#this is a .ijm imageJ macros to conver RGB to 8bit image (tiff) input = getDirectory("/Users/ranjith.papareddy/Downloads/seed_size/onlytiffs"); output = getDirectory("/Users/ranjith.papareddy/Downloads/seed_size/onlytiffs/8bit_converted"); suffix = ".tif"; //you only want to apply to tiff images, no need to ask path = getDirectory("image"); processFolder(input); function processFolder(input) { list = getFileList(input); for (i = 0; i < list.length; i++) { if(File.isDirectory(input + list[i])) //if it's a directory, go to subfolder processFolder("" + input + list[i]); if(endsWith(list[i], suffix)) //if it's a tiff image, process it processFile(input, output, list[i]); //if it's neither a tiff nor a directory, do nothing } } function processFile(input, output, file) { print("Processing: " + input + file); open(input + file); run("8-bit"); saveAs("tiff", output + file ); print("Saved to: " + output); close(); }