001 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC 3061 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA 3121 CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC 3181 CCGGTGCCTG AGCGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC 3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC 3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT 3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG 3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AACGCGATCG CTCCGGTGCC 3481 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC 3541 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC 3601 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG 3661 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC 3721 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC 3781 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA 3841 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC 3901 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT 3961 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCCAC CATGatcctc 4021 tggcatgaga tgtggcatga aggcctggaa gaggcatctc gtttgtactt tggggaaagg 4081 aacgtgaaag gcatgtttga ggtgctggag cccttgcatg ctatgatgga acggggcccc 4141 cagactctga aggaaacatc ctttaatcag gcctatggtc gagatttaat ggaggcccaa 4201 gagtggtgca ggaagtacat gaaatcaggg aatgtcaagg acctcctcca agcctgggac 4261 ctctattatc atgtgttccg acgaatctca aaggagattt ccggcagcgg aGGCTCCATG 4321 GCAGAAATCG GTACTGGCTT TCCATTCGAC CCCCATTATG TGGAAGTCCT GGGCGAGCGC 4381 ATGCACTACG TCGATGTTGG TCCGCGCGAT GGCACCCCTG TGCTGTTCCT GCACGGTAAC 4441 CCGACCTCCT CCTACGTGTG GCGCAACATC ATCCCGCATG TTGCACCGAC CCATCGCTGC 4501 ATTGCTCCAG ACCTGATCGG TATGGGCAAA TCCGACAAAC CAGACCTGGG TTATTTCTTC 4561 GACGACCACG TCCGCTTCAT GGATGCCTTC ATCGAAGCCC TGGGTCTGGA AGAGGTCGTC 4621 CTGGTCATTC ACGACTGGGG CTCCGCTCTG GGTTTCCACT GGGCCAAGCG CAATCCAGAG 4681 CGCGTCAAAG GTATTGCATT TATGGAGTTC ATCCGCCCTA TCCCGACCTG GGACGAATGG 4741 CCAGAATTTG CCCGCGAGAC CTTCCAGGCC TTCCGCACCA CCGACGTCGG CCGCAAGCTG 4801 ATCATCGATC AGAACGTTTT TATCGAGGGT ACGCTGCCGA TGGGTGTCGT CCGCCCGCTG 4861 ACTGAAGTCG AGATGGACCA TTACCGCGAG CCGTTCCTGA ATCCTGTTGA CCGCGAGCCA 4921 CTGTGGCGCT TCCCAAACGA GCTGCCAATC GCCGGTGAGC CAGCGAACAT CGTCGCGCTG 4981 GTCGAAGAAT ACATGGACTG GCTGCACCAG TCCCCTGTCC CGAAGCTGCT GTTCTGGGGC 5041 ACCCCAGGCG TTCTGATCCC ACCGGCCGAA GCCGCTCGCC TGGCCAAAAG CCTGCCTAAC 5101 TGCAAGGCTG TGGACATCGG CCCGGGTCTG AATCTGCTGC AAGAAGACAA CCCGGACCTG 5161 ATCGGCAGCG AGATCGCGCG CTGGCTGTCG ACGCTCGAGA TTTCCGGCag cggagatggc 5221 gctggactga tcaacggcCG AGCTGATCCA AAAAAGAAGA GAAAGGTAGA TCCAAAAAAG 5281 AAGAGAAAGG TAGATCCAAA AAAGAAGAGA AAGGTAGGAT CCACCGGATC TAGATAACTG 5341 ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC 5401 CTCCCCCTGA ACCTGAACGC GGCCGCtggG TCGACAATCA ACCTCTGGAT TACAAAATTT 5461 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 5521 CTTTAATGCC TTTGTATCAT GCGTTAACTA AACTTGTTTA TTGCAGCTTA TAATGGTTAC 5581 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT 5641 TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGAATTGACT CAAATGATGT 5701 CAATTAGTCT ATCAGAAGCT CATCTGGTCT CCCTTCCGGG GGACAAGACA TCCCTGTTTA 5761 ATATTTAAAC AGCAGTGTTC CCAAACTGGG TTCTTATATC CCTTGCTCTG GTCAACCAGG 5821 TTGCAGGGTT TCCTGTCCTC ACAGGAACGA AGTCCCTAAA GAAACAGTGG CAGCCAGGTT 5881 TAGCCCCGGA ATTGACTGGA TTCCTTTTTT AGGGCCCATT GGTATGGCTT TTTCCCCGTA 5941 TCCCCCCAGG TGTCTGCAGG CTCAAAGAGC AGCGAGAAGC GTTCAGAGGA AAGCGATCCC 6001 GTGCCACCTT CCCCGTGCCC GGGCTGTCCC CGCACGCTGC CGGCTCGGGG ATGCGGGGGG 6061 AGCGCCGGAC CGGAGCGGAG CCCCGGGCGG CTCGCTGCTG CCCCCTAGCG GGGGAGGGAC 6121 GTAATTACAT CCCTGGGGGC TTTGGGGGGG GGCTGTCCCT GATATCTATA ACAAGAAATA 6181 TATATATAAT AAGTTATCAC GTAAGTAGAA CATGAAATAA CAATATAATT ATCGTATGAG 6241 TTAATCTTAA AGTCACGTAA AAGATAATCA TGCGTCATTT TGACTCACGC GGTCGTTATA 6301 GTTCAAAATC AGTGACACTT ACCGCATTGA CAAGCACGCC TCACGGGAGC TCCAAGCGGC 6361 GACTGAGATG TCCTAAATGC ACAGCGACGG ATTCGCGCTA TTTAGAAAGA GAGAGCAATA 6421 TTTCAAGAAT GCATGCGTCA ATTTTACGCA GACTATCTTT CTAGGGTTAA TCTAGCTGCA 6481 TCAGGATCAT ATCGTCGGGT CTTTTTTCCG GCTCAGTCAT CGCCCAAGCT GGCGCTATCT 6541 GGGCATCGGG GAGGAAGAAG CCCGTGCCTT TTCCCGCGAG GTTGAAGCGG CATGGAAAGA 6601 GTTTGCCGAG GATGACTGCT GCTGCATTGA CGTTGAGCGA AAACGCACGT TTACCATGAT 6661 GATTCGGGAA GGTGTGGCCA TGCACGCCTT TAACGGTGAA CTGTTCGTTC AGGCCACCTG 6721 GGATACCAGT TCGTCGCGGC TTTTCCGGAC ACAGTTCCGG ATGGTCAGCC CGAAGCGCAT 6781 CAGCAACCCG AACAATACCG GCGACAGCCG GAACTGCCGT GCCGGTGTGC AGATTAATGA 6841 CAGCGGTGCG GCGCTGGGAT ATTACGTCAG CGAGGACGGG TATCCTGGCT GGATGCCGCA 6901 GAAATGGACA TGGATACCCC GTGAGTTACC CGGCGGGCGC GCTTGGCGTA ATCATGGTCA 6961 TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA 7021 AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG 7081 CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC 7141 CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC 7201 TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA 7261 CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA 7321 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT 7381 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA 7441 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG 7501 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA 7561 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 7621 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG 7681 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG 7741 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA 7801 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAGA GTTGGTAGCT 7861 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 7921 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 7981 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 8041 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 8101 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 8161 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 8221 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAG //