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https://github.com/arjunrajlaboratory/RajLabSeqTools
10 December 2020, 08:13:14 UTC
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  • Snakemake_bulkRNA
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  • config.yaml
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Tip revision: c8b8c79b2ec9c1bd9eb7ced427bb2aec25f19506 authored by Benjamin Emert on 26 March 2020, 17:37:11 UTC
Updated reorganizeBasespaceFiles.py to better parse samples with same first index (e.g. sample 1 and sample10)
Tip revision: c8b8c79
config.yaml
# path or URL to sample sheet (TSV format, columns: sample, condition, ...)
samples: samples.tsv
# path or URL to sequencing unit sheet (TSV format, columns: sample, unit, fq1, fq2, 
# strandedness). Units are technical replicates (e.g. lanes, or resequencing of the 
# same biological sample).If the column "strandedness" is present (which is optional), 
# can be empty or has one of these values: none, yes or reverse. none is for unstranded 
# protocols, yes an reverse follow the nomenclature used in `htseq-count --reverse` 
# which is referenced in STAR manual section 7, "Counting number of reads per gene".

units: units.tsv

trimming:
  # skip trimming: false or true
  skip: true
  # the sequencing adapter
  adapter: ACGGATCGATCGATCGATCGAT

ref:
  # the STAR index
  index: "refs/hg38_STAR"
  # gtf file with transcripts
  annotation: "refs/hg38_STAR/hg38.gtf"

pca:
  labels:
    # columns of sample sheet to use for PCA
    - condition

diffexp:
  # contrasts for the deseq2 results method
  contrasts:
    infected-vs-mock:
      - infected
      - mock

params:
  star: ""
  cutadapt-se: ""
  cutadapt-pe: ""
  diffgenes: "20"
  log-sig: "-30"
  log2-fold: "2"

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