// Acapella Assay Language / Image Analysis // 2021-09-27 11:55:54 +0200 // AcapellaVersion: 5.0.1.124082 #require AcapellaAssayLanguage #namespace AAL AAL::GetData() AAL::CalculateImage() AAL::CalculateImage() AAL::FindNuclei() AAL::FindCytoplasm() AAL::SelectCellRegion() AAL::CalculateIntensityProperties() AAL::CalculateIntensityProperties() AAL::SelectPopulation() AAL::SelectPopulation() AAL::CalculateIntensityProperties() AAL::SelectPopulation() AAL::SelectPopulation() AAL::ReturnResults() #begin:attachment(encoding="eventxml",name="EvaluationData") 1 1 1 builtin 1 Each GetData 1 1 Using Individual Planes, FFC None 1 eAELTi0pycxLL/4fkFOanplX/B8ARG8IMg== None 1 0 EACH 0 1 builtin Each CalculateImage_2 1 Replace me 1 1 Calculated Image NaNsCalculateImage_2 Output image: Calculated Image 0 sticky eAFzTsxJLs1JLElNUfDMTUxP/Q8AOewG9Q== eAHzS01PLMksS/0fmpeSmpaZl5ryHwBSSQjE Standard 0 eAFzTsxJLs1JLElNUfDMTUxP/Q8AOewG9Q== FeedBack.NaNsCalculateImage_2 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjAAAAAQAB 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 eAFjAAAAAQAB 1.0 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 eAFjAAAAAQAB 1.0 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 eAFjAAAAAQAB 1.0 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 eAFjAAAAAQAB 1.0 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZGRkBAAADgAF 1.0 eAFzL0qs/P+PAQQBIZ4FjQ== eAFzL0qs/P+PAQQBIZ4FjQ== eAFjYGBgBAAABQAC 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq fwB6yRDt eAFjYGBgAAAABAAB 1.0 eAELNw4y6Dn60N6757HMq+sP7NWX3I/wjrlkf8xG2O/nl8v2AAIAEFA= 1.0 eAE7aZJjfvpzgkODbYEa24xkh8rlz40UZbY63Pep/HOufbsDAOtTDuo= 1.0 eAHLn6Y+MVMi0uF/i/zmz+cjHT4oirlt/h7lwDnTVKZjYrMDAO4/Dgg= 1.0 eAFjYACBD/ZgCgsNAEFsBL0= 1.0 eAFjYACBB/ZgCgsNAD3sBH0= 1.0 eAFjYMAPAAAgAAE= 1.0 11 Regions eAH7/x8AAv8B/w== eAELSk35X5mak5Nf/h8AH7IFtg== eAELSk35X5mak5Nf/h8AH7IFtg== eAFjZAQAAAUAAw== 1.0 eAHzS01PLMksS/0fmpeSmpaZl5ryHwBSSQjE eAFjYAAAAAIAAQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFLTs0rSS36/48BABUgBH8= eAFzL0qs/P+PAQQBIZ4FjQ== 1 eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v4HAGoSGjo= eAELSk35X5mak5Nf/h8AH7IFtg== eAFjZAQAAAUAAw== 1.0 eAFLTs0rSS36/48BABUgBH8= Red center 1 colorset@rainbow Border colorset@rainbow Border Border 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAELSk35X5mak5Nf/h8AH7IFtg== eAFjZAQAAAUAAw== 1.0 Stencils Render 10.0 eAFLTs0rSS36/48BABUgBH8= 1 1 NaNs NaNs Images=AAL::GetValidImages().v_images; Set(Stencils=AAL::GetImageViewStencilsMultiList2(vec("EvaluationData.Procedures.CalculateImage_2.Outputs.Negativeillustration.dataitem","EvaluationData.Procedures.CalculateImage_2.Outputs.NaNillustration.dataitem"), vec("Negative","Undefined")).v_stencils) Standard EvaluationData.Procedures.CalculateImage_2.Outputs.Negativeillustration.dataitem 3.0 0 NaNsCalculateImage_2 1 By Formula Select a method 0 Complex s Method Method Discrete A+5*C Set to Local Average Set to Zero 0 CalculateImage_2 0 1 UI.InputParameters.CalculateImage_2.Method.title 1 eAFzqlRwyy/KLc1J/A8AF5oEsQ== By Formula eAFzqlRwyy/KLc1J/A8AF5oEsQ== A+B Type a formula in style A+B 0 Complex s sFormula Formula Numeric 0 CalculateImage_2 0 1 UI.InputParameters.CalculateImage_2.Method.sFormula 1 0 A+5*C Alexa 647 Select channel A 0 Complex s 1 1 sChannelA Channel A Discrete 1 CalculateImage_2 0 1 UI.InputParameters.CalculateImage_2.Method.sChannelA 1 eAHz8Hd19ggOUTA2NjYx+g8AHFUELQ== HOECHST 33342 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6D8Al1YJ+Q== Alexa 647 Select channel C 0 Complex s 1 1 sChannelC Channel C Discrete 1 CalculateImage_2 0 1 UI.InputParameters.CalculateImage_2.Method.sChannelC 1 eAFzdXcL+A8ABOsCIg== EGFP eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6D8Al1YJ+Q== Set to Zero Select channel C 0 Complex s sNegativeMethod Negative Values Discrete 0 CalculateImage_2 0 1 UI.InputParameters.CalculateImage_2.Method.More.sNegativeMethod 1 eAELTi1RKMlXiEotyv8PABvhBO8= Set to Zero eAELTi1RKMlXiEotyv8fnJGZVqLgmVeSmlecWZKZWvzfOzW14D8A+1EPHw== Set to Local Average Select channel C 0 Complex s sNanMethod Undefined Values Discrete 0 CalculateImage_2 0 1 UI.InputParameters.CalculateImage_2.Method.More.sNanMethod 1 eAELTi1RKMlX8MlPTsxRcCxLLUpMT/0PAFB2CBU= Set to Local Average eAELTi1RKMlX8MlPTsxRcCxLLUpMT/0fDBGMSi3K/w8AzUcNAw== Calculated Image Name of output Image 0 Complex s sOutputName Output Image Numeric 0 CalculateImage_2 0 1 UI.InputParameters.CalculateImage_2.sOutputName 1 0 Calculated Image 124082 122842 2 1 2 5.0.1.124082 0 1 eAFz/O/8HwAFigKD Output Image Calculated Image eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+AwB9YAoX eAEr8cxNTE/9DwALjANX eAH7DwABAAEA eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v4HAGoSGjo= eAFjAAAAAQAB 1.0 eAFzTsxJLs1JLEn1zE1MT403+g8AOzMHAg== eAFzTsxJLs1JLEn1zE1MT403+g8AOzMHAg== eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzTsxJLs1JLEn1zE1MT403+g8AOzMHAg== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA eAFzTsxJLs1JLElNUfDMTUxP/Q8AOewG9Q== eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv8Bwh0MPg== eAH7DwABAAEA eAFjBAAAAgAC 1.0 eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdaLjwdJgUWLHeCqUhyMDMBieilAgzJLUnP/AwBWeyMh eAFjBAAAAgAC 1.0 eAFzTsxJLs1JLElNUfDMTUxP/Q8AOewG9Q== eAFzTsxJLs1JLEn1zE1MT403+g8AOzMHAg== eAFjAgAAAwAD 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAGLj/dLTU8sySxL9S9LLcpJrEwtdk7MSS7NSSxJ9cxNTE+NN/ofH++XmIdbGgCR rBtD eAHzS01PLMksS83MySktLikCsvPz/vsl+qHwASuwEIM= eAHzS01PLMksS/XMySktLikCsvPz/vsl5qHwASgwEGM= eAGdzMENgCAMBdCNenAF5eAF3cD8SENIihhoXV+JZ08O8J67IAZN5ZigoLWWnYNV bjRCdhMozxmRt4EW09O0kefYwcVJxJrWF1PoOinn2/0I4b+vB/2PPkk= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PAQBLMwgA eAFzTsxJLs1JLEn1zE1MT403+v+PAQBLMwgA eAFjYgIAAAgABQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PAQBLMwgA eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== 1 Method MethodFolder InputParameters.CalculateImage_2.Method Method __BeforeFirstInput Method 1 1 0 MoreFolder InputParameters.CalculateImage_2.Method.More sChannelC 0 0 0 1 builtin Each CalculateImage 1 Replace me 1 1 Hoechst Mask NaNsCalculateImage Output image: Hoechst Mask 0 sticky eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzS01PLMksS/0fmpeSmpaZl5ryHwBSSQjE Standard 0 eAHzyE9NziguUfBNLM7+DwAifQV6 FeedBack.NaNsCalculateImage eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjAAAAAQAB 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PAQIBLrQGiw== eAFzL0qs/P+PAQIBLrQGiw== eAFjYGBkYAQAAAkAAw== 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjYAACAAAFAAE= 1.0 eAEBKADX/1czUjCMxeE/S4zjHOrX4D8npN9YS1zSP8Y8E0759NM/6iVy+rH80T+b +hWI 1.0 eAE7aZJjfvpzgkODbYEa24xkh8rlz40UZbY63Pep/HOufbsDAwjEbHUAAGVwEDs= 1.0 eAHLn6Y+MVMi0uF/i/zmz+cjHT4oirlt/h7lwDnTVKZjYrMDAxQAAF6ODgg= 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7/x8AAv8B/w== eAELSk35X5mak5Nf/h8AH7IFtg== eAELSk35X5mak5Nf/h8AH7IFtg== eAFjZAQAAAUAAw== 1.0 eAHzS01PLMksS/0fmpeSmpaZl5ryHwBSSQjE eAFjYAAAAAIAAQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFLTs0rSS36/48BABUgBH8= eAFzL0qs/P+PAQYBPcYHiQ== 1 eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxP1fMvLSko LSnWK4FwU4CqM0tSc/8DADwBGak= eAELSk35X5mak5Nf/h8AH7IFtg== eAFjZAQAAAUAAw== 1.0 eAFLTs0rSS36/48BABUgBH8= Red center 1 colorset@rainbow Border colorset@rainbow Border Border 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAELSk35X5mak5Nf/h8AH7IFtg== eAFjZAQAAAUAAw== 1.0 Stencils Render 10.0 eAFLTs0rSS36/48BABUgBH8= 1 1 NaNs NaNs Images=AAL::GetValidImages().v_images; Set(Stencils=AAL::GetImageViewStencilsMultiList2(vec("EvaluationData.Procedures.CalculateImage.Outputs.Negativeillustration.dataitem","EvaluationData.Procedures.CalculateImage.Outputs.NaNillustration.dataitem"), vec("Negative","Undefined")).v_stencils) Standard EvaluationData.Procedures.CalculateImage.Outputs.Negativeillustration.dataitem 3.0 0 NaNsCalculateImage 1 By Formula Select a method 0 Complex s Method Method Discrete iif(A>260, A, 0) Set to Local Average Set to Zero 0 CalculateImage 0 1 UI.InputParameters.CalculateImage.Method.title 1 eAFzqlRwyy/KLc1J/A8AF5oEsQ== By Formula eAFzqlRwyy/KLc1J/A8AF5oEsQ== A+B Type a formula in style A+B 0 Complex s sFormula Formula Numeric 0 CalculateImage 0 1 UI.InputParameters.CalculateImage.Method.sFormula 1 0 iif(A>260, A, 0) Alexa 647 Select channel A 0 Complex s 1 1 sChannelA Channel A Discrete 1 CalculateImage 0 1 UI.InputParameters.CalculateImage.Method.sChannelA 1 eAHz8Hd19ggOUTA2NjYx+g8AHFUELQ== HOECHST 33342 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq fwB6yRDt Set to Zero Select channel A 0 Complex s sNegativeMethod Negative Values Discrete 0 CalculateImage 0 1 UI.InputParameters.CalculateImage.Method.More.sNegativeMethod 1 eAELTi1RKMlXiEotyv8PABvhBO8= Set to Zero eAELTi1RKMlXiEotyv8fnJGZVqLgmVeSmlecWZKZWvzfOzW14D8A+1EPHw== Set to Local Average Select channel A 0 Complex s sNanMethod Undefined Values Discrete 0 CalculateImage 0 1 UI.InputParameters.CalculateImage.Method.More.sNanMethod 1 eAELTi1RKMlX8MlPTsxRcCxLLUpMT/0PAFB2CBU= Set to Local Average eAELTi1RKMlX8MlPTsxRcCxLLUpMT/0fDBGMSi3K/w8AzUcNAw== Calculated Image Name of output Image 0 Complex s sOutputName Output Image Numeric 0 CalculateImage 0 1 UI.InputParameters.CalculateImage.sOutputName 1 0 Hoechst Mask 124082 122842 2 1 2 5.0.1.124082 0 1 eAFz/A8AAYMBQQ== Output Image Hoechst Mask eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAGLj/dLTU8sySxL9S9LLcpJrEwtdk7MSS7NSSxJ9cxNTE+NN/ofH++XmIdXGr8R eA34DwB38DVj eAHzS01PLMksS83MySktLikCsvPz/vsl+qHw/zH8YwQAc7ESgA== eAHzS01PLMksS/XMySktLikCsvPz/vsl5qHw/zH8YwQAb7ESYA== eAGtzjEOgCAMQNEbdfAKyuCC3sA00BiSIgZarq/ExclFPcB/+aYiK0pI24CCMOfk yGumAj2yU0ahMeJKSweTyq5SwNLagkqBWYvkKwbf6iAUD/MCRPvN+nnt5p7GTmLP e28= eAFjYGBgAAAABAAB 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RgAQ3g9v eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RgAQ3g9v eAFjYmJmBgAAGwAL 1.0 eAFjYGBgAAAABAAB 1.0 eAFjYmZiBgAAHAAL 1.0 eAFjYGBgAAAABAAB 1.0 eAFjZGRkBAAADgAF 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RgAQ3g9v eAH7////fwAJ+gP9 eAH7////fwAJ+gP9 eAFjYGBgAAAABAAB 1.0 eAH7////fwAJ+gP9 1 Method MethodFolder InputParameters.CalculateImage.Method Method __BeforeFirstInput Method 1 1 0 MoreFolder InputParameters.CalculateImage.Method.More sChannelA 0 0 0 1 builtin Each FindNuclei 1 Replace me 1 1 Nuclei NucleiFindNuclei Population: Nuclei 0 sticky eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzK03OSS0t/g8ADvED3w== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.NucleiFindNuclei eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PITwjsyQVSP9jAABL3wiN eAFzL0qs/P+PAQIBLrQGiw== eAFjYGBkYAAAAAgAAg== 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjYGBkYAAAAAgAAg== 1.0 eAEBKADX/1czUjCMxeE/S4zjHOrX4D8npN9YS1zSP9Dxm7lCytM/6iVy+rH80T+p shZZ 1.0 eAE7aZJjfvpzgkODbYEa24xkh8rlz40UZbY6BH+uTjikuN2BAQRitjoAAFm3D0o= 1.0 eAHLn6Y+MVMi0uF/i/zmz+cjHT4oirlt/h7lkJWq8OeNYbMDAxQAAGxcDwQ= 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7DwABAAEA eAFLzs/JLypOLXEoSszMS8ov/w8AP9wHnQ== eAFLzs/JLypOLXEoSszMS8ov/w8AP9wHnQ== eAFjBAAAAgAC 1.0 eAHzK03OSS0t/g8ADvED3w== eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAFLyi9KSS36DwAMKwN+ eAFzL0qs/P+P4R9DeEZmSSqYBQBe6QmL 1 eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFLzs/JLypOLXEoSszMS8ov/w8AP9wHnQ== eAFjBAAAAgAC 1.0 eAFLyi9KSS36DwAMKwN+ colorset@rainbow border 1 colorset@rainbow Border Border 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov/6/s5mZpbOD4HwCFZAoo eAFjZAQAAAUAAw== 1.0 Stencils Render 10.0 eAFLyi9KSS36X5yfk5nyHwAqrgaY 1 1 Nuclei Nuclei Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.FindNuclei.Outputs.Nuclei.dataitem 3.0 1 Initial Mask InitialMaskFindNuclei Population: Initial Mask 0 sticky eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzzMssyUzMUfBNLM7+DwAilQV2 Standard 0 eAELKMovSC0qyUwtVvDMyyzJTMxR8E0szv4PAHVgCcM= FeedBack.InitialMaskFindNuclei eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 1 SingleDefaultOverLay WeightedAverage script 0 1 eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PAQIBLrQGiw== eAFjYGBgYAQAAAYAAg== 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== 1 eAHzzMssyUzMUfBNLM7+DwAilQV2 eAFjBAAAAgAC 1.0 eAErSk35DwAEwgI7 eAFLyk+p/A8ABsgCrg== eAErSk35DwAEwgI7 eAENyksOgDAIBcAbcQo1ceHnCqRlQaTUlIfXV2c988OWDO0+MZjO0YvUHBK0qNc9 i4nSkbgTQasrlG3juKj+XSHt/QBVGxoY eAErSk35DwAEwgI7 eAFjBAAAAgAC 1.0 eAFLyk+p/A8ABsgCrg== red body 1 colorset@rainbow Body Body 0 1 1 InitialMask InitialMask Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.FindNuclei.Outputs.InitialMask.dataitem 3.0 1 Low Contrast Objects LowContrastFindNuclei Population: Low Contrast Objects 0 sticky eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzyS9XcM7PKylKLC5R8E/KSk0uKf4PAFWWCIo= Standard 0 eAELKMovSC0qyUwtVvDJL1dwzs8rKUosLlHwT8pKTS4p/g8AyskM1w== FeedBack.LowContrastFindNuclei eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 1 SingleDefaultOverLay WeightedAverage script 0 1 eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PAQIBLrQGiw== eAFjYGBgYAQAAAYAAg== 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== 1 eAHzyS9XcM7PKylKLC5R8E/KSk0uKf4PAFWWCIo= eAFjBAAAAgAC 1.0 eAErz8gsSf0PAAmhAyE= eAFLyi9KSS36DwAMKwN+ eAErz8gsSf0PAAmhAyE= eAENy0sOgCAMBcAb9RJ+Vka8QoUuSpAaeNXjK9tJZnm4OEOtzgymo1mU5E06rVrT 7rGIUnDcjk6bvZNVNO4IZ5Y4KI2lkOv7ARZDHQw= eAErz8gsSf0PAAmhAyE= eAFjBAAAAgAC 1.0 eAFLyi9KSS36DwAMKwN+ white border 1 colorset@rainbow Body Body 0 1 1 LowContrast LowContrast Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.FindNuclei.Outputs.LowContrastObjects.dataitem 3.0 0 NucleiFindNuclei 1 HOECHST 33342 Select channel 0 Complex s 1 0 NucleiDetectionChannel_string Channel Discrete 1 eAHzK03OSc38DwALhgNg FindNuclei CalculateImage 0 0 UI.InputParameters.FindNuclei.NucleiDetectionChannel_string 1 eAHzyE9NziguUfBNLM7+DwAifQV6 Hoechst Mask eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== None Select population for ROI 0 Complex s WholeCells_string ROI Discrete 0 FindNuclei 0 1 UI.InputParameters.FindNuclei.WholeCells_string 1 eAHzy89L/Q8ABloCkA== None eAHzy89L/Q8ABloCkA== Select ROI region 1 Complex s 1 Region Discrete 0 FindNuclei 0 1 UI.InputParameters.FindNuclei.Region 1 eAH7DwABAAEA eAH7DwABAAEA B Select method 0 Complex s NucleiDetectionAlgorithm Method Discrete 0 FindNuclei 0 0 UI.InputParameters.FindNuclei.AdditionalParameters.title 1 eAFz+g8AAYUBQg== B eAFz/O/03/m/738AFTIFEA== 0.4 Threshold tuning for the initial thresholding of nuclei. Range: 0..1.0. Low values shrink the mask and the high ones expand the mask. Adjust by illustration InitialMask. 0 Simple d ThresholdAdjustment Common Threshold 1.0 0.0 Numeric 1 AAL::GoodnessProc_CommonThreshold() eAHzzMssyUzM8U0sznbLzEvxK03OSc38D6GQBAA7mRB3 0 FindNuclei 0 0 0.05 UI.InputParameters.FindNuclei.AdditionalParameters.ThresholdAdjustment 1 1 0 eAGbNRMEdtrPAtMn7Y3B4DKUf9OeAQweQMUf26eBwTOo/Ev7s2dA4A1U3Qd7AK9V IwA= 1.0 0.1 30 Nuclei: Minimum allowed area for nuclei, objects with area less than the limit are removed. Too small values of the parameter usually introduce errors. Unit: image pixel. Adjust by illustration Nuclei. 0 Simple d MinimumNuclearArea Minimum Area 500000000 0 Numeric 0 FindNuclei > 0 0 1 UI.InputParameters.FindNuclei.AdditionalParameters.MinimumNuclearArea 1 0 µm² 15 eAFjZeBiEGGQYzBiCGCoYDjBMIFRgRkAFH4CuA== 1.0 3 7.0 Nuclei: Controls splitting of stuck nuclei. Range: 1..100. With the lower values more objects are split. Adjust by illustration Nuclei. Parameter has only limited control other the splitting process. Adjust by illustration Nuclei. 0 Simple d NuclearSplittingAdjustment Splitting Coefficient 100.0 1.0 Numeric 0 FindNuclei 0 0 0.5 UI.InputParameters.FindNuclei.AdditionalParameters.More.NuclearSplittingAdjustment 1 1 100 eAFjYmZl5+KXBAAAsQBE 1.0 1.0 0.4 Nuclei: Controls the size (border position) of nuclei. Range: 0..~1.0. With the higher values nuclei appear smaller and with the lower values larger. Adjust by illustration Nuclei. 0 Simple d IndividualThresholdAdjustment Individual Threshold 1.0 0.0 Numeric 1 OptimizeITA() NucleiDetectionData.NucleIIllustrations.Nuclei Nuclei: Controls the size (border position) of nuclei. Range: 0..~1.0. With the higher values nuclei appear smaller and with the lower values larger. Adjust by illustration Nuclei. 0 FindNuclei 0 0 0.05 UI.InputParameters.FindNuclei.AdditionalParameters.More.IndividualThresholdAdjustment 1 1 0.3 eAGbNRMEdtrPAtMn7Y3B4DKUf9OeAQweQMUf26eBwTOo/Ev7s2dA4A1U3Qd7AK9V IwA= 1.0 0.1 0.1 Nuclei: Minimum allowed contrast for nuclei, objects with contrast less than the limit are removed. Range: 0..1.0. Adjust by illustration LowContrastObjects and Nuclei. 0 Simple d MinimumNuclearContrast Minimum Contrast 1.0 -1.0 Numeric eAHzyS93zs8rKUosLnHLzEvxK03OSc38D6GQBABAjxCh 0 FindNuclei > 0 0 0.05 UI.InputParameters.FindNuclei.AdditionalParameters.More.MinimumNuclearContrast 1 1 0.11 eAFjYACBB/vBFJCYNRMEVtpD6J32xmBwGMo/CaVvQsUf2wMArp0YhA== 1.0 0.1 13.0 Expected diameter. Smaller values yield more fragmented and smaller objects. Range: 2..40. 0 Complex d NucDiam Diameter 40.0 2.0 Numeric 1 Set(NucDiam=NucleiDetectionData.EstimatedDiam) NucleiDetectionData.Nuclei Expected diameter. Smaller values yield more fragmented and smaller objects. Range: 2..40. 0 FindNuclei 1 0 2.0 UI.InputParameters.FindNuclei.AdditionalParameters.NucDiam 0 0 µm 13 4.0 0.4 Controls splitting of stuck nuclei. Range: 0..1.0. At the value 0.0, splitting is switched off. Higher values return increasingly more splitting events. 0 Complex d SplitTuner Splitting Sensitivity 1.0 0.0 Numeric 0 FindNuclei 1 0 0.05 UI.InputParameters.FindNuclei.AdditionalParameters.SplitTuner 0 1 0.4 0.1 0.4 This is a relative threshold determining how dark objects can be detected. Range: 0..1.0. With smaller values, darker objects are detected. 0 Complex d DynRanThr Common Threshold 1.0 0.0 Numeric 0 FindNuclei 1 0 0.05 UI.InputParameters.FindNuclei.AdditionalParameters.DynRanThr 0 1 0.4 0.1 Nuclei Name of the output population. 0 Complex s OutputName Output Population Numeric 1 Nuclei Nuclei 1 0 FindNuclei 0 1 UI.InputParameters.FindNuclei.OutputName 1 0 Nuclei 124082 122842 7 1 eAHzK03OSc38DwALhgNg 7 5.0.1.124082 0 Output Population Nuclei eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAF9zEEKgCAQRuGreIBWXaFVUHaE4U+GsCaFHI1Ob5to6fZ98Igsb1BfeCl8CR5O A8RlgfJ4YmPqK5FFaHJ70RxUm52wr2Pw6iFmRjo688Up3sbFoBeSmrju7DT9+ALy 2ExY eAHzS01PLMksS83MySktLikCsvPz/vsl+qHw/zH8Y/QrTc5JzfzvmZdZkpmY45tY nP3fJ7/cOT8PqKm4xD8pKzW5pPg/AHepI30= eAHzS01PLMksS/XMySktLikCsvPz/vsl5qHw/zH8Y/QrTc5JzfzvmZdZkpmYo+Cb WJz93ye/XME5Pw+orbhEwT8pKzW5pPg/AMoMI70= eAGtkUsOwjAMRG+UBVcoRapUUm6ATGJVBjdBid0eH6Ii0U0X5bOcxXsazdQjsIJQ DHsQMKcUHXpNmE0F7JRBsBmgx/POdCp3lWws9gUYkZg1S5ph4wtNgsOj/kII9jfX n6stus+LHSh4q46RFssrblhoBW4CCQEfId+2bLxiaONUxVB+ytJdrujKg+8qT6bs yrI= eAFjYGBgYGRgAAAACgAC 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5j+MQEAK4MW Tw== eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5j+MQEAK4MW Tw== eAFjYmJmZmFhAQAAVAAX 1.0 eAFjYAADAAAHAAE= 1.0 eAFjYmZiZmRmAQAASgAT 1.0 eAFjYAADAAAHAAE= 1.0 eAFjZAQDAAAjAAg= 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5j+MQEAK4MW Tw== eAH7////f7/8vFQgBQA0VQiK eAH7/x8MABvrBvo= eAFjYAADAAAHAAE= 1.0 eAH7////f4/81OSM4hIF38TibCAXAHYsC3Q= eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA 1 AdditionalParameters MethodFolder InputParameters.FindNuclei.AdditionalParameters NucleiDetectionAlgorithm Region AdditionalParameters 0 0 0 1 builtin Each FindCytoplasm 1 Replace me 1 1 Nuclei CellsFindCytoplasm Population: Nuclei 0 sticky eAFzTsxJLs1JLElNUfDMTUxP/Q8AOewG9Q== eAFzTs3J+Q8ABgMCgA== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.CellsFindCytoplasm eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PQdnQxNXJ1QnI+scAAFDUCCI= eAFzL0qs/P+PAQIBLrQGiw== eAFjYGBkZAAAAAoAAw== 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjZGBkZAQAABAABQ== 1.0 eAEBKADX/1czUjCMxeE/S4zjHOrX4D8npN9YS1zSP8Y8E0759NM/6iVy+rH80T+b +hWI 1.0 eAE7aZJjfvpzgkODbYEa24xkh8rlz40UZbY63Pep/HOufbsDAwjEbHUAAGVwEDs= 1.0 eAHLn6Y+MVMi0uF/i/zmz+cjHT4oirlt/h7lwDnTVKZjYrMDAxQAAF6ODgg= 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7////fwAJ+gP9 eAFLzs/JLypOLXEoSszMS8ov//+PAQQBeXIKlw== eAFLzs/JLypOLXEoSszMS8ov//+PAQQBeXIKlw== eAFjZGRgAAAACwAD 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJx7n8A868PPw== eAFjYGBgAAAABAAB 1.0 eAFjYGBgAAAABAAB 1.0 eAFzyi9KSS36/48BBAErZwZY eAFzL0qs/P+P4R+DsqGJq5OrE5gNAGXaCSA= 1 eAENyzEKgDAMAMAf5RNVV90cJdiAgbQpTVLw9drbbx0ogc5aF3SEo+tNOToZbFxz el2boBXYw1u4wfmoUCIRu1gkzPvMgyDPzU7l+wETViCX eAFLzs/JLypOLXEoSszMS8ov//+PAQQBeXIKlw== eAFjYGRgAAAABwAC 1.0 eAFzyi9KSS36/48BBAErZwZY colorset@rainbow Border 1 colorset@rainbow Border Border 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQSV3dwsjQ0c/wMA1soNIg== eAFjZGRgYAQAAA8ABA== 1.0 Stencils Render 10.0 eAFzyi9KSS36/48BBIvzczJT/gMAWwYJcg== 1 1 Cells Cells Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.FindCytoplasm.Outputs.WholeCells_illustrative.dataitem 3.0 0 CellsFindCytoplasm 1 Alexa 647 Select channel 0 Complex s 1 0 CytoplasmDetectionChannel_string Channel Discrete 1 FindCytoplasm CalculateImage_2 0 0 UI.InputParameters.FindCytoplasm.CytoplasmDetectionChannel_string 1 eAFzTsxJLs1JLElNUfDMTUxP/Q8AOewG9Q== Calculated Image eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== Nuclei Select nuclei population 0 Complex s Nuclei_string Nuclei Discrete eAHzK03OSc38DwALhgNg eAHzK03OSc38DwALhgNg eAHzK03OSc38DwALhgNg eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdaD clOAKjNLUnP/AwDZaxgi eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 FindCytoplasm 0 1 UI.InputParameters.FindCytoplasm.Nuclei_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc38DwALhgNg A Select a method 0 Complex s Method Method Discrete 0 FindCytoplasm 0 0 UI.InputParameters.FindCytoplasm.Method.title 1 eAFz+g8AAYUBQg== B eAFz/O/03/m/y3/X/27/AS7YB5A= Alexa 647 Select membrane stained channel 0 Complex s 1 1 MembraneChannel_string Membrane Channel Discrete 1 FindCytoplasm 1 0 UI.InputParameters.FindCytoplasm.Method.MembraneChannel_string 0 eAFzzEmtSFQwMzH/DwASlwOs Alexa 647 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== 0.45 Controls common threshold calculation for cytoplasm detection 0 Simple d CytoplasmThresholdAdjustment Common Threshold 1.0 0.0 Numeric 0 FindCytoplasm 0 0 0.05 UI.InputParameters.FindCytoplasm.Method.CytoplasmThresholdAdjustment 1 1 0.25 0.1 0.15 Controls individual threshold calculation, as result cytoplasm/cell regions can appear larger or smaller 0 Simple d CytoplasmIndividualThresholdAdjustment Individual Threshold 1.0 0.0 Numeric 1 GoodnessProc_CytoplasmIndividualThreshold() 0 FindCytoplasm 0 0 0.05 UI.InputParameters.FindCytoplasm.Method.CytoplasmIndividualThresholdAdjustment 1 1 0.39 eAGbNRMEVtrPAtM77Y3B4DCUfxLKvwzl37RnAIMHUPHH9mlg8Awq/9L+7BkQeANV 98EeABv8KbM= 1.0 0.05 Select ROI region 0 Simple s sROIRegion Restrictive Region Discrete 0 FindCytoplasm 0 1 UI.InputParameters.FindCytoplasm.Method.sROIRegion 1 eAH7DwABAAEA eAH7DwABAAEA 0 Use ROI region 1 Complex b yRoiOnOff Numeric 0 FindCytoplasm 0 1 UI.InputParameters.FindCytoplasm.Method.sROIRegion.qualifier 1 0 0 MultiNucleated Cells Name of the output population 0 Complex s OutputName Output Population Numeric 1 MultiNucleated Cells MultiNucleated Cells 1 0 FindCytoplasm 1 1 UI.InputParameters.FindCytoplasm.OutputName 0 0 MultiNucleated Cells 124082 122842 5 1 eAHzLc0pyfQrTc5JTSxJTVFwTs3JKf4PAFqwCLM= 5 5.0.1.124082 0 Output Regions Cell, Cytoplasm, Membrane eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAF9j0EKwjAQRa+SA7jyCgWhoPEIwzcOZXSaSGdS6emjQnGZ7X+PB58o8gSXla8r L4qNbYCmqnAeZ0xMx0YUkbu4n+gGWqxJWdqYxQUaLrDnIezjubxDKtkXmIdye3By +8PdOUm+D5uXl8JmEtVqX//3qH0AF2Bbeg== eAFVjDEOgCAMAPVtTCaKo6OppNFqA4mt+Hsqm7rdDXceV1DKSMyX6Fk5RfPgf16a 0vorMJJ1kZSAB5DD+nS7FGskOi47BhWbtsTokPmL8zvLaA9cYjLm eAFVyzEOgCAMQFH1JNyFiURxdK6kUbQpiS16e6qr23/Dj7iB5hsDURW9vi5sEfjn 1rU+1kSYLXDWDOQmkNPG8jhf+NtE3bwemFSsDcteCD0Sib3B/Cmu eAGtkTEOwjAMRW+UgSuEIiFBy8ZYmdQqBjepGruI00MEElk6FOjo4T89/1+MwApC wa9BwByG4LDRAaOxwE4ZBLcdtFivTKXSq0RTYpsCIxKzRhleYdOkNAl2j+IHIJT/ sRZWy7jvxTbkm1IdI2XK+5zR0ER460kIeA/xOqfjCcIu3Gzwaaco1emCLi04U8Xe JfQMsfu8cjwHRovMizDqvPqIWeoJ2i4GZg== eAFjYAADRgYAAAsAAg== 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5hAECTgXFmS X5CTWJz7HwDKFx2L eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5j+MYG4zpUl +QU5icW5//8xAwDIkB2M eAFjYmJmZmFhYWUFAACRACE= 1.0 eAFjYIACAAAJAAE= 1.0 eAFjYmZiZmRmYWQCAAB0ABY= 1.0 eAFjYGBgYAViBgAAIgAG 1.0 eAFjZAQDBkYAADQACQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5hAECTgXFmS X5CTWJz7HwDKFx2L eAH7////f7/8vFQg9Y/hPwBR7AqH eAH7/x8KACzcCPg= eAFjYIACAAAJAAE= 1.0 eAH7////f4/81OSM4hIF38TibCD3H8N/AJyBDXE= eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA 1 Method MethodFolder InputParameters.FindCytoplasm.Method Method Nuclei_string Method 0 1 0 1 builtin Each SelectCellRegion 1 Replace me 1 1 Nuclei CellRegionSelectCellRegion The created region Cytoplasm Region. 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.CellRegionSelectCellRegion eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PAQIBLrQGiw== eAFzL0qs/P+PAQIBLrQGiw== eAFjYAACAAAFAAE= 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjYAACAAAFAAE= 1.0 eAEBKADX/1czUjCMxeE/S4zjHOrX4D8npN9YS1zSP8Y8E0759NM/6iVy+rH80T+b +hWI 1.0 eAE7aZJjfvpzgkODbYEa24xkh8rlz40UZbY63Pep/HOufbsDAwjEbHUAAGVwEDs= 1.0 eAHLn6Y+MVMi0uF/i/zmz+cjHT4oirlt/h7lwDnTVKZjYrMDAxQAAF6ODgg= 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7/x8IAA72BPw= eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGBgYAQAAAYAAg== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFjZAACAAAKAAI= 1.0 eAFjYAACAAAFAAE= 1.0 eAELzs/JTPnvlJ9S+f8fIwgCAEsACIU= eAFzL0qs/P+PAQYBPcYHiQ== 1 eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1gtOzUlNLnFOzckJSk0Hyuv5l5YU lJYU64Vn5OekgsSL9VKAejJLUnP/AwDckxwX eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGBgYAQAAAYAAg== 1.0 eAFzyk+p/P+PAQIBLnAGhg== colorset@rainbow Body 1 colorset@rainbow Body Body 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFjYGBgYGQEAAAJAAM= 1.0 Stencils Render 10.0 eAELzs/JTPnvlJ9S+f8fIwgWgwUAh60Lnw== 1 1 CellRegion CellRegion Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectCellRegion.Outputs.WholeCells.dataitem 3.0 0 CellRegionSelectCellRegion 1 Nuclei Select population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAENyzEOgDAIAMAf8Ymqq26diZBIQktTwMTXq7ffeqMmhlhfMBCOaSdTTnbYpFN5 woaiN9gzRoZDvUy5sKoD/UGC2/sBj7cbEA== eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xLca4syS/ISSzO/Q8AKaUGPQ== eAFjBQAABgAG 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 SelectCellRegion 0 1 UI.InputParameters.SelectCellRegion.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc38DwALhgNg Nucleus Select nucleus region, i.e. center region 0 Complex s CenterRegion Center Region Discrete 1 SelectCellRegion 1 1 eAHzK03OSS0t/g8ADvED3w== eAELSk3PzM/7DwALcgNk eAHzK03OSS0t/g8ADvED3w== eAHzK03OSS0t/g8ADvED3w== eAFzy8xL8StNzknN/A8AGaQE4Q== eAEz+Q8AAWkBNA== eAHzK03OSS0t/g8ADvED3w== eAHzK03OSS0t/g8ADvED3w== eAH7DwABAAEA eAHzK03OSS0t/g8ADvED3w== eAH7DwABAAEA eAHzyE9NziguUfBNLM7+DwAifQV6 eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjYGBgAAAABAAB 1.0 5.0 UI.InputParameters.SelectCellRegion.CenterRegion 0 eAHzK03OSS0t/g8ADvED3w== Nucleus eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJx7n8A868PPw== Cell Select cell region 0 Complex s RestrictiveRegion Restrictive Region Discrete 0 SelectCellRegion 1 0 UI.InputParameters.SelectCellRegion.RestrictiveRegion 0 eAFzTs3J+Q8ABgMCgA== Cell eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJx7n+/fCAXAEZOEc4= Resize Region [%] Select a method 0 Complex s Method Method Discrete % 0 SelectCellRegion 0 1 UI.InputParameters.SelectCellRegion.Method.title 1 eAELSi3OrEpVCEpNz8zPU4hWjf0PAD9fBvM= Resize Region [%] eAELSi3OrEpVCEpNz8zPU4hWjf0fhCpyaGuufkFF7H8ASvoQuw== Cytoplasm Region Select region type. Supported types are: Cell Region, Nucleus Region, Cytoplasm Region, Membarne Region and Ring Region. Please note also that region shape depends on unit type: relative unit options might result qualitively different regions compared to absolute unit (pixel/µm) ones. 0 eAFzTs3JUQhKTc/Mz/vvV5qck1paDOM6V5bkF+QkFufCBHxTc5OKEvNSYfygzLx0 GBsAPlMeYg== Complex s RegionType Region Type Discrete Cytoplasm Region Cytoplasm Region 1 SelectCellRegion 0 1 UI.InputParameters.SelectCellRegion.Method.RegionType 1 eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== Cytoplasm Region eAFzTs3JUQhKTc/Mz/vvV5qck1paDOM6V5bkF+QkFufCBHxTc5OKEvNSYfygzLx0 GBsAPlMeYg== % Unit 0 Complex s UnitType UnitType Numeric 1 0 SelectCellRegion 1 0 UI.InputParameters.SelectCellRegion.Method.UnitType 0 0 % 5 Outer border shift 0 Complex d OuterBorderShift Outer Border 100 -200 Numeric -40 1 SelectCellRegion 0 0 1 UI.InputParameters.SelectCellRegion.Method.OuterBorderShift 1 0 % -40 eAE7d+PRm28MXCJyGkY2bgFRKQBGEAaR 1.0 5 % Unit 0 Complex s UnitType2 UnitType Discrete 0 SelectCellRegion 1 1 UI.InputParameters.SelectCellRegion.Method.UnitType2 0 eAFT/Q8AAUsBJQ== % eAFT/Q8AAUsBJQ== 45 Inner border shift 0 Complex d InnerBorderShift Inner Border 100 -200 Numeric 100 1 SelectCellRegion 0 0 1 UI.InputParameters.SelectCellRegion.Method.InnerBorderShift 1 0 % 100 eAFjYAABlwNgisEESkN4DAwmDhCWC5T2g9IhUDrSAQCV1gSs 1.0 5 Cytoplasm Region Name of the created region 0 Complex s RegionName Output Region Numeric 0 SelectCellRegion 0 1 UI.InputParameters.SelectCellRegion.RegionName 1 0 Cytoplasm Region 124082 122842 3 1 3 5.0.1.124082 0 Output Region Cytoplasm Region eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAF9j0EKwjAQRa+SA7jyCgWhoPEIwzcOZXSaSGdS6elTheqy2/8eDz5R5AEuM19n nhQLWwdNVeHcjxiYjo0oIu/i/cRuoMWalKX1WVyg4QJ7HsI2nss7pJJ9gnkotwcn tz/cnJPke7d4eSlsJFGt9vG/j37pFaNTXtk= eAFVjLEOgCAMBfXbmEwUR0dTSaPVBhJb8O9BNnG7l9w7izsoJSTmKHpXDr5YsL+d u9zb6BipDJ6UgCeQq4zhMcHXk+i8nehUynIERoPMLa5fLGGrvKadN9c= eAFVyzEOgCAMQFH1JNyFiURxdEbSKNqUxBa9PRVHt/+G72ELkm5wiIXlap1JfaCf a1d7XyJCUkdJUkAzBT51zI+xmdrGYub1gCisdVj2jGAB8cMLF2Uqrg== eAGtkjEOwjAMRW+UgSuEIiFBi2BgrExqlYCbVI1dxOkhgESXCgXo6OG/PPsn64EE 2Ho3Bwa16bzBSjoMSgMZIWBcNlBjOVOFcCscVI51DPRoiSRw9wyrKqYtY3PLfgBC /h9rYrUB973YwroqF0NoB8prTLjQSHjpLFugNYRzyo1HCCt/0d7FngIXhxOa2GCi ir6ybwlC815lf/SEGokmYZRD6z2mSO2Qov/j/S3W8ct+9LoDfPEifA== eAFjYIACRgAACwAC 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5hAECTgXFmS X5CTWJwLFAMABzoeiw== eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5j+MYG4zpUl +QU5icW5//8xB6fmpCaXOKfm5ASlpmfm5/0HAPqIJM8= eAFjYmJmZmFhYWVlAwAAuAAn 1.0 eAFjYIABAAAKAAE= 1.0 eAFjYmZiZmRmYWRiBAAAiwAX 1.0 eAFjYGBgYGVgYGNgAAAAOgAM 1.0 eAFjZAQDBkYGAAA9AAk= 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5hAECTgXFmS X5CTWJz7/x8zAAc7How= eAH7////f7/8vFQg9Y8BiABo9guF eAH7/x8GADbTCfc= eAFjYIABAAAKAAE= 1.0 eAH7////f4/81OSM4hIF38TibCD3HwMQAQC5Xw5v eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA 1 Method MethodFolder InputParameters.SelectCellRegion.Method Method RestrictiveRegion Method 1 1 0 1 builtin Each CalculateIntensityProperties_3 1 Replace me 1 1 Nuclei PopulationCalculateIntensityProperties_3 Population: Nuclei 0 sticky eAHz8Hd19ggOUTA2NjYx+g8AHFUELQ== eAHzK03OSS0t/g8ADvED3w== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationCalculateIntensityProperties_3 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PAQIBLrQGiw== eAFzL0qs/P+PAQIBLrQGiw== eAFjYGBkYAAAAAgAAg== 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjYAACAAAFAAE= 1.0 eAEBKADX/1czUjCMxeE/S4zjHOrX4D8npN9YS1zSP8Y8E0759NM/6iVy+rH80T+b +hWI 1.0 eAE7aZJjfvpzgkODbYEa24xkh8rlz40UZbY63Pep/HOufbsDAwjEbHUAAGVwEDs= 1.0 eAHLn6Y+MVMi0uF/i/zmz+cjHT4oirlt/h7lwDnTVKZjYrMDAxQAAF6ODgg= 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7/x8IAA72BPw= eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjZAACAAAKAAI= 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFjYAACAAAFAAE= 1.0 eAFjYAACAAAFAAE= 1.0 eAFzyi9KSS36/48BAgE6EwdW eAFzL0qs/P+PAQYBPcYHiQ== 1 eAENzDEKgDAMBdAbZfEI6uCkm6ME/WAhtqX5ETy9Oj94460WylTyoFRZWtlxRINL r7aHKTFlInvi82NFY4JvnczBGnRZz2LoYeZy/EMirvcDmIoh5w== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAFzyi9KSS36/48BAgE6EwdW colorset@rainbow Border 1 colorset@rainbow Border Border 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFjZAACRgAADQAD 1.0 Stencils Render 10.0 eAFzyi9KSS36/48BAovzczJT/gMAb6YKcA== 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.CalculateIntensityProperties_3.Outputs.WholeCells.dataitem 3.0 0 PopulationCalculateIntensityProperties_3 1 Alexa 647 Signal channel 0 Complex s 1 1 channel_string Channel Discrete 1 CalculateIntensityProperties_3 0 1 UI.InputParameters.CalculateIntensityProperties_3.channel_string 1 eAHz8Hd19ggOUTA2NjYx+g8AHFUELQ== HOECHST 33342 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== Nuclei Select population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1gtOzUlNLnFOzckJSk0Hyuv5l5YU lJYU64Vn5OekgsSL9VKAejJLUnP/AwDckxwX eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAELTs1JTS5xTs3JCUpNz8zP+w8AO58HRA== eAFjAwAABwAH 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAFzy8xLca4syS/ISSzO/Q8AKaUGPQ== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 CalculateIntensityProperties_3 0 1 UI.InputParameters.CalculateIntensityProperties_3.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc38DwALhgNg Cell Select region which should be characterized. 0 Complex s Region Region Discrete 1 CalculateIntensityProperties_3 0 1 eAHzK03OSS0t/g8ADvED3w== eAELSk3PzM/7DwALcgNk eAHzK03OSS0t/g8ADvED3w== eAHzK03OSS0t/g8ADvED3w== eAFzy8xL8StNzknN/A8AGaQE4Q== eAEz+Q8AAWkBNA== eAHzK03OSS0t/g8ADvED3w== eAHzK03OSS0t/g8ADvED3w== eAH7DwABAAEA eAHzK03OSS0t/g8ADvED3w== eAH7DwABAAEA eAHzyE9NziguUfBNLM7+DwAifQV6 eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjYGBgAAAABAAB 1.0 5.0 UI.InputParameters.CalculateIntensityProperties_3.Region 1 eAHzK03OSS0t/g8ADvED3w== Nucleus eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== Standard Select a method 0 Complex s Method Method Discrete 0 0 0 1 0 0 50 0 0 0 CalculateIntensityProperties_3 0 1 UI.InputParameters.CalculateIntensityProperties_3.Method.title 1 eAELLknMS0ksSvkPABJkBDE= Standard eAELLknMS0ksSvkPABJkBDE= 1 Turn the Mean Intensity calculation ON/OFF 0 Complex b Mean_ONOFF Mean 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.Mean_ONOFF 1 0 1 1 0 Turn the Standard Deviation (StdDev) calculation ON/OFF 0 Complex b Stddev_ONOFF Standard Deviation 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.Stddev_ONOFF 1 0 0 1 0 Turn the Coefficient of Variance (CV) calculation ON/OFF 0 Complex b CV_ONOFF Coefficient of Variance 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.CV_ONOFF 1 0 0 1 0 Turn the Meadian Intensity calculation ON/OFF 0 Complex b Median_ONOFF Median 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.More.Median_ONOFF 1 0 0 1 0 Turn the summarized/integral intensity calculation ON/OFF 0 Complex b Sum_ONOFF Sum 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.More.Sum_ONOFF 1 0 0 1 0 Turn the Maximum Intensity calculation ON/OFF 0 Complex b Max_ONOFF Maximum 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.More.Max_ONOFF 1 0 0 1 0 Turn the Minimum Intensity calculation ON/OFF 0 Complex b Min_ONOFF Minimum 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.More.Min_ONOFF 1 0 0 1 50 For the quantile calculation all pixels are sorted according to their intensity. The quantile is the intensity of that pixel, which is brighter than the QuantileFraction number of pixels. The statistic "median" is the shorthand for "quantile" at 0.5. 0 Complex d QuantileFraction Quantile Fraction 100 0 Numeric 0 CalculateIntensityProperties_3 0 1 5 UI.InputParameters.CalculateIntensityProperties_3.Method.More.QuantileFraction 1 1 % 50 10 0 Turn the Quantile Intensity calculation ON/OFF 0 Complex b Quantile_ONOFF Numeric 0 CalculateIntensityProperties_3 0 1 UI.InputParameters.CalculateIntensityProperties_3.Method.More.QuantileFraction.qualifier 1 0 0 0 Turn the Contrast calculation ON/OFF 0 Complex b Contrast_ONOFF Contrast 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.More.Contrast_ONOFF 1 0 0 1 Intensity Nucleus HOECHST 33342 Prefix for property names. 0 Complex s SuffixForPropertyNames Output Properties Numeric 1 Intensity Nucleus HOECHST 33342 Intensity Nucleus HOECHST 33342 0 0 CalculateIntensityProperties_3 0 1 UI.InputParameters.CalculateIntensityProperties_3.SuffixForPropertyNames 1 0 Intensity Nucleus HOECHST 33342 124082 124082 3 1 3 5.0.1.124082 0 Output Property Intensity Nucleus HOECHST 33342 Mean eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= 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InputParameters.CalculateIntensityProperties_3.Method.More CV_ONOFF 0 0 0 1 builtin Each CalculateIntensityProperties_2 1 Replace me 1 1 Nuclei PopulationCalculateIntensityProperties_2 Population: Nuclei 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationCalculateIntensityProperties_2 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 MaxValue:Plain eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 MaxValue eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Plain eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjYAQCAAAPAAU= 1.0 eAFTdnN2NTN0++9elFj5/x8jCAIAR1EILQ== eAFzL0qs/P+PAQIBLrQGiw== eAFjZAACAAAKAAI= 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjZGBgZAAAAAwAAw== 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjOsQuX/Mhx4EBBBqSHEAUw7t1EHrnRgj9Zp0DAMG6CYU= 1.0 eAF7/vdYoYx6pMP/FvnNn89HOnxQFHPb/D3KgXOmqUzHxGYHBigAAIsyDyg= 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7/x8IAA72BPw= eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGBgYAQAAAYAAg== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFjYAACAAAFAAE= 1.0 eAFjYAACAAAFAAE= 1.0 eAFzyi9KSS36/48BAgE6EwdW eAFTdnN2NTN0+6/sZmloZGD2370osfL/P6Z/TABptgma 1 eAENzDEKgDAMBdAbZfAK6uCkm6ME/WAhtqX5ETy9Oj94460WylTyoFRZWtlxRINL r7aHKTFlInvi82NFY4JvnczBGnRZz2LoYeZy/EMirvcDmGwh5g== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAFzyi9KSS36/48BAgE6EwdW colorset@rainbow Border 1 colorset@rainbow Border Border 0 MaxValue:Plain Colormode Render MaxValue Images Plain Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFjYGBgYGQEAAAJAAM= 1.0 Stencils Render 10.0 eAFzyi9KSS36/48BAovzczJT/gMAb6YKcA== 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.CalculateIntensityProperties_2.Outputs.WholeCells.dataitem 3.0 0 PopulationCalculateIntensityProperties_2 1 Alexa 647 Signal channel 0 Complex s 1 1 channel_string Channel Discrete 1 CalculateIntensityProperties_2 0 1 UI.InputParameters.CalculateIntensityProperties_2.channel_string 1 eAFzzEmtSFQwMzH/DwASlwOs Alexa 647 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== Nuclei Select population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAENzDEKgDAMBdAbZfEI6uCkm6ME/WAhtqX5ETy9Oj94460WylTyoFRZWtlxRINL r7aHKTFlInvi82NFY4JvnczBGnRZz2LoYeZy/EMirvcDmIoh5w== eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf+DwDILA0U eAFjBwAACAAI 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAELTs1JTS5xTs3JCUpNz8zP+w8AO58HRA== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 CalculateIntensityProperties_2 0 1 UI.InputParameters.CalculateIntensityProperties_2.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc38DwALhgNg Cell Select region which should be characterized. 0 Complex s Region Region Discrete 1 CalculateIntensityProperties_2 0 1 eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAELSk3PzM/7DwALcgNk eAGLD0pNz8zPM/0PABGZA/g= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAELTs1JTS5xTs3JCUpNz8zP+w8AO58HRA== eAEz+w8AAW0BNg== eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAH7DwABAAEA eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjYWBgAAAAFAAF 1.0 5.0 UI.InputParameters.CalculateIntensityProperties_2.Region 1 eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== Cytoplasm Region eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== Standard Select a method 0 Complex s Method Method Discrete 0 0 0 1 0 0 50 0 0 0 CalculateIntensityProperties_2 0 1 UI.InputParameters.CalculateIntensityProperties_2.Method.title 1 eAELLknMS0ksSvkPABJkBDE= Standard eAELLknMS0ksSvkPABJkBDE= 1 Turn the Mean Intensity calculation ON/OFF 0 Complex b Mean_ONOFF Mean 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.Mean_ONOFF 1 0 1 1 0 Turn the Standard Deviation (StdDev) calculation ON/OFF 0 Complex b Stddev_ONOFF Standard Deviation 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.Stddev_ONOFF 1 0 0 1 0 Turn the Coefficient of Variance (CV) calculation ON/OFF 0 Complex b CV_ONOFF Coefficient of Variance 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.CV_ONOFF 1 0 0 1 0 Turn the Meadian Intensity calculation ON/OFF 0 Complex b Median_ONOFF Median 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.More.Median_ONOFF 1 0 0 1 0 Turn the summarized/integral intensity calculation ON/OFF 0 Complex b Sum_ONOFF Sum 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.More.Sum_ONOFF 1 0 0 1 0 Turn the Maximum Intensity calculation ON/OFF 0 Complex b Max_ONOFF Maximum 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.More.Max_ONOFF 1 0 0 1 0 Turn the Minimum Intensity calculation ON/OFF 0 Complex b Min_ONOFF Minimum 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.More.Min_ONOFF 1 0 0 1 50 For the quantile calculation all pixels are sorted according to their intensity. The quantile is the intensity of that pixel, which is brighter than the QuantileFraction number of pixels. The statistic "median" is the shorthand for "quantile" at 0.5. 0 Complex d QuantileFraction Quantile Fraction 100 0 Numeric 0 CalculateIntensityProperties_2 0 1 5 UI.InputParameters.CalculateIntensityProperties_2.Method.More.QuantileFraction 1 1 % 50 10 0 Turn the Quantile Intensity calculation ON/OFF 0 Complex b Quantile_ONOFF Numeric 0 CalculateIntensityProperties_2 0 1 UI.InputParameters.CalculateIntensityProperties_2.Method.More.QuantileFraction.qualifier 1 0 0 0 Turn the Contrast calculation ON/OFF 0 Complex b Contrast_ONOFF Contrast 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.More.Contrast_ONOFF 1 0 0 1 Intensity Cytoplasm Region Alexa 647 Prefix for property names. 0 Complex s SuffixForPropertyNames Output Properties Numeric 1 Intensity Cytoplasm Region Alexa 647 Intensity Cytoplasm Region Alexa 647 0 0 CalculateIntensityProperties_2 0 1 UI.InputParameters.CalculateIntensityProperties_2.SuffixForPropertyNames 1 0 Intensity Cytoplasm Region Alexa 647 124082 122842 3 1 3 5.0.1.124082 0 Output Property Intensity Cytoplasm Region Alexa 647 Mean eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAF9j0EKwzAMBL/iB+TULwQCgcZ9glBdEdQqdonklLzeScHt0aeF3WFgATzNaLzR baNVcCftUUIWNBoXnAkuBcBjbM5tRVNQfA5CXMbIxihuQn11rpbX9HEhRVtRzaX7 k4Lpf6zMwPHR75begroAi2Q9+e+jn7qCNQ4CImWX eAFtjLEKgDAMRPXbOglaR0eJJWg0tGBS/fvWblZwe3e8O4srKF1IzFH0LBx8tmA/ OTWptdExUu48KQEPIEfuw22CLyPRcdnRqeRpC4wGmWuc37ML/5W6fQDeM0G5 eAFVizEOgCAMANWX8BcmEsXRGUmj1aYkUvT3VBzNLXfDediC4A2OqGS5midWH/jX tau9L5EA1TEKBjJTyKeO6TE2cduymHk9IErWOix7IrBA9MXHC8gZLK4= eAG1k8FugzAQRP/Ih+YTCJGQWhIlhx7RFkZ0m8VGeE3F17duIsUXDiShRx/meTwz zkeSQMrObknJHAZXowkDvMlI6iCkKDpqUb2YfdA+qDcl2igYwSLB63ARmyaqWdH9 5A8AqXyOtbK1hLvf2I5tU4ZawIlyPS5IaEZcWFYmeSN/XpLxDOHVfWfOxp687j++ UMcGF1rJJnW9kO9uT3n/dIIMIqswqtT6iCWmTpDo/+/+I9o42Qd9pWVbhfWsU5x9 j0EZvtr8BzR9nrkEfwHXAGZH eAFjYIADRgAADQAC 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5hAECTgXFmS X5CTWJwLFQMAh2Igiw== 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MethodFolder InputParameters.CalculateIntensityProperties_2.Method Method Region Method 0 0 0 MoreFolder InputParameters.CalculateIntensityProperties_2.Method.More CV_ONOFF 0 0 0 1 builtin Each SelectPopulation_4 1 Replace me 1 1 Nuclei PopulationSelectPopulation_4 Population: Nuclei 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAELTs1JTS5JTVHwT8oCMor/u2QWJycWpSCJAAD5nw5/ Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationSelectPopulation_4 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 MaxValue:Plain eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 MaxValue eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Plain eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjYAQCAAAPAAU= 1.0 eAFzL0qs/P+PAQIBLrQGiw== eAFzL0qs/P+PAQIBLrQGiw== eAFjZAACAAAKAAI= 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjZAACAAAKAAI= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFLKLnoKn43x4EBBE5tg9DHD4Fph4A7cD4A5fIKiQ== 1.0 eAF7/vdYoYx6pMNkmxT3/6siHQKku//9LolysFRYbqEQ3+zAAAUAcEsOKw== 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7/x8MABvrBvo= eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFjYAACRkYAAAoAAw== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9z84NSc1 uSQ1RcE/KQvIKP7vklmcnFiUgiQCAEB4JPw= eAFjYAABRgAACAAC 1.0 eAFjYAADAAAHAAE= 1.0 eAFzyk+p/P+PAQKd8otSUov+/2MEAH0hCuI= eAFzL0qs/K/sZmloZGD2/x8DBAIAXqwI9Q== 1 eAEdzDEKgDAMQNEbZfIIKjhZcHAsoQ1YiKY0idfX0vl9/vIiO1qRZ0ZDCE0SZW+k cBBTsiDVeXicYHerbgrnJd2IWeMqbWN2tTYiyP1SjO7vBxyuI6E= eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFjYAACRkYAAAoAAw== 1.0 eAFzyk+p/P+PAQKTwBxGAGo0CjI= green body 1 colorset@rainbow Body colorset@rainbow Body Body 0 MaxValue:Plain Colormode Render MaxValue Images Plain Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8V3ZzszQ2cPwPAK5VE2o= eAFjYAACRkZGAAAOAAQ= 1.0 Stencils Render 10.0 eAFzyk+p/P+PAQKd8otSUov+/2Mszs/JTPkPAMf8Dfw= 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation_4.Outputs.Wholecells_ForIllustration.dataitem 3.0 1 AnnexinV_neg cells FilteredSelectPopulation_4 Population: AnnexinV_neg cells 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzTs3J+Q8ABgMCgA== Standard 0 eAELKMovSC0qyUwtVnDMy0utyMwLi89LTVdITs3JKf4PALZnDEA= FeedBack.FilteredSelectPopulation_4 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PAQIBLrQGiw== eAFzL0qs/P+PAQIBLrQGiw== eAFjZAACAAAKAAI= 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjYAACAAAFAAE= 1.0 eAEBKADX/5JHRsTSuuE/t/U22aWSyT/Qmb/1AqPJP8qt45GNiM8/jHO8X0xkyT/s khc0 1.0 eAELePhimuX3BAfe/EdMIZu3OZx6ycQh9uK4w9/e0j+8t086MIDAz+MOAJZ3Eos= 1.0 eAHzSenmu34rwmGyTYr7/1WRDgHS3f9+l0Q5WCost1CIb3ZggAIAXFYNxg== 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7/x8IAA72BPw= eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFjYAACAAAFAAE= 1.0 eAFjYAACAAAFAAE= 1.0 eAFzyk+p/J8EIv4xgCAARPsINQ== eAFzL0qs/P+PAQYBPcYHiQ== 1 eAEdyzEKgDAMBdAbZfIG6mzBwbGE9ouFaEqbeH0V58ebbxZnK3pNbEyhaUL2hk4r BMmCVpff40CLW3XrtB0qGCHS417E0JApf70YzucFLOEgnw== 1 PopulationSelectPopulation_4 eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAFzyk+p/J8EIv4xgCAARPsINQ== colorset@rainbow body 1 colorset@rainbow Body Body 0 1 1 Filtered Filtered Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation_4.Outputs.WholeCells_filtered.dataitem 3.0 0 PopulationSelectPopulation_4 1 Nuclei Select a population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAENzDEKgDAMBdAbZfAK6uCkm6ME/WAhtqX5ETy9Oj94460WylTyoFRZWtlxRINL r7aHKTFlInvi82NFY4JvnczBGnRZz2LoYeZy/EMirvcDmGwh5g== eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf6DwDIKg0T eAHjAAAACQAJ 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf+DwDILA0U eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 SelectPopulation_4 0 1 UI.InputParameters.SelectPopulation_4.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc38DwALhgNg Filter by Property Select a method 0 Complex s Method Method Discrete 0 SelectPopulation_4 0 1 UI.InputParameters.SelectPopulation_4.Method.title 1 eAFzy8wpSS1SSKpUCCjKL0gtKqn8DwBG/Afm Filter by Property eAFzy8wpSS1SSKpUCCjKL0gtKqn875yfm5ufp+AGlin+75OZl5pYpOCck1hcnJmW mVr0Pzg1JzW5BKTHN7E4+z8Anq0bzA== 0.0 Define a proper value for the selection criterion 0 Complex d Attribute1 3.00000000000000018e+34 -3.00000000000000018e+34 Numeric 0 SelectPopulation_4 0 0 UI.InputParameters.SelectPopulation_4.Method.Attribute1 1 0 0 eAE7ewYE1A8wQMFZCN8BQps7zJoJBDcdoXx3B7CyA75Q8UAHYxC4HAKVD3dIA4Fj UVB1sRDxmAQHAGPzMPg= 1.0 11.9000000000000004 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName1, RegionTable=ScalarTable| ScalarTable_1=TableForSelectedRegion, SelectedScalarRowIndex_1=SelectedRegionRowIndex) Complex s AttributeName1 Discrete _Scalar2>0 and _Scalar2<120 3 3 eAGLdzeM/x/vbgQijOP/AwAxDQaj 0 SelectPopulation_4 0 0 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= eAELTk7MSSz6DwALOQNW eAGLD05OzEksMvoPABFMA+c= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf6DwDIKg0T eAGz+A8AAXEBOA== eAH7DwABAAEA eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cMxJrUhUMDMx/w8AT34UnQ== eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAFzzEmtSFQwMzH/DwASlwOs eAEz+A8AAWEBMA== eAFzzi/NKynWD8isSM35DwAjwwWt eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cMxJrUhUMDMx/w8AT34UnQ== eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjZGBgAAAACAAC 1.0 5.0 UI.InputParameters.SelectPopulation_4.Method.Attribute1.label 1 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= Intensity Cytoplasm Region Alexa 647 Mean eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP0/BMSe1IlHBzMQcohIAsdIdRg== > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier1 Discrete 0 SelectPopulation_4 0 0 UI.InputParameters.SelectPopulation_4.Method.Attribute1.qualifier 1 eAGz+w8AAX0BPg== > eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB 0.0 Define a proper value for the selection criterion 0 Complex d Attribute2 3.00000000000000018e+34 -3.00000000000000018e+34 Numeric 0 SelectPopulation_4 0 0 UI.InputParameters.SelectPopulation_4.Method.Attribute2 1 0 120 eAHrP/RVI2Z/hMOsmUBgGelgDAKbIx3OngECnSiHNBBYFuXAAAIK0RB6QTREvWQM RP3kGIh6nliI+rZYiDqGOIi6yjgHAFWCJo8= 1.0 1.90999999999999992 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName2, RegionTable=ScalarTable| ScalarTable_2=TableForSelectedRegion, SelectedScalarRowIndex_2=SelectedRegionRowIndex) Complex s AttributeName2 Discrete 0 SelectPopulation_4 0 0 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= eAELTk7MSSz6DwALOQNW eAGLD05OzEksMvoPABFMA+c= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf6DwDIKg0T eAGz+A8AAXEBOA== eAH7DwABAAEA eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cMxJrUhUMDMx/w8AT34UnQ== eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAFzzEmtSFQwMzH/DwASlwOs eAEz+A8AAWEBMA== eAFzzi/NKynWD8isSM35DwAjwwWt eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cMxJrUhUMDMx/w8AT34UnQ== eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjZGBgAAAACAAC 1.0 5.0 UI.InputParameters.SelectPopulation_4.Method.Attribute2.label 1 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= Intensity Cytoplasm Region Alexa 647 Mean eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP0/BMSe1IlHBzMQcohIAsdIdRg== > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier2 Discrete 0 SelectPopulation_4 0 0 UI.InputParameters.SelectPopulation_4.Method.Attribute2.qualifier 1 eAGz+Q8AAXkBPA== < eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB 0.0 Define a proper value for the selection criterion 0 Complex d Attribute3 Numeric 0 SelectPopulation_4 0 0 UI.InputParameters.SelectPopulation_4.Method.Attribute3 1 0 0 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName3, RegionTable=ScalarTable| ScalarTable_3=TableForSelectedRegion, SelectedScalarRowIndex_3=SelectedRegionRowIndex) Complex s AttributeName3 Discrete 0 SelectPopulation_4 0 0 eAHzy89L/Q8ABloCkA== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA UI.InputParameters.SelectPopulation_4.Method.Attribute3.label 1 eAH7DwABAAEA eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP0/BMSe1IlHBzMQcohIAsdIdRg== > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier3 Discrete 0 SelectPopulation_4 0 0 UI.InputParameters.SelectPopulation_4.Method.Attribute3.qualifier 1 eAGz+w8AAX0BPg== > eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB F1 and F2 Defines boolean operations between filters, e.g. (F1 or F2) and F3. As default AND operations are applied. F1, F2 ... correspond to the first and to the second filter, respectively. 0 Complex s BooleanOperations Boolean Operations Numeric 1 F1 and F2 0 SelectPopulation_4 0 1 UI.InputParameters.SelectPopulation_4.Method.More.BooleanOperations 1 0 F1 and F2 Nuclei Selected Name of the output population. 0 Complex s OutputName Output Population Numeric 0 Nuclei Selected Nuclei Selected 1 0 SelectPopulation_4 0 1 UI.InputParameters.SelectPopulation_4.OutputName 1 0 AnnexinV_neg cells 124082 122842 4.0 1 eAFzzMtLrcjMC4vPS01XSE7NySn+DwBJzgfz 4.0 5.0.1.124082 0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAELSk3PzM/7/48BAgE6agdc eAHzK03OSS0t/h8flJqemZ9nBGMYwxgmMIbpfwC2LBO1 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFzy8xL8StNzknN/O8GZDpXluQX5CQW5/7/x/iPMTg1JzW5xDk1JycoNT0zP+8/ ANRdFF4= eAEz+W/6/x/jP0az/wAfCQWb eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAH775yak6OQVKmQnJGYl5eao+CcmJNcmpNYkpqi4JmbmJ6qo5CbWpKRn6Lg9B+i NL8oJbWIWB2VJfkFOYnFuUSq/w8AB+c1/g== eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmfZ636HwBf fxbe eAH7/x8IAA72BPw= eAHzyE9NziguUfBNLM7+75yYk1yak1iSmqLgmZuYnvr/H+M/xv8AAJwPaw== eAEz+P+PAQIBHrkFKA== eAH7/x8IAA72BPw= eAH7/x8IAA72BPw= eAH7/x8IAA72BPw= eAFL+f+PAQIBIMEFXA== eAH7/x8IAA72BPw= eAH7/x8IAA72BPw= 5.0 Output Population AnnexinV_neg cells eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAF9kNEKglAMhl/lPEBX0QuEIAhlQVCXY52GnZqbeKbl05uGCd14Nbbv52MbQE4F Wmjp0FLN2FFMkH3DaJSVWBCse4AcZREvKxYFfd54ptBnEiwguz3G58pNw52+nFex GqM5vT7IW5zhlEmD3JLOtGKMJQTmJg758aKfegr+l60IvYOcQahwnpjjsOblrkzf BlKts9mlcqIB2FGr8TNBBTb9B2s3hGU= eAGFTssKgzAQbL9NEAQbjz2GmI7t6pKFZLV/n2jxUAtCbzPDvAyeTmkBMc9J44Yl FOPMD8+XfDWzZ1BpAik5vrk0lVbelYQtlLTrR3hN5f4SRgXmI7TfsgXnlr+qHYgV EY/d6T8TtpbYHH+vmsFa6g== eAFVjVEKgCAQRKuTeBchCMo++wyTpazFhdzq9loGETI/b2YYRsGs2Z7QIB6e94fJ RaVd5kMRSnUYBBsbZ9lqFJ32W2zpEpLcM/Ms+mkFwz6GalgIQQJiMp/+cHzRpH6s ac+ebpXwPqA= eAG1lMFOwzAQRP/Ih8IfhFaKVNIKDhytrbOEhY0d2eugfn3rUKm+BMkN5ejDPM/u jL0egSMIOfsEAmrvncE2egyqAjaRQbDuoUO9UrsoQ5SgGuySYERijkH8j1i1SU2C /Wm9AAjN31h3tpZxtxvbkG2baBgpUy7Hgg3NiGtLQsDPEL5KdjxD2LrvytmUU5Dd 4RNNSrDQSnUUNzCE/jrK24djrJD5LgydUx+xxNQrcvI/3f+CXarsQl+52VbQBpJj qv2AXgiDfvgPaH48t23wMvDeDdNDTAPrx4XuikD6nVjQY1uexe/WzNQSvXG+nv0m zmd9x3U= eAFjYEAARkYGAAAUAAM= 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5hAECTgXFmS X5CTWJwLFfvHFJyak5pcEpBfADIvMz8v3uT/PxYA6asqqg== eAGNjkEKwjAQRbXeqPUGAcGNlHqAENJPGZgmoZkuevkZLS6kG3H534fHc4HjykFw n8ME35qe3AGZnm+UxscaGWTaaLNPt0kuHOpsenmCEcWBecBEOdlXkASpkmz9kgsW IVTf/b5b++j6XPast85f/0Qvw8BXSw== eAFjYmJmZmFhYWVlY+fg5OQEAAH0AFE= 1.0 eAFjYEABAAAPAAE= 1.0 eAFjYmZiZmRmYWRiBAImZgABEQAf 1.0 eAFjYGBgYGVgYGNg5+AEMgEA7AAk 1.0 eAFjZAQDBkYGIGBkBAAAbQAL 1.0 eAF9jkEKgzAQRWt7I9sbBIRuitgDhJB+ZGBMghkXXj5jgwsRSvm79+HxjGO/sBM8 JzfCtlou5oS0NB2Fz2vxDNJyravArBITuzxpub3B8GLAPGCkGPRQBEHIJGs/x4RZ CNne/9+t7ro+phr21dnHL7QBSkBQLg== eAH7////f7/8vFQg9Y8BiGDwPwDpchB8 eAH7/x8FAAB3lw7y eAFjYEABAAAPAAE= 1.0 eAH7////f4/81OSM4hIF38TibCD3HwMQQeD//wBBvxNn eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA eAELKUrMzMvMS3dJLEn8DwAkdQW2 1 Method MethodFolder InputParameters.SelectPopulation_4.Method Method WholeCells_string Method 1 1 0 MoreFolder InputParameters.SelectPopulation_4.Method.More AttributeQualifier3 0 0 0 1 builtin Each SelectPopulation_5 1 Replace me 1 1 Nuclei PopulationSelectPopulation_5 Population: Nuclei 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAELTs1JTS5JTVHwT8oCMor/u2QWJycWpSCJAAD5nw5/ Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationSelectPopulation_5 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 MaxValue:Plain eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 MaxValue eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Plain eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjYAQCAAAPAAU= 1.0 eAFTdnN2dTM1+O9elFj53ymnNPX/P8Z/jABhKgmz eAFzL0qs/P+PAQIBLrQGiw== eAFjZGBkYAAAAA0AAw== 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjZGBkZAAAAA8ABA== 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAEz2PViUfe7egcGEDi1DUIfPwSmHQLuwPkADy8Lqw== 1.0 eAGz1LZXPGcU6TDZJsX9/6pIhwDp7n+/S6IcLBWWWyjENzswQAEAMAwMkQ== 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7/x8MABvrBvo= eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFjYAACRkYAAAoAAw== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9z84NSc1 uSQ1RcE/KQvIKP7vklmcnFiUgiQCAEB4JPw= eAFjYAACRkYAAAoAAw== 1.0 eAFjYAADAAAHAAE= 1.0 eAFzyk+p/P+PAQKd8otSUov+/2MEAH0hCuI= eAFTdnN2dTM1+O9elFj5/x+jU05pKpD6xwgAdnoKsg== 1 eAEdzDEKgDAMQNEbZfMGKjhZcHAsoQ1YiKY0idfX0vl9/vIiO1qRZ0ZDCE0SZW+k cBBTsiDVeXicYHerbgrnJd2IWeMqbWN2tTYiyP1SjO7vBxzcI6I= eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFjYAACRkYAAAoAAw== 1.0 eAFzyk+p/P+PAQKTwBxGAGo0CjI= green body 1 colorset@rainbow Body colorset@rainbow Body Body 0 MaxValue:Plain Colormode Render MaxValue Images Plain Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8V3ZzszQ2cPwPAK5VE2o= eAFjYAACRkZGAAAOAAQ= 1.0 Stencils Render 10.0 eAFzyk+p/P+PAQKd8otSUov+/2Mszs/JTPkPAMf8Dfw= 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation_5.Outputs.Wholecells_ForIllustration.dataitem 3.0 1 AnnexinV_pos cell FilteredSelectPopulation_5 Population: AnnexinV_pos cell 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzTs3J+Q8ABgMCgA== Standard 0 eAELKMovSC0qyUwtVnDMy0utyMwLiy/IL1ZITs3J+Q8Aq2kL5Q== FeedBack.FilteredSelectPopulation_5 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PAQIBLrQGiw== eAFzL0qs/P+PAQIBLrQGiw== eAFjZAACAAAKAAI= 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjYAACAAAFAAE= 1.0 eAEBKADX/5JHRsTSuuE/t/U22aWSyT/Qmb/1AqPJP8qt45GNiM8/jHO8X0xkyT/s khc0 1.0 eAELePhimuX3BAfe/EdMIZu3OZx6ycQh9uK4w9/e0j+8t086MIDAz+MOAJZ3Eos= 1.0 eAHzSenmu34rwmGyTYr7/1WRDgHS3f9+l0Q5WCost1CIb3ZggAIAXFYNxg== 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7/x8IAA72BPw= eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFjYAACAAAFAAE= 1.0 eAFjYAACAAAFAAE= 1.0 eAFzyk+p/J8EIv4xgCAARPsINQ== eAFzL0qs/P+PAQYBPcYHiQ== 1 eAEdyzEKgDAMBdAbZfME6mzBwbGE9ouFaEqbeH0V58ebbxZnK3pNbEyhaUL2hk4r BMmCVpff40CLW3XrtB0qGCHS417E0JApf70YzucFLQggoA== 1 PopulationSelectPopulation_5 eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAFzyk+p/J8EIv4xgCAARPsINQ== colorset@rainbow body 1 colorset@rainbow Body Body 0 1 1 Filtered Filtered Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation_5.Outputs.WholeCells_filtered.dataitem 3.0 0 PopulationSelectPopulation_5 1 Nuclei Select a population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAEdyzEKgDAMBdAbZfIG6mzBwVFC+8FCNKVJvL6K8+PNN0uwV70mdqbUNaNEh9EK QfakLeT3faAlvIUbbYcKRogYlW9Vx/m8GWkc8Q== eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAELTs1JTS4JyC8ozUksyczPizf5DwBMegge eAHjBAAACgAK 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf6DwDIKg0T eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 SelectPopulation_5 0 1 UI.InputParameters.SelectPopulation_5.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc3875iXl1qRmRcWn5earpCcmpNT/B8AlWELUg== Filter by Property Select a method 0 Complex s Method Method Discrete 0 SelectPopulation_5 0 1 UI.InputParameters.SelectPopulation_5.Method.title 1 eAFzy8wpSS1SSKpUCCjKL0gtKqn8DwBG/Afm Filter by Property eAFzy8wpSS1SSKpUCCjKL0gtKqn875yfm5ufp+AGlin+75OZl5pYpOCck1hcnJmW mVr0Pzg1JzW5BKTHN7E4+z8Anq0bzA== 0.0 Define a proper value for the selection criterion 0 Complex d Attribute1 Numeric 0 SelectPopulation_5 0 0 UI.InputParameters.SelectPopulation_5.Method.Attribute1 1 0 0 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName1, RegionTable=ScalarTable| ScalarTable_1=TableForSelectedRegion, SelectedScalarRowIndex_1=SelectedRegionRowIndex) Complex s AttributeName1 Discrete _Scalar2>=120 3 3 eAGLdzeM/x/vbgQijOP/AwAxDQaj 0 SelectPopulation_5 0 0 eAHzy89L/Q8ABloCkA== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA UI.InputParameters.SelectPopulation_5.Method.Attribute1.label 1 eAH7DwABAAEA eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP0/BMSe1IlHBzMQcotIxLy+1IjMvLD4vNV0hOTUnp/g/ACfsJTg= > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier1 Discrete 0 SelectPopulation_5 0 0 UI.InputParameters.SelectPopulation_5.Method.Attribute1.qualifier 1 eAGz+w8AAX0BPg== > eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB 0.0 Define a proper value for the selection criterion 0 Complex d Attribute2 3.00000000000000018e+34 -3.00000000000000018e+34 Numeric 0 SelectPopulation_5 0 0 UI.InputParameters.SelectPopulation_5.Method.Attribute2 1 0 120 eAHrP/RVI2Z/hMOsmUBgGelgDAKbIx3OngECnSiHNBBYFuXAAAIK0RB6QTREvWQM RP3kGIh6nliI+rZYiDqGOIi6yjgHAFWCJo8= 1.0 1.90999999999999992 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName2, RegionTable=ScalarTable| ScalarTable_2=TableForSelectedRegion, SelectedScalarRowIndex_2=SelectedRegionRowIndex) Complex s AttributeName2 Discrete 0 SelectPopulation_5 0 0 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= eAELTk7MSSz6DwALOQNW eAGLD05OzEksMvoPABFMA+c= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf6DwDIKg0T eAGz+A8AAXEBOA== eAH7DwABAAEA eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cMxJrUhUMDMx/w8AT34UnQ== eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAFzzEmtSFQwMzH/DwASlwOs eAEz+A8AAWEBMA== eAFzzi/NKynWD8isSM35DwAjwwWt eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cMxJrUhUMDMx/w8AT34UnQ== eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjZGBgAAAACAAC 1.0 5.0 UI.InputParameters.SelectPopulation_5.Method.Attribute2.label 1 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= Intensity Cytoplasm Region Alexa 647 Mean eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP0/BMSe1IlHBzMQcotIxLy+1IjMvLD4vNV0hOTUnp/g/ACfsJTg= > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier2 Discrete 0 SelectPopulation_5 0 0 UI.InputParameters.SelectPopulation_5.Method.Attribute2.qualifier 1 eAGzs/0PAAI2AXs= >= eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB 0.0 Define a proper value for the selection criterion 0 Complex d Attribute3 Numeric 0 SelectPopulation_5 0 0 UI.InputParameters.SelectPopulation_5.Method.Attribute3 1 0 0 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName3, RegionTable=ScalarTable| ScalarTable_3=TableForSelectedRegion, SelectedScalarRowIndex_3=SelectedRegionRowIndex) Complex s AttributeName3 Discrete 0 SelectPopulation_5 0 0 eAHzy89L/Q8ABloCkA== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA UI.InputParameters.SelectPopulation_5.Method.Attribute3.label 1 eAH7DwABAAEA eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP0/BMSe1IlHBzMQcotIxLy+1IjMvLD4vNV0hOTUnp/g/ACfsJTg= > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier3 Discrete 0 SelectPopulation_5 0 0 UI.InputParameters.SelectPopulation_5.Method.Attribute3.qualifier 1 eAGz+w8AAX0BPg== > eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB Nuclei Selected Name of the output population. 0 Complex s OutputName Output Population Numeric 0 Nuclei Selected Nuclei Selected 1 0 SelectPopulation_5 0 1 UI.InputParameters.SelectPopulation_5.OutputName 1 0 AnnexinV_pos cell 124082 122842 4.0 1 eAFzzMtLrcjMC4svyC9WSE7NyfkPAEMdB5g= 4.0 5.0.1.124082 0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAELSk3PzM/7/48BAgE6agdc eAHzK03OSS0t/h8flJqemZ9nBGMYwxgmMIbpfwC2LBO1 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFzy8xL8StNzknN/O8GZDpXluQX5CQW5/7/x/iPMTg1JzW5xDk1JycoNT0zP+8/ ANRdFF4= eAEz+W/6/x/jP0az/wAfCQWb eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAH775yak6OQVKmQnJGYl5eao+CcmJNcmpNYkpqi4JmbmJ6qo5CbWpKRn6Lg9B+i NL8oJbWIWB2VJfkFOYnFuUSq/w8AB+c1/g== eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmfZ636HwBf fxbe eAH7/x8IAA72BPw= eAHzyE9NziguUfBNLM7+75yYk1yak1iSmqLgmZuYnvr/H+M/xv8AAJwPaw== eAEz+P+PAQIBHrkFKA== eAH7/x8IAA72BPw= eAH7/x8IAA72BPw= eAH7/x8IAA72BPw= eAFL+f+PAQIBIMEFXA== eAH7/x8IAA72BPw= eAH7/x8IAA72BPw= 5.0 Output Population AnnexinV_pos cell eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= 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eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA eAELKUrMzMvMS3dJLEn8DwAkdQW2 1 Method MethodFolder InputParameters.SelectPopulation_5.Method Method WholeCells_string Method 1 1 0 1 builtin Each CalculateIntensityProperties_4 1 Replace me 1 1 Nuclei PopulationCalculateIntensityProperties_4 Population: Nuclei 0 sticky eAFzdXcL+A8ABOsCIg== eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationCalculateIntensityProperties_4 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PAQIBLrQGiw== eAFzL0qs/P+PAQIBLrQGiw== eAFjYGRgYAAAAAkAAg== 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjYAACAAAFAAE= 1.0 eAEBKADX/3S6+ni9j+E/AZ2mGfMr4T8yAQrtVrXUP938SUyEHtY/Y0KihvFe1D+n rxRE 1.0 eAGbPg0IviU4HJu/WijndpLDHnuWPfapGx0Uv61863twswMDCKzY6AAAnjESKA== 1.0 eAFbuQIIXkQ4FB9YcUv9ZKTDf4EP/wQMoh3++G2YNGFpswMDFAAAuE8RZA== 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7/x8IAA72BPw= eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGBgYAQAAAYAAg== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFjYAACAAAFAAE= 1.0 eAFjYAACAAAFAAE= 1.0 eAFzyi9KSS36/48BAgE6EwdW eAFzL0qs/P+PAQYBPcYHiQ== 1 eAENzDEKgDAMBdAbZfIG6uCkm6ME/WAhtqX5ETy9Oj94460WylTyoFRZWtlxRINL r7aHKTFlInvi82NFY4JvnczBGnRZz2LoYeZy/EMirvcDmKgh6A== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAFzyi9KSS36/48BAgE6EwdW colorset@rainbow Border 1 colorset@rainbow Border Border 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFjYGBgYGQEAAAJAAM= 1.0 Stencils Render 10.0 eAFzyi9KSS36/48BAovzczJT/gMAb6YKcA== 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.CalculateIntensityProperties_4.Outputs.WholeCells.dataitem 3.0 0 PopulationCalculateIntensityProperties_4 1 Alexa 647 Signal channel 0 Complex s 1 1 channel_string Channel Discrete 1 CalculateIntensityProperties_4 0 1 UI.InputParameters.CalculateIntensityProperties_4.channel_string 1 eAFzdXcL+A8ABOsCIg== EGFP eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== Nuclei Select population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAEdyzEKgDAMBdAbZfME6mzBwVFC+8FCNKVJvL6K8+PNN0uwV70mdqbUNaNEh9EK QfakLeT3faAlvIUbbYcKRogYlW9Vx/m8GYcc8g== eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAELTs1JTS4JyC8ozUksyczPizf9DwBMfAgf eAHjAgAACwAL 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAELTs1JTS4JyC8ozUksyczPizf5DwBMegge eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 CalculateIntensityProperties_4 0 1 UI.InputParameters.CalculateIntensityProperties_4.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc3875iXl1qRmRcWn5earpCcmpNTjBAqyC8GC/0HAKQ/Euk= Cell Select region which should be characterized. 0 Complex s Region Region Discrete 1 CalculateIntensityProperties_4 0 1 eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAELSk3PzM/7DwALcgNk eAGLD0pNz8zPM/0PABGZA/g= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAELTs1JTS5xTs3JCUpNz8zP+w8AO58HRA== eAEz+w8AAW0BNg== eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAH7DwABAAEA eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjYWBgAAAAFAAF 1.0 5.0 UI.InputParameters.CalculateIntensityProperties_4.Region 1 eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== Cytoplasm Region eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== Standard Select a method 0 Complex s Method Method Discrete 0 0 0 1 0 0 50 0 0 0 CalculateIntensityProperties_4 0 1 UI.InputParameters.CalculateIntensityProperties_4.Method.title 1 eAELLknMS0ksSvkPABJkBDE= Standard eAELLknMS0ksSvkPABJkBDE= 1 Turn the Mean Intensity calculation ON/OFF 0 Complex b Mean_ONOFF Mean 1 0 Numeric 0 CalculateIntensityProperties_4 0 1 1 UI.InputParameters.CalculateIntensityProperties_4.Method.Mean_ONOFF 1 0 1 1 0 Turn the Standard Deviation (StdDev) calculation ON/OFF 0 Complex b Stddev_ONOFF Standard Deviation 1 0 Numeric 0 CalculateIntensityProperties_4 0 1 1 UI.InputParameters.CalculateIntensityProperties_4.Method.Stddev_ONOFF 1 0 0 1 0 Turn the Coefficient of Variance (CV) calculation ON/OFF 0 Complex b CV_ONOFF Coefficient of Variance 1 0 Numeric 0 CalculateIntensityProperties_4 0 1 1 UI.InputParameters.CalculateIntensityProperties_4.Method.CV_ONOFF 1 0 0 1 0 Turn the Meadian Intensity calculation ON/OFF 0 Complex b Median_ONOFF Median 1 0 Numeric 0 CalculateIntensityProperties_4 0 1 1 UI.InputParameters.CalculateIntensityProperties_4.Method.More.Median_ONOFF 1 0 0 1 0 Turn the summarized/integral intensity calculation ON/OFF 0 Complex b Sum_ONOFF Sum 1 0 Numeric 0 CalculateIntensityProperties_4 0 1 1 UI.InputParameters.CalculateIntensityProperties_4.Method.More.Sum_ONOFF 1 0 0 1 0 Turn the Maximum Intensity calculation ON/OFF 0 Complex b Max_ONOFF Maximum 1 0 Numeric 0 CalculateIntensityProperties_4 0 1 1 UI.InputParameters.CalculateIntensityProperties_4.Method.More.Max_ONOFF 1 0 0 1 0 Turn the Minimum Intensity calculation ON/OFF 0 Complex b Min_ONOFF Minimum 1 0 Numeric 0 CalculateIntensityProperties_4 0 1 1 UI.InputParameters.CalculateIntensityProperties_4.Method.More.Min_ONOFF 1 0 0 1 50 For the quantile calculation all pixels are sorted according to their intensity. The quantile is the intensity of that pixel, which is brighter than the QuantileFraction number of pixels. The statistic "median" is the shorthand for "quantile" at 0.5. 0 Complex d QuantileFraction Quantile Fraction 100 0 Numeric 0 CalculateIntensityProperties_4 0 1 5 UI.InputParameters.CalculateIntensityProperties_4.Method.More.QuantileFraction 1 1 % 50 10 0 Turn the Quantile Intensity calculation ON/OFF 0 Complex b Quantile_ONOFF Numeric 0 CalculateIntensityProperties_4 0 1 UI.InputParameters.CalculateIntensityProperties_4.Method.More.QuantileFraction.qualifier 1 0 0 0 Turn the Contrast calculation ON/OFF 0 Complex b Contrast_ONOFF Contrast 1 0 Numeric 0 CalculateIntensityProperties_4 0 1 1 UI.InputParameters.CalculateIntensityProperties_4.Method.More.Contrast_ONOFF 1 0 0 1 Intensity Cytoplasm Region EGFP Prefix for property names. 0 Complex s SuffixForPropertyNames Output Properties Numeric 1 Intensity Cytoplasm Region EGFP Intensity Cytoplasm Region EGFP 0 0 CalculateIntensityProperties_4 0 1 UI.InputParameters.CalculateIntensityProperties_4.SuffixForPropertyNames 1 0 Intensity Cytoplasm Region EGFP 124082 124082 3 1 3 5.0.1.124082 0 Output Property Intensity Cytoplasm Region EGFP Mean eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAGV0FEKgkAQBuCr7AF6ijpABIJQFgT1OEzbYFvjjLir5elNRYUghJ6Wnf/nY2cB EkoxuIoOFRWMNfktsi0ZA8UZpgTLBiBBmY3niVmgSUrL5JpYXHDIZo/+uTDDcKcv Y1VCgT4YvT7IBj+FQydyctvWQXNGn4FjLn3b7zYa6aH4fWxE6O3kDEKpscTs22de 7srUXyDSIp4slRO1QThq3v2MU4HVqP+AplGuvrf/o9cj/QEYLq4q eAGVjsEKwkAMRPWPSr+gUigUdL3pMWzXWKMhC7tp9eu7Vj24giDeJmFm3hjsrdKI xDxEDbP2kow1H/e0mJZmcIyUWiElyxsbL2ntr7WXORR1253RaUz7k2eskTmX8C4b 8bvl5xeOxIoBDy+neyCg8aHNd2d0gGolgjeSHQj2VVk8I0UG+rfyDid8gq4= eAGVTcsKgCAQrL7EfwmCoOzYMUyWshYXcqu/1x4U4THmMC+GkTAoNhuUiKvj5dRk g1Q28j7xqVw1ggmlNWwUilq5OVS0i5zsOXMsmn4CzS74rB0JIQfEy7z4wu6W+uq7 gpbo6XmJ6df0AI6iXA4= eAG1lMFOwzAQRP/Iqgrci0IrRYK0AgmO1pIswbCxI3sd6NeD00pYglI5aXr0YZ9n dsZedkAeWBl9AwxiY02JlbfoRAZUegLGvIEa5VysPbeenSiwDgMdKiLv2O6GRRWm FWPztRwBhOI01sTSIm64sJXSVeFLQhUp+2PChg4M51qxAroD956y4wOEW/ORGR1y 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eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA 1 Method MethodFolder InputParameters.CalculateIntensityProperties_4.Method Method Region Method 0 0 0 MoreFolder InputParameters.CalculateIntensityProperties_4.Method.More CV_ONOFF 0 0 0 1 builtin Each SelectPopulation 1 Replace me 1 1 Nuclei PopulationSelectPopulation Population: Nuclei 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAELTs1JTS5JTVHwT8oCMor/u2QWJycWpSCJAAD5nw5/ Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationSelectPopulation eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 eAFjAAAAAQAB 1.0 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 eAFjAAAAAQAB 1.0 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 eAFjAAAAAQAB 1.0 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 eAFjAAAAAQAB 1.0 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PAQIBLrQGiw== eAFzL0qs/P+PAQIBLrQGiw== eAFjYGRgYAAAAAkAAg== 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjYAACAAAFAAE= 1.0 eAEBKADX/+pL5Y4bweE/+9gXIGz34D9iL/gfSFbTP0hawyRwX9Q/aWLznAgb0z+s 8xOD 1.0 eAGbGDGpmvVtgsNn5/+Cx42SHfLz8vLeOe53EPv6gzvowgEHBhCo2u8AAHrkEPs= 1.0 eAGTsK/J1Xod4bD+59mag3mRDikg0BLlsGJdgcP9qGYHBigAAGblDrs= 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7/x8CACPkB/k= eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8V3ZzszQ2cPwPAK5VE2o= eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8V3ZzszQ2cPwPAK5VE2o= eAFjYAACRgZGAAAMAAM= 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9z84NSc1 uSQ1RcE/KQvIKP7vklmcnFiUgiTikZmekQPEyKoAdO4tRg== eAFjYIAAAAAIAAE= 1.0 eAFjYAADRgAACQAC 1.0 eAFzyk+p/P+PAQKTwBzG4vyczJT/ALDvDUw= eAFzL0qs/P+PAQIBLrQGiw== 1 eAElzEsKgDAMANEb5RQquLLgwqWENmAhmpKP19ei6zfMeCMHepVrQEdIKplKKBms xJQ9SQv+HJbwFm6wHdKFmG2fRGfmMNc/Kf1Rnc7nBeCqIw4= eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFjYAACRkYAAAoAAw== 1.0 eAFzyk+p/P+PAQKTwBxGAGo0CjI= green body 1 colorset@rainbow Body colorset@rainbow Body Body 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8V3ZzszQ2cPwPAK5VE2o= eAFjYAACRgZGAAAMAAM= 1.0 Stencils Render 10.0 eAFzyk+p/P+PAQKTwBzG4vyczJT/ALDvDUw= 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation.Outputs.Wholecells_ForIllustration.dataitem 0 UI_in=UI; UI=ocnt(); UI::C_Layout("(1)", vec("Controls"), cellalign="center", class="packer") Buttons=UI; UI=UI_in; 3.0 1 Transfected cell FilteredSelectPopulation Population: Transfected cell 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzTs3J+Q8ABgMCgA== Standard 0 eAELKMovSC0qyUwtVggpSswrTktNLklNUUhOzcn5DwCgoQuA FeedBack.FilteredSelectPopulation eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 eAFjAAAAAQAB 1.0 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 eAFjAAAAAQAB 1.0 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 eAFjAAAAAQAB 1.0 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 eAFjAAAAAQAB 1.0 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PAQIBLrQGiw== eAFzL0qs/P+PAQIBLrQGiw== eAFjZAACAAAKAAI= 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjYAACAAAFAAE= 1.0 eAEBKADX/+pL5Y4bweE/+9gXIGz34D9iL/gfSFbTP0hawyRwX9Q/aWLznAgb0z+s 8xOD 1.0 eAGbGDGpmvVtgsNn5/+Cx42SHfLz8vLeOe53EPv6gzvowgEHBhCo2u8AAHrkEPs= 1.0 eAGTsK/J1Xod4bD+59mag3mRDikg0BLlsGJdgcP9qGYHBigAAGblDrs= 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7/x8EABTxBfs= eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFjYGRgYGAEAAAMAAM= 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf998jMz0j B4hLUlMU/JOyUpNLiv8DAEYVHsg= eAFjYAABAAAGAAE= 1.0 eAFjYAACRgAABwAC 1.0 eAFzyk+p/J8EIv4xgGBxfk5myn8Af8gLTw== eAFzL0qs/P+PAQIBLrQGiw== 1 eAEdyzEKgDAMBdAb5RLqbMHBUUL7xUA0pU28vorz4003a7CLXSM7U2qWUaKh0wJF 9mQ19Heaw2t4p/UwxQDVvu2ijoZC5cviOJ8X9OIgDA== 1 PopulationSelectPopulation eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAFzyk+p/J8EIv4xgCAARPsINQ== colorset@rainbow body 1 colorset@rainbow Body Body 0 1 1 Filtered Filtered Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation.Outputs.WholeCells_filtered.dataitem 0 UI_in=UI; UI=ocnt(); UI::C_Layout("(1)", vec("Controls"), cellalign="center", class="packer") Buttons=UI; UI=UI_in; 3.0 0 PopulationSelectPopulation 1 AnnexinV_neg cells Select a population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAENzDEKgDAMBdAbZfIG6uCkm6ME/WAhtqX5ETy9Oj94460WylTyoFRZWtlxRINL r7aHKTFlInvi82NFY4JvnczBGnRZz2LoYeZy/EMirvcDmKgh6A== eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf5DwDILg0V eAHjBgAADAAM 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAELTs1JTS4JyC8ozUksyczPizf9DwBMfAgf eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 SelectPopulation 0 1 UI.InputParameters.SelectPopulation.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAFzzMtLrcjMC4vPS01XSE7NySn+7wgTKsgvBgv99ytNzknN/A8AlEMS6Q== Filter by Property Select a method 0 Complex s Method Method Discrete 0 SelectPopulation 0 1 UI.InputParameters.SelectPopulation.Method.title 1 eAFzy8wpSS1SSKpUCCjKL0gtKqn8DwBG/Afm Filter by Property eAFzy8wpSS1SSKpUCCjKL0gtKqn875yfm5ufp+AGlin+75OZl5pYpOCck1hcnJmW mVr0Pzg1JzW5BKTHN7E4+z8Anq0bzA== 0.0 Define a proper value for the selection criterion 0 Complex d Attribute1 3.00000000000000018e+34 -3.00000000000000018e+34 Numeric 0 SelectPopulation 0 0 UI.InputParameters.SelectPopulation.Method.Attribute1 1 0 130 eAFjYACCB5EOs2YCQWWUgzEICEc7nD0DBGuiHdJAwC3GAaSM4UEMVF0sVF0cVF0c VF08hH4WD1HvkACR70lwAADwdyhS 1.0 2.40600000000000014 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName1, RegionTable=ScalarTable| ScalarTable_1=TableForSelectedRegion, SelectedScalarRowIndex_1=SelectedRegionRowIndex) Complex s AttributeName1 Discrete _Scalar5>130 3 3 eAGLdzeM/x/vbgQijOP/AwAxDQaj 0 SelectPopulation 0 0 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwdXcLUPBNTcz7DwALYA4K eAELTk7MSSz6DwALOQNW eAGLD05OzEksMv0PABFSA+o= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf5DwDILg0V eAEzNPwPAAH3AWI= eAH7DwABAAEA eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwdXcLUPBNTcz7DwALYA4K eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cHV3C/gPAO0OExM= eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAFzdXcL+A8ABOsCIg== eAEz+A8AAWEBMA== eAFzzi/NKynWD8isSM35DwAjwwWt eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cHV3C/gPAO0OExM= eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjYWBgAAAAFAAF 1.0 5.0 UI.InputParameters.SelectPopulation.Method.Attribute1.label 1 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwdXcLUPBNTcz7DwALYA4K Intensity Cytoplasm Region EGFP Mean eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP0/BMSe1IlHBzMQcotIxLy+1IjMvLD4vNV0hOTUnp/g/XKggvxgs hM84V3e3AIhJAI6UOtg= > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier1 Discrete 0 SelectPopulation 0 0 UI.InputParameters.SelectPopulation.Method.Attribute1.qualifier 1 eAGz+w8AAX0BPg== > eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB 0.0 Define a proper value for the selection criterion 0 Complex d Attribute2 Numeric 0 SelectPopulation 0 0 UI.InputParameters.SelectPopulation.Method.Attribute2 1 0 0 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName2, RegionTable=ScalarTable| ScalarTable_2=TableForSelectedRegion, SelectedScalarRowIndex_2=SelectedRegionRowIndex) Complex s AttributeName2 Discrete 0 SelectPopulation 0 0 eAHzy89L/Q8ABloCkA== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA UI.InputParameters.SelectPopulation.Method.Attribute2.label 1 eAH7DwABAAEA eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP0/BMSe1IlHBzMQcotIxLy+1IjMvLD4vNV0hOTUnp/g/XKggvxgs hM84V3e3AIhJAI6UOtg= > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier2 Discrete 0 SelectPopulation 0 0 UI.InputParameters.SelectPopulation.Method.Attribute2.qualifier 1 eAGz+w8AAX0BPg== > eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB 0.0 Define a proper value for the selection criterion 0 Complex d Attribute3 Numeric 0 SelectPopulation 0 0 UI.InputParameters.SelectPopulation.Method.Attribute3 1 0 0 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName3, RegionTable=ScalarTable| ScalarTable_3=TableForSelectedRegion, SelectedScalarRowIndex_3=SelectedRegionRowIndex) Complex s AttributeName3 Discrete 0 SelectPopulation 0 0 eAHzy89L/Q8ABloCkA== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA UI.InputParameters.SelectPopulation.Method.Attribute3.label 1 eAH7DwABAAEA eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP0/BMSe1IlHBzMQcotIxLy+1IjMvLD4vNV0hOTUnp/g/XKggvxgs hM84V3e3AIhJAI6UOtg= > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier3 Discrete 0 SelectPopulation 0 0 UI.InputParameters.SelectPopulation.Method.Attribute3.qualifier 1 eAGz+w8AAX0BPg== > eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB Nuclei Selected Name of the output population. 0 Complex s OutputName Output Population Numeric 0 Nuclei Selected Nuclei Selected 1 0 SelectPopulation 0 1 UI.InputParameters.SelectPopulation.OutputName 1 0 Transfected cell 124082 124082 4.0 1 eAELKUrMK05LTS5JTVFITs3J+Q8APKIHMw== 4.0 5.0.1.124082 0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAELSk3PzM/7/48BAgE6agdc eAHzK03OSS0t/h8flJqemZ9nBGMYwxgmMIbpfwC2LBO1 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFzy8xL8StNzknN/O8GZDpXluQX5CQW5/7/x/iPMTg1JzW5xDk1JycoNT0zP+8/ ANRdFF4= eAEz+W/6/x/jP0az/wAfCQWb eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAH775yak6OQVKmQnJGYl5eao+CcmJNcmpNYkpqi4JmbmJ6qo5CbWpKRn6Lg9B+i NL8oJbWIWB2VJfkFOYnFuUSq/w8AB+c1/g== eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmfZ636HwBf fxbe eAH7/x8IAA72BPw= eAHzyE9NziguUfBNLM7+75yYk1yak1iSmqLgmZuYnvr/H+M/xv8AAJwPaw== eAEz+P+PAQIBHrkFKA== eAH7/x8IAA72BPw= eAH7/x8IAA72BPw= eAH7/x8IAA72BPw= eAFL+f+PAQIBIMEFXA== eAH7/x8IAA72BPw= 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eAH7////f4/81OSM4hIF38TibCD3HwMQQSCYAyKAYkAAAICrHVo= eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA eAELKUrMzMvMS3dJLEn8DwAkdQW2 1 Method MethodFolder InputParameters.SelectPopulation.Method Method WholeCells_string Method 1 1 0 1 builtin Each SelectPopulation_2 1 Replace me 1 1 Transfected cell PopulationSelectPopulation_2 Population: Transfected cell 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAELTs1JTS5JTVHwT8oCMor/u2QWJycWpSCJAAD5nw5/ Standard 0 eAELKMovSC0qyUwtVggpSswrTktNLklNUUhOzcn5DwCgoQuA FeedBack.PopulationSelectPopulation_2 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PAQIBLrQGiw== eAFzL0qs/P+PAQIBLrQGiw== eAFjZAACAAAKAAI= 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjYAACAAAFAAE= 1.0 eAEBKADX/xncJjMr4eE/Ajrv2Vby4D8LmwbjddPUP3TbM1GnqNU/36bhKLWp1D+T +xUF 1.0 eAFb2sx9bMvrBAe9HaF6O64mOdzpSlPwDtzlsLLn1Aqf1j0ODCBQtcsBAIaaETs= 1.0 eAHb+uNF8TSNSIfF/SGL+69EOvz1iH1mvjbaYdes1TtnXW12YIACAK85EXY= 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7/x8MABvrBvo= eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFjYAACRkYAAAoAAw== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9z84NSc1 uSQ1RcE/KQvIKP7vklmcnFiUgiQCAEB4JPw= eAFjYAADAAAHAAE= 1.0 eAFjYAADAAAHAAE= 1.0 eAFzyk+p/P+PAQKTwBxGAGo0CjI= eAFzL0qs/P+PAQYBPcYHiQ== 1 eAEdzDEKgDAMQNEbZfAMKjhZcHAsoQ1YiKY0idfX0vl9/vIiO1qRZ0ZDCE0SZW+k cBBTsiDVeXicYHerbgrnJd2IWeMqbWN2tTYiyP1SjO7vBxxSI58= eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFjYAACRkYAAAoAAw== 1.0 eAFzyk+p/P+PAQKTwBxGAGo0CjI= green body 1 colorset@rainbow Body colorset@rainbow Body Body 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8V3ZzszQ2cPwPAK5VE2o= eAFjYAACRkZGAAAOAAQ= 1.0 Stencils Render 10.0 eAFzyk+p/P+PAQKTwBzG4vyczJT/ALDvDUw= 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation_2.Outputs.Wholecells_ForIllustration.dataitem 3.0 1 AnnexinV+ Transfected cell FilteredSelectPopulation_2 Population: AnnexinV+ Transfected cell 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzTs3J+Q8ABgMCgA== Standard 0 eAELKMovSC0qyUwtVnDMy0utyMwL01YIKUrMK05LTS5JTVFITs3J+Q8AGvYO8g== FeedBack.FilteredSelectPopulation_2 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PAQIBLrQGiw== eAFzL0qs/P+PAQIBLrQGiw== eAFjZAACAAAKAAI= 1.0 eAFzzEmtSFQwMzH/7+ruFvDfw9/V2SM4RMHY2NjE6L9zYk5yaU5iSWqKgmduYnrq f4/81OSM4hIF38Ti7P8AeVEWZg== eAFjYAACAAAFAAE= 1.0 eAEBKADX/xncJjMr4eE/Ajrv2Vby4D8LmwbjddPUP3TbM1GnqNU/36bhKLWp1D+T +xUF 1.0 eAFb2sx9bMvrBAe9HaF6O64mOdzpSlPwDtzlsLLn1Aqf1j0ODCBQtcsBAIaaETs= 1.0 eAHb+uNF8TSNSIfF/SGL+69EOvz1iH1mvjbaYdes1TtnXW12YIACAK85EXY= 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7/x8IAA72BPw= eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFjYAACAAAFAAE= 1.0 eAFjYAACAAAFAAE= 1.0 eAFzyk+p/J8EIv4xgCAARPsINQ== eAFzL0qs/P+PAQYBPcYHiQ== 1 eAEdyzEKgDAMBdAbZfAK6mzBwbGE9ouFaEqbeH0V58ebbxZnK3pNbEyhaUL2hk4r BMmCVpff40CLW3XrtB0qGCHS417E0JApf70YzucFLJMgnQ== 1 PopulationSelectPopulation_2 eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAFzyk+p/J8EIv4xgCAARPsINQ== colorset@rainbow body 1 colorset@rainbow Body Body 0 1 1 Filtered Filtered Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation_2.Outputs.WholeCells_filtered.dataitem 3.0 0 PopulationSelectPopulation_2 1 Nuclei Select a population 0 Complex s WholeCells_string Population Discrete eAELKUrMK05LTS5JTVFITs3J+Q8APKIHMw== eAELz8jPSXVOzckpjk/LzClJLUpN+Q8AU8kIoA== eAHzK03OSc38DwALhgNg eAEdyzEKgDAMBdAb5RLqbMHBUUL7xUA0pU28vorz4003a7CLXSM7U2qWUaKh0wJF 9mQ19Heaw2t4p/UwxQDVvu2ijoZC5cviOJ8X9OIgDA== eAFjBAAAAgAC 1.0 eAELTs1JTS4JyC8ozUksyczP+w8APd0Hiw== eAELTs1JTS4JyC8ozUksyczP+w8APd0Hiw== eAHjAQAADQAN 1.0 eAFjAAAAAQAB 1.0 eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELTs1JTS4JyC8ozUksyczP+w8APd0Hiw== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA eAHzT8pKTS4p/g8ADnADyg== 0 SelectPopulation_2 0 1 UI.InputParameters.SelectPopulation_2.WholeCells_string 1 eAELKUrMK05LTS5JTVFITs3J+Q8APKIHMw== Transfected cell eAHzK03OSc3875iXl1qRmRcWn5earpCcmpNTjBAqyC8GC/0PKUrMK05LTS5JTYEI AAAiZxob Filter by Property Select a method 0 Complex s Method Method Discrete 0 SelectPopulation_2 0 1 UI.InputParameters.SelectPopulation_2.Method.title 1 eAFzy8wpSS1SSKpUCCjKL0gtKqn8DwBG/Afm Filter by Property eAFzy8wpSS1SSKpUCCjKL0gtKqn875yfm5ufp+AGlin+75OZl5pYpOCck1hcnJmW mVr0Pzg1JzW5BKTHN7E4+z8Anq0bzA== 0.0 Define a proper value for the selection criterion 0 Complex d Attribute1 3.00000000000000018e+34 -3.00000000000000018e+34 Numeric 0 SelectPopulation_2 0 0 UI.InputParameters.SelectPopulation_2.Method.Attribute1 1 0 120 eAFjYDBg8GBIYKhg6GFYBYQfGAAdFgQh 1.0 24.0 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName1, RegionTable=ScalarTable| ScalarTable_1=TableForSelectedRegion, SelectedScalarRowIndex_1=SelectedRegionRowIndex) Complex s AttributeName1 Discrete _Scalar2>=120 3 3 eAGLdzeM/x/vbgQijOP/AwAxDQaj 0 SelectPopulation_2 0 0 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= eAELTk7MSSz6DwALOQNW eAGLD05OzEksMvoPABFMA+c= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf6DwDIKg0T eAGz+A8AAXEBOA== eAH7DwABAAEA eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cMxJrUhUMDMx/w8AT34UnQ== eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAFzzEmtSFQwMzH/DwASlwOs eAEz+A8AAWEBMA== eAFzzi/NKynWD8isSM35DwAjwwWt eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cMxJrUhUMDMx/w8AT34UnQ== eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjZGBgAAAACAAC 1.0 5.0 UI.InputParameters.SelectPopulation_2.Method.Attribute1.label 1 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= Intensity Cytoplasm Region Alexa 647 Mean eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP0/BMSe1IlHBzMQcotIxLy+1IjMvLD4vNV0hOTUnp/g/XKggvxgs hM84V3e3AIhJAI6UOtg= > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier1 Discrete 0 SelectPopulation_2 0 0 UI.InputParameters.SelectPopulation_2.Method.Attribute1.qualifier 1 eAGzs/0PAAI2AXs= >= eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB 0.0 Define a proper value for the selection criterion 0 Complex d Attribute2 Numeric 0 SelectPopulation_2 0 0 UI.InputParameters.SelectPopulation_2.Method.Attribute2 1 0 0 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName2, RegionTable=ScalarTable| ScalarTable_2=TableForSelectedRegion, SelectedScalarRowIndex_2=SelectedRegionRowIndex) Complex s AttributeName2 Discrete 0 SelectPopulation_2 0 0 eAHzy89L/Q8ABloCkA== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA UI.InputParameters.SelectPopulation_2.Method.Attribute2.label 1 eAH7DwABAAEA eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP0/BMSe1IlHBzMQcotIxLy+1IjMvLD4vNV0hOTUnp/g/XKggvxgs hM84V3e3AIhJAI6UOtg= > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier2 Discrete 0 SelectPopulation_2 0 0 UI.InputParameters.SelectPopulation_2.Method.Attribute2.qualifier 1 eAGz+w8AAX0BPg== > eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB 0.0 Define a proper value for the selection criterion 0 Complex d Attribute3 Numeric 0 SelectPopulation_2 0 0 UI.InputParameters.SelectPopulation_2.Method.Attribute3 1 0 0 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName3, RegionTable=ScalarTable| ScalarTable_3=TableForSelectedRegion, SelectedScalarRowIndex_3=SelectedRegionRowIndex) Complex s AttributeName3 Discrete 0 SelectPopulation_2 0 0 eAHzy89L/Q8ABloCkA== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA UI.InputParameters.SelectPopulation_2.Method.Attribute3.label 1 eAH7DwABAAEA eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP0/BMSe1IlHBzMQcotIxLy+1IjMvLD4vNV0hOTUnp/g/XKggvxgs hM84V3e3AIhJAI6UOtg= > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier3 Discrete 0 SelectPopulation_2 0 0 UI.InputParameters.SelectPopulation_2.Method.Attribute3.qualifier 1 eAGz+w8AAX0BPg== > eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB Transfected cell Selected Name of the output population. 0 Complex s OutputName Output Population Numeric 0 Transfected cell Selected Transfected cell Selected 1 0 SelectPopulation_2 0 1 UI.InputParameters.SelectPopulation_2.OutputName 1 0 AnnexinV+ Transfected cell 124082 124082 4.0 1 eAFzzMtLrcjMC9NWCClKzCtOS00uSU1RSE7NyfkPAIvmCqU= 4.0 5.0.1.124082 0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAELSk3PzM/7/48BAgE6agdc eAHzK03OSS0t/h8flJqemZ9nBGMYwxgmMIbpfwC2LBO1 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1.0 eAFjYIADAAALAAE= 1.0 eAGdkk0LwjAMhv9K7uLFDj2PMZ0HP9DiVUqNo9Clw3Sw/XqFiZ5GW73nyfOSN/Kh iO+oPd5Ao7Uwhy15JDZ+gH2nLXYM1aEsqrMEIUS2gB0qesoQVwzetVZxAyesjSPI LfYKltnqH7jcrI9vbsxjUiNOTMeC5UTYG7pcCevxHJwqi5DJ4tbxTz18vZNgqnYG wT4Df/DxRzbEgrwADS/l0A== eAH7/x8OAEHJCvY= eAELTs1JTS4JyC8ozUksyczP+/+P4R+DW2Zeil9pck5q5v9/jMFoKuJN/v9jwhA0 /f+PGUPQ6P8/FgAn6Sq/ eAELTs1JTS4JyC8ozUksyczPizf6/48BEwIAW9kSCA== eAELz8jPSXVOzckp/v+P4R9DfLxfaXJOaqbB/3+M8fEOjnl5qRWZeWHxeanpDkYG ySB1hv//MSFLFeQXQ6WM/v9jDoebF5+WmVOSWpSa8v8fCwCUYCrI eAFjZGRkYmJmZmFhZQUAAJMAIA== 1.0 eAHzT8pKTS4p/v+PARMCAMdcDbY= eAFjYACBD/ZgioHBAUKLQGkFKK0EpfWgtAGUNoLSxlDaDEqbOwAA6dMFNg== 1.0 5.0 1 ReturnResults 1 Replace me 1 1 Results eAElzEkKgDAQBdEb9SniwpXBC0hjvhDIID3k+hpxVdTmLYOLs+XeAhtTlH4iuUBp h7m0HerFlDa322cDrtyQ/o0sXGEQPXIpriZTGqCPWg31eQHlGSXu 0 0 0 Summary 0 1 1 Transfected cell @Transfected@20cellReturnResults Population: Transfected cell 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzTs3J+Q8ABgMCgA== Standard 0 eAELKMovSC0qyUwtVggpSswrTktNLklNUUhOzcn5DwCgoQuA FeedBack.@Transfected@20cellReturnResults eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQBAAAbAAc= 1.0 eAFzL0qs/P+PAQYBPcYHiQ== eAFzL0qs/P+PAQYBPcYHiQ== eAFjZAABAAAMAAI= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYAABAAAGAAE= 1.0 eAEBMADP/9yQEDE03ck/MzTH1vpv0z+qd3R0egLRPzYI6/YnTdU/z3RljZhM1j9S t3PGn7fTP0biGFo= 1.0 eAFj395XVVC6zEH1acOJAu8VDtv8pi/75TXFQfHjW4WPJ+c57A87z9eYt86BAQQs FjgAAECoFX4= 1.0 eAHjltdc9yEw0sE3+vH7ZxOjHBaJdE8LDYh0+PhW4ePbBzEObEsPHfkd2uzAAAUA ItsUGA== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8EABTxBfs= eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFjYGRgYGAEAAAMAAM= 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf998jMz0j B4hLUlMU/JOyUpNLiv8DAEYVHsg= eAFjYAABAAAGAAE= 1.0 eAFjYAACRgAABwAC 1.0 eAFzyk+p/J8EIv4xgGBxfk5myn8Af8gLTw== eAFzL0qs/P+PAQYBPcYHiQ== 1 eAEdyzEKgDAMBdAbZfAK6mzBwVFC+8FCNKVJvL6K8+PNN0uwV70mdqbUNaNEh9EK QfakLeT3faAlvIUbbYcKRogYlW9Vx/m8GS0c7w== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAFzyk+p/J8EIv4xgCAARPsINQ== colorset@rainbow body 1 colorset@rainbow Body Body 0 1 1 @Transfected@20cell @Transfected@20cell Images=AAL::GetValidImages(ScaleLevel=0).v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation_2.Outputs.WholeCells.dataitem 0 UI_in=UI; UI=ocnt(); UI::C_Layout("(1)", vec("Controls"), cellalign="center", class="packer") Buttons=UI; UI=UI_in; 3.0 1 Nuclei NucleiReturnResults Population: Nuclei 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzTs3J+Q8ABgMCgA== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.NucleiReturnResults eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQBAAAbAAc= 1.0 eAFzL0qs/P+PAQYBPcYHiQ== eAFzL0qs/P+PAQYBPcYHiQ== eAFjZAABAAAMAAI= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYAABAAAGAAE= 1.0 eAEBMADP/9yQEDE03ck/MzTH1vpv0z+qd3R0egLRPzYI6/YnTdU/z3RljZhM1j9S t3PGn7fTP0biGFo= 1.0 eAFj395XVVC6zEH1acOJAu8VDtv8pi/75TXFQfHjW4WPJ+c57A87z9eYt86BAQQs FjgAAECoFX4= 1.0 eAHjltdc9yEw0sE3+vH7ZxOjHBaJdE8LDYh0+PhW4ePbBzEObEsPHfkd2uzAAAUA ItsUGA== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8EABTxBfs= eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFjYGRgYGAEAAAMAAM= 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf998jMz0j B4hLUlMU/JOyUpNLiv8DAEYVHsg= eAFjYAABAAAGAAE= 1.0 eAFjYAACRgAABwAC 1.0 eAFzyk+p/J8EIv4x/GNwyi9KSS36X5yfk5nyHwC7XQ2u eAFzL0qs/P+PAQYBPcYHiQ== 1 eAEdy0EKgEAIAMAfSfSGutZCDxBxPQiWy672/YrOw6w3WVKoXwsFQenOUrPLgENM OIq3tN9xhj2jZQxA3JJNdIL6JQ05nxf55BxL eAFLzs/JLypOLXEoSszMS8ov//+PAQEBxOQNkQ== eAFjYGQAAQAADQAC 1.0 eAFzyk+p/J8EIv4xwCAAa8MKMQ== colorset@rainbow body 1 colorset@rainbow Body Body 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFjYGRgYGAEAAAMAAM= 1.0 Stencils Render 10.0 eAFzyk+p/J8EIv4x/GNwyi9KSS36X5yfk5nyHwC7XQ2u 1 1 Nuclei Nuclei Images=AAL::GetValidImages(ScaleLevel=0).v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation_2.Outputs.__Nuclei0.dataitem 0 UI_in=UI; UI=ocnt(); UI::C_Layout("(1)", vec("Controls"), cellalign="center", class="packer") Buttons=UI; UI=UI_in; 3.0 1 AnnexinV_neg cells @AnnexinV_neg@20cellsReturnResults Population: AnnexinV_neg cells 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzTs3J+Q8ABgMCgA== Standard 0 eAELKMovSC0qyUwtVnDMy0utyMwLi89LTVdITs3JKf4PALZnDEA= FeedBack.@AnnexinV_neg@20cellsReturnResults eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1