LOCUS pTG802_EF1alpha-3xFlag- 9894 bp ds-DNA circular 13-JUL-2021 DEFINITION . KEYWORDS "creator:Gina Dailey - Darzacq/Tjian Lab" "marker:AmpR, PuroR" FEATURES Location/Qualifiers primer complement(108..126) /label="oTG1552_pBS_diffusion_substrate_300bp" /note="sequence: ctctagcttcccggcaaca" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 CDS complement(650..718) /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 promoter complement(719..823) /label="AmpR promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(802..823) /label="oTG1179_FRT_AmpR_5’" /note="sequence: CTATACTTTCTAGAGAATAGGAACTTCcgcggaacccctatttgtttat" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer complement(821..839) /label="oTG1553_pBS_diffusion_substrate_1kb" /note="sequence: ttttcggggaaatgtgcgc" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 rep_origin complement(849..1303) /label="f1 ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer 1441..1460 /label="oTG1336_M13F_extended" /note="sequence: GTTGTAAAACGACGGCCAGT" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer_bind 1443..1460 /label="M13-fwd" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind 1444..1460 /label="M13 fwd" /ApEinfo_revcolor=#a020f0 /ApEinfo_fwdcolor=#a020f0 misc_feature 1590..1602 /label="PB Terminal repeat" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1637..1652 /label="IR (inverted repeat) truncated?" /ApEinfo_revcolor=#339966 /ApEinfo_fwdcolor=#339966 promoter 2071..2570 /label="PGK promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS 2592..3191 /label="PuroR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 polyA_signal 3224..3448 /label="bGH poly(A) signal" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac promoter 3488..3699 /label="EF-1α core promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 3488..3699 /label="EF-1α core promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 LTR 3712..3980 /label="5' LTR (truncated)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 LTR 3712..3980 /label="5' LTR (truncated)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer 3991..4010 /label="oTG1576_3xFlag-Halo_upstream_F" /note="sequence: CCGGCGCCTACTCTAGCCAC" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS 4012..4014 /label="ATG" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 CDS 4012..6951 /label="Translation 4012-6951" CDS 4015..4080 /label="3xFLAG" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer complement(4057..4081) /label="oTG1617_Flag_RT3_R" /note="sequence: CTAGCgccctattgcttgtcatcgtcatccttgtaatc" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4057..4081) /label="oTG1613_Flag_RT1_R" /note="sequence: CTAGCttgctattgcttgtcatcgtcatccttgtaatc" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4057..4081) /label="oTG1615_Flag_RT2_R" /note="sequence: CTAGCttgctagtgcttgtcatcgtcatccttgtaatc" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 4089..4114 /label="oTG945_60xHalo_F" /note="sequence: CCGGCTCCGGCAGCGGTAGCATGGCAGAAATCGGTACTGGCTTTC" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer 4090..4110 /label="oTG778_5'Halo" /note="sequence: ATGGCAGAAATCGGTACTGGC" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer 4090..4110 /label="oTG898_H10_Halo_F" /note="sequence: TTCTCACCATCACCATCACCACCACCACCACCATATGGCAGAAATCGGTACTGGC" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 4090..4110 /label="oTG1573_GSGSKCK_Halo_F" /note="sequence: GGTAGTGGTAGTAAGTGTAAAATGGCAGAAATCGGTACTGGC" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer 4090..4110 /label="oTG1582_SNAPf_linker_Halo_F" /note="sequence: TGGGCAAGCCTGGGCTGGGTggtagtggtaccggttctATGGCAGAAATCGGTACTGGC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS 4090..4980 /label="HaloTag®" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer 4093..4113 /label="oTG1144_inVitro_60xHalo_F" /note="sequence: GGCTCCGGCAGCGGTAGCGCAGAAATCGGTACTGGCTTT" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer 4096..4118 /label="oTG995_Halo_linker_F" /note="sequence: GATCTGGAGGTTCAGGAGGCAGCGGTGGAAGTGAAATCGGTACTGGCTTTCCATT" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer 4096..4118 /label="oTG1280_60mer_linker_Halo_F" /note="sequence: GGAGTGGTAGCGGCAGTATTAAGgaaatcggtactggctttccatt" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer complement(4101..4120) /label="oTG1172_Halo_int_R2" /note="sequence: CGAATGGAAAGCCAGTACCG" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer complement(4106..4125) /label="oTG1214_Halo_int_R_75" /note="sequence: GGGGTCGAATGGAAAGCCAG" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer complement(4129..4148) /label="oTG1096_Halo_int_R" /note="sequence: TCGCCCAGGACTTCCACATA" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer complement(4150..4169) /label="oTG1173_Halo_int_R3" /note="sequence: ACATCGACGTAGTGCATGCG" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer 4311..4332 /label="oTG1513_Halo_qPCR_F1" /note="sequence: ACCAGACCTGGGTTATTTCTTC" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer 4451..4470 /label="oTG1208_Halo_int_F" /note="sequence: AGCGCGTCAAAGGTATTGCA" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer complement(4471..4490) /label="oTG1514_Halo_qPCR_R1" /note="sequence: GGGCGGATGAACTCCATAAA" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer complement(4565..4587) /label="oTG1674_Halo_V167C_R" /note="sequence: GTTCTGATCGATGATCAGCTTGC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4565..4587) /label="oTG1691_Halo_V167C_R" /note="sequence: GTTCTGATCGATGATCAGCTTGC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4574..4599) /label="oTG1676_Halo_G171C_R" /note="sequence: CTCGATAAAAACGTTCTGATCGATGA" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4574..4599) /label="oTG1693_Halo_G171C_R" /note="sequence: CTCGATAAAAACGTTCTGATCGATGA" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 4590..4610 /label="oTG1673_Halo_V167C_F" /note="sequence: tgtTTTATCGAGGGTACGCTGCC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 4590..4610 /label="oTG1690_Halo_V167C_F" /note="sequence: tgtTTTATCGAGGGTACGCTGCC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4592..4611) /label="oTG1678_Halo_M175C_R" /note="sequence: CGGCAGCGTACCCTCGATAA" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4592..4611) /label="oTG1695_Halo_M175C_R" /note="sequence: CGGCAGCGTACCCTCGATAA" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 4601..4620 /label="oTG1675_Halo_G171C_F" /note="sequence: tgtACGCTGCCGATGGGTGTC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 4601..4620 /label="oTG1692_Halo_G171C_F" /note="sequence: tgtACGCTGCCGATGGGTGTC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 4615..4632 /label="oTG1694_Halo_M175C_F" /note="sequence: tgtGGTGTCGTCCGCCCGCTG" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 4615..4632 /label="oTG1677_Halo_M175C_F" /note="sequence: tgtGGTGTCGTCCGCCCGCTG" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4629..4650) /label="oTG1207_Halo_int_R" /note="sequence: GTCCATCTCGACTTCAGTCAGC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 4636..4657 /label="oTG1515_Halo_qPCR_F2" /note="sequence: GAAGTCGAGATGGACCATTACC" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer complement(4758..4777) /label="oTG1516_Halo_qPCR_R2" /note="sequence: GCAGCCAGTCCATGTATTCT" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer 4871..4890 /label="oTG1095_Halo_int_F" /note="sequence: ACTGCAAGGCTGTGGACATC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 4898..4920 /label="oTG979_3'_Halo_seq" /note="sequence: GTCTGAATCTGCTGCAAGAAGAC" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer complement(4948..4965) /label="oTG1312_Halo_tubUTR_R" /note="sequence: cgcgacgcttagtgtgccgcTTACGTCGACAGCCAGCGCGC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer complement(4960..4979) /label="oTG1261_ParB2_Halo_stop_R" /note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(4960..4979) /label="oTG1261_ParB2_Halo_stop_R" /note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer complement(4961..4980) /label="oTG793_3'Halo_R" /note="sequence: GCCGGAAATCTCGAGCGTCG" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer complement(4961..4980) /label="oTG779_Halo-stop_pETSUMO" /note="sequence: GCTCCAATTGGGTACCCATGGAGCCGGAAATCTCGAGCGTCG" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer complement(4961..4982) /label="oTG1583_Halo_noNLS_R" /note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCgccggaaatctcgagcgtcg" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 4972..4991 /label="Gibson overhang" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer 4972..4991 /label="oTG1618_SNAP-3xNLS_gBlock_F" /note="sequence: ATTTCCGGCggtagtggtac" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a misc_feature 4999..5544 /label="SNAPf" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 CDS 4999..5544 /label="Translation 4999-5544" primer 5550..5569 /label="oTG1683_3xNLS-P2A-T2A_KpnI_F" /note="sequence: atttccggcggtagtggtaccTCAGGAAGTGGAGATGGCG" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 5577..5626 /label="oTG1620_HaloSNAP60mer-T2AP2A-SNAP_F" /note="sequence: GATCAATGGACGGGCAGATgctagcATGAAAATGGAAGAGCTGTTCAAGA" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 5601..5626 /label="oTG909_60mer_pcDNA_F" /note="sequence: CCACTAGTCCAGTGTGGTGGCCACCATGAAAATGGAAGAGCTGTTCAAGA" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer 5601..5626 /label="oTG1622_HaloSNAP3xN-TP2A-60merSNAP_F" /note="sequence: gaaaatcccggcccaGgtaccATGAAAATGGAAGAGCTGTTCAAGA" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a misc_feature 5602..6216 /label="60mer" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer 5605..5626 /label="oTG936_60xGFP_F" /note="sequence: AAAATGGAAGAGCTGTTCAAGA" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 5605..5630 /label="oTG977_Linker_ctGFP_F" /note="sequence: GTTCAGGAGGCAGCGGTGGAAGTAAAATGGAAGAGCTGTTCAAGAAACA" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer complement(6198..6216) /label="oTG1621_HaloSNAP60mer-T2AP2A-SNAP_R" /note="sequence: ttgtggcgccgctgcccatatgTTCGGTGCAGCCACGAATC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(6198..6216) /label="oTG1623_HaloSNAP60mer-TP2A-SNAP_R" /note="sequence: tccacttccgccaccggtaccTTCGGTGCAGCCACGAATC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(6198..6218) /label="oTG1623_HaloSNAP60mer-TP2A-SNAP_R" /note="sequence: tccacttccgccaccggtaccTTCGGTGCAGCCACGAATC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(6198..6238) /label="oTG1621_HaloSNAP60mer-T2AP2A-SNAP_R" /note="sequence: ttgtggcgccgctgcccatatgTTCGGTGCAGCCACGAATC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 6216..6234 /label="oTG1725_SNAPf-3xNLS_P2A_F" /note="sequence: AGAAAGGTAGGATCCACCGGAcatatgggcagcggcgcc" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 6223..6240 /label="oTG1684_P2A-T2A_KpnI_F" /note="sequence: atttccggcggtagtggtaccGGCAGCGGCGCCACAAAC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a misc_feature 6223..6288 /label="P2A" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 6307..6369 /label="T2A" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer complement(6352..6378) /label="oTG1685_P2A-T2A_KpnI_R" /note="sequence: tttgtccatagaaccggtaccTGGGCCGGGATTTTCCTC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(6358..6375) /label="oTG1726_T2A-KpnI_R" /note="sequence: ggtacctgggccgggatt" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(6398..6420) /label="oTG1619_SNAP-3xNLS_gBlock_R" /note="sequence: gcagtctttgtccatagaacctg" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a misc_feature 6400..6420 /label="Gibson overhang" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer 6400..6427 /label="oTG1577_Halo_linker_SNAPf_F" /note="sequence: CGACGCTCGAGATTTCCGGCggtagtggtaccggttctATGGACAAAGACTGCGAAATGA" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer 6406..6428 /label="oTG1562_FKBP-SNAPf_F2" /note="sequence: atggacaaagactgcgaaatgaa" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer 6406..6428 /label="oTG1580_Kozak_SNAPf_F" /note="sequence: CCGGCGCCTACTCTAGCCACCatggacaaagactgcgaaatgaa" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 CDS 6406..6951 /label="SNAPf" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd gene 6406..6951 /label="SNAP26f" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS 6406..6951 /label="Translation 6406-6951" source 6406..6951 /label="source" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(6493..6513) /label="oTG1722_SNAPf_v2_int_R" /note="sequence: tttgcccaggaagatgatacg" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(6932..6951) /label="oTG1581_SNAPf_3’_R" /note="sequence: acccagcccaggcttgccca" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(6932..6951) /label="oTG1563_FKBP-SNAPf_R2" /note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer complement(6932..6952) /label="oTG1563_FKBP-SNAPf_R2" /note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer complement(6932..6952) /label="oTG1578_SNAPf_noNLS_R" /note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCacccagcccaggcttgccca" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer complement(6932..6971) /label="oTG1563_FKBP-SNAPf_R2" /note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer complement(6932..6985) /label="oTG1578_SNAPf_noNLS_R" /note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCacccagcccaggcttgccca" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer 6952..6974 /label="oTG967_pHalo-Stop_F" /note="sequence: TAGGATCCACCGGATCTAGATAACT" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 CDS 6952..6976 /label="Translation 6952-6976" primer complement(6957..6985) /label="oTG1579_3xNLS_downstream_R" /note="sequence: TGATTATGATCAGTTATCTAGATCCGGTG" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 6966..6976 /label="stop codons" /ApEinfo_revcolor=#ff0000 /ApEinfo_fwdcolor=#ff0000 misc_feature 7053..7053 /label="add_before" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS 7053..7070 /label="MCS" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc polyA_signal 7186..7307 /label="SV40 poly(A) signal" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer complement(7279..7307) /label="oTG885_SV40_pA_F" /note="sequence: TAAGATACATTGATGAGTTTGGACAAACC" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature complement(8067..8085) /label="IR (inverted repeat)" /ApEinfo_revcolor=#339966 /ApEinfo_fwdcolor=#339966 misc_feature complement(8089..8101) /label="PB Terminal repeat" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind complement(8592..8608) /label="M13 rev" /ApEinfo_revcolor=#a020f0 /ApEinfo_fwdcolor=#a020f0 protein_bind 8616..8632 /label="lac operator" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b promoter complement(8640..8646) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter complement(8647..8664) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(8658..8679) /label="oTG980_Vector-Fwd-GA" /note="sequence: ccggatgacagtgacgaggagaactgaACCCCAGGCTTTacacTTTATG" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e promoter complement(8665..8670) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 protein_bind 8685..8706 /label="CAP binding site" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b primer 8737..8757 /label="oTG1156_mCherry-LacI-Rev" /note="sequence: AAGCTTCCATTCGGGAAACCTGTCGtgCCAG" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer 8757..8777 /label="oTG1089_Rev-LacI-NLS-NOSTOP-GA" /note="sequence: AACCTTCCTCTTCTTCTTAGGAGGCTGCATTAATGAATCGGCCA" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer 8757..8778 /label="oTG1084_Rev-LacI-GA" /note="sequence: TCAAACCTTCCTCTtcTTcTTaGgagGCTGCATTAATGAATCGGCCAA" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer 8814..8831 /label="oTG775_pET_BbvCI_insert_seq" /note="sequence: TTCCGCTTCCTCGCTCAC" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 rep_origin complement(8994..9581) /label="ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer 9635..9658 /label="oTG1180_AmpR_seq" /note="sequence: ACGTTAAGGGATTTTGGTCATGAG" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(9721..9751) /label="oTG1177_FRT_AmpR_3’flanking" /note="sequence: CTATACTTTCTAGAGAATAGGAACTTCctgtcagaccaagtttactcatatatacttt" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer complement(9721..9751) /label="oTG1554_pBS_diffusion_substrate_3kb" /note="sequence: ctgtcagaccaagtttactcatatatacttt" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer 9722..9751 /label="oTG1182_E75_AmpR_F" /note="sequence: gactaactgccactcgcagcgcgcagatcgAAGTATATATGAGTAAACTTGGTCTGACAG" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer 9751..9775 /label="oTG1549_pBS_849_adapter" /note="sequence: GCTGTGTCTTATTACACACAGTCGttaccaatgcttaatcagtgaggc" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 CDS complement(join(9752..9894,1..649)) /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 9752..9775 /label="oTG1176_FRT_AmpR_3’" /note="sequence: CTATTCTCTAGAAAGTATAGGAACTTCttaccaatgcttaatcagtgaggc" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer complement(9798..9820) /label="oTG1550_pBS_diffusion_substrate_100bp" /note="sequence: caggcaactatggatgaacgaaa" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 ORIGIN 1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT 901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA 961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT 1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA 1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA 1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG 1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA 1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG 1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG 1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC 1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG 1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG 1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG 1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT 1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA 1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT 1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT 1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT 1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA 1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC 2041 TGGGGGGATA CGGGGAAAAG GCGAACGCGT gggtagggga ggcgcttttc ccaaggcagt 2101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc 2161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct 2221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt 2281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca 2341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct 2401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc 2461 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa 2521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc 2581 caAGCTTCAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC 2641 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG 2701 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG 2761 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA 2821 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG 2881 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC 2941 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA 3001 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC 3061 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA 3121 CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC 3181 CCGGTGCCTG AGCGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC 3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC 3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT 3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG 3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AACGCGATCG CTCCGGTGCC 3481 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC 3541 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC 3601 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG 3661 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC 3721 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC 3781 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA 3841 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC 3901 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT 3961 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCCAC CATGGACTAC 4021 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGACAAG 4081 CTTGGCTCCA TGGCAGAAAT CGGTACTGGC TTTCCATTCG ACCCCCATTA TGTGGAAGTC 4141 CTGGGCGAGC GCATGCACTA CGTCGATGTT GGTCCGCGCG ATGGCACCCC TGTGCTGTTC 4201 CTGCACGGTA ACCCGACCTC CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG 4261 ACCCATCGCT GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA ACCAGACCTG 4321 GGTTATTTCT TCGACGACCA CGTCCGCTTC ATGGATGCCT TCATCGAAGC CCTGGGTCTG 4381 GAAGAGGTCG TCCTGGTCAT TCACGACTGG GGCTCCGCTC TGGGTTTCCA CTGGGCCAAG 4441 CGCAATCCAG AGCGCGTCAA AGGTATTGCA TTTATGGAGT TCATCCGCCC TATCCCGACC 4501 TGGGACGAAT GGCCAGAATT TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC 4561 GGCCGCAAGC TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC GATGGGTGTC 4621 GTCCGCCCGC TGACTGAAGT CGAGATGGAC CATTACCGCG AGCCGTTCCT GAATCCTGTT 4681 GACCGCGAGC CACTGTGGCG CTTCCCAAAC GAGCTGCCAA TCGCCGGTGA GCCAGCGAAC 4741 ATCGTCGCGC TGGTCGAAGA ATACATGGAC TGGCTGCACC AGTCCCCTGT CCCGAAGCTG 4801 CTGTTCTGGG GCACCCCAGG CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA 4861 AGCCTGCCTA ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT GCAAGAAGAC 4921 AACCCGGACC TGATCGGCAG CGAGATCGCG CGCTGGCTGT CGACGCTCGA GATTTCCGGC 4981 ggtagtggta caggttctAT GGATAAAGAC TGTGAGATGA AGCGGACAAC CCTTGATTCC 5041 CCTCTGGGGA AACTGGAGCT CAGTGGGTGC GAACAGGGGC TGCACAGAAT TATATTTCTT 5101 GGGAAGGGCA CGTCTGCTGC AGATGCTGTC GAGGTCCCAG CCCCGGCTGC CGTGCTTGGT 5161 GGACCAGAGC CTCTCATGCA AGCTACTGCT TGGCTGAACG CGTATTTCCA CCAACCTGAA 5221 GCGATAGAAG AGTTCCCTGT TCCTGCTCTG CACCACCCGG TTTTCCAACA GGAATCTTTC 5281 ACGAGACAAG TATTGTGGAA ACTTCTCAAA GTGGTCAAAT TCGGTGAAGT AATATCCTAT 5341 TCACACCTGG CAGCTCTCGC CGGGAACCCG GCGGCAACCG CTGCGGTTAA AACTGCACTC 5401 TCTGGTAATC CTGTACCAAT ACTTATACCG TGCCACAGGG TTGTCCAAGG CGACTTGGAC 5461 GTTGGAGGGT ATGAAGGGGG CCTCGCTGTG AAAGAGTGGC TTCTCGCGCA CGAAGGTCAT 5521 CGGCTCGGTA AGCCAGGGCT CGGGGAAATC TCAGGAAGTG GAGATGGCGC CGGATTGATC 5581 AATGGACGGG CAGATgctag cATGAAAATG GAAGAGCTGT TCAAGAAACA CAAGATCGTT 5641 GCCGTGCTGC GTGCCAATAG TGTGGAAGAA GCGAAAAAGA AAGCGCTGGC GGTTTTCCTG 5701 GGCGGCGTTC ATCTGATTGA AATTACCTTT ACCGTGCCGG ATGCGGATAC CGTGATTAAG 5761 GAACTGAGCT TTCTGAAGGA AATGGGCGCG ATTATTGGTG CGGGCACCGT GACCAGCGTG 5821 GAGCAGTGCC GTAAAGCGGT GGAAAGTGGC GCCGAATTCA TTGTGAGTCC GCACCTGGAC 5881 GAGGAAATTA GCCAATTTTG CAAGGAGAAG GGTGTGTTCT ATATGCCAGG CGTTATGACC 5941 CCGACCGAAC TGGTGAAAGC CATGAAACTG GGCCATACCA TCTTAAAACT GTTTCCGGGT 6001 GAGGTGGTGG GTCCGCAGTT TGTTAAAGCG ATGAAAGGTC CGTTTCCGAA TGTGAAATTT 6061 GTGCCAACCG GCGGTGTTAA TCTGGACAAT GTGTGCGAAT GGTTCAAAGC GGGCGTGCTG 6121 GCCGTGGGCG TGGGCAGCGC GTTAGTGAAA GGCACCCCGG TGGAAGTGGC GGAAAAGGCC 6181 AAGGCGTTCG TTGAGAAGAT TCGTGGCTGC ACCGAAcata tgggcagcgg cgccacaaac 6241 ttctctctgc taaagcaagc aggtgatgtt gaagaaaacc ccgggcctAC TAGTGCTGGA 6301 AAGCTTggct ccggcgaggg caggggaagt cttctaacat gcggggacgt ggaggaaaat 6361 cccggcccaG gtaccggtgg cggaagtgga ggtggttcag gttctatgga caaagactgc 6421 gaaatgaagc gcaccaccct ggatagccct ctgggcaagc tggaactgtc tgggtgcgaa 6481 cagggcctgc accgtatcat cttcctgggc aaaggaacat ctgccgccga cgccgtggaa 6541 gtgcctgccc cagccgccgt gctgggcgga ccagagccac tgatgcaggc caccgcctgg 6601 ctcaacgcct actttcacca gcctgaggcc atcgaggagt tccctgtgcc agccctgcac 6661 cacccagtgt tccagcagga gagctttacc cgccaggtgc tgtggaaact gctgaaagtg 6721 gtgaagttcg gagaggtcat cagctacagc cacctggccg ccctggccgg caatcccgcc 6781 gccaccgccg ccgtgaaaac cgccctgagc ggaaatcccg tgcccattct gatcccctgc 6841 caccgggtgg tgcagggcga cctggacgtg gggggctacg agggcgggct cgccgtgaaa 6901 gagtggctgc tggcccacga gggccacaga ctgggcaagc ctgggctggg tGGATCCACC 6961 GGATCTAGAT AACTGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA 7021 AAAAACCTCC CACACCTCCC CCTGAACCTG AACGCGGCCG CtggGTCGAC AATCAACCTC 7081 TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC 7141 TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCGTT AACTAAACTT GTTTATTGCA 7201 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT 7261 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGAAT 7321 TGACTCAAAT GATGTCAATT AGTCTATCAG AAGCTCATCT GGTCTCCCTT CCGGGGGACA 7381 AGACATCCCT GTTTAATATT TAAACAGCAG TGTTCCCAAA CTGGGTTCTT ATATCCCTTG 7441 CTCTGGTCAA CCAGGTTGCA GGGTTTCCTG TCCTCACAGG AACGAAGTCC CTAAAGAAAC 7501 AGTGGCAGCC AGGTTTAGCC CCGGAATTGA CTGGATTCCT TTTTTAGGGC CCATTGGTAT 7561 GGCTTTTTCC CCGTATCCCC CCAGGTGTCT GCAGGCTCAA AGAGCAGCGA GAAGCGTTCA 7621 GAGGAAAGCG ATCCCGTGCC ACCTTCCCCG TGCCCGGGCT GTCCCCGCAC GCTGCCGGCT 7681 CGGGGATGCG GGGGGAGCGC CGGACCGGAG CGGAGCCCCG GGCGGCTCGC TGCTGCCCCC 7741 TAGCGGGGGA GGGACGTAAT TACATCCCTG GGGGCTTTGG GGGGGGGCTG TCCCTGATAT 7801 CTATAACAAG AAATATATAT ATAATAAGTT ATCACGTAAG TAGAACATGA AATAACAATA 7861 TAATTATCGT ATGAGTTAAT CTTAAAGTCA CGTAAAAGAT AATCATGCGT CATTTTGACT 7921 CACGCGGTCG TTATAGTTCA AAATCAGTGA CACTTACCGC ATTGACAAGC ACGCCTCACG 7981 GGAGCTCCAA GCGGCGACTG AGATGTCCTA AATGCACAGC GACGGATTCG CGCTATTTAG 8041 AAAGAGAGAG CAATATTTCA AGAATGCATG CGTCAATTTT ACGCAGACTA TCTTTCTAGG 8101 GTTAATCTAG CTGCATCAGG ATCATATCGT CGGGTCTTTT TTCCGGCTCA GTCATCGCCC 8161 AAGCTGGCGC TATCTGGGCA TCGGGGAGGA AGAAGCCCGT GCCTTTTCCC GCGAGGTTGA 8221 AGCGGCATGG AAAGAGTTTG CCGAGGATGA CTGCTGCTGC ATTGACGTTG AGCGAAAACG 8281 CACGTTTACC ATGATGATTC GGGAAGGTGT GGCCATGCAC GCCTTTAACG GTGAACTGTT 8341 CGTTCAGGCC ACCTGGGATA CCAGTTCGTC GCGGCTTTTC CGGACACAGT TCCGGATGGT 8401 CAGCCCGAAG CGCATCAGCA ACCCGAACAA TACCGGCGAC AGCCGGAACT GCCGTGCCGG 8461 TGTGCAGATT AATGACAGCG GTGCGGCGCT GGGATATTAC GTCAGCGAGG ACGGGTATCC 8521 TGGCTGGATG CCGCAGAAAT GGACATGGAT ACCCCGTGAG TTACCCGGCG GGCGCGCTTG 8581 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC 8641 AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC 8701 ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG 8761 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT 8821 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC 8881 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 8941 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 9001 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 9061 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 9121 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 9181 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 9241 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 9301 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 9361 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 9421 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 9481 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 9541 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 9601 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 9661 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 9721 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 9781 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 9841 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAG //