#!/usr/bin/perl -w
#--------------------------------------------------------------------------#
# Author: Meng Wu, the Gordon Lab, Washington University in St. Louis #
# #
# Usage: #
# see Usage in the code #
# #
# Contact: mengwu@wustl.edu #
#--------------------------------------------------------------------------#
if (!@ARGV){
print "Usage: perl $0 <the output files from the analysis pipeline, the mapped insertions binned to each gene>\n";
exit;
}
my @experiment=@ARGV;
my $sample;
my @sample;
my $ratio;
my $insertion;
foreach my $file(@experiment){
chomp $file;
print "Get the files\n";
if ($file=~m/(.*)\_filter\_cpm\.txt/){
$sample=$1;
push @sample,$sample;
open FILE,$file;
<FILE>;
while (<FILE>){
chomp;
@temp=split /\t/,$_;
$ratio->{$temp[0]}->{$sample}=$temp[2];
$insertion->{$temp[0]}->{$sample}=$temp[1];
}
}
}
my $out1="summary_reads.txt";
my $out2="summary_insertions.txt";
open OUT1,">$out1";
open OUT2,">$out2";
sort @sample;
my @header;
push @header,"Gene";
foreach my $sample (@sample){
# if (defined $candidate->{$sample}){
push @header,$sample;
# }
}
my $line=join "\t",@header;
print OUT1 "$line\n";
print OUT2 "$line\n";
shift @header;
foreach my $gene(sort keys %$ratio){
my @out1;
my @out2;
push @out1,$gene;
push @out2,$gene;
foreach my $sample (@sample){
push @out1,$ratio->{$gene}->{$sample};
push @out2,$insertion->{$gene}->{$sample};
}
my $line1=join "\t",@out1;
my $line2=join "\t",@out2;
print OUT1 "$line1\n";
print OUT2 "$line2\n";
}
close OUT1;
close OUT2;