https://github.com/Tanentzapf-Lab/LiveImaging_HematopiesisKinetics_Infection_Ho_Carr
Tip revision: d9c8523c1660ea3fd4263074fc9262389b9105cc authored by Tanentzapf-Lab on 04 February 2023, 19:26:02 UTC
Update README.md
Update README.md
Tip revision: d9c8523
runHeatMap.m
clear all; close all %% Clear workspace
dimIn = inputdlg('Heatmap Dimensions (Ex: 5)');
dim = str2double(dimIn); %% Dimension of heatmap
scale = 1/dim; %% Convert range multiplier
folder = uigetdir;
files = dir([folder '\*.tif']);
list = {'Left', 'Right'};
for i=1: length(files)
image = imread(fullfile([folder '\' files(i).name]));
% Rotate
figure(1)
rectIm = imshow(image);
sz = size(image);
pos = [(sz(2)/4) + 0.5, (sz(1)/4) + 0.5, sz(2)/2, sz(1)/2];
rectrot = drawrectangle('Rotatable', true, ...
'DrawingArea', 'unlimited', 'Position', pos, 'FaceAlpha', 0);
rectrot.Label = 'Rotate Rectangle to Rotate Image';
addlistener(rectrot,'MovingROI', @(src,evt) rotateImage(src, evt, ...
rectIm, image));
% Hold for Rotation
keyPress = 0;
while (keyPress ~= 1)
pause;
keyPress = get(gcf, 'CurrentKey');
if strcmp(keyPress, 'return')
keyPress = 1;
else
keyPress = 0;
end
end
imageR = getimage(rectIm);
close()
% Flip left/right
figure(2)
imshow(imageR)
[align, ~] = listdlg('PromptString',...
'Align Axis (Default: Left)','ListString', list);
if(align == 2)
imageR = flip(imageR, 2);
end
close()
% Pick out the LG and event
figure(3)
imshow(imageR)
select = roipoly();
[x,y] = getpts();
[minx, maxx, miny, maxy] = rangeSelect(select);
rangex = maxx - minx;
rangey = maxy - miny;
xlims = (minx:(rangex*scale):maxx);
ylims = (miny:(rangey*scale):maxy);
matx = -1;
maty = -1;
for j = 1: length(xlims)
if (x > xlims(j))
matx = j;
end
end
for k = 1: length(ylims)
if (y > ylims(k))
maty = k;
end
end
matrix = zeros(dim);
matrix(maty, matx) = 1;
name = strcat(files(i).name, ".mat");
save(name, "matrix");
close()
end