LOCUS       Exported                 187 bp ds-DNA     linear   UNA 30-JUN-2020
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 187)
  AUTHORS   Haapaniemi lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 15, 2020 from SnapGene 5.1.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..187
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..65
                     /label=P7 Adapters
     misc_feature    25..32
                     /label=Read-1 Index (Barcode)
     primer_bind     complement(33..66)
                     /label=Index-1 (custom)
     primer_bind     33..66
                     /label=Read-2 (custom)
     misc_feature    44..93
                     /label=Nuclease_off_-_GSP2
     gap             95..109
                     /label=insert
                     /estimated_length=15
     misc_feature    complement(110..187)
                     /label=Miseq Y Adapter
     primer_bind     complement(110..142)
                     /label=PE Read 1 Sequencing Primer (standard)
     primer_bind     110..142
                     /label=Index-2 (standard)
     primer_bind     111..122
                     /label=Miseq common Adapter
                     /note="NOTE:actually sequence is 5'-[Phos]GATC GAGA 
                     GC*C*A-3' the last *C*A is not complementary to the ""Miseq
                     Y Adapter"" to avoid elongation !!!Just used for ligation 
                     !!!"
     misc_feature    143..150
                     /label=custom UMI
     misc_feature    151..158
                     /label=Read-2 Index (Barcode)
     misc_feature    complement(159..187)
                     /label=P5_2
     misc_feature    complement(162..187)
                     /label=P5_1
ORIGIN
        1 caagcagaag acggcatacg agatbbbbbb bbgtgactgg agtcctctct atgggcagtc
       61 ggtgatttga gttgtcatat gttaataacg gtatnnnnnn nnnnnnnnna gatcggaaga
      121 gcgtcgtgta gggaaagagt gtnnwnnwnn bbbbbbbbgt gtagatctcg gtggtcgccg
      181 tatcatt
//