// Acapella Assay Language / Image Analysis // 2021-09-07 12:58:22 +0200 // AcapellaVersion: 5.0.1.124082 #require AcapellaAssayLanguage #namespace AAL AAL::GetData() AAL::CalculateImage() AAL::CalculateImage() AAL::FindNuclei() AAL::FindCytoplasm() AAL::SelectCellRegion() AAL::CalculateIntensityProperties() AAL::CalculateIntensityProperties() AAL::CalculateProperties() AAL::CalculateIntensityProperties() AAL::SelectPopulation() AAL::SelectPopulation() AAL::FindSpots() AAL::CalculateProperties() AAL::SelectPopulation() AAL::SelectPopulation() AAL::SelectPopulation() AAL::SelectPopulation() AAL::SelectPopulation() AAL::SelectPopulation() AAL::ReturnResults() #begin:attachment(encoding="eventxml",name="EvaluationData") 1 1 1 builtin 1 Each GetData 1 1 Using Individual Planes, FFC None 1 eAELTi0pycxLL/4fkFOanplX/B8ARG8IMg== None 1 0 EACH 0 1 builtin Each CalculateImage_2 1 Replace me 1 1 Calculated Image NaNsCalculateImage_2 Output image: Calculated Image 0 sticky eAFzTsxJLs1JLElNUfDMTUxP/Q8AOewG9Q== eAHzS01PLMksS/0fmpeSmpaZl5ryHwBSSQjE Standard 0 eAFzTsxJLs1JLElNUfDMTUxP/Q8AOewG9Q== FeedBack.NaNsCalculateImage_2 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjAAAAAQAB 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 eAFjAAAAAQAB 1.0 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 eAFjAAAAAQAB 1.0 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 eAFjAAAAAQAB 1.0 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 eAFjAAAAAQAB 1.0 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQCAAAUAAY= 1.0 eAFzL0qs/P+PAQIBLrQGiw== eAFzL0qs/P+PAQIBLrQGiw== eAFjYGBgYAQAAAYAAg== 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/wADmRPe eAFjYAACAAAFAAE= 1.0 eAEBKADX/3ZxJp974NA/X5hOH1ujyz/X1fQ5YjPPP4B/PIkGS9E/YK1knGGW0D+N 8xM3 1.0 eAEBKADX/4x3lv9kj5BAMZEeqJxMsUCkiEMn7Y+MQCk4LX6N9JxA+AUHzLP8sECL MBNz 1.0 eAEBKADX/zeHmAawiVlAlbNb+NXYWkDluuPFlgBZQHJ9LB0oN1xAfKCPP8OUc0CQ IRKu 1.0 eAFjYACBD/ZgCg8NAGlzBew= 1.0 eAFjYACBB/ZgCg8NAGPTBZw= 1.0 eAFjYCAOAAAAKAAB 1.0 11 Regions eAH7/x8AAv8B/w== eAELSk35X5mak5Nf/h8AH7IFtg== eAELSk35X5mak5Nf/h8AH7IFtg== eAFjZAQAAAUAAw== 1.0 eAHzS01PLMksS/0fmpeSmpaZl5ryHwBSSQjE eAFjYAAAAAIAAQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFLTs0rSS36/48BABUgBH8= eAFzL0qs/P+PAQIBLrQGiw== 1 eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v4HAGoSGjo= eAELSk35X5mak5Nf/h8AH7IFtg== eAFjZAQAAAUAAw== 1.0 eAFLTs0rSS36/48BABUgBH8= Red center 1 colorset@rainbow Border colorset@rainbow Border Border 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAELSk35X5mak5Nf/h8AH7IFtg== eAFjZAQAAAUAAw== 1.0 Stencils Render 10.0 eAFLTs0rSS36/48BABUgBH8= 1 1 NaNs NaNs Images=AAL::GetValidImages().v_images; Set(Stencils=AAL::GetImageViewStencilsMultiList2(vec("EvaluationData.Procedures.CalculateImage_2.Outputs.Negativeillustration.dataitem","EvaluationData.Procedures.CalculateImage_2.Outputs.NaNillustration.dataitem"), vec("Negative","Undefined")).v_stencils) Standard EvaluationData.Procedures.CalculateImage_2.Outputs.Negativeillustration.dataitem 3.0 0 NaNsCalculateImage_2 1 By Formula Select a method 0 Complex s Method Method Discrete A+2*C Set to Local Average Set to Zero 0 CalculateImage_2 0 1 UI.InputParameters.CalculateImage_2.Method.title 1 eAFzqlRwyy/KLc1J/A8AF5oEsQ== By Formula eAFzqlRwyy/KLc1J/A8AF5oEsQ== A+B Type a formula in style A+B 0 Complex s sFormula Formula Numeric 0 CalculateImage_2 0 1 UI.InputParameters.CalculateImage_2.Method.sFormula 1 0 A+2*C Alexa 647 Select channel A 0 Complex s 1 1 sChannelA Channel A Discrete 1 CalculateImage_2 0 1 UI.InputParameters.CalculateImage_2.Method.sChannelA 1 eAFLKQ5KTfkPAAjbAvI= dsRed eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X8A7hYM6g== Alexa 647 Select channel C 0 Complex s 1 1 sChannelC Channel C Discrete 1 CalculateImage_2 0 1 UI.InputParameters.CalculateImage_2.Method.sChannelC 1 eAFzzEmtSFQwMzH/DwASlwOs Alexa 647 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X8A7hYM6g== Set to Zero Select channel C 0 Complex s sNegativeMethod Negative Values Discrete 0 CalculateImage_2 0 1 UI.InputParameters.CalculateImage_2.Method.More.sNegativeMethod 1 eAELTi1RKMlXiEotyv8PABvhBO8= Set to Zero eAELTi1RKMlXiEotyv8fnJGZVqLgmVeSmlecWZKZWvzfOzW14D8A+1EPHw== Set to Local Average Select channel C 0 Complex s sNanMethod Undefined Values Discrete 0 CalculateImage_2 0 1 UI.InputParameters.CalculateImage_2.Method.More.sNanMethod 1 eAELTi1RKMlX8MlPTsxRcCxLLUpMT/0PAFB2CBU= Set to Local Average eAELTi1RKMlX8MlPTsxRcCxLLUpMT/0fDBGMSi3K/w8AzUcNAw== Calculated Image Name of output Image 0 Complex s sOutputName Output Image Numeric 0 CalculateImage_2 0 1 UI.InputParameters.CalculateImage_2.sOutputName 1 0 Calculated Image 124082 122842 2 1 2 5.0.1.124082 0 1 eAFz/O/8HwAFigKD Output Image Calculated Image eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+AwB9YAoX eAEr8cxNTE/9DwALjANX eAH7DwABAAEA eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v4HAGoSGjo= eAFjAAAAAQAB 1.0 eAFzTsxJLs1JLEn1zE1MT403+g8AOzMHAg== eAFzTsxJLs1JLEn1zE1MT403+g8AOzMHAg== eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzTsxJLs1JLEn1zE1MT403+g8AOzMHAg== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA eAFzTsxJLs1JLElNUfDMTUxP/Q8AOewG9Q== eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv8Bwh0MPg== eAH7DwABAAEA eAFjBAAAAgAC 1.0 eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdaLjwdJgUWLHeCqUhyMDMBieilAgzJLUnP/AwBWeyMh eAFjBAAAAgAC 1.0 eAFzTsxJLs1JLElNUfDMTUxP/Q8AOewG9Q== eAFzTsxJLs1JLEn1zE1MT403+g8AOzMHAg== eAFjAgAAAwAD 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAGLj/dLTU8sySxL9S9LLcpJrEwtdk7MSS7NSSxJ9cxNTE+NN/ofH++XmIdbGgCR rBtD eAHzS01PLMksS83MySktLikCsvPz/vsl+qHwASuwEIM= eAHzS01PLMksS/XMySktLikCsvPz/vsl5qHwASgwEGM= eAGdzMENgCAMBdCNenAF5eAF3cD8SENIihhoXV+JZ08O8J67IAZN5ZigoLWWnYNV bjRCdhMozxmRt4EW09O0kefYwcVJxJrWF1PoOinn2/0I4b+vB/2PPkk= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PAQBLMwgA eAFzTsxJLs1JLEn1zE1MT403+v+PAQBLMwgA eAFjYgIAAAgABQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PAQBLMwgA eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== 1 Method MethodFolder InputParameters.CalculateImage_2.Method Method __BeforeFirstInput Method 1 1 0 MoreFolder InputParameters.CalculateImage_2.Method.More sChannelC 0 0 0 1 builtin Each CalculateImage 1 Replace me 1 1 Hoechst Mask NaNsCalculateImage Output image: Hoechst Mask 0 sticky eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzS01PLMksS/0fmpeSmpaZl5ryHwBSSQjE Standard 0 eAHzyE9NziguUfBNLM7+DwAifQV6 FeedBack.NaNsCalculateImage eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjAAAAAQAB 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 eAFjAAAAAQAB 1.0 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 eAFjAAAAAQAB 1.0 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 eAFjAAAAAQAB 1.0 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 eAFjAAAAAQAB 1.0 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQBAAAbAAc= 1.0 eAFzL0qs/P+PAQYBPcYHiQ== eAFzL0qs/P+PAQYBPcYHiQ== eAFjYGBgZGAEAAAKAAM= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYAABAAAGAAE= 1.0 eAEBMADP/9QQmgn6wtU/CBhPMeXgyT8YGP5f7YXhP7gsJsyntdc/1mFmKpIQzT8+ YNPo6hTIP0TUF/8= 1.0 eAGz3TCDXUy6zmHOuSNS8xs2O7xrUTmzlCnRoe30zle5T5sdnGKmbT6XutmBAQQ2 tDsAABoIFQ0= 1.0 eAHjtZ/7cPnkSIdfEWf8LG9EOWybeFQ+9mqEw4XFhqsmhMQ47Co36dV0y3JggAIA Iz8Sgw== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8AAv8B/w== eAELSk35X5mak5Nf/h8AH7IFtg== eAELSk35X5mak5Nf/h8AH7IFtg== eAFjZAQAAAUAAw== 1.0 eAHzS01PLMksS/0fmpeSmpaZl5ryHwBSSQjE eAFjYAAAAAIAAQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFLTs0rSS36/48BABUgBH8= eAFzL0qs/P+PAQYBPcYHiQ== 1 eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxP1fMvLSko LSnWK4FwU4CqM0tSc/8DADwBGak= eAELSk35X5mak5Nf/h8AH7IFtg== eAFjZAQAAAUAAw== 1.0 eAFLTs0rSS36/48BABUgBH8= Red center 1 colorset@rainbow Border colorset@rainbow Border Border 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAELSk35X5mak5Nf/h8AH7IFtg== eAFjZAQAAAUAAw== 1.0 Stencils Render 10.0 eAFLTs0rSS36/48BABUgBH8= 1 1 NaNs NaNs Images=AAL::GetValidImages().v_images; Set(Stencils=AAL::GetImageViewStencilsMultiList2(vec("EvaluationData.Procedures.CalculateImage.Outputs.Negativeillustration.dataitem","EvaluationData.Procedures.CalculateImage.Outputs.NaNillustration.dataitem"), vec("Negative","Undefined")).v_stencils) Standard EvaluationData.Procedures.CalculateImage.Outputs.Negativeillustration.dataitem 3.0 0 NaNsCalculateImage 1 By Formula Select a method 0 Complex s Method Method Discrete iif(A>160, A, 0) Set to Local Average Set to Zero 0 CalculateImage 0 1 UI.InputParameters.CalculateImage.Method.title 1 eAFzqlRwyy/KLc1J/A8AF5oEsQ== By Formula eAFzqlRwyy/KLc1J/A8AF5oEsQ== A+B Type a formula in style A+B 0 Complex s sFormula Formula Numeric 0 CalculateImage 0 1 UI.InputParameters.CalculateImage.Method.sFormula 1 0 iif(A>160, A, 0) Alexa 647 Select channel A 0 Complex s 1 1 sChannelA Channel A Discrete 1 CalculateImage 0 1 UI.InputParameters.CalculateImage.Method.sChannelA 1 eAHz8Hd19ggOUTA2NjYx+g8AHFUELQ== HOECHST 33342 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/wADmRPe Set to Zero Select channel A 0 Complex s sNegativeMethod Negative Values Discrete 0 CalculateImage 0 1 UI.InputParameters.CalculateImage.Method.More.sNegativeMethod 1 eAELTi1RKMlXiEotyv8PABvhBO8= Set to Zero eAELTi1RKMlXiEotyv8fnJGZVqLgmVeSmlecWZKZWvzfOzW14D8A+1EPHw== Set to Local Average Select channel A 0 Complex s sNanMethod Undefined Values Discrete 0 CalculateImage 0 1 UI.InputParameters.CalculateImage.Method.More.sNanMethod 1 eAELTi1RKMlX8MlPTsxRcCxLLUpMT/0PAFB2CBU= Set to Local Average eAELTi1RKMlX8MlPTsxRcCxLLUpMT/0fDBGMSi3K/w8AzUcNAw== Calculated Image Name of output Image 0 Complex s sOutputName Output Image Numeric 0 CalculateImage 0 1 UI.InputParameters.CalculateImage.sOutputName 1 0 Hoechst Mask 124082 122842 2 1 2 5.0.1.124082 0 1 eAFz/A8AAYMBQQ== Output Image Hoechst Mask eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAGLj/dLTU8sySxL9S9LLcpJrEwtdk7MSS7NSSxJ9cxNTE+NN/ofH++XmIdXGr8R eA34DwB38DVj eAHzS01PLMksS83MySktLikCsvPz/vsl+qHw/zH8YwQAc7ESgA== eAHzS01PLMksS/XMySktLikCsvPz/vsl5qHw/zH8YwQAb7ESYA== eAGtzjEOgCAMQNEbdfAKyuCC3sA00BiSIgZarq/ExclFPcB/+aYiK0pI24CCMOfk yGumAj2yU0ahMeJKSweTyq5SwNLagkqBWYvkKwbf6iAUD/MCRPvN+nnt5p7GTmLP e28= eAFjYGBgAAAABAAB 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RgAQ3g9v eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RgAQ3g9v eAFjYmJmBgAAGwAL 1.0 eAFjYGBgAAAABAAB 1.0 eAFjYmZiBgAAHAAL 1.0 eAFjYGBgAAAABAAB 1.0 eAFjZGRkBAAADgAF 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RgAQ3g9v eAH7////fwAJ+gP9 eAH7////fwAJ+gP9 eAFjYGBgAAAABAAB 1.0 eAH7////fwAJ+gP9 1 Method MethodFolder InputParameters.CalculateImage.Method Method __BeforeFirstInput Method 1 1 0 MoreFolder InputParameters.CalculateImage.Method.More sChannelA 0 0 0 1 builtin Each FindNuclei 1 Replace me 1 1 Nuclei NucleiFindNuclei Population: Nuclei 0 sticky eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzK03OSS0t/g8ADvED3w== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.NucleiFindNuclei eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 eAFjAAAAAQAB 1.0 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 eAFjAAAAAQAB 1.0 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 eAFjAAAAAQAB 1.0 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 eAFjAAAAAQAB 1.0 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZGRkYGQEAAAYAAY= 1.0 eAFzL0qs/P+P4R9DeEZmSSqYBQBe6QmL eAFzL0qs/P+PAQYBPcYHiQ== eAFjYGBgZGAAAAAJAAI= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYGBgZGAAAAAJAAI= 1.0 eAEBMADP/9QQmgn6wtU/CBhPMeXgyT8YGP5f7YXhP6QlqqRdqdA/1mFmKpIQzT8+ YNPo6hTIP0M5F+M= 1.0 eAGz3TCDXUy6zmHOuSNS8xs2O7xrUTmzlCnRQaHmw0H2/+0OTjHTNp9L3ezAAAIb 2h0ABPoUHg== 1.0 eAHjtZ/7cPnkSIdfEWf8LG9EOWybeFQ+9mqEQ+uV1Rckc2IcdpWb9Gq6ZTkwQAEA JrQSqg== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7DwABAAEA eAFLzs/JLypOLXEoSszMS8ov/w8AP9wHnQ== eAFLzs/JLypOLXEoSszMS8ov/w8AP9wHnQ== eAFjBAAAAgAC 1.0 eAHzK03OSS0t/g8ADvED3w== eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAFLyi9KSS36DwAMKwN+ eAFzL0qs/P+P4R9DeEZmSSqYBQBe6QmL 1 eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFLzs/JLypOLXEoSszMS8ov/w8AP9wHnQ== eAFjBAAAAgAC 1.0 eAFLyi9KSS36DwAMKwN+ colorset@rainbow border 1 colorset@rainbow Border Border 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov/6/s5mZpbOD4HwCFZAoo eAFjZAQAAAUAAw== 1.0 Stencils Render 10.0 eAFLyi9KSS36X5yfk5nyHwAqrgaY 1 1 Nuclei Nuclei Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.FindNuclei.Outputs.Nuclei.dataitem 3.0 1 Initial Mask InitialMaskFindNuclei Population: Initial Mask 0 sticky eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzzMssyUzMUfBNLM7+DwAilQV2 Standard 0 eAELKMovSC0qyUwtVvDMyyzJTMxR8E0szv4PAHVgCcM= FeedBack.InitialMaskFindNuclei eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 1 SingleDefaultOverLay WeightedAverage script 0 1 eAFjZAQBAAAbAAc= 1.0 eAFzL0qs/P+PAQYBPcYHiQ== eAFjYAACRgAABwAC 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== 1 eAHzzMssyUzMUfBNLM7+DwAilQV2 eAFjBAAAAgAC 1.0 eAErSk35DwAEwgI7 eAFLyk+p/A8ABsgCrg== eAErSk35DwAEwgI7 eAENyksOgDAIBcAbcQo1ceHnCqRlQaTUlIfXV2c988OWDO0+MZjO0YvUHBK0qNc9 i4nSkbgTQasrlG3juKj+XSHt/QBVGxoY eAErSk35DwAEwgI7 eAFjBAAAAgAC 1.0 eAFLyk+p/A8ABsgCrg== red body 1 colorset@rainbow Body Body 0 1 1 InitialMask InitialMask Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.FindNuclei.Outputs.InitialMask.dataitem 3.0 1 Low Contrast Objects LowContrastFindNuclei Population: Low Contrast Objects 0 sticky eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzyS9XcM7PKylKLC5R8E/KSk0uKf4PAFWWCIo= Standard 0 eAELKMovSC0qyUwtVvDJL1dwzs8rKUosLlHwT8pKTS4p/g8AyskM1w== FeedBack.LowContrastFindNuclei eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 1 SingleDefaultOverLay WeightedAverage script 0 1 eAFjZAQBAAAbAAc= 1.0 eAFzL0qs/P+PAQYBPcYHiQ== eAFjYAACRgAABwAC 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== 1 eAHzyS9XcM7PKylKLC5R8E/KSk0uKf4PAFWWCIo= eAFjBAAAAgAC 1.0 eAErz8gsSf0PAAmhAyE= eAFLyi9KSS36DwAMKwN+ eAErz8gsSf0PAAmhAyE= eAENy0sOgCAMBcAb9RJ+Vka8QoUuSpAaeNXjK9tJZnm4OEOtzgymo1mU5E06rVrT 7rGIUnDcjk6bvZNVNO4IZ5Y4KI2lkOv7ARZDHQw= eAErz8gsSf0PAAmhAyE= eAFjBAAAAgAC 1.0 eAFLyi9KSS36DwAMKwN+ white border 1 colorset@rainbow Body Body 0 1 1 LowContrast LowContrast Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.FindNuclei.Outputs.LowContrastObjects.dataitem 3.0 0 NucleiFindNuclei 1 HOECHST 33342 Select channel 0 Complex s 1 0 NucleiDetectionChannel_string Channel Discrete 1 eAHzK03OSc38DwALhgNg FindNuclei CalculateImage 0 0 UI.InputParameters.FindNuclei.NucleiDetectionChannel_string 1 eAHzyE9NziguUfBNLM7+DwAifQV6 Hoechst Mask eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== None Select population for ROI 0 Complex s WholeCells_string ROI Discrete 0 FindNuclei 0 1 UI.InputParameters.FindNuclei.WholeCells_string 1 eAHzy89L/Q8ABloCkA== None eAHzy89L/Q8ABloCkA== Select ROI region 1 Complex s 1 Region Discrete 0 FindNuclei 0 1 UI.InputParameters.FindNuclei.Region 1 eAH7DwABAAEA eAH7DwABAAEA B Select method 0 Complex s NucleiDetectionAlgorithm Method Discrete 0 FindNuclei 0 0 UI.InputParameters.FindNuclei.AdditionalParameters.title 1 eAFz+g8AAYUBQg== B eAFz/O/03/m/738AFTIFEA== 0.4 Threshold tuning for the initial thresholding of nuclei. Range: 0..1.0. Low values shrink the mask and the high ones expand the mask. Adjust by illustration InitialMask. 0 Simple d ThresholdAdjustment Common Threshold 1.0 0.0 Numeric 1 AAL::GoodnessProc_CommonThreshold() eAHzzMssyUzM8U0sznbLzEvxK03OSc38D6GQBAA7mRB3 0 FindNuclei 0 0 0.05 UI.InputParameters.FindNuclei.AdditionalParameters.ThresholdAdjustment 1 1 0 eAGbNRMEdtrPAtMn7Y3B4DKUf9OeAQweQMUf26eBwTOo/Ev7s2dA4A1U3Qd7AK9V IwA= 1.0 0.1 30 Nuclei: Minimum allowed area for nuclei, objects with area less than the limit are removed. Too small values of the parameter usually introduce errors. Unit: image pixel. Adjust by illustration Nuclei. 0 Simple d MinimumNuclearArea Minimum Area 500000000 0 Numeric 0 FindNuclei > 0 0 1 UI.InputParameters.FindNuclei.AdditionalParameters.MinimumNuclearArea 1 0 µm² 15 eAFjZeBiEGGQYzBiCGCoYDjBMIFRgRkAFH4CuA== 1.0 3 7.0 Nuclei: Controls splitting of stuck nuclei. Range: 1..100. With the lower values more objects are split. Adjust by illustration Nuclei. Parameter has only limited control other the splitting process. Adjust by illustration Nuclei. 0 Simple d NuclearSplittingAdjustment Splitting Coefficient 100.0 1.0 Numeric 0 FindNuclei 0 0 0.5 UI.InputParameters.FindNuclei.AdditionalParameters.More.NuclearSplittingAdjustment 1 1 100 eAFjYmZl5+KXBAAAsQBE 1.0 1.0 0.4 Nuclei: Controls the size (border position) of nuclei. Range: 0..~1.0. With the higher values nuclei appear smaller and with the lower values larger. Adjust by illustration Nuclei. 0 Simple d IndividualThresholdAdjustment Individual Threshold 1.0 0.0 Numeric 1 OptimizeITA() NucleiDetectionData.NucleIIllustrations.Nuclei Nuclei: Controls the size (border position) of nuclei. Range: 0..~1.0. With the higher values nuclei appear smaller and with the lower values larger. Adjust by illustration Nuclei. 0 FindNuclei 0 0 0.05 UI.InputParameters.FindNuclei.AdditionalParameters.More.IndividualThresholdAdjustment 1 1 0.3 eAGbNRMEdtrPAtMn7Y3B4DKUf9OeAQweQMUf26eBwTOo/Ev7s2dA4A1U3Qd7AK9V IwA= 1.0 0.1 0.1 Nuclei: Minimum allowed contrast for nuclei, objects with contrast less than the limit are removed. Range: 0..1.0. Adjust by illustration LowContrastObjects and Nuclei. 0 Simple d MinimumNuclearContrast Minimum Contrast 1.0 -1.0 Numeric eAHzyS93zs8rKUosLnHLzEvxK03OSc38D6GQBABAjxCh 0 FindNuclei > 0 0 0.05 UI.InputParameters.FindNuclei.AdditionalParameters.More.MinimumNuclearContrast 1 1 0.11 eAFjYACBB/vBFJCYNRMEVtpD6J32xmBwGMo/CaVvQsUf2wMArp0YhA== 1.0 0.1 13.0 Expected diameter. Smaller values yield more fragmented and smaller objects. Range: 2..40. 0 Complex d NucDiam Diameter 40.0 2.0 Numeric 1 Set(NucDiam=NucleiDetectionData.EstimatedDiam) NucleiDetectionData.Nuclei Expected diameter. Smaller values yield more fragmented and smaller objects. Range: 2..40. 0 FindNuclei 1 0 2.0 UI.InputParameters.FindNuclei.AdditionalParameters.NucDiam 0 0 µm 13 4.0 0.4 Controls splitting of stuck nuclei. Range: 0..1.0. At the value 0.0, splitting is switched off. Higher values return increasingly more splitting events. 0 Complex d SplitTuner Splitting Sensitivity 1.0 0.0 Numeric 0 FindNuclei 1 0 0.05 UI.InputParameters.FindNuclei.AdditionalParameters.SplitTuner 0 1 0.4 0.1 0.4 This is a relative threshold determining how dark objects can be detected. Range: 0..1.0. With smaller values, darker objects are detected. 0 Complex d DynRanThr Common Threshold 1.0 0.0 Numeric 0 FindNuclei 1 0 0.05 UI.InputParameters.FindNuclei.AdditionalParameters.DynRanThr 0 1 0.4 0.1 Nuclei Name of the output population. 0 Complex s OutputName Output Population Numeric 1 Nuclei Nuclei 1 0 FindNuclei 0 1 UI.InputParameters.FindNuclei.OutputName 1 0 Nuclei 124082 122842 7 1 eAHzK03OSc38DwALhgNg 7 5.0.1.124082 0 Output Population Nuclei eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAF9zEEKgCAQRuGreIBWXaFVUHaE4U+GsCaFHI1Ob5to6fZ98Igsb1BfeCl8CR5O A8RlgfJ4YmPqK5FFaHJ70RxUm52wr2Pw6iFmRjo688Up3sbFoBeSmrju7DT9+ALy 2ExY eAHzS01PLMksS83MySktLikCsvPz/vsl+qHw/zH8Y/QrTc5JzfzvmZdZkpmY45tY nP3fJ7/cOT8PqKm4xD8pKzW5pPg/AHepI30= eAHzS01PLMksS/XMySktLikCsvPz/vsl5qHw/zH8Y/QrTc5JzfzvmZdZkpmYo+Cb WJz93ye/XME5Pw+orbhEwT8pKzW5pPg/AMoMI70= eAGtkUsOwjAMRG+UBVcoRapUUm6ATGJVBjdBid0eH6Ii0U0X5bOcxXsazdQjsIJQ DHsQMKcUHXpNmE0F7JRBsBmgx/POdCp3lWws9gUYkZg1S5ph4wtNgsOj/kII9jfX n6stus+LHSh4q46RFssrblhoBW4CCQEfId+2bLxiaONUxVB+ytJdrujKg+8qT6bs yrI= eAFjYGBgYGRgAAAACgAC 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5j+MQEAK4MW Tw== eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5j+MQEAK4MW Tw== eAFjYmJmZmFhAQAAVAAX 1.0 eAFjYAADAAAHAAE= 1.0 eAFjYmZiZmRmAQAASgAT 1.0 eAFjYAADAAAHAAE= 1.0 eAFjZAQDAAAjAAg= 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5j+MQEAK4MW Tw== eAH7////f7/8vFQgBQA0VQiK eAH7/x8MABvrBvo= eAFjYAADAAAHAAE= 1.0 eAH7////f4/81OSM4hIF38TibCAXAHYsC3Q= eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA 1 AdditionalParameters MethodFolder InputParameters.FindNuclei.AdditionalParameters NucleiDetectionAlgorithm Region AdditionalParameters 0 1 0 1 builtin Each FindCytoplasm 1 Replace me 1 1 Nuclei CellsFindCytoplasm Population: Nuclei 0 sticky eAFzTsxJLs1JLElNUfDMTUxP/Q8AOewG9Q== eAFzTs3J+Q8ABgMCgA== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.CellsFindCytoplasm eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 eAFjAAAAAQAB 1.0 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 eAFjAAAAAQAB 1.0 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 eAFjAAAAAQAB 1.0 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 eAFjAAAAAQAB 1.0 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZGAEAgAAFgAG 1.0 eAFzL0qs/P+P4R+DsqGJq5OrE5gNAGXaCSA= eAFzL0qs/P+PAQYBPcYHiQ== eAFjYGBgZGQAAAALAAM= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjZGRgZGQEAAAXAAY= 1.0 eAEBMADP/3ZxJp974NA/cB2p15F6vT/X1fQ5YjPPP4B/PIkGS9E/YK1knGGW0D+Q +5FRaKDIP1WHGFs= 1.0 eAHrKZ/2P6V/gsOyF4u69aRmOyzpcFZ/29/joGmhW9f7ZY7DD1b2M5v/bHBgAIEd kxwAQBkVeg== 1.0 eAEzb5/BtqEz0oHr/Q7z8CeRDk93PT46jSHSoahWR1bDPMahZkG//eEpxQ4MUAAA +rARiA== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7////fwAJ+gP9 eAFLzs/JLypOLXEoSszMS8ov//+PAQQBeXIKlw== eAFLzs/JLypOLXEoSszMS8ov//+PAQQBeXIKlw== eAFjZGRgAAAACwAD 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJx7n8A868PPw== eAFjYGBgAAAABAAB 1.0 eAFjYGBgAAAABAAB 1.0 eAFzyi9KSS36/48BBAErZwZY eAFzL0qs/P+P4R+DsqGJq5OrE5gNAGXaCSA= 1 eAENyzEKgDAMAMAf5RNVV90cJdiAgbQpTVLw9drbbx0ogc5aF3SEo+tNOToZbFxz el2boBXYw1u4wfmoUCIRu1gkzPvMgyDPzU7l+wETViCX eAFLzs/JLypOLXEoSszMS8ov//+PAQQBeXIKlw== eAFjYGRgAAAABwAC 1.0 eAFzyi9KSS36/48BBAErZwZY colorset@rainbow Border 1 colorset@rainbow Border Border 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQSV3dwsjQ0c/wMA1soNIg== eAFjZGRgYAQAAA8ABA== 1.0 Stencils Render 10.0 eAFzyi9KSS36/48BBIvzczJT/gMAWwYJcg== 1 1 Cells Cells Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.FindCytoplasm.Outputs.WholeCells_illustrative.dataitem 3.0 0 CellsFindCytoplasm 1 Alexa 647 Select channel 0 Complex s 1 0 CytoplasmDetectionChannel_string Channel Discrete 1 FindCytoplasm CalculateImage_2 0 0 UI.InputParameters.FindCytoplasm.CytoplasmDetectionChannel_string 1 eAFzTsxJLs1JLElNUfDMTUxP/Q8AOewG9Q== Calculated Image eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== Nuclei Select nuclei population 0 Complex s Nuclei_string Nuclei Discrete eAHzK03OSc38DwALhgNg eAHzK03OSc38DwALhgNg eAHzK03OSc38DwALhgNg eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdaD clOAKjNLUnP/AwDZaxgi eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 FindCytoplasm 0 1 UI.InputParameters.FindCytoplasm.Nuclei_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc38DwALhgNg A Select a method 0 Complex s Method Method Discrete 0 FindCytoplasm 0 0 UI.InputParameters.FindCytoplasm.Method.title 1 eAFz+g8AAYUBQg== B eAFz/O/03/m/y3/X/27/AS7YB5A= Alexa 647 Select membrane stained channel 0 Complex s 1 1 MembraneChannel_string Membrane Channel Discrete 1 FindCytoplasm 1 0 UI.InputParameters.FindCytoplasm.Method.MembraneChannel_string 0 eAFLKQ5KTfkPAAjbAvI= dsRed eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== 0.45 Controls common threshold calculation for cytoplasm detection 0 Simple d CytoplasmThresholdAdjustment Common Threshold 1.0 0.0 Numeric 0 FindCytoplasm 0 0 0.05 UI.InputParameters.FindCytoplasm.Method.CytoplasmThresholdAdjustment 1 1 0.25 0.1 0.15 Controls individual threshold calculation, as result cytoplasm/cell regions can appear larger or smaller 0 Simple d CytoplasmIndividualThresholdAdjustment Individual Threshold 1.0 0.0 Numeric 1 GoodnessProc_CytoplasmIndividualThreshold() 0 FindCytoplasm 0 0 0.05 UI.InputParameters.FindCytoplasm.Method.CytoplasmIndividualThresholdAdjustment 1 1 0.39 eAGbNRMEVtrPAtM77Y3B4DCUfxLKvwzl37RnAIMHUPHH9mlg8Awq/9L+7BkQeANV 98EeABv8KbM= 1.0 0.05 Select ROI region 0 Simple s sROIRegion Restrictive Region Discrete 0 FindCytoplasm 0 1 UI.InputParameters.FindCytoplasm.Method.sROIRegion 1 eAH7DwABAAEA eAH7DwABAAEA 0 Use ROI region 1 Complex b yRoiOnOff Numeric 0 FindCytoplasm 0 1 UI.InputParameters.FindCytoplasm.Method.sROIRegion.qualifier 1 0 0 MultiNucleated Cells Name of the output population 0 Complex s OutputName Output Population Numeric 1 MultiNucleated Cells MultiNucleated Cells 1 0 FindCytoplasm 1 1 UI.InputParameters.FindCytoplasm.OutputName 0 0 MultiNucleated Cells 124082 122842 5 1 eAHzLc0pyfQrTc5JTSxJTVFwTs3JKf4PAFqwCLM= 5 5.0.1.124082 0 Output Regions Cell, Cytoplasm, Membrane eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAF9j0EKwjAQRa+SA7jyCgWhoPEIwzcOZXSaSGdS6emjQnGZ7X+PB58o8gSXla8r L4qNbYCmqnAeZ0xMx0YUkbu4n+gGWqxJWdqYxQUaLrDnIezjubxDKtkXmIdye3By +8PdOUm+D5uXl8JmEtVqX//3qH0AF2Bbeg== eAFVjDEOgCAMAPVtTCaKo6OppNFqA4mt+Hsqm7rdDXceV1DKSMyX6Fk5RfPgf16a 0vorMJJ1kZSAB5DD+nS7FGskOi47BhWbtsTokPmL8zvLaA9cYjLm eAFVyzEOgCAMQFH1JNyFiURxdK6kUbQpiS16e6qr23/Dj7iB5hsDURW9vi5sEfjn 1rU+1kSYLXDWDOQmkNPG8jhf+NtE3bwemFSsDcteCD0Sib3B/Cmu eAGtkTEOwjAMRW+UgSuEIiFBy8ZYmdQqBjepGruI00MEElk6FOjo4T89/1+MwApC wa9BwByG4LDRAaOxwE4ZBLcdtFivTKXSq0RTYpsCIxKzRhleYdOkNAl2j+IHIJT/ sRZWy7jvxTbkm1IdI2XK+5zR0ER460kIeA/xOqfjCcIu3Gzwaaco1emCLi04U8Xe JfQMsfu8cjwHRovMizDqvPqIWeoJ2i4GZg== eAFjYAADRgYAAAsAAg== 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5hAECTgXFmS X5CTWJz7HwDKFx2L eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5j+MYG4zpUl +QU5icW5//8xAwDIkB2M eAFjYmJmZmFhYWUFAACRACE= 1.0 eAFjYIACAAAJAAE= 1.0 eAFjYmZiZmRmYWQCAAB0ABY= 1.0 eAFjYGBgYAViBgAAIgAG 1.0 eAFjZAQDBkYAADQACQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5hAECTgXFmS X5CTWJz7HwDKFx2L eAH7////f7/8vFQg9Y/hPwBR7AqH eAH7/x8KACzcCPg= eAFjYIACAAAJAAE= 1.0 eAH7////f4/81OSM4hIF38TibCD3H8N/AJyBDXE= eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA 1 Method MethodFolder InputParameters.FindCytoplasm.Method Method Nuclei_string Method 0 1 0 1 builtin Each SelectCellRegion 1 Replace me 1 1 Nuclei CellRegionSelectCellRegion The created region Cytoplasm Region. 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.CellRegionSelectCellRegion eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQBAAAbAAc= 1.0 eAFzL0qs/P+PAQYBPcYHiQ== eAFzL0qs/P+PAQYBPcYHiQ== eAFjYGQAAgAACwAC 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYAABAAAGAAE= 1.0 eAEBMADP/3ZxJp974NA/X5hOH1ujyz/X1fQ5YjPPP4B/PIkGS9E/YK1knGGW0D+Q +5FRaKDIPz1hF7M= 1.0 eAHrKZ/2P6V/goPhRLkVc3w2OizpcFZ/29/joGmhW9f7ZY7DD1b2M5v/bHBgAIEd kxwAKmMU/Q== 1.0 eAEzb5/BtqEz0mHq5ugfV29EOTzd9fjoNIZIh6JaHVkN8xiHmgX99oenFDswQAEA JaASrg== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8IAA72BPw= eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGBgYAQAAAYAAg== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFjZAACAAAKAAI= 1.0 eAFjYAACAAAFAAE= 1.0 eAELzs/JTPnvlJ9S+f8fIwgCAEsACIU= eAFzL0qs/P+PAQYBPcYHiQ== 1 eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1gtOzUlNLnFOzckJSk0Hyuv5l5YU lJYU64Vn5OekgsSL9VKAejJLUnP/AwDckxwX eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGBgYAQAAAYAAg== 1.0 eAFzyk+p/P+PAQIBLnAGhg== colorset@rainbow Body 1 colorset@rainbow Body Body 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFjYGBgYGQEAAAJAAM= 1.0 Stencils Render 10.0 eAELzs/JTPnvlJ9S+f8fIwgWgwUAh60Lnw== 1 1 CellRegion CellRegion Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectCellRegion.Outputs.WholeCells.dataitem 3.0 0 CellRegionSelectCellRegion 1 Nuclei Select population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAENyzEOgDAIAMAf8Ymqq26diZBIQktTwMTXq7ffeqMmhlhfMBCOaSdTTnbYpFN5 woaiN9gzRoZDvUy5sKoD/UGC2/sBj7cbEA== eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xLca4syS/ISSzO/Q8AKaUGPQ== eAFjBQAABgAG 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 SelectCellRegion 0 1 UI.InputParameters.SelectCellRegion.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc38DwALhgNg Nucleus Select nucleus region, i.e. center region 0 Complex s CenterRegion Center Region Discrete 1 SelectCellRegion 1 1 eAHzK03OSS0t/g8ADvED3w== eAELSk3PzM/7DwALcgNk eAHzK03OSS0t/g8ADvED3w== eAHzK03OSS0t/g8ADvED3w== eAFzy8xL8StNzknN/A8AGaQE4Q== eAEz+Q8AAWkBNA== eAHzK03OSS0t/g8ADvED3w== eAHzK03OSS0t/g8ADvED3w== eAH7DwABAAEA eAHzK03OSS0t/g8ADvED3w== eAH7DwABAAEA eAHzyE9NziguUfBNLM7+DwAifQV6 eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjYGBgAAAABAAB 1.0 5.0 UI.InputParameters.SelectCellRegion.CenterRegion 0 eAHzK03OSS0t/g8ADvED3w== Nucleus eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJx7n8A868PPw== Cell Select cell region 0 Complex s RestrictiveRegion Restrictive Region Discrete 0 SelectCellRegion 1 0 UI.InputParameters.SelectCellRegion.RestrictiveRegion 0 eAFzTs3J+Q8ABgMCgA== Cell eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJx7n+/fCAXAEZOEc4= Resize Region [%] Select a method 0 Complex s Method Method Discrete % 0 SelectCellRegion 0 1 UI.InputParameters.SelectCellRegion.Method.title 1 eAELSi3OrEpVCEpNz8zPU4hWjf0PAD9fBvM= Resize Region [%] eAELSi3OrEpVCEpNz8zPU4hWjf0fhCpyaGuufkFF7H8ASvoQuw== Cytoplasm Region Select region type. Supported types are: Cell Region, Nucleus Region, Cytoplasm Region, Membarne Region and Ring Region. Please note also that region shape depends on unit type: relative unit options might result qualitively different regions compared to absolute unit (pixel/µm) ones. 0 eAFzTs3JUQhKTc/Mz/vvV5qck1paDOM6V5bkF+QkFufCBHxTc5OKEvNSYfygzLx0 GBsAPlMeYg== Complex s RegionType Region Type Discrete Cytoplasm Region Cytoplasm Region 1 SelectCellRegion 0 1 UI.InputParameters.SelectCellRegion.Method.RegionType 1 eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== Cytoplasm Region eAFzTs3JUQhKTc/Mz/vvV5qck1paDOM6V5bkF+QkFufCBHxTc5OKEvNSYfygzLx0 GBsAPlMeYg== % Unit 0 Complex s UnitType UnitType Numeric 1 0 SelectCellRegion 1 0 UI.InputParameters.SelectCellRegion.Method.UnitType 0 0 % 5 Outer border shift 0 Complex d OuterBorderShift Outer Border 100 -200 Numeric -40 1 SelectCellRegion 0 0 1 UI.InputParameters.SelectCellRegion.Method.OuterBorderShift 1 0 % -40 eAE7d+PRm28MXCJyGkY2bgFRKQBGEAaR 1.0 5 % Unit 0 Complex s UnitType2 UnitType Discrete 0 SelectCellRegion 1 1 UI.InputParameters.SelectCellRegion.Method.UnitType2 0 eAFT/Q8AAUsBJQ== % eAFT/Q8AAUsBJQ== 45 Inner border shift 0 Complex d InnerBorderShift Inner Border 100 -200 Numeric 100 1 SelectCellRegion 0 0 1 UI.InputParameters.SelectCellRegion.Method.InnerBorderShift 1 0 % 100 eAFjYAABlwNgisEESkN4DAwmDhCWC5T2g9IhUDrSAQCV1gSs 1.0 5 Cytoplasm Region Name of the created region 0 Complex s RegionName Output Region Numeric 0 SelectCellRegion 0 1 UI.InputParameters.SelectCellRegion.RegionName 1 0 Cytoplasm Region 124082 122842 3 1 3 5.0.1.124082 0 Output Region Cytoplasm Region eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== 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eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA 1 Method MethodFolder InputParameters.SelectCellRegion.Method Method RestrictiveRegion Method 1 1 0 1 builtin Each CalculateIntensityProperties_3 1 Replace me 1 1 Nuclei PopulationCalculateIntensityProperties_3 Population: Nuclei 0 sticky eAHz8Hd19ggOUTA2NjYx+g8AHFUELQ== eAHzK03OSS0t/g8ADvED3w== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationCalculateIntensityProperties_3 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQBAAAbAAc= 1.0 eAFzL0qs/P+PAQYBPcYHiQ== eAFzL0qs/P+PAQYBPcYHiQ== eAFjYGBgZGAAAAAJAAI= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYAABAAAGAAE= 1.0 eAEBMADP/3ZxJp974NA/X5hOH1ujyz/X1fQ5YjPPP4B/PIkGS9E/YK1knGGW0D+Q +5FRaKDIPz1hF7M= 1.0 eAHrKZ/2P6V/goPhRLkVc3w2OizpcFZ/29/joGmhW9f7ZY7DD1b2M5v/bHBgAIEd kxwAKmMU/Q== 1.0 eAEzb5/BtqEz0mHq5ugfV29EOTzd9fjoNIZIh6JaHVkN8xiHmgX99oenFDswQAEA JaASrg== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8IAA72BPw= eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjZAACAAAKAAI= 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFjYAACAAAFAAE= 1.0 eAFjYAACAAAFAAE= 1.0 eAFzyi9KSS36/48BAgE6EwdW eAFzL0qs/P+PAQYBPcYHiQ== 1 eAENzDEKgDAMBdAbZfEI6uCkm6ME/WAhtqX5ETy9Oj94460WylTyoFRZWtlxRINL r7aHKTFlInvi82NFY4JvnczBGnRZz2LoYeZy/EMirvcDmIoh5w== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAFzyi9KSS36/48BAgE6EwdW colorset@rainbow Border 1 colorset@rainbow Border Border 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFjZAACRgAADQAD 1.0 Stencils Render 10.0 eAFzyi9KSS36/48BAovzczJT/gMAb6YKcA== 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.CalculateIntensityProperties_3.Outputs.WholeCells.dataitem 3.0 0 PopulationCalculateIntensityProperties_3 1 Alexa 647 Signal channel 0 Complex s 1 1 channel_string Channel Discrete 1 CalculateIntensityProperties_3 0 1 UI.InputParameters.CalculateIntensityProperties_3.channel_string 1 eAHz8Hd19ggOUTA2NjYx+g8AHFUELQ== HOECHST 33342 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== Nuclei Select population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1gtOzUlNLnFOzckJSk0Hyuv5l5YU lJYU64Vn5OekgsSL9VKAejJLUnP/AwDckxwX eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAELTs1JTS5xTs3JCUpNz8zP+w8AO58HRA== eAFjAwAABwAH 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAFzy8xLca4syS/ISSzO/Q8AKaUGPQ== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 CalculateIntensityProperties_3 0 1 UI.InputParameters.CalculateIntensityProperties_3.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc38DwALhgNg Cell Select region which should be characterized. 0 Complex s Region Region Discrete 1 CalculateIntensityProperties_3 0 1 eAHzK03OSS0t/g8ADvED3w== eAELSk3PzM/7DwALcgNk eAHzK03OSS0t/g8ADvED3w== eAHzK03OSS0t/g8ADvED3w== eAFzy8xL8StNzknN/A8AGaQE4Q== eAEz+Q8AAWkBNA== eAHzK03OSS0t/g8ADvED3w== eAHzK03OSS0t/g8ADvED3w== eAH7DwABAAEA eAHzK03OSS0t/g8ADvED3w== eAH7DwABAAEA eAHzyE9NziguUfBNLM7+DwAifQV6 eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjYGBgAAAABAAB 1.0 5.0 UI.InputParameters.CalculateIntensityProperties_3.Region 1 eAHzK03OSS0t/g8ADvED3w== Nucleus eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== Standard Select a method 0 Complex s Method Method Discrete 0 0 0 1 0 0 50 0 0 0 CalculateIntensityProperties_3 0 1 UI.InputParameters.CalculateIntensityProperties_3.Method.title 1 eAELLknMS0ksSvkPABJkBDE= Standard eAELLknMS0ksSvkPABJkBDE= 1 Turn the Mean Intensity calculation ON/OFF 0 Complex b Mean_ONOFF Mean 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.Mean_ONOFF 1 0 1 1 0 Turn the Standard Deviation (StdDev) calculation ON/OFF 0 Complex b Stddev_ONOFF Standard Deviation 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.Stddev_ONOFF 1 0 0 1 0 Turn the Coefficient of Variance (CV) calculation ON/OFF 0 Complex b CV_ONOFF Coefficient of Variance 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.CV_ONOFF 1 0 0 1 0 Turn the Meadian Intensity calculation ON/OFF 0 Complex b Median_ONOFF Median 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.More.Median_ONOFF 1 0 0 1 0 Turn the summarized/integral intensity calculation ON/OFF 0 Complex b Sum_ONOFF Sum 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.More.Sum_ONOFF 1 0 0 1 0 Turn the Maximum Intensity calculation ON/OFF 0 Complex b Max_ONOFF Maximum 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.More.Max_ONOFF 1 0 0 1 0 Turn the Minimum Intensity calculation ON/OFF 0 Complex b Min_ONOFF Minimum 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.More.Min_ONOFF 1 0 0 1 50 For the quantile calculation all pixels are sorted according to their intensity. The quantile is the intensity of that pixel, which is brighter than the QuantileFraction number of pixels. The statistic "median" is the shorthand for "quantile" at 0.5. 0 Complex d QuantileFraction Quantile Fraction 100 0 Numeric 0 CalculateIntensityProperties_3 0 1 5 UI.InputParameters.CalculateIntensityProperties_3.Method.More.QuantileFraction 1 1 % 50 10 0 Turn the Quantile Intensity calculation ON/OFF 0 Complex b Quantile_ONOFF Numeric 0 CalculateIntensityProperties_3 0 1 UI.InputParameters.CalculateIntensityProperties_3.Method.More.QuantileFraction.qualifier 1 0 0 0 Turn the Contrast calculation ON/OFF 0 Complex b Contrast_ONOFF Contrast 1 0 Numeric 0 CalculateIntensityProperties_3 0 1 1 UI.InputParameters.CalculateIntensityProperties_3.Method.More.Contrast_ONOFF 1 0 0 1 Intensity Nucleus HOECHST 33342 Prefix for property names. 0 Complex s SuffixForPropertyNames Output Properties Numeric 1 Intensity Nucleus HOECHST 33342 Intensity Nucleus HOECHST 33342 0 0 CalculateIntensityProperties_3 0 1 UI.InputParameters.CalculateIntensityProperties_3.SuffixForPropertyNames 1 0 Intensity Nucleus HOECHST 33342 124082 124082 3 1 3 5.0.1.124082 0 Output Property Intensity Nucleus HOECHST 33342 Mean eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= 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InputParameters.CalculateIntensityProperties_3.Method.More CV_ONOFF 0 0 0 1 builtin Each CalculateIntensityProperties 1 Replace me 1 1 Nuclei PopulationCalculateIntensityProperties Population: Nuclei 0 sticky eAFLKQ5KTfkPAAjbAvI= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationCalculateIntensityProperties eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 MaxValue:Plain eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 MaxValue eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Plain eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZGBkYGQEAAATAAU= 1.0 eAFzL0qs/K/sZmloZGD2/x+DU05pKpD6xwAAeucKfg== eAFzL0qs/P+PAQYBPcYHiQ== eAFjYGRgZGAAAAAOAAM= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYGRgZGAAAAAOAAM= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYACCC5McJvia3Uz53OAA4jJYTHao5rf78eD3AghfbiuEdlnsAABZug1m 1.0 eAEzb5/BtqEz0uEwm2W8yeEoh6e7Hh+dxhDpcGxH3st4jxiHmgX99oenFDswQAEA Bu0Sow== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8IAA72BPw= eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGBgYAQAAAYAAg== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFjYAACAAAFAAE= 1.0 eAFjYAACAAAFAAE= 1.0 eAFzyi9KSS36/48BAgE6EwdW eAFzL0qs/K/sZmloZGD2/x+DU05pKpD6xwAAeucKfg== 1 eAENyzEKgDAMBdAb5RLVwUk359AGDMS2ND+Cp9fOj7c+bMHQVhcG0zFalhJDnBJb DmPIViHVFe/ELgM6cQ/0gNN5NZMkZk5lfoXc3w9b9SFV eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAFzyi9KSS36/48BAgE6EwdW colorset@rainbow Border 1 colorset@rainbow Border Border 0 MaxValue:Plain Colormode Render MaxValue Images Plain Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFjYGBgYGQEAAAJAAM= 1.0 Stencils Render 10.0 eAFzyi9KSS36/48BAovzczJT/gMAb6YKcA== 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.CalculateIntensityProperties.Outputs.WholeCells.dataitem 3.0 0 PopulationCalculateIntensityProperties 1 Alexa 647 Signal channel 0 Complex s 1 1 channel_string Channel Discrete 1 CalculateIntensityProperties 0 1 UI.InputParameters.CalculateIntensityProperties.channel_string 1 eAFLKQ5KTfkPAAjbAvI= dsRed eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== Nuclei Select population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAENzDEKgDAMBdAbZfEI6uCkm6ME/WAhtqX5ETy9Oj94460WylTyoFRZWtlxRINL r7aHKTFlInvi82NFY4JvnczBGnRZz2LoYeZy/EMirvcDmIoh5w== eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf+DwDILA0U eAFjBwAACAAI 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAELTs1JTS5xTs3JCUpNz8zP+w8AO58HRA== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 CalculateIntensityProperties 0 1 UI.InputParameters.CalculateIntensityProperties.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc38DwALhgNg Cell Select region which should be characterized. 0 Complex s Region Region Discrete 1 CalculateIntensityProperties 0 1 eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAELSk3PzM/7DwALcgNk eAGLD0pNz8zPM/0PABGZA/g= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAELTs1JTS5xTs3JCUpNz8zP+w8AO58HRA== eAEz+w8AAW0BNg== eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAH7DwABAAEA eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjYWBgAAAAFAAF 1.0 5.0 UI.InputParameters.CalculateIntensityProperties.Region 1 eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== Cytoplasm Region eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== Standard Select a method 0 Complex s Method Method Discrete 0 0 0 1 0 0 50 1 0 0 CalculateIntensityProperties 0 1 UI.InputParameters.CalculateIntensityProperties.Method.title 1 eAELLknMS0ksSvkPABJkBDE= Standard eAELLknMS0ksSvkPABJkBDE= 1 Turn the Mean Intensity calculation ON/OFF 0 Complex b Mean_ONOFF Mean 1 0 Numeric 0 CalculateIntensityProperties 0 1 1 UI.InputParameters.CalculateIntensityProperties.Method.Mean_ONOFF 1 0 1 1 0 Turn the Standard Deviation (StdDev) calculation ON/OFF 0 Complex b Stddev_ONOFF Standard Deviation 1 0 Numeric 0 CalculateIntensityProperties 0 1 1 UI.InputParameters.CalculateIntensityProperties.Method.Stddev_ONOFF 1 0 1 1 0 Turn the Coefficient of Variance (CV) calculation ON/OFF 0 Complex b CV_ONOFF Coefficient of Variance 1 0 Numeric 0 CalculateIntensityProperties 0 1 1 UI.InputParameters.CalculateIntensityProperties.Method.CV_ONOFF 1 0 0 1 0 Turn the Meadian Intensity calculation ON/OFF 0 Complex b Median_ONOFF Median 1 0 Numeric 0 CalculateIntensityProperties 0 1 1 UI.InputParameters.CalculateIntensityProperties.Method.More.Median_ONOFF 1 0 0 1 0 Turn the summarized/integral intensity calculation ON/OFF 0 Complex b Sum_ONOFF Sum 1 0 Numeric 0 CalculateIntensityProperties 0 1 1 UI.InputParameters.CalculateIntensityProperties.Method.More.Sum_ONOFF 1 0 0 1 0 Turn the Maximum Intensity calculation ON/OFF 0 Complex b Max_ONOFF Maximum 1 0 Numeric 0 CalculateIntensityProperties 0 1 1 UI.InputParameters.CalculateIntensityProperties.Method.More.Max_ONOFF 1 0 0 1 0 Turn the Minimum Intensity calculation ON/OFF 0 Complex b Min_ONOFF Minimum 1 0 Numeric 0 CalculateIntensityProperties 0 1 1 UI.InputParameters.CalculateIntensityProperties.Method.More.Min_ONOFF 1 0 0 1 50 For the quantile calculation all pixels are sorted according to their intensity. The quantile is the intensity of that pixel, which is brighter than the QuantileFraction number of pixels. The statistic "median" is the shorthand for "quantile" at 0.5. 0 Complex d QuantileFraction Quantile Fraction 100 0 Numeric 0 CalculateIntensityProperties 0 1 5 UI.InputParameters.CalculateIntensityProperties.Method.More.QuantileFraction 1 1 % 50 10 0 Turn the Quantile Intensity calculation ON/OFF 0 Complex b Quantile_ONOFF Numeric 0 CalculateIntensityProperties 0 1 UI.InputParameters.CalculateIntensityProperties.Method.More.QuantileFraction.qualifier 1 0 0 0 Turn the Contrast calculation ON/OFF 0 Complex b Contrast_ONOFF Contrast 1 0 Numeric 0 CalculateIntensityProperties 0 1 1 UI.InputParameters.CalculateIntensityProperties.Method.More.Contrast_ONOFF 1 0 0 1 Intensity Cytoplasm Region dsRed Prefix for property names. 0 Complex s SuffixForPropertyNames Output Properties Numeric 1 Intensity Cytoplasm Region dsRed Intensity Cytoplasm Region dsRed 0 0 CalculateIntensityProperties 0 1 UI.InputParameters.CalculateIntensityProperties.SuffixForPropertyNames 1 0 Intensity Cytoplasm Region TMRM 124082 122842 3 1 3 5.0.1.124082 0 Output Properties Intensity Cytoplasm Region TMRM Mean, Intensity Cytoplasm Region TMRM StdDev eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAF9j0EKwzAMBL/iB+TULwQCgcZ9glBdEdQqdonklLzeScHt0aeF3WFgATzNaLzR baNVcCftUUIWNBoXnAkuBcBjbM5tRVNQfA5CXMbIxihuQn11rpbX9HEhRVtRzaX7 k4Lpf6zMwPHR75begroAi2Q9+e+jn7qCNQ4CImWX eAFtjLEKgDAMRPXbOglaR0eJJWg0tGBS/fvWblZwe3e8O4srKF1IzFH0LBx8tmA/ OTWptdExUu48KQEPIEfuw22CLyPRcdnRqeRpC4wGmWuc37ML/5W6fQDeM0G5 eAFVizEOgCAMANWX8BcmEsXRGUmj1aYkUvT3VBzNLXfDediC4A2OqGS5midWH/jX tau9L5EA1TEKBjJTyKeO6TE2cduymHk9IErWOix7IrBA9MXHC8gZLK4= eAG1kksOwjAMRG+UBRyhgIQEBcGCZWXaUTG4SdU4RZweAkh0g1D5LbOY5xd7xi1J IGVnR6Rklo3LUYQG3iQkeRBSTCsqkQ3MImgd1JsUZQy0YJHgtbmFTRHTrKjO4w+A lH7H+rFah3tfbMK2SEMu4I5yf/bY0JPw1LIyyZz8oc+OnxBm7pg4G+/kdbHdI48X 7KmSnNTVQr56fGWzc4IEIj9hZN3VW/SRWkOi/3X+CmWs7IdeXbOtwnrWU6x9jUYZ Phv+AfoSeQERx2W2 eAFjYIADRgAADQAC 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5hAECTgXFmS X5CTWJwLFQMAh2Igiw== eAF9jEsKg0AQBY3eSI8wILgRSQ4gw/iQhp4PTrvw8t2SVcjGZVVBOc/hZC+Yot+x 9qaN+1Omr5HSNp+BQaattl90l+TCvkbT7gNGEAfmN3bKyX6DJEiV5FqOXHAIoa7D Y7Ybjno+Yw== eAFjYmJmZmFhYWVlY+cAAAEcADY= 1.0 eAFjYEAAAAAMAAE= 1.0 eAFjYmZiZmRmYWRiZGQEAAC8ABk= 1.0 eAFjYGBgYGVgYGNg52AAAAB3ABs= 1.0 eAFjZAQDBkYGBgYAAE8ACQ== 1.0 eAFVzDsKgDAQRVE/O9IlBAQbEV2AhPiQgTEJZlK4+YxYidzuFNdYdpmtYDztga3T UpkfaakH8vuUHYO0NG8vmFtCZJtOLe0KhhMD5gUHBa/fwgt8IrnnK0RcQkhbrw+m nDLi eAH7////f7/8vFQg9Y8BiEAQAJz+DYE= eAH7/x8BAE2+C/U= eAFjYEAAAAAMAAE= 1.0 eAH7////f4/81OSM4hIF38TibCD3HwMQgSAA+Q8Qaw== eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA 1 Method MethodFolder InputParameters.CalculateIntensityProperties.Method Method Region Method 0 0 0 MoreFolder InputParameters.CalculateIntensityProperties.Method.More CV_ONOFF 0 0 0 1 builtin Each CalculateProperties_5 1 Replace me 1 1 Nuclei PopulationCalculateProperties_5 Population: Nuclei 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzTs3J+Q8ABgMCgA== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationCalculateProperties_5 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQBAAAbAAc= 1.0 eAFzL0qs/P+PAQYBPcYHiQ== eAFzL0qs/P+PAQYBPcYHiQ== eAFjZAABAAAMAAI= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYAABAAAGAAE= 1.0 eAEBMADP//Idjm6p1Ms/LjHS6O/czD8nrMk6xD7MPxA/bCupiNM/0t7dRviZ0D+q 72IQad7IP4XyGlo= 1.0 eAE7qfXUxZFjhcP+L/ekzJ5scoja9j5ym/8EB0EBed8T2tMc3v5K2+Q1aZ0DAwgs 6HcAACu+FMA= 1.0 eAE7sywq+Xp5pEPeBSePyqIYh8RZLEAU6fDuH59e9eFoB8vACTdDpLMdGKAAAPjY EQs= 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8IAA72BPw= eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFjYAACAAAFAAE= 1.0 eAFjYAACAAAFAAE= 1.0 eAFzyi9KSS36n5SfUvn/HwMIAgBTDgkF eAFzL0qs/P+PAQYBPcYHiQ== 1 eAENyzEKgDAMBdAbZfME6qybo4T2g4VoS/Pj9bXrg7e+aqEs9VmUKnuvCTk6XGa1 FKbEsIbOAj8n2YIt6HJc1TDDzCWPWIj7+wFxXh4i eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAFzyi9KSS36n5SfUvn/HwMIAgBTDgkF colorset@rainbow body 1 colorset@rainbow Border Border 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFjYGRgYGAEAAAMAAM= 1.0 Stencils Render 10.0 eAFzyi9KSS36n5SfUvn/HwMIFufnZKb8BwCSuwwf 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.CalculateProperties_5.Outputs.WholeCells.dataitem 3.0 0 PopulationCalculateProperties_5 1 Nuclei Select a population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAENyzEKgDAMBdAb5RLVwUk359AGDMS2ND+Cp9fOj7c+bMHQVhcG0zFalhJDnBJb DmPIViHVFe/ELgM6cQ/0gNN5NZMkZk5lfoXc3w9b9SFV eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwt/g8Ar6MMgg== eAHjAAAACQAJ 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf+DwDILA0U eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 CalculateProperties_5 0 1 UI.InputParameters.CalculateProperties_5.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc38DwALhgNg By Formula Select a method 0 Complex s Method Method Discrete TMRM SD/Mean TMRM SD/Mean 0 CalculateProperties_5 0 1 UI.InputParameters.CalculateProperties_5.Method.title 1 eAFzqlRwyy/KLc1J/A8AF5oEsQ== By Formula eAFzqlRwyy/KLc1J/O9UqRCUmpNYkpqiEJBfABQpyczP+w8A2eMNlw== A/B Define a new property by formula, in style 100*(A-B)/C and thereupon specify below the corresponding variables A, B etc. Please use only single characters (A, B, .. X, Y). 0 set(FoundLetters=EvaluationData.Procedures[ProcedureIDName].Inputs.Formula.private.FoundLetters) Complex s Formula Formula Numeric eAFz/O/0HwAFiAKC 0 CalculateProperties_5 0 1 UI.InputParameters.CalculateProperties_5.Method.Formula 1 0 A/B Intensity Nucleus HOECHST 33342 Mean Select a property to which the variable A should correspond to 0 AAL::GetSelectedRegion(SelectedLabel=VariableA, RegionTable=ScalarTable| ScalarTable_A=TableForSelectedRegion, SelectedScalarRowIndex_A=SelectedRegionRowIndex) Complex s VariableA Variable A Discrete 0 CalculateProperties_5 0 1 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1MI8Q3yVQguSXFJLfsPACjPDvE= eAELTk7MSSz6DwALOQNW eAGLD05OzEksMv4PABFOA+g= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwt/g8Ar6MMgg== eAGz+A8AAXEBOA== eAH7DwABAAEA eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1MI8Q3yVQguSXFJLfsPACjPDvE= eAELLklxSS1TyMwrSc0rziypVMhPU3CuLMkvyEkszlUISk3PzM9TSKpUSM5IzMtL zVFIKQ5KTfkPACvvFKw= eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAELLklxSS1T8MwrSc0rziyp/A8AOVAHMQ== eAFLKQ5KTfkPAAjbAvI= eAEz+A8AAWEBMA== eAFzzi/NKynWD8isSM35DwAjwwWt eAELLklxSS1T8MwrSc0rziyp/A8AOVAHMQ== eAELLklxSS1TyMwrSc0rziypVMhPU3CuLMkvyEkszlUISk3PzM9TSKpUSM5IzMtL zVFIKQ5KTfkPACvvFKw= eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjYmBgAAAADAAD 1.0 5.0 UI.InputParameters.CalculateProperties_5.Method.VariableA 1 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1MI8Q3yVQguSXFJLfsPACjPDvE= Intensity Cytoplasm Region TMRM StdDev eAHzzCtJzSvOLKlU8CtNzkktLVbw8Hd19ggOUTA2NjYxUvBNTcz77wlX5FxZkl+Q k1icqxCUmp6Zn6cQ4hvkS5yi4JIUl9Sy/wAbOSnL Intensity Cytoplasm Region TMRM Mean Select a property to which the variable B should correspond to 0 AAL::GetSelectedRegion(SelectedLabel=VariableB, RegionTable=ScalarTable| ScalarTable_B=TableForSelectedRegion, SelectedScalarRowIndex_B=SelectedRegionRowIndex) Complex s VariableB Variable B Discrete 0 CalculateProperties_5 0 1 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1MI8Q3yVfBNTcz7DwAMdw4o eAELTk7MSSz6DwALOQNW eAGLD05OzEksMvoPABFMA+c= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwt/g8Ar6MMgg== eAGz+A8AAXEBOA== eAH7DwABAAEA eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1MI8Q3yVfBNTcz7DwAMdw4o eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R SCkOSk35DwAB/hPj eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAFLKQ5KTfkPAAjbAvI= eAEz+A8AAWEBMA== eAFzzi/NKynWD8isSM35DwAjwwWt eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R SCkOSk35DwAB/hPj eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjZGBgAAAACAAC 1.0 5.0 UI.InputParameters.CalculateProperties_5.Method.VariableB 1 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1MI8Q3yVfBNTcz7DwAMdw4o Intensity Cytoplasm Region TMRM Mean eAHzzCtJzSvOLKlU8CtNzkktLVbw8Hd19ggOUTA2NjYxUvBNTcz77wlX5FxZkl+Q k1icqxCUmp6Zn6cQ4hvkS5yi4JIUl9Sy/wAbOSnL Formula Name of the calculated property 0 Complex s PropertyName Output Property Numeric 0 CalculateProperties_5 0 1 UI.InputParameters.CalculateProperties_5.PropertyName 1 0 TMRM SD/Mean 124082 124082 5 1 5 5.0.1.124082 0 Output Property TMRM SD/Mean eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAF9j1EKwjAQRK+SA/TLKxSEgsYjLGu6lNVtUrqbSk8fFaLQn3wNzDweDICnCY03 um20Cu6kPUrIgkbDjBPBqQB4jM25rWgKis9BiMsQ2RjFXVGfnavlJb1cSNFWVHPp /qBg+h8rc+Y49rulRVBnYJGsH/776Keu4DHe1OBo9g== eAF1jLEKgDAMRPXbnASto6PEEjQaGrBp/fvWbiro9u54dwYXUIpIzMHrUVhcNmBe OVWpNsEyUm4dKQH34PfcydmIKyOvw7yhVZ/HVRgbZH7idJ9F/FZ+2gvLjkaq eAFVizEOgCAMANWX8BcmEsXRGUmj1aYkUvX3VIyTueVuOA9LELzAEZ1ZjuqJ1Qf+ dWlK689IgOoYBQOZIeRd+3Qbm7huWcw4bxAla+mmNRFYIHrj4wEjgi2u eAG1kkFugzAQRW/kRaOcgFIpUgtRuugSTeGLTjLYCI+pcvrUTaTSBaoI0KUX/83z zE97kkDKzj6Sktl3rkQVOniTkJRBSLFrqEbxYPKgbVBvMtQx0INFgtfuGjZVTLOi uaQzgJQtY62sNuDuF3tiW2WhFPBAuT0nbGgkvLOsTPJC/jRlxyOEZ/eZOBvv5DV/ P6KMF5yokpzVtUK++fnK24cTJBBZhVEMV+8xReoVEv2/5x9Qx8rO9BqabRXWs55j 7Vt0yvDF5h+gS5G/9LZ/sr4AtqOD1w== eAFjYEAARgAADgAC 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5hAECTgXFmS X5CTWJwLFfvHBADKdiGL eAF9jEEKgzAQRau9kaUnCBS6KaIHkBA/YWBMxIwLLz8jrqwbl+8//nOew8pe8J18 xNCYPtxlMq0+lMbfGhhkWmt9oNskz+zLZPrswQjiwNwhUk52BpIgFZKtXfKMRQhl eN3qU/5d3rYDUDVHsQ== eAFjYmJmZmFhYWVlY+fgBAABWwA/ 1.0 eAFjYEACAAANAAE= 1.0 eAFjYmZiZmRmYWRiBAIAANYAGg== 1.0 eAFjYGBgYGVgYGNg5+BkAAAApAAk 1.0 eAFjZAQDBkYGIAAAAFgACQ== 1.0 eAF9jDsKgDAQBf3cSI8QEGxE9AAS4iMsrImYtfDyWbESG5luBsZYdidbQb9Zj6XR XJiP0lx2FNbhdAzSXD08wlwSd7Zp01zPYDgxYJ7gKQZ9F0EQEsk1HnHHIYS0tL9Z bxolP2M= eAH7////f7/8vFQg9Y8BiCAQALn8Dn8= eAH7/x8JAABasgz0 eAFjYEACAAANAAE= 1.0 eAH7////f4/81OSM4hIF38TibCD3HwMQQSAAG/ARaQ== eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA 1 Method MethodFolder InputParameters.CalculateProperties_5.Method Method WholeCells_string Method 1 1 0 1 builtin Each CalculateIntensityProperties_2 1 Replace me 1 1 Nuclei PopulationCalculateIntensityProperties_2 Population: Nuclei 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationCalculateIntensityProperties_2 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 MaxValue:Plain eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 MaxValue eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Plain eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZGAEAgAAFgAG 1.0 eAFTdnN2NTN0+6/sZmloZGD2370osfL/PyYQBAB+6Aqa eAFzL0qs/P+PAQYBPcYHiQ== eAFjZGQAAgAAEQAD 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjZGRgYGQAAAATAAQ= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYACCC5McJGJLLrpub3YAcRksJkPoPUshtNxWCO2y2AEACOwKAg== 1.0 eAEzb5/BtqEz0uHtVtnf0c+iHJ7uenx0GkOkQ1GtjqyGeYxDzYJ++8NTih0YoAAA G6sSYQ== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8IAA72BPw= eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGBgYAQAAAYAAg== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFjYAACAAAFAAE= 1.0 eAFjYAACAAAFAAE= 1.0 eAFzyi9KSS36/48BAgE6EwdW eAFTdnN2NTN0+6/sZmloZGD2370osfL/P6Z/TABptgma 1 eAENzDEKgDAMBdAbZfAK6uCkm6ME/WAhtqX5ETy9Oj94460WylTyoFRZWtlxRINL r7aHKTFlInvi82NFY4JvnczBGnRZz2LoYeZy/EMirvcDmGwh5g== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAFzyi9KSS36/48BAgE6EwdW colorset@rainbow Border 1 colorset@rainbow Border Border 0 MaxValue:Plain Colormode Render MaxValue Images Plain Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQKV3dwsjQ0c/wMA9eQOIA== eAFjYGBgYGQEAAAJAAM= 1.0 Stencils Render 10.0 eAFzyi9KSS36/48BAovzczJT/gMAb6YKcA== 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.CalculateIntensityProperties_2.Outputs.WholeCells.dataitem 3.0 0 PopulationCalculateIntensityProperties_2 1 Alexa 647 Signal channel 0 Complex s 1 1 channel_string Channel Discrete 1 CalculateIntensityProperties_2 0 1 UI.InputParameters.CalculateIntensityProperties_2.channel_string 1 eAFzzEmtSFQwMzH/DwASlwOs Alexa 647 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== Nuclei Select population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAENyzEKgDAMBdAbZfME6qybo4T2g4VoS/Pj9bXrg7e+aqEs9VmUKnuvCTk6XGa1 FKbEsIbOAj8n2YIt6HJc1TDDzCWPWIj7+wFxXh4i eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzTsxJLs1JLEkNKMovSC0qyUwtjjf9DwBk9QlP eAHjBAAACgAK 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwt/g8Ar6MMgg== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 CalculateIntensityProperties_2 0 1 UI.InputParameters.CalculateIntensityProperties_2.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc38DwALhgNg Cell Select region which should be characterized. 0 Complex s Region Region Discrete 1 CalculateIntensityProperties_2 0 1 eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAELSk3PzM/7DwALcgNk eAGLD0pNz8zPM/0PABGZA/g= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAELTs1JTS5xTs3JCUpNz8zP+w8AO58HRA== eAEz+w8AAW0BNg== eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAH7DwABAAEA eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjYWBgAAAAFAAF 1.0 5.0 UI.InputParameters.CalculateIntensityProperties_2.Region 1 eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== Cytoplasm Region eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== Standard Select a method 0 Complex s Method Method Discrete 0 0 0 1 0 0 50 0 0 0 CalculateIntensityProperties_2 0 1 UI.InputParameters.CalculateIntensityProperties_2.Method.title 1 eAELLknMS0ksSvkPABJkBDE= Standard eAELLknMS0ksSvkPABJkBDE= 1 Turn the Mean Intensity calculation ON/OFF 0 Complex b Mean_ONOFF Mean 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.Mean_ONOFF 1 0 1 1 0 Turn the Standard Deviation (StdDev) calculation ON/OFF 0 Complex b Stddev_ONOFF Standard Deviation 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.Stddev_ONOFF 1 0 0 1 0 Turn the Coefficient of Variance (CV) calculation ON/OFF 0 Complex b CV_ONOFF Coefficient of Variance 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.CV_ONOFF 1 0 0 1 0 Turn the Meadian Intensity calculation ON/OFF 0 Complex b Median_ONOFF Median 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.More.Median_ONOFF 1 0 0 1 0 Turn the summarized/integral intensity calculation ON/OFF 0 Complex b Sum_ONOFF Sum 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.More.Sum_ONOFF 1 0 0 1 0 Turn the Maximum Intensity calculation ON/OFF 0 Complex b Max_ONOFF Maximum 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.More.Max_ONOFF 1 0 0 1 0 Turn the Minimum Intensity calculation ON/OFF 0 Complex b Min_ONOFF Minimum 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.More.Min_ONOFF 1 0 0 1 50 For the quantile calculation all pixels are sorted according to their intensity. The quantile is the intensity of that pixel, which is brighter than the QuantileFraction number of pixels. The statistic "median" is the shorthand for "quantile" at 0.5. 0 Complex d QuantileFraction Quantile Fraction 100 0 Numeric 0 CalculateIntensityProperties_2 0 1 5 UI.InputParameters.CalculateIntensityProperties_2.Method.More.QuantileFraction 1 1 % 50 10 0 Turn the Quantile Intensity calculation ON/OFF 0 Complex b Quantile_ONOFF Numeric 0 CalculateIntensityProperties_2 0 1 UI.InputParameters.CalculateIntensityProperties_2.Method.More.QuantileFraction.qualifier 1 0 0 0 Turn the Contrast calculation ON/OFF 0 Complex b Contrast_ONOFF Contrast 1 0 Numeric 0 CalculateIntensityProperties_2 0 1 1 UI.InputParameters.CalculateIntensityProperties_2.Method.More.Contrast_ONOFF 1 0 0 1 Intensity Cytoplasm Region Alexa 647 Prefix for property names. 0 Complex s SuffixForPropertyNames Output Properties Numeric 1 Intensity Cytoplasm Region Alexa 647 Intensity Cytoplasm Region Alexa 647 0 0 CalculateIntensityProperties_2 0 1 UI.InputParameters.CalculateIntensityProperties_2.SuffixForPropertyNames 1 0 Intensity Cytoplasm Region Alexa 647 124082 122842 3 1 3 5.0.1.124082 0 Output Property Intensity Cytoplasm Region Alexa 647 Mean eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAF9j1EKwjAQRK+SA/TLKxSEgsYjLGu6lNVtUrqbSk8fFaJfJV8DM48HA+BpQuON bhutgjtpjxKyoNEw40RwKgAeY3NuK5qC4nMQ4jJENkZxV9Rn52p5SS8XUrQV1Vy6 PyiY/sfKnDmO/W5pEdQZWCTrh/8++qkreBhvv0ZsVQ== eAHzS01PLMksS83MySktLikCsvPz/vsl+qHw/zH8Y/QrTc5JzfzvmZdZkpmY45tY nP3fJ7/cOT8PqKm4xD8pKzW5pPh/eEZ+Tqpzak4OMjMeYVhZKnYlhEUB70RLmw== eAFVizEOgCAMANWX8BcmEsXRGUmj1aYkUvX3VBwczC13w3lYguAFjujMclRPrD7w r0tTWn9GAlTHKBjIDCHv2qfb2MR1y2LGeYMoWUs3rYnAAtEbHw+A3C6u eAG1k0FuwjAQRW/kBVVPkIIUCUJVFiyjafIVpp3YUTxOxemLAQmzyCIldOnFf37j P14OJIGUnX0jJfPeuwp16OFNRlIFIUXeUoNyYbZBu6DeFGhiYACLBK/9JWzqmGZF +7t8AEjFPNaT1RLu72IrtnURKgEnyvU44YVGwrllZZIN+e8pbzxCWLufzNnYk9ft 5xeq2OBEleyorhPy7W2U/cEJMog8hVGm1gdMkdpBov/5/g80cWUf9EqbbRXWsx7j 2nfoleHLl3+AzkXe6b3OZeUjM6e/PVbwCRfApbw= eAFjYEACjAAADwAC 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5hAECTgXFmS X5CTWJwLFQOJAwAPmSKL eAF9jEEKgzAQRVt7I0tPEBDcFGkPICF+ZGBMxIwLLz8jrmJBuvzv8Z/zHFb2gnby I/ra9OZ+kOm9oTi818Ag00qrY7pN0sw+T6aPLxhBHJg/GClFK4EoiJlk65Y0YxFC 7p9/dZGny6vQ9qJY2w7HNlTD eAFjYmJmZmFhYWVlY+fg5AIAAaQASQ== 1.0 eAFjYEAGAAAOAAE= 1.0 eAFjYmZiZmRmYWRiBAEAAPEAGw== 1.0 eAFjYGBgYGVgYGNg5+DkYgAAANwALg== 1.0 eAFjZAQDBkYGEAAAAGEACQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5hAECTgXFmS X5CTWJz7/x9zcGpOanKJc2pOTlBqemZ+3n+EEXklqXnFmSWVAUX5BalFJZmpxfHG eKURkkhaTP8DAPHRSLE= eAH7////f7/8vFQg9Y8BiGAQANj2D30= eAH7/x8ZAABopQ3z eAFjYEAGAAAOAAE= 1.0 eAH7////f4/81OSM4hIF38TibCD3HwMQwSAAQL4SZw== eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA 1 Method MethodFolder InputParameters.CalculateIntensityProperties_2.Method Method Region Method 0 0 0 MoreFolder InputParameters.CalculateIntensityProperties_2.Method.More CV_ONOFF 0 0 0 1 builtin Each SelectPopulation_4 1 Replace me 1 1 Nuclei PopulationSelectPopulation_4 Population: Nuclei 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAELTs1JTS5JTVHwT8oCMor/u2QWJycWpSCJAAD5nw5/ Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationSelectPopulation_4 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 MaxValue:Plain eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 MaxValue eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Plain eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZGAEAgAAFgAG 1.0 eAFzL0qs/K/sZmloZGD2/x8DBAIAXqwI9Q== eAFzL0qs/P+PAQYBPcYHiQ== eAFjZGQAAgAAEQAD 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYGQAAgAACwAC 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYACCBV0OHXq7XizavdQBxGVgiHMAUxvqIPTlTRD6Qb0DACQHC3U= 1.0 eAHjtZ/7cPnkSIe8C04elUUxDtsmHpWPvRrhcGGx4aoJITEOPXq7Xih1FzswQAEA DWgSbA== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8MABvrBvo= eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFjYAACRkYAAAoAAw== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9z84NSc1 uSQ1RcE/KQvIKP7vklmcnFiUgiQCAEB4JPw= eAFjYAABRgAACAAC 1.0 eAFjYAADAAAHAAE= 1.0 eAFzyk+p/P+PAQKd8otSUov+/2MEAH0hCuI= eAFzL0qs/K/sZmloZGD2/x8DBAIAXqwI9Q== 1 eAEdzDEKgDAMQNEbZfIIKjhZcHAsoQ1YiKY0idfX0vl9/vIiO1qRZ0ZDCE0SZW+k cBBTsiDVeXicYHerbgrnJd2IWeMqbWN2tTYiyP1SjO7vBxyuI6E= eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFjYAACRkYAAAoAAw== 1.0 eAFzyk+p/P+PAQKTwBxGAGo0CjI= green body 1 colorset@rainbow Body colorset@rainbow Body Body 0 MaxValue:Plain Colormode Render MaxValue Images Plain Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8V3ZzszQ2cPwPAK5VE2o= eAFjYAACRkZGAAAOAAQ= 1.0 Stencils Render 10.0 eAFzyk+p/P+PAQKd8otSUov+/2Mszs/JTPkPAMf8Dfw= 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation_4.Outputs.Wholecells_ForIllustration.dataitem 3.0 1 AnnexinV_neg cells FilteredSelectPopulation_4 Population: AnnexinV_neg cells 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzTs3J+Q8ABgMCgA== Standard 0 eAELKMovSC0qyUwtVnDMy0utyMwLi89LTVdITs3JKf4PALZnDEA= FeedBack.FilteredSelectPopulation_4 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQBAAAbAAc= 1.0 eAFzL0qs/P+PAQYBPcYHiQ== eAFzL0qs/P+PAQYBPcYHiQ== eAFjZAABAAAMAAI= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYAABAAAGAAE= 1.0 eAEBMADP/9QQmgn6wtU/CBhPMeXgyT8YGP5f7YXhP7gsJsyntdc/D2CFG2P6zj8+ YNPo6hTIP0EoGAM= 1.0 eAGz3TCDXUy6zmHOuSNS8xs2O7xrUTmzlCnRoe30zle5T5sdJGJLLvpmbnZgAIEN 7Q4AEUEUYw== 1.0 eAHjtZ/7cPnkSIdfEWf8LG9EOWybeFQ+9mqEw4XFhqsmhMQ49OjteqHUXezAAAUA KwgTNA== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8IAA72BPw= eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFjYAACAAAFAAE= 1.0 eAFjYAACAAAFAAE= 1.0 eAFzyk+p/J8EIv4xgCAARPsINQ== eAFzL0qs/P+PAQYBPcYHiQ== 1 eAEdyzEKgDAMBdAbZfIG6mzBwbGE9ouFaEqbeH0V58ebbxZnK3pNbEyhaUL2hk4r BMmCVpff40CLW3XrtB0qGCHS417E0JApf70YzucFLOEgnw== 1 PopulationSelectPopulation_4 eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAFzyk+p/J8EIv4xgCAARPsINQ== colorset@rainbow body 1 colorset@rainbow Body Body 0 1 1 Filtered Filtered Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation_4.Outputs.WholeCells_filtered.dataitem 3.0 0 PopulationSelectPopulation_4 1 Nuclei Select a population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAENzDEKgDAMBdAbZfAK6uCkm6ME/WAhtqX5ETy9Oj94460WylTyoFRZWtlxRINL r7aHKTFlInvi82NFY4JvnczBGnRZz2LoYeZy/EMirvcDmGwh5g== eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf6DwDIKg0T eAHjAgAACwAL 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAFzTsxJLs1JLEkNKMovSC0qyUwtjjf9DwBk9QlP eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 SelectPopulation_4 0 1 UI.InputParameters.SelectPopulation_4.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc38DwALhgNg Filter by Property Select a method 0 Complex s Method Method Discrete 0 SelectPopulation_4 0 1 UI.InputParameters.SelectPopulation_4.Method.title 1 eAFzy8wpSS1SSKpUCCjKL0gtKqn8DwBG/Afm Filter by Property eAFzy8wpSS1SSKpUCCjKL0gtKqn875yfm5ufp+AGlin+75OZl5pYpOCck1hcnJmW mVr0Pzg1JzW5BKTHN7E4+z8Anq0bzA== 0.0 Define a proper value for the selection criterion 0 Complex d Attribute1 3.00000000000000018e+34 -3.00000000000000018e+34 Numeric 0 SelectPopulation_4 0 0 UI.InputParameters.SelectPopulation_4.Method.Attribute1 1 0 0 eAE7ewYIeowOMEDBWQjfAUI7ORiDwGVvKD/IAazMIBwqHu0wayYQWCZA5ZMg8g9S oOrSIXRDpgMADfgmtA== 1.0 18.5500000000000007 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName1, RegionTable=ScalarTable| ScalarTable_1=TableForSelectedRegion, SelectedScalarRowIndex_1=SelectedRegionRowIndex) Complex s AttributeName1 Discrete _Scalar5>0 and _Scalar5<140 3 3 eAGLdzeM/x/vbgQijOP/AwAxDQaj 0 SelectPopulation_4 0 0 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= eAELTk7MSSz6DwALOQNW eAGLD05OzEksMv0PABFSA+o= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf6DwDIKg0T eAEzNPgPAAH1AWE= eAH7DwABAAEA eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cMxJrUhUMDMx/w8AT34UnQ== eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAFzzEmtSFQwMzH/DwASlwOs eAEz+A8AAWEBMA== eAFzzi/NKynWD8isSM35DwAjwwWt eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cMxJrUhUMDMx/w8AT34UnQ== eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjYWBgAAAAFAAF 1.0 5.0 UI.InputParameters.SelectPopulation_4.Method.Attribute1.label 1 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= Intensity Cytoplasm Region Alexa 647 Mean eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP08hxDfIlzhFwSUpLqll/8Eagl30CRnsmJNakahgZmIOMR0A8SA/ Aw== > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier1 Discrete 0 SelectPopulation_4 0 0 UI.InputParameters.SelectPopulation_4.Method.Attribute1.qualifier 1 eAGz+w8AAX0BPg== > eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB 0.0 Define a proper value for the selection criterion 0 Complex d Attribute2 3.00000000000000018e+34 -3.00000000000000018e+34 Numeric 0 SelectPopulation_4 0 0 UI.InputParameters.SelectPopulation_4.Method.Attribute2 1 0 140 eAHjur64wLYrwiENBNqiHBhAoCEGSsc5nD0DBDYJDrNmAoFlIpROgqg3S4bSKQ7G YJAK0WeQBqXTIfp1MhwAch0jOg== 1.0 7.93700000000000028 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName2, RegionTable=ScalarTable| ScalarTable_2=TableForSelectedRegion, SelectedScalarRowIndex_2=SelectedRegionRowIndex) Complex s AttributeName2 Discrete 0 SelectPopulation_4 0 0 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= eAELTk7MSSz6DwALOQNW eAGLD05OzEksMv0PABFSA+o= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf6DwDIKg0T eAEzNPgPAAH1AWE= eAH7DwABAAEA eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cMxJrUhUMDMx/w8AT34UnQ== eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAFzzEmtSFQwMzH/DwASlwOs eAEz+A8AAWEBMA== eAFzzi/NKynWD8isSM35DwAjwwWt eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cMxJrUhUMDMx/w8AT34UnQ== eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjYWBgAAAAFAAF 1.0 5.0 UI.InputParameters.SelectPopulation_4.Method.Attribute2.label 1 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= Intensity Cytoplasm Region Alexa 647 Mean eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP08hxDfIlzhFwSUpLqll/8Eagl30CRnsmJNakahgZmIOMR0A8SA/ Aw== > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier2 Discrete 0 SelectPopulation_4 0 0 UI.InputParameters.SelectPopulation_4.Method.Attribute2.qualifier 1 eAGz+Q8AAXkBPA== < eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB 0.0 Define a proper value for the selection criterion 0 Complex d Attribute3 Numeric 0 SelectPopulation_4 0 0 UI.InputParameters.SelectPopulation_4.Method.Attribute3 1 0 0 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName3, RegionTable=ScalarTable| ScalarTable_3=TableForSelectedRegion, SelectedScalarRowIndex_3=SelectedRegionRowIndex) Complex s AttributeName3 Discrete 0 SelectPopulation_4 0 0 eAHzy89L/Q8ABloCkA== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA UI.InputParameters.SelectPopulation_4.Method.Attribute3.label 1 eAH7DwABAAEA eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP08hxDfIlzhFwSUpLqll/8Eagl30CRnsmJNakahgZmIOMR0A8SA/ Aw== > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier3 Discrete 0 SelectPopulation_4 0 0 UI.InputParameters.SelectPopulation_4.Method.Attribute3.qualifier 1 eAGz+w8AAX0BPg== > eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB F1 and F2 Defines boolean operations between filters, e.g. (F1 or F2) and F3. As default AND operations are applied. F1, F2 ... correspond to the first and to the second filter, respectively. 0 Complex s BooleanOperations Boolean Operations Numeric 1 F1 and F2 0 SelectPopulation_4 0 1 UI.InputParameters.SelectPopulation_4.Method.More.BooleanOperations 1 0 F1 and F2 Nuclei Selected Name of the output population. 0 Complex s OutputName Output Population Numeric 0 Nuclei Selected Nuclei Selected 1 0 SelectPopulation_4 0 1 UI.InputParameters.SelectPopulation_4.OutputName 1 0 AnnexinV_neg cells 124082 122842 4.0 1 eAFzzMtLrcjMC4vPS01XSE7NySn+DwBJzgfz 4.0 5.0.1.124082 0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAELSk3PzM/7/48BAgE6agdc eAHzK03OSS0t/h8flJqemZ9nBGMYwxgmMIbpfwC2LBO1 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFzy8xL8StNzknN/O8GZDpXluQX5CQW5/7/x/iPMTg1JzW5xDk1JycoNT0zP+8/ ANRdFF4= eAEz+W/6/x/jP0az/wAfCQWb eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAH775yak6OQVKmQnJGYl5eao+CcmJNcmpNYkpqi4JmbmJ6qo5CbWpKRn6Lg9B+i NL8oJbWIWB2VJfkFOYnFuUSq/w8AB+c1/g== eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmfZ636HwBf fxbe eAH7/x8IAA72BPw= eAHzyE9NziguUfBNLM7+75yYk1yak1iSmqLgmZuYnvr/H+M/xv8AAJwPaw== eAEz+P+PAQIBHrkFKA== eAH7/x8IAA72BPw= eAH7/x8IAA72BPw= eAH7/x8IAA72BPw= eAFL+f+PAQIBIMEFXA== eAH7/x8IAA72BPw= eAH7/x8IAA72BPw= 5.0 Output Population AnnexinV_neg cells eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAF9kEEKwkAMRa8yB3AlXkAKQkGrIOgyxDHU0TQpnbTa04+t1C67Csn7PJIAFFSi hY6OHTWMPcUM2beMRnmFJcE6ARQoi3hZsShIReuZQsolWEB2B4yvlZuGe307r2IN RnN6e5K3OMMpswtyz3rTmjFWEJjbOOTHi/7qKbhUtiL0CXIBodJ5Yo7D0teHMv0a 2GmTz2aVMw3ATlqPfwoqsElfFLaLIw== eAGdT8sKgzAQbL9NEAQbjz2GmI7t6pKFZLV/n2jxUAueepsZ5sEYPJ3SAmKek8YN SyjGmR+eL/lqZs+g0gRScnxzaSqtvCsJWyhp14/wmsr9JYwKzEdov2ULzi1/qnYg VkQ89rz/DNtaYnN8swLpJGTM eAFVjcEKgCAQRKsv8V+EICg7dgyTpazFhdzq77XsUjKXN8wMo2DWbE9oEA/P+8Pk otIu86EIpToMgo2Ns2w1ik77LbZ0CUnumXkW/bSCYR9DNSyEIAExmb++YHzRpM5Y 05693aKzQKA= eAG1lMtOwzAQRf/ICx5fEFopEqRVWbC0BmcIAxM7ssdB/XpwqIRZBMlNytKLOT72 vfZmBI4g5OwdCKi9dwbb6DGoCthEBsG6hw71ldpFGaIE1WCXBkYk5hjEfw+rNk2T YP+5WQCEZh3rwmoZd77YlmzbRMNImXJaFtzQzHBtSQj4AcJ7yR3PEO7dR+VsyinI 7vkNTUqwUKU6ihsYQv9zlKdXx1gh80UYOqc+YonUI3Lyn/Y/YJcqu9ArN9sK2kBy TLUf0Ath0Nf/AF2L/KV3u5ZVz5w5v+3zAj7lsXfD9E+kPPTNQrsikH4hFvTYllfl bzUzlVhvna9nf7EvJ0UG+Q== eAFjYEAGjIwMAAAWAAM= 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5hAECTgXFmS X5CTWJwLFYPIBKfmpCaXBOQXgMzMzM+LN/n/jwUAn+Isqg== eAGNj0EKwjAURLXeqOoJAkI3UvQAIcShBH6T0Pwuevn/a3ERBRGX84YZZowjP5Nj dKMbYFuVnflAKvtLiI/r7AlBpZFmk2bhlMmVUeVwB8GzAdENQ0hRa0FkxBJ46aeU MXFAscefdjXfIudKuy+Nrb4W9ClvT54L7OlPtAJblW0a eAFjYmJmZmFhYWVlY+fg5OLm5gYAAsEAag== 1.0 eAFjYEADAAARAAE= 1.0 eAFjYmZiZmRmYWRiBAMmZgABTAAh 1.0 eAFjYGBgYGVgYGNg5+Dk4gZyAAGSADk= 1.0 eAFjZAQDBkYGMGBkBAAAfwAL 1.0 eAF9j80KwjAQhP15o6pPEBB6kaIPEEIcysI2Cc320JfPavAQhSJz+4YZZoxjv7AT 9JMbYTstO/ODtOyvFJ63xTNIy6GqArNKTOzypOX4AMOLAfMdI8WgrSIIQiZZhzkm zELI9vTXbuZX5NJov9HY6WfBEFP98l5gz1voBXaaZf0= eAH7////f7/8vFQg9Y8BiJDhfwAvZRJ4 eAH7/x8NAACYeBDw eAFjYEADAAARAAE= 1.0 eAH7////f4/81OSM4hIF38TibCD3HwMQIeD//wCRTxVj eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA eAELKUrMzMvMS3dJLEn8DwAkdQW2 1 Method MethodFolder InputParameters.SelectPopulation_4.Method Method WholeCells_string Method 1 1 0 MoreFolder InputParameters.SelectPopulation_4.Method.More AttributeQualifier3 0 0 0 1 builtin Each SelectPopulation_5 1 Replace me 1 1 Nuclei PopulationSelectPopulation_5 Population: Nuclei 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAELTs1JTS5JTVHwT8oCMor/u2QWJycWpSCJAAD5nw5/ Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationSelectPopulation_5 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 MaxValue:Plain eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 MaxValue eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Plain eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 eAFjAAAAAQAB 1.0 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 eAFjAAAAAQAB 1.0 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 eAFjAAAAAQAB 1.0 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 eAFjAAAAAQAB 1.0 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZGAEAgAAFgAG 1.0 eAFTdnN2dTM1+O9elFj5/x+jU05pKpD6xwgAdnoKsg== eAFzL0qs/P+PAQYBPcYHiQ== eAFjZGRgZGAAAAAUAAQ= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjZGRgZGQAAAAWAAU= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYACCC5MczH88aOaXm+AA4jJYTIbQe5ZCaLmtENplsQMAD1oKGA== 1.0 eAEzb5/BtqEz0oEx6foi1alRDk93PT46jSHSoahWR1bDPMahZkG//eEpxQ4MUAAA 5isQ/A== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8MABvrBvo= eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFjYAACRkYAAAoAAw== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9z84NSc1 uSQ1RcE/KQvIKP7vklmcnFiUgiQCAEB4JPw= eAFjYAACRkYAAAoAAw== 1.0 eAFjYAADAAAHAAE= 1.0 eAFzyk+p/P+PAQKd8otSUov+/2MEAH0hCuI= eAFTdnN2dTM1+O9elFj5/x+jU05pKpD6xwgAdnoKsg== 1 eAEdzDEKgDAMQNEbZfMGKjhZcHAsoQ1YiKY0idfX0vl9/vIiO1qRZ0ZDCE0SZW+k cBBTsiDVeXicYHerbgrnJd2IWeMqbWN2tTYiyP1SjO7vBxzcI6I= eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8BwAevRDf eAFjYAACRkYAAAoAAw== 1.0 eAFzyk+p/P+PAQKTwBxGAGo0CjI= green body 1 colorset@rainbow Body colorset@rainbow Body Body 0 MaxValue:Plain Colormode Render MaxValue Images Plain Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQLTi1JT8/4Xpab8V3ZzszQ2cPwPAK5VE2o= eAFjYAACRkZGAAAOAAQ= 1.0 Stencils Render 10.0 eAFzyk+p/P+PAQKd8otSUov+/2Mszs/JTPkPAMf8Dfw= 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation_5.Outputs.Wholecells_ForIllustration.dataitem 3.0 1 AnnexinV_pos cell FilteredSelectPopulation_5 Population: AnnexinV_pos cell 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzTs3J+Q8ABgMCgA== Standard 0 eAELKMovSC0qyUwtVnDMy0utyMwLiy/IL1ZITs3J+Q8Aq2kL5Q== FeedBack.FilteredSelectPopulation_5 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 eAFjAAAAAQAB 1.0 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 eAFjAAAAAQAB 1.0 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 eAFjAAAAAQAB 1.0 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 eAFjAAAAAQAB 1.0 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQBAAAbAAc= 1.0 eAFzL0qs/P+PAQYBPcYHiQ== eAFzL0qs/P+PAQYBPcYHiQ== eAFjZAABAAAMAAI= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYAABAAAGAAE= 1.0 eAEBMADP/3ZxJp974NA/X5hOH1ujyz/X1fQ5YjPPP4B/PIkGS9E/YK1knGGW0D+Q +5FRaKDIPz1hF7M= 1.0 eAHrKZ/2P6V/goPhRLkVc3w2OizpcFZ/29/joGmhW9f7ZY7DD1b2M5v/bHBgAIEd kxwAKmMU/Q== 1.0 eAEzb5/BtqEz0mHq5ugfV29EOTzd9fjoNIZIh6JaHVkN8xiHmgX99oenFDswQAEA JaASrg== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8IAA72BPw= eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFjYAACAAAFAAE= 1.0 eAFjYAACAAAFAAE= 1.0 eAFzyk+p/J8EIv4xgCAARPsINQ== eAFzL0qs/P+PAQYBPcYHiQ== 1 eAEdyzEKgDAMBdAbZfME6mzBwbGE9ouFaEqbeH0V58ebbxZnK3pNbEyhaUL2hk4r BMmCVpff40CLW3XrtB0qGCHS417E0JApf70YzucFLQggoA== 1 PopulationSelectPopulation_5 eAFLzs/JLypOLXEoSszMS8ov//+PAQIBkJwLlQ== eAFjYGRgYAAAAAkAAg== 1.0 eAFzyk+p/J8EIv4xgCAARPsINQ== colorset@rainbow body 1 colorset@rainbow Body Body 0 1 1 Filtered Filtered Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation_5.Outputs.WholeCells_filtered.dataitem 3.0 0 PopulationSelectPopulation_5 1 Nuclei Select a population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAEdyzEKgDAMBdAbZfIG6mzBwVFC+8FCNKVJvL6K8+PNN0uwV70mdqbUNaNEh9EK QfakLeT3faAlvIUbbYcKRogYlW9Vx/m8GWkc8Q== eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAELTs1JTS4JyC8ozUksyczPizf5DwBMegge eAHjBgAADAAM 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf6DwDIKg0T eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 SelectPopulation_5 0 1 UI.InputParameters.SelectPopulation_5.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzK03OSc3875iXl1qRmRcWn5earpCcmpNT/B8AlWELUg== Filter by Property Select a method 0 Complex s Method Method Discrete 0 SelectPopulation_5 0 1 UI.InputParameters.SelectPopulation_5.Method.title 1 eAFzy8wpSS1SSKpUCCjKL0gtKqn8DwBG/Afm Filter by Property eAFzy8wpSS1SSKpUCCjKL0gtKqn875yfm5ufp+AGlin+75OZl5pYpOCck1hcnJmW mVr0Pzg1JzW5BKTHN7E4+z8Anq0bzA== 0.0 Define a proper value for the selection criterion 0 Complex d Attribute1 Numeric 0 SelectPopulation_5 0 0 UI.InputParameters.SelectPopulation_5.Method.Attribute1 1 0 0 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName1, RegionTable=ScalarTable| ScalarTable_1=TableForSelectedRegion, SelectedScalarRowIndex_1=SelectedRegionRowIndex) Complex s AttributeName1 Discrete _Scalar5>=140 3 3 eAGLdzeM/x/vbgQijOP/AwAxDQaj 0 SelectPopulation_5 0 0 eAHzy89L/Q8ABloCkA== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA UI.InputParameters.SelectPopulation_5.Method.Attribute1.label 1 eAH7DwABAAEA eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP08hxDfIlzhFwSUpLqll/8Eagl30CRnsmJNakahgZmIOMd0xLy+1 IjMvLD4vNV0hOTUnp/g/AOhfRvU= > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier1 Discrete 0 SelectPopulation_5 0 0 UI.InputParameters.SelectPopulation_5.Method.Attribute1.qualifier 1 eAGz+w8AAX0BPg== > eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB 0.0 Define a proper value for the selection criterion 0 Complex d Attribute2 3.00000000000000018e+34 -3.00000000000000018e+34 Numeric 0 SelectPopulation_5 0 0 UI.InputParameters.SelectPopulation_5.Method.Attribute2 1 0 140 eAHjur64wLYrwiENBNqiHBhAoCEGSsc5nD0DBDYJDrNmAoFlIpROgqg3S4bSKQ7G YJAK0WeQBqXTIfp1MhwAch0jOg== 1.0 7.93700000000000028 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName2, RegionTable=ScalarTable| ScalarTable_2=TableForSelectedRegion, SelectedScalarRowIndex_2=SelectedRegionRowIndex) Complex s AttributeName2 Discrete 0 SelectPopulation_5 0 0 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= eAELTk7MSSz6DwALOQNW eAGLD05OzEksMv0PABFSA+o= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzTsxJLs1JLEn1zCtJzSvOLKkMKMovSC0qyUwtjjf6DwDIKg0T eAEzNPgPAAH1AWE= eAH7DwABAAEA eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cMxJrUhUMDMx/w8AT34UnQ== eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAFzzEmtSFQwMzH/DwASlwOs eAEz+A8AAWEBMA== eAFzzi/NKynWD8isSM35DwAjwwWt eAHzTU3MU/DMK0nNK84sqfwPACxsBmg= eAHzTU3MU8jMK0nNK84sqVTIT1NwrizJL8hJLM5VCEpNz8zPU0iqVEjOSMzLS81R cMxJrUhUMDMx/w8AT34UnQ== eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjYWBgAAAAFAAF 1.0 5.0 UI.InputParameters.SelectPopulation_5.Method.Attribute2.label 1 eAHzzCtJzSvOLKlUcK4syS/ISSzOVQhKTc/Mz1NwzEmtSFQwMzFX8E1NzPsPAFQh D5Q= Intensity Cytoplasm Region Alexa 647 Mean eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP08hxDfIlzhFwSUpLqll/8Eagl30CRnsmJNakahgZmIOMd0xLy+1 IjMvLD4vNV0hOTUnp/g/AOhfRvU= > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier2 Discrete 0 SelectPopulation_5 0 0 UI.InputParameters.SelectPopulation_5.Method.Attribute2.qualifier 1 eAGzs/0PAAI2AXs= >= eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB 0.0 Define a proper value for the selection criterion 0 Complex d Attribute3 Numeric 0 SelectPopulation_5 0 0 UI.InputParameters.SelectPopulation_5.Method.Attribute3 1 0 0 Property by which the filtering criterion is defined 0 AAL::GetSelectedRegion(SelectedLabel=AttributeName3, RegionTable=ScalarTable| ScalarTable_3=TableForSelectedRegion, SelectedScalarRowIndex_3=SelectedRegionRowIndex) Complex s AttributeName3 Discrete 0 SelectPopulation_5 0 0 eAHzy89L/Q8ABloCkA== eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA UI.InputParameters.SelectPopulation_5.Method.Attribute3.label 1 eAH7DwABAAEA eAH775lXkppXnFlSqeBXmpyTWlqs4OHv6uwRHKJgbGxsYqTgm5qY9x+hyLmyJL8g J7E4VyEoNT0zP08hxDfIlzhFwSUpLqll/8Eagl30CRnsmJNakahgZmIOMd0xLy+1 IjMvLD4vNV0hOTUnp/g/AOhfRvU= > Define a proper qualifier for the selection criterion 0 eAGz+/+oc+l/GyC55L8tkFzwHwB2ywzc Complex s AttributeQualifier3 Discrete 0 SelectPopulation_5 0 0 UI.InputParameters.SelectPopulation_5.Method.Attribute3.qualifier 1 eAGz+w8AAX0BPg== > eAGz+29n+9/mv43tf1vb/4q2/wFFUAhB Nuclei Selected Name of the output population. 0 Complex s OutputName Output Population Numeric 0 Nuclei Selected Nuclei Selected 1 0 SelectPopulation_5 0 1 UI.InputParameters.SelectPopulation_5.OutputName 1 0 AnnexinV_pos cell 124082 122842 4.0 1 eAFzzMtLrcjMC4svyC9WSE7NyfkPAEMdB5g= 4.0 5.0.1.124082 0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAELSk3PzM/7/48BAgE6agdc eAHzK03OSS0t/h8flJqemZ9nBGMYwxgmMIbpfwC2LBO1 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAFzy8xL8StNzknN/O8GZDpXluQX5CQW5/7/x/iPMTg1JzW5xDk1JycoNT0zP+8/ ANRdFF4= eAEz+W/6/x/jP0az/wAfCQWb eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== eAH775yak6OQVKmQnJGYl5eao+CcmJNcmpNYkpqi4JmbmJ6qo5CbWpKRn6Lg9B+i NL8oJbWIWB2VJfkFOYnFuUSq/w8AB+c1/g== eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmfZ636HwBf fxbe eAH7/x8IAA72BPw= eAHzyE9NziguUfBNLM7+75yYk1yak1iSmqLgmZuYnvr/H+M/xv8AAJwPaw== eAEz+P+PAQIBHrkFKA== eAH7/x8IAA72BPw= eAH7/x8IAA72BPw= eAH7/x8IAA72BPw= eAFL+f+PAQIBIMEFXA== eAH7/x8IAA72BPw= eAH7/x8IAA72BPw= 5.0 Output Population AnnexinV_pos cell eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t KSgtKdYrAQvopQDVZ5ak5v53JdIInAYAAE71M+I= eAFjYAAAAAIAAQ== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjYQEAAA4ACQ== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAH7/x8AAv8B/w== eAH7/x8AAv8B/w== eAFjYAAAAAIAAQ== 1.0 eAH7/x8AAv8B/w== eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAGLj3dOzEn2zE1MTy12ADFLcxJLUlMcjAzAYv/jkeU98lOTM4pLgJK+icXZ/wGt rxcA eAH7/x8AAv8B/w== eAFjZAQAAAUAAw== 1.0 eAGNzEEOQEAMheEbNeIEEiQshBtMmpkGMYxo6/oMCzYWtv97+codvaKMYSlQELot WHK6EUOO3qpHoXrGnkwKrcqqwmDMNd2Vs+flsjS5G7gIjULzUf60v+UqkB1YItsg T696ArgNRDU= eAFjZAQAAAUAAw== 1.0 eAFzTsxJLs1JLElNUfDMTUxP/e+Rn5qcUVyi4JtYnP0fALbNDG4= eAFzTsxJLs1JLEn1zE1MT403+u+MIvAfANMQDXI= eAFjYgYAAAkABg== 1.0 eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAGVkN0KgkAQhV9lH6CrqAcIQRDKgqAuh2kbbGucEXe1fHpTUa9C6GqYcw7f/ACk lGFwNR1rKhkb8hGyrRgDJTlmBOsWIEVZtJcRi4A2rSyTaxNxwSGbA/rXyoziXt/G qoQSfTB6e5INfjbHTOzkHjVBC0afg2OufJfvL5rQY3Cp7ETo4+QCQpmxxOy7pa8P ZRoaiLVMZrLKmTojnLTo/+RUYDPN+gGapUL9wP4PvW2/b/6xiQ== eAGdj8EKwkAMRPWPSr+gUigUdL3pMWzXWKMhC7tp9eu7Vj24ggfxNgl5MxODvVUa kZiHqGHWXpKx5mOeFtPSDI6RUiukZHlj4yWt/bX2MkNRt90Znca0P3nGGplzCW+z Eb+f/LmFI7FiwMOLd49gaHxo82+yTgDVSgRvJDsQ7KuyeCJFFv+T5R0orYef eAGNTUkKgDAQU1/SvwiCoPXoUWoZtDp0wI76+9YFQXuTHJKQhEgYFJsNSsTV8XJq skEqG3mf+FSuGsGE0ho2CkWt3Bwq2kVO9pw5Fk0/gWYXfNaOhJAD4mW+eIPulvrq dAUt0dvzFNO/6QGY/1qv eAG1lMFOwzAMht+omgbch8omVYJtAgmOkWlNMbhJlTiFPT2km0QRFEjbcczBXz77 d7JsgD0IGX0BAsnWmhwLb9ElKXDuGQSzCkpU82TjpfbikjWWoaBBYvZO7L44KUI1 CVZvyxFAWE9jHVmtww0XW5Eu1j5npI5yOEZMqKc40yQEfAXuOWbGPYRL85IaHXJy srl/wjwkGKmS7sTUDK76aOXu0TCmyHwUhupSbzBG6gY5+Lf3X2MZVnakV7fZWlA7 kl1Y+xqtEDp18g/QqchPemdTWVlPz93bHhbwIY+tqdt/IuShTkfaRYHUA7GgxSJ+ VX5Wy9slVitjs4G/2DfVsYn8AlJqca41vpK+VRrLxXy2152NarzPb9oovxD/GOU7 S12JzA== eAFjYEADjIyMDAAAHgAE 1.0 eAFzTsxJLs1JLEn1zE1MT403+v+PwRlF6P8/RrfMvBS/0uSc1Mz//5hAECTgXFmS X5CTWJwLFYPIBKfmpCaXBOQXgMzMzM+LN/n/jwUoxYIhYfr/HysAi5U3zQ== eAGdj8EKwjAQRLX9o2q/ICD0IkU/IIS4lIVtEprtoT+/q8VDKoqIx3nDDDPGkZ/J MXSjG8A2KjvzglT2Jwy38+wJUKWSapVm4ZjI5VGlvgKBZwNEFxgwBi0FgSFk5KWf YoKJEbI9fLWLuYm0hXYfGht9LuhjWp88Ftjjv6h9D/6K7pB4jZI= eAFjYmJmZmFhYWVlY+fg5OLm5uYBAgAE4QCa 1.0 eAFjYMACAAAVAAE= 1.0 eAFjYmZiZmRmYWRiBAMgh4mZBQAB5AAr 1.0 eAFjYGBgYGVgYGNg5+Dk4ubhAXIZGAADEgBR 1.0 eAFjZAQDBkYGMGAE0oyMAACuAA0= 1.0 eAGtj00KwjAQhf25UTUnCAjdSNEDhBAfJTBNQjNd9PIZDS6iKK5kdt/jfbzRltxC ltFPdoTppGz0G5KyPflwOy+O4KXs6lWgV46JbJ6k7K8gONYgumD0MUhTBEbIntdh jgkze2Rz+Bm38KWiGu2/GDt5Lhhiqr88FpjjH5H6dCm5A45ghnM= eAH7////f7/8vFQg9Y8BiJAhlPsfAMCOFnE= eAH7/x8LAADmLhTs eAFjYMACAAAVAAE= 1.0 eAH7////f4/81OSM4hIF38TibCD3HwMQISBY4P9/AAc9GV4= eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA eAELKUrMzMvMS3dJLEn8DwAkdQW2 1 Method MethodFolder InputParameters.SelectPopulation_5.Method Method WholeCells_string Method 1 1 0 1 builtin Each FindSpots 1 Replace me 1 1 Legio SpotsFindSpots Population: Legio 0 sticky eAFzdXcL+A8ABOsCIg== eAELLsgv+R/k7/kfABT2BI8= Standard 0 eAELKMovSC0qyUwtVvBJTc/M/w8APSsHPQ== FeedBack.SpotsFindSpots eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 MaxValue:Plain eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 MaxValue eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Plain eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZGRgZGQEAAAXAAY= 1.0 eAFzL0qs/P+PwSczNxVIgSAAVd0JEQ== eAFzL0qs/P+PAQYBPcYHiQ== eAFjYGBkYGAAAAAKAAI= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYGBkYGAAAAAKAAI= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYACCC5McQBSD71aHBn67Hw8Ol0H4e5ZCaLmtENplsQMAD2oK3g== 1.0 eAEzb5/BtqEz0mHq5ugfV29EObjue3x0GkOkQ1GtjqyGeYxDzYJ++8NTih0YoAAA EhwSEg== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8AAv8B/w== eAFLzs/JLypOLXEoSszMS8ov/1+ekVmS+h8AdyUKvQ== eAFLzs/JLypOLXEoSszMS8ov/1+ekVmS+h8AdyUKvQ== eAFjZAQAAAUAAw== 1.0 eAELLsgv+R/k7/kfABT2BI8= eAFjZAQAAAUAAw== 1.0 eAFjYAAAAAIAAQ== 1.0 eAFzyi9KSS36nwShAC8BBts= eAFzL0qs/P+PwSczNxVIgSAAVd0JEQ== 1 eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEsJLsgvKdbzLy0pKAXSEF4K UF1mSWrufwCu8xeU eAFLzs/JLypOLXEoSszMS8ov//+PAQBREgib eAFjZAAAAAQAAg== 1.0 eAFLyk+p/O8EIgAaUQU7 colorset@rainbow body 1 colorset@rainbow Body Body 0 MaxValue:Plain Colormode Render MaxValue Images Plain Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov/1+ekVmS+l/Zzc3S2MDxPwDVrQ1I eAFjZGQEAAAJAAQ= 1.0 Stencils Render 10.0 eAFzyi9KSS36nwShivNzMlP+AwBhsgn1 1 1 Spots Spots Images=AAL::GetValidImages().v_images; Set(Stencils=AAL::GetImageViewStencilsMultiList2(vec("EvaluationData.Procedures.FindSpots.Outputs.Spots.dataitem","EvaluationData.Procedures.FindSpots.Outputs.olROIWithMain.dataitem"), vec("Spot","ROI")).v_stencils) Standard EvaluationData.Procedures.FindSpots.Outputs.Spots.dataitem 3.0 1 Spots spotmethodwizardFindSpots Detected Spots 0 sticky eAFzdXcL+A8ABOsCIg== eAELLsgv+R/k7/kfABT2BI8= Standard 0 eAELKMovSC0qyUwtVgguyC8p/g8APdUHZg== FeedBack.spotmethodwizardFindSpots eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 1 MultiDefaultOverLayMixed WeightedAverage script 0 Default Default 1 1 Default Default 0 0 Default Default 1 1 Default Default 0 0 eAFjZAQBAAAbAAc= 1.0 eAFzL0qs/P+PAQYBPcYHiQ== eAFjYGBkYGAAAAAKAAI= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== 1 eAELLsgv+R/k7/kfABT2BI8= eAFjZAQAAAUAAw== 1.0 eAFLzs/JLypOLXEoSszMS8ov/59elJqa9x8AdswKrQ== eAFLyk+p/O+UX5SSWvQfACTOBgs= eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEsJLsgvKdbzLy0pKAXSxUBe bmpJRn5KeWZVYlGKXgpQS2ZJau5/AM5fHFM= eAFLzs/JLypOLXEoSszMS8ov/w8AP9wHnQ== eAFjBAAAAgAC 1.0 eAFLyk+p/A8ABsgCrg== colorset@rainbow body 1 colorset@rainbow Border Border 0 1 1 spotmethodwizard spotmethodwizard Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils_MultiList(vec("EvaluationData.Procedures.FindSpots.Outputs.Spots.dataitem","EvaluationData.Procedures.FindSpots.Outputs.olROIWithMain.dataitem"), vec("Spot", "ROI")).v_stencils; Standard EvaluationData.Procedures.FindSpots.Outputs.spotmethodwizard.dataitem 3.0 1 Nuclei PopulationFindSpots Region where spots are searched and detected spots. 0 sticky eAFzdXcL+A8ABOsCIg== eAFzrizJL8hJLM5VCEpNz8zP+x9ckF9S/B8AcToKWA== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationFindSpots eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQBAAAbAAc= 1.0 eAFzL0qs/P+PAQYBPcYHiQ== eAFzL0qs/P+PAQYBPcYHiQ== eAFjYGBkYGAAAAAKAAI= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYAABAAAGAAE= 1.0 eAEBMADP/3ZxJp974NA/X5hOH1ujyz/X1fQ5YjPPP4B/PIkGS9E/YK1knGGW0D+Q +5FRaKDIPz1hF7M= 1.0 eAHrKZ/2P6V/goPhRLkVc3w2OizpcFZ/29/joGmhW9f7ZY7DD1b2M5v/bHBgAIEd kxwAKmMU/Q== 1.0 eAEzb5/BtqEz0mHq5ugfV29EOTzd9fjoNIZIh6JaHVkN8xiHmgX99oenFDswQAEA JaASrg== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8MABvrBvo= eAFLzs/JLypOLXEoSszMS8ov//+PAQEBxOQNkQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQEBxOQNkQ== eAFjYGBgYGRkAAAADAAD 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9z+4IL+k GEwqOOUXpaQWFf8HADLBHyw= eAFjYAADAAAHAAE= 1.0 eAFjYAADAAAHAAE= 1.0 eAFzyi9KSS36/48BBJMgnKT8lEqgCAC23w2A eAFzL0qs/P+PAQYBPcYHiQ== 1 eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEsJLsgvKdbzLy0pKAXS4Rn5 OanOqTk5xXopQMWZJam5/wEl2Blt 1 SpotsFindSpots eAFLzs/JLypOLXEoSszMS8ov//+PAQEBxOQNkQ== eAFjYGRgACIAABAAAw== 1.0 eAFzyi9KSS36nwSh/jH8Y0jKT6n8D2IAALdbDYA= colorset@rainbow border 1 colorset@rainbow Border colorset@rainbow Border Border 0 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.FindSpots.Outputs.WholeCells.dataitem 3.0 0 SpotsFindSpots 1 Alexa 647 Select channel 0 Complex s 1 1 channel_string Channel Discrete 1 FindSpots 0 0 UI.InputParameters.FindSpots.channel_string 1 eAFzdXcL+A8ABOsCIg== EGFP eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== None Select population for ROI 0 Complex s WholeCells_string ROI Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAEdyzEKgDAMBdAbZfME6mzBwVFC+8FCNKVJvL6K8+PNN0uwV70mdqbUNaNEh9EK QfakLeT3faAlvIUbbYcKRogYlW9Vx/m8GYcc8g== eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAELTs1JTS4JyC8ozUksyczPizf9DwBMfAgf eAHjAQAADQAN 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAELTs1JTS4JyC8ozUksyczPizf5DwBMegge eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 FindSpots 0 1 UI.InputParameters.FindSpots.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAHzy89L/e9XmpyTmvnfMS8vtSIzLyw+LzVdITk1J6cYIVSQXwwW+g8AGzwVeA== Cell Select ROI region 0 Complex s 1 Region Discrete 0 FindSpots 0 1 eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAELSk3PzM/7DwALcgNk eAGLD0pNz8zPM/0PABGZA/g= eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAELTs1JTS5xTs3JCUpNz8zP+w8AO58HRA== eAEz+w8AAW0BNg== eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAH7DwABAAEA eAFzrizJL8hJLM5VCEpNz8zPs1b9DwBKGAeg eAH7DwABAAEA eAH7DwABAAEA eAEz+A8AAWEBMA== eAH7DwABAAEA eAH7DwABAAEA eAH7DwABAAEA eAFL+Q8AAckBZA== eAH7DwABAAEA eAH7DwABAAEA eAFjYWBgAAAAFAAF 1.0 5.0 UI.InputParameters.FindSpots.Region 1 eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== Cytoplasm Region eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9x8AM4YW fg== A corrected Select a method 0 eAFz/O/03/m/y38AFSAFBw== Complex s Method Method Discrete eAErLsgvyU0tychPKc+sSixKccvMSwkGihX/BwCU+Qty 0.5 0.5 0 1 0.5 0.5 0.82 0.03 1 0 0 FindSpots 0 0 UI.InputParameters.FindSpots.Method.title 1 eAFzUkjOLypKTS5JTfkPABwKBR0= B corrected eAFzVEjOLypKTS5JTfnvhMR2RmK7ILEB4BcUcw== 0.03 Threshold value for relative spot intensity 0 Complex d MinRelativeSignal Relative Spot Intensity 1.0 0.0 Numeric 0 FindSpots > 1 0 0.005 UI.InputParameters.FindSpots.Method.MinRelativeSignal 0 1 0.03 eAGrFlnn/rBqiv0OudbXgTvm2VeD+Uug9BZ7YzA4bD9rJgictAcAJKIWRg== 1.0 0.05 0.5 Threshold value for Detection Sensitivity 0 Complex d DetectionSensitivity Detection Sensitivity 1.0 0.0 Numeric 0 FindSpots 0 0 0.05 UI.InputParameters.FindSpots.Method.DetectionSensitivity 1 1 0.82 eAGbNRMETtobg8Fl+1lg/k17BjB4ABV/DBV/aQ8A6rQTxQ== 1.0 0.05 1 Splitting sensitivity 0 Complex d SplittingCoefficient Splitting Sensitivity 1.0 0.0 Numeric 0 FindSpots 1 0 0.005 UI.InputParameters.FindSpots.Method.SplittingCoefficient 0 1 1 eAFjYACBB/ZpYPDMftZMEHhpf/YMCLyBir+zBytj+GAPAOC0FBw= 1.0 0.05 0.5 Splitting sensitivity 0 Complex d SplittingSensitivity Splitting Sensitivity 1.0 0.0 Numeric 0 FindSpots 0 0 0.005 UI.InputParameters.FindSpots.Method.SplittingSensitivity 1 1 0 eAGbNRMETtobg8Fl+1lg/k17BjB4ABV/DBV/aQ8A6rQTxQ== 1.0 0.05 0.5 Tuning parameter for selecting background correction, range 0..1. Value 0 yields nearly constant background. 0 Complex d dBackGroundCorrection Background Correction 1.0 0.0 Numeric eAHLzHXOLypKTS5JTfHMTUxPdcvMSwkuyC8p/g8mEdyA/ILSnMSSzPw8hBgAFnMa ZA== 0 FindSpots 1 0 0.005 UI.InputParameters.FindSpots.Method.More.dBackGroundCorrection 0 1 0.5 eAFjYICAWTNB4KQ9hL5pDxF9YG8MBo+h4i+h4h/sAQL6ErA= 1.0 0.05 1 Calculate additional spot properties and regions 0 Complex b AddPropertiesAndRegions Calculate Spot Properties 1 0 Numeric 0 FindSpots 0 1 1 UI.InputParameters.FindSpots.Method.More.AddPropertiesAndRegions 1 0 1 1 Spots Name of the output population of spot-like features 0 Complex s OutputName Output Population Numeric 0 Spots Spots 1 0 FindSpots 0 1 UI.InputParameters.FindSpots.OutputName 1 0 Legio 124082 122842 13 1 eAHzSU3PzP8PAAh7AvA= 13 5.0.1.124082 0 Output Population Legio eAGLjy/xzE1MT3VOzEkuzUksSQXz4o3+x2OV+A8A0DoTnA== eAEr8cxNTE/9/48BABQ2BFU= eAH7/x8AAv8B/w== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nNOzEkuzUksSfXMTUxPjTfS8y8t 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eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA 1 Method MethodFolder InputParameters.FindSpots.Method Method Region Method 0 0 0 MoreFolder InputParameters.FindSpots.Method.More SplittingSensitivity 0 0 0 1 builtin Each CalculateProperties 1 Replace me 1 1 Nuclei PopulationCalculateProperties Population: Nuclei 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzTs3J+Q8ABgMCgA== Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationCalculateProperties eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 eAFjAAAAAQAB 1.0 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 eAFjAAAAAQAB 1.0 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 eAFjAAAAAQAB 1.0 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 eAFjAAAAAQAB 1.0 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQBAAAbAAc= 1.0 eAFzL0qs/P+PwSczNxVIgSAAVd0JEQ== eAFzL0qs/P+PAQYBPcYHiQ== eAFjZGBkYGAAAAAQAAM= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYGBkYGAAAAAKAAI= 1.0 eAEBMADP/3ZxJp974NA/X5hOH1ujyz/X1fQ5YjPPP4B/PIkGS9E/YK1knGGW0D+Q +5FRaKDIPz1hF7M= 1.0 eAHrKZ/2P6V/goPhRLkVc3w2OizpcFZ/29/joGmhW9f7ZY7DD1b2M5v/bHBgAIEd kxwAKmMU/Q== 1.0 eAEzb5/BtqEz0mHq5ugfV29EOTzd9fjoNIZIh6JaHVkN8xiHmgX99oenFDswQAEA JaASrg== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8MABvrBvo= eAFLzs/JLypOLXEoSszMS8ov//+PAQEBxOQNkQ== eAFLzs/JLypOLXEoSszMS8ov//+PAQEBxOQNkQ== eAFjYGQAAQAADQAC 1.0 eAHzK03OSS0t/u+cmpPz3zc1N6koMS/1v3NlSX5BTmJxLoKlEJSanpmf9z+4IL+k GEwqOOUXpaQWFf8HADLBHyw= eAFjYAADAAAHAAE= 1.0 eAFjYAADAAAHAAE= 1.0 eAFzyi9KSS36n5SfUvn/HwMMAgB9FgsB eAFzL0qs/P+PwSczNxVIgSAAVd0JEQ== 1 eAENy0EOgCAMBMAf9RPoWW+eG9hEkkoJbP2+cp1k9lctlNXbplQ5h2eUGJiS1HKY Ess6BuuyI9iDU67bDQlmU8pqlXi+HzwbHY4= eAFLzs/JLypOLXEoSszMS8ov//+PAQEBxOQNkQ== eAFjYGQAAQAADQAC 1.0 eAFzyi9KSS36n5SfUvn/HwMMAgB9FgsB colorset@rainbow body 1 colorset@rainbow Border Border 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQGV3dwsjQ0c/wMAOhsQHA== eAFjYGQAAUYAABAAAw== 1.0 Stencils Render 10.0 eAFzyi9KSS36n5SfUvn/HwMMFufnZKb8BwDIqw4b 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.CalculateProperties.Outputs.WholeCells.dataitem 3.0 0 PopulationCalculateProperties 1 Nuclei Select a population 0 Complex s WholeCells_string Population Discrete eAHzK03OSc38DwALhgNg eAELz8jPSXVOzckp/g8AGkYE8g== eAHzK03OSc38DwALhgNg eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEsJLsgvKdbzLy0pKAXS4Rn5 OanOqTk5xXopQMWZJam5/wEl2Blt eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xLCS7ILyn+DwAVwgSa eAHjBQAADgAO 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFjAAAAAQAB 1.0 eAFjAAAAAQAB 1.0 eAELTs1JTS4JyC8ozUksyczPizf9DwBMfAgf eAHzy89L/Q8ABloCkA== eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAHzyE9NziguUfBNLM7+DwAifQV6 eAHzT8pKTS4p/g8ADnADyg== 0 CalculateProperties 0 1 UI.InputParameters.CalculateProperties.WholeCells_string 1 eAHzK03OSc38DwALhgNg Nuclei eAFzzMtLrcjMC4vPS01XSE7NySn+7wgTKsgvBgv99ytNzknN/O+Tmp6Z/x8ADj0V 2A== By Formula Select a method 0 Complex s Method Method Discrete Formula Formula 0 CalculateProperties 0 1 UI.InputParameters.CalculateProperties.Method.title 1 eAFzqlQISs1JLElNUQjILygFsjLz8/4DAFspCOc= By Related Population eAFzqlRwyy/KLc1J/O9UqRCUmpNYkpqiEJBfABQpyczP+w8A2eMNlw== AnnexinV_neg cells 0 Complex s WholeCells_related_string Related Population Discrete eAHzSU3PzP8PAAh7AvA= eAELLsgvKf4PAAklAxk= eAELLsgvKf4PAAklAxk= eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEsJLsgvKdbzLy0pKAXSEF4K UF1mSWrufwCu8xeU eAFjBAAAAgAC 1.0 eAFzy8xLCS7ILyn+DwAVwgSa eAFzy8xLCS7ILyn+DwAVwgSa eAHjBQAADgAO 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xLCS7ILyn+DwAVwgSa eAHzK03OSc38DwALhgNg eAFzrizJL8hJLM5VCEpNz8zP+w8APJsHQA== eAFjAAAAAQAB 1.0 eAH7DwABAAEA eAHzT8pKTS4p/g8ADnADyg== 0 CalculateProperties 0 1 UI.InputParameters.CalculateProperties.Method.WholeCells_related_string 1 eAHzSU3PzP8PAAh7AvA= Legio eAFzzMtLrcjMC4vPS01XSE7NySn+7wgTKsgvBgv990lNz8z/DwCBrxJ5 0 Carry a property Number of Legio to population Nuclei 0 Complex b NumberOfObjectsFromPop2__donotuse Number of Legio 1 0 Numeric 0 CalculateProperties 0 1 1 UI.InputParameters.CalculateProperties.Method.NumberOfObjectsFromPop2__donotuse 1 0 1 1 0 Carry a property Relative Spot Intensity to population Nuclei 0 Complex b __X@Relative@20Spot@20Intensity Relative Spot Intensity 1 0 Numeric 0 CalculateProperties 0 1 1 UI.InputParameters.CalculateProperties.Method.__X@Relative@20Spot@20Intensity 1 0 0 1 Mean Select a statistic 0 Complex s __X@Relative@20Spot@20Intensity_stat Discrete 0 CalculateProperties 0 1 UI.InputParameters.CalculateProperties.Method.__X@Relative@20Spot@20Intensity.qualifier 1 eAHzTU3M+w8ABhgCgQ== Mean eAHzTU3M+x9ckuKSWvbfOUxB9X9wae5/38SK/76Zef8BxxgOIw== 0 Carry a property Corrected Spot Intensity to population Nuclei 0 Complex b __X@Corrected@20Spot@20Intensity Corrected Spot Intensity 1 0 Numeric 0 CalculateProperties 0 1 1 UI.InputParameters.CalculateProperties.Method.__X@Corrected@20Spot@20Intensity 1 0 0 1 Mean Select a statistic 0 Complex s __X@Corrected@20Spot@20Intensity_stat Discrete 0 CalculateProperties 0 1 UI.InputParameters.CalculateProperties.Method.__X@Corrected@20Spot@20Intensity.qualifier 1 eAHzTU3M+w8ABhgCgQ== Mean eAHzTU3M+x9ckuKSWvbfOUxB9X9wae5/38SK/76Zef8BxxgOIw== 0 Carry a property Uncorrected Spot Peak Intensity to population Nuclei 0 Complex b __X@Uncorrected@20Spot@20Peak@20Intensity Uncorrected Spot Peak Intensity 1 0 Numeric 0 CalculateProperties 0 1 1 UI.InputParameters.CalculateProperties.Method.__X@Uncorrected@20Spot@20Peak@20Intensity 1 0 0 1 Mean Select a statistic 0 Complex s __X@Uncorrected@20Spot@20Peak@20Intensity_stat Discrete 0 CalculateProperties 0 1 UI.InputParameters.CalculateProperties.Method.__X@Uncorrected@20Spot@20Peak@20Intensity.qualifier 1 eAHzTU3M+w8ABhgCgQ== Mean eAHzTU3M+x9ckuKSWvbfOUxB9X9wae5/38SK/76Zef8BxxgOIw== 0 Carry a property Spot Contrast to population Nuclei 0 Complex b __X@Spot@20Contrast Spot Contrast 1 0 Numeric 0 CalculateProperties 0 1 1 UI.InputParameters.CalculateProperties.Method.__X@Spot@20Contrast 1 0 0 1 Mean Select a statistic 0 Complex s __X@Spot@20Contrast_stat Discrete 0 CalculateProperties 0 1 UI.InputParameters.CalculateProperties.Method.__X@Spot@20Contrast.qualifier 1 eAHzTU3M+w8ABhgCgQ== Mean eAHzTU3M+x9ckuKSWvbfOUxB9X9wae5/38SK/76Zef8BxxgOIw== 0 Carry a property Spot Background Intensity to population Nuclei 0 Complex b __X@Spot@20Background@20Intensity Spot Background Intensity 1 0 Numeric 0 CalculateProperties 0 1 1 UI.InputParameters.CalculateProperties.Method.__X@Spot@20Background@20Intensity 1 0 0 1 Mean Select a statistic 0 Complex s __X@Spot@20Background@20Intensity_stat Discrete 0 CalculateProperties 0 1 UI.InputParameters.CalculateProperties.Method.__X@Spot@20Background@20Intensity.qualifier 1 eAHzTU3M+w8ABhgCgQ== Mean eAHzTU3M+x9ckuKSWvbfOUxB9X9wae5/38SK/76Zef8BxxgOIw== 0 Carry a property Spot Area [px²] to population Nuclei 0 Complex b __X@Spot@20Area@20@5bpx@c2@b2@5d Spot Area [px²] 1 0 Numeric 0 CalculateProperties 0 1 1 UI.InputParameters.CalculateProperties.Method.__X@Spot@20Area@20@5bpx@c2@b2@5d 1 0 1 1 Mean Select a statistic 0 Complex s __X@Spot@20Area@20@5bpx@c2@b2@5d_stat Discrete 0 CalculateProperties 0 1 UI.InputParameters.CalculateProperties.Method.__X@Spot@20Area@20@5bpx@c2@b2@5d.qualifier 1 eAELLs39DwAEiAI1 Sum eAHzTU3M+x9ckuKSWvbfOUxB9X9wae5/38SK/76Zef8BxxgOIw== 0 Carry a property Region Intensity to population Nuclei 0 Complex b __X@Region@20Intensity Region Intensity 1 0 Numeric 0 CalculateProperties 0 1 1 UI.InputParameters.CalculateProperties.Method.__X@Region@20Intensity 1 0 0 1 Mean Select a statistic 0 Complex s __X@Region@20Intensity_stat Discrete 0 CalculateProperties 0 1 UI.InputParameters.CalculateProperties.Method.__X@Region@20Intensity.qualifier 1 eAHzTU3M+w8ABhgCgQ== Mean eAHzTU3M+x9ckuKSWvbfOUxB9X9wae5/38SK/76Zef8BxxgOIw== 0 Carry a property Spot to Region Intensity to population Nuclei 0 Complex b __X@Spot@20to@20Region@20Intensity Spot to Region Intensity 1 0 Numeric 0 CalculateProperties 0 1 1 UI.InputParameters.CalculateProperties.Method.__X@Spot@20to@20Region@20Intensity 1 0 0 1 Mean Select a statistic 0 Complex s __X@Spot@20to@20Region@20Intensity_stat Discrete 0 CalculateProperties 0 1 UI.InputParameters.CalculateProperties.Method.__X@Spot@20to@20Region@20Intensity.qualifier 1 eAHzTU3M+w8ABhgCgQ== Mean eAHzTU3M+x9ckuKSWvbfOUxB9X9wae5/38SK/76Zef8BxxgOIw== per Cell Common prefix for calculated properties 0 Complex s RelPop_PropertyName Output Properties Numeric 1 per Cell per Cell 0 0 CalculateProperties 0 1 UI.InputParameters.CalculateProperties.RelPop_PropertyName 1 0 per Cell 124082 122842 5 1 5 5.0.1.124082 0 Number of Legio eAELSs1JLMksS1UILsgvUfDMK0nNK84sqfzvnF9UlJpckpqCLhGal4wqFZCamI2k EWyOc35eSVFiccl/MM8pMTk7vSi/NC8FXZ1jUWqiQnRBxaFNsf+DUtMz8/PQVZTk K2BIAAD3XEqJ eAELTk7MSSz6/48BGQIAcX0KSA== eAGLD05OzEksMvwfD2EYwRjGMIYJjGEKY5jBGOYwhsV/AGIoH0U= eAELLsgv+f+PARkCAGM8CZg= eAFzy8xLCS7ILyn+/48BGQIAjb4LjA== eAEzNP7/jwEZAgBNAwhW eAELLsgv+Q8E/xj+MQSnJhYlZygEpaZn5uf9/w8A0TsOdw== eAELSs1JLMksS1UILsgvUfDMK0nNK84sqfzvnF9UlJpckpqCLhGal4wqFZCamI2k 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eAH7////f7/8vFQg9Y8BiJAhlAuk/EqTc1IzwWoAEz8dyg== eAH7/x8bcK4syS/ISSzOVQhKTc/MzwOpAQAK4yAn eAFjYMAFAAAaAAE= 1.0 eAH7////f4/81OSM4hIF38TibCD3HwMQISBYAE4AAM2xHlc= eAHzyU9OzFFwSSxJVPArTc5JzfwPADv7BwU= eAFzLCkpCklMykn9DwAV2ASD eAHzyU9OzPErTc5JzQxJTMpJ/Q8AO3YHMw== eAFzLUvMKU0syczPc0ksSdQLKMpPTk0pLUot1nPLzEvxK03OSc3U8y8tKSgtKdZz LCkpCklMyknVSwEqzixJzf0PACHoGUU= eAFjAAAAAQAB 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAFzy8xL8StNzknN/A8AGaQE4Q== eAFjAQAABQAF 1.0 eAFjAAAAAQAB 1.0 eAFjAgAAAwAD 1.0 eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAFzy8xL8StNzknN/A8AGaQE4Q== eAH7DwABAAEA eAH7DwABAAEA eAFjAAAAAQAB 1.0 eAH7DwABAAEA 1 Method MethodFolder InputParameters.CalculateProperties.Method Method WholeCells_string Method 1 1 0 1 builtin Each SelectPopulation 1 Replace me 1 1 Nuclei PopulationSelectPopulation Population: Nuclei 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAELTs1JTS5JTVHwT8oCMor/u2QWJycWpSCJAAD5nw5/ Standard 0 eAELKMovSC0qyUwtVvArTc5JzfwPAESDB60= FeedBack.PopulationSelectPopulation eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 eAFjAAAAAQAB 1.0 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.0 eAHLy89L/Z9elJnyv7ikKLMgtfh/cX4OkAcAl/MMeA== input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__SetScaleBar 0 1 eAFjAAAAAQAB 1.0 0 s Show Scale eAHLyy/KTcz5/48BAgE8qweB eAHzy89L/e+UX1KSn6vgk5pW8j8kvwDBCMpMzyiBSUM4ABF1FW4= 1 0 0 NaN eAFjYAACAAAFAAE= 1.0 eAFjYACBH/VgCk58sIcyHSA0hwMASRYDLw== 1.0 input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadSharpnessFrames -4 0 1 eAFjAAAAAQAB 1.0 1 0 d Sharpness 3 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -5 0 1 eAFjAAAAAQAB 1.0 true 0 b Apply Sharpness 3 1 true input replace 0 0 0 0 value 0 input text 0 1 input replace 0 0 0 0 value 0 input text 0 0 container width:0 simple 0 0 1 container container 10.0 1 0 container simple 0 0 1 container container General controls 1 Channels eAFjZAQBAAAbAAc= 1.0 eAFzL0qs/P+PAQYBPcYHiQ== eAFzL0qs/P+PAQYBPcYHiQ== eAFjYGBkZGAAAAANAAM= 1.0 eAFzzEmtSFQwMzH/n1IclJry39XdLeC/h7+rs0dwiIKxsbGJ0X/nxJzk0pzEktQU Bc/cxPTU/x75qckZxSUKvonF2f8BKF4ZVw== eAFjYAABAAAGAAE= 1.0 eAEBMADP/zvkc/gSUM4/Jj/yFCF90z/MQWm/vqThP0a3RomGmMw/4KhrWDdd1z/y +zf7G5rIP0UPGJE= 1.0 eAFbFaG0Ksx+msPXxfJ/X4R2O2x6KtMm9CvBQaix1uCJ0QaHaNfGORMfT3NgAIGg DQ4AJqAUag== 1.0 eAHLLr0VO7Ur0iHmAZP4joBohzkmxz7f4o506N49/8FntxiHya9+9e7cWOzAAAUA MJ4Uig== 1.0 eAFjYACBD/ZgiggaAJryBxs= 1.0 eAFjYACBB/ZgiggaAJKyBrs= 1.0 eAFjYCANAAAAMAAB 1.0 11 Regions eAH7/x8GADbTCfc= eAFLzs/JLypOLXEoSszMS8ov//+PAQHTi1JT8/4Xpab8V3ZzszQ2cPwPAAZkFWY= eAFLzs/JLypOLXEoSszMS8ov//+PAQHTi1JT8/4Xpab8V3ZzszQ2cPwPAAZkFWY= eAFjYAADRkZGAAAQAAQ= 1.0 eAFNijsOgCAQRK/CWcTCRk30BAgTxSxiWCg8/UpssJjJm89ULKGwaBDJiLAlc0H0 k+NNhkMjtWD38ZL1jpk/V11MDqkGEGyGU/N2VmDpPVtTt9YMfj+o6v96AVGpNfQ= eAFjYIABAAAKAAE= 1.0 eAFjYIACRgAACwAC 1.0 eAFzyi9KSS36/48BAZPyUyr//2Mszs/JTPkPAARJEBg= eAFzL0qs/P+PAQYBPcYHiQ== 1 eAElzEsKgDAMANEb5RQquLLgwqWENmAhmpKP19ei6zfMeCMHepVrQEdIKplKKBms xJQ9SQv+HJbwFm6wHdKFmG2fRGfmMNc/Kf1Rnc7nBeCqIw4= eAFLzs/JLypOLXEoSszMS8ov//+PIb0oNTXvf1Fqyn8AwWAN5Q== eAFjYGBkBAAABwAD 1.0 eAFzyk+p/P+PIQlMMQIANC4HOA== green body 1 colorset@rainbow Body colorset@rainbow Body Body 0 WeightedAverage:Extended Colormode Render WeightedAverage Images Extended Render OverlapControl none Render ScaleBar Render NaN eAFLzs/JLypOLXEoSszMS8ov//+PAQHTi1JT8/4Xpab8V3ZzszQ2cPwPAAZkFWY= eAFjYAADRkZGAAAQAAQ= 1.0 Stencils Render 10.0 eAFzyi9KSS36/48BAZPyUyr//2Mszs/JTPkPAARJEBg= 1 1 Population Population Images=AAL::GetValidImages().v_images; Stencils=AAL::GetImageViewStencils(ListRefString).v_stencils; Standard EvaluationData.Procedures.SelectPopulation.Outputs.Wholecells_ForIllustration.dataitem 0 UI_in=UI; UI=ocnt(); UI::C_Layout("(1)", vec("Controls"), cellalign="center", class="packer") Buttons=UI; UI=UI_in; 3.0 1 Infected FilteredSelectPopulation Population: Infected 0 sticky eAFzzEmtSFQwMzH/DwASlwOs eAFzTs3J+Q8ABgMCgA== Standard 0 eAELKMovSC0qyUwtVvDMS0tNLklN+Q8AU34Ibw== FeedBack.FilteredSelectPopulation eAFjAAAAAQAB 1.0 eAFjBAAAAgAC 1.0 eAELLs3NTSyq/A8AD00D7g== eAFjBAAAAgAC 1.0 0 SingleDefaultOverLay WeightedAverage script 0 1 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_Coloring_Setmode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAELLknMS0ksSvnvmpeRmJecmvLfIzM9IweIS/4DALJdDN0= 1 0 0 WeightedAverage:Extended eAFjYGAAAAADAAE= 1.0 eAHzTawIS8wpTbUKyEnMzPsfnpqZnlGSmuJYllqUmI5L1LWiJDUvJTXlPwAb/hnA input replace 0 0 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Coloring mode eAHLyy/KTcz5/4/hHwMAIKMFhQ== eAFzTEksKMksS/3vllmRmvI/KDW9NCexSME5Pye/6D8AvyMNDQ== 1 0 0 WeightedAverage eAFjYGAAAAADAAE= 1.0 eAELT81MzyhJTXEsSy1KTE/9H1ya+983sSIsMac09T8AuiwNQg== input replace 0 1 value 0 input menu 0 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB_Render_Images_SwitchMode 0 1 eAFjAAAAAQAB 1.0 0 s Color palette eAHLyy/KTcz5/48BABWZBIc= eAFzrShJzUtJTfkfnJlbkJP6HwA7bAea 1 0 0 Extended eAFjYAAAAAIAAQ== 1.0 eAFzrShJzUtJTfkfkJOYmfcfADQoByQ= input replace 0 1 value 0 input menu 0 0 container width:0 simple 0 0 1 container container 1 1 <AcapellaUserInterface||UI/imageviewmethods.proc> UI::_CIV_CB__ReloadFrames -4 0 1 eAFjAAAAAQAB 1.0 none 0 s Overlap style eAHLyy/KTcz5/48BBAEtqQaD eAHzy89L/e+ckZqcnVqUlJ9YlPI/uKQosyC1+H9wfk5myn8A7wUPLw== 1 0 0 none eAFjYGBgAAAABAAB 1.