https://github.com/arjunrajlaboratory/RajLabSeqTools
Tip revision: c8b8c79b2ec9c1bd9eb7ced427bb2aec25f19506 authored by Benjamin Emert on 26 March 2020, 17:37:11 UTC
Updated reorganizeBasespaceFiles.py to better parse samples with same first index (e.g. sample 1 and sample10)
Updated reorganizeBasespaceFiles.py to better parse samples with same first index (e.g. sample 1 and sample10)
Tip revision: c8b8c79
config.yaml
# path or URL to sample sheet (TSV format, columns: sample, condition, ...)
samples: samples.tsv
# path or URL to sequencing unit sheet (TSV format, columns: sample, unit, fq1, fq2,
# strandedness). Units are technical replicates (e.g. lanes, or resequencing of the
# same biological sample).If the column "strandedness" is present (which is optional),
# can be empty or has one of these values: none, yes or reverse. none is for unstranded
# protocols, yes an reverse follow the nomenclature used in `htseq-count --reverse`
# which is referenced in STAR manual section 7, "Counting number of reads per gene".
units: units.tsv
trimming:
# skip trimming: false or true
skip: true
# the sequencing adapter
adapter: ACGGATCGATCGATCGATCGAT
ref:
# the STAR index
index: "refs/hg38_STAR"
# gtf file with transcripts
annotation: "refs/hg38_STAR/hg38.gtf"
pca:
labels:
# columns of sample sheet to use for PCA
- condition
diffexp:
# contrasts for the deseq2 results method
contrasts:
infected-vs-mock:
- infected
- mock
params:
star: ""
cutadapt-se: ""
cutadapt-pe: ""
diffgenes: "20"
log-sig: "-30"
log2-fold: "2"