ptg800-ef1alpha-3xflag-halo-snapf-3xnls-t2a-p2a-h2b-snapf-3xnls.gb
LOCUS pTG800_EF1alpha-3xFlag- 9864 bp ds-DNA circular 20-APR-2021
DEFINITION .
KEYWORDS "creator:Gina Dailey - Darzacq/Tjian Lab" "marker:AmpR, PuroR"
FEATURES Location/Qualifiers
primer complement(108..126)
/label="oTG1552_pBS_diffusion_substrate_300bp"
/note="sequence: ctctagcttcccggcaaca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS complement(650..718)
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter complement(719..823)
/label="AmpR promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(802..823)
/label="oTG1179_FRT_AmpR_5’"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCcgcggaacccctatttgtttat"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(821..839)
/label="oTG1553_pBS_diffusion_substrate_1kb"
/note="sequence: ttttcggggaaatgtgcgc"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
rep_origin complement(849..1303)
/label="f1 ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 1441..1460
/label="oTG1336_M13F_extended"
/note="sequence: GTTGTAAAACGACGGCCAGT"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind 1443..1460
/label="M13-fwd"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 1444..1460
/label="M13 fwd"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
misc_feature 1590..1602
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 1637..1652
/label="IR (inverted repeat) truncated?"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
promoter 2071..2570
/label="PGK promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS 2592..3191
/label="PuroR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
polyA_signal 3224..3448
/label="bGH poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 3991..4010
/label="oTG1576_3xFlag-Halo_upstream_F"
/note="sequence: CCGGCGCCTACTCTAGCCAC"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS 4012..4014
/label="ATG"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 4012..6801
/label="Translation 4012-6801"
CDS 4015..4080
/label="3xFLAG"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer complement(4057..4081)
/label="oTG1613_Flag_RT1_R"
/note="sequence: CTAGCttgctattgcttgtcatcgtcatccttgtaatc"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4057..4081)
/label="oTG1617_Flag_RT3_R"
/note="sequence: CTAGCgccctattgcttgtcatcgtcatccttgtaatc"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4057..4081)
/label="oTG1615_Flag_RT2_R"
/note="sequence: CTAGCttgctagtgcttgtcatcgtcatccttgtaatc"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4089..4114
/label="oTG945_60xHalo_F"
/note="sequence: CCGGCTCCGGCAGCGGTAGCATGGCAGAAATCGGTACTGGCTTTC"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 4090..4110
/label="oTG898_H10_Halo_F"
/note="sequence: TTCTCACCATCACCATCACCACCACCACCACCATATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 4090..4110
/label="oTG1582_SNAPf_linker_Halo_F"
/note="sequence: TGGGCAAGCCTGGGCTGGGTggtagtggtaccggttctATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 4090..4110
/label="oTG1573_GSGSKCK_Halo_F"
/note="sequence: GGTAGTGGTAGTAAGTGTAAAATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer 4090..4110
/label="oTG778_5'Halo"
/note="sequence: ATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
CDS 4090..4980
/label="HaloTag®"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 4093..4113
/label="oTG1144_inVitro_60xHalo_F"
/note="sequence: GGCTCCGGCAGCGGTAGCGCAGAAATCGGTACTGGCTTT"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer 4096..4118
/label="oTG995_Halo_linker_F"
/note="sequence: GATCTGGAGGTTCAGGAGGCAGCGGTGGAAGTGAAATCGGTACTGGCTTTCCATT"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer 4096..4118
/label="oTG1280_60mer_linker_Halo_F"
/note="sequence: GGAGTGGTAGCGGCAGTATTAAGgaaatcggtactggctttccatt"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(4101..4120)
/label="oTG1172_Halo_int_R2"
/note="sequence: CGAATGGAAAGCCAGTACCG"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(4106..4125)
/label="oTG1214_Halo_int_R_75"
/note="sequence: GGGGTCGAATGGAAAGCCAG"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(4129..4148)
/label="oTG1096_Halo_int_R"
/note="sequence: TCGCCCAGGACTTCCACATA"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(4150..4169)
/label="oTG1173_Halo_int_R3"
/note="sequence: ACATCGACGTAGTGCATGCG"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 4311..4332
/label="oTG1513_Halo_qPCR_F1"
/note="sequence: ACCAGACCTGGGTTATTTCTTC"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer 4451..4470
/label="oTG1208_Halo_int_F"
/note="sequence: AGCGCGTCAAAGGTATTGCA"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(4471..4490)
/label="oTG1514_Halo_qPCR_R1"
/note="sequence: GGGCGGATGAACTCCATAAA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(4565..4587)
/label="oTG1691_Halo_V167C_R"
/note="sequence: GTTCTGATCGATGATCAGCTTGC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4565..4587)
/label="oTG1674_Halo_V167C_R"
/note="sequence: GTTCTGATCGATGATCAGCTTGC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4574..4599)
/label="oTG1676_Halo_G171C_R"
/note="sequence: CTCGATAAAAACGTTCTGATCGATGA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4574..4599)
/label="oTG1693_Halo_G171C_R"
/note="sequence: CTCGATAAAAACGTTCTGATCGATGA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4590..4610
/label="oTG1673_Halo_V167C_F"
/note="sequence: tgtTTTATCGAGGGTACGCTGCC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4590..4610
/label="oTG1690_Halo_V167C_F"
/note="sequence: tgtTTTATCGAGGGTACGCTGCC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4592..4611)
/label="oTG1678_Halo_M175C_R"
/note="sequence: CGGCAGCGTACCCTCGATAA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4592..4611)
/label="oTG1695_Halo_M175C_R"
/note="sequence: CGGCAGCGTACCCTCGATAA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4601..4620
/label="oTG1692_Halo_G171C_F"
/note="sequence: tgtACGCTGCCGATGGGTGTC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4601..4620
/label="oTG1675_Halo_G171C_F"
/note="sequence: tgtACGCTGCCGATGGGTGTC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4615..4632
/label="oTG1694_Halo_M175C_F"
/note="sequence: tgtGGTGTCGTCCGCCCGCTG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4615..4632
/label="oTG1677_Halo_M175C_F"
/note="sequence: tgtGGTGTCGTCCGCCCGCTG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4629..4650)
/label="oTG1207_Halo_int_R"
/note="sequence: GTCCATCTCGACTTCAGTCAGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 4636..4657
/label="oTG1515_Halo_qPCR_F2"
/note="sequence: GAAGTCGAGATGGACCATTACC"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer complement(4758..4777)
/label="oTG1516_Halo_qPCR_R2"
/note="sequence: GCAGCCAGTCCATGTATTCT"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 4871..4890
/label="oTG1095_Halo_int_F"
/note="sequence: ACTGCAAGGCTGTGGACATC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 4898..4920
/label="oTG979_3'_Halo_seq"
/note="sequence: GTCTGAATCTGCTGCAAGAAGAC"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer complement(4948..4965)
/label="oTG1312_Halo_tubUTR_R"
/note="sequence: cgcgacgcttagtgtgccgcTTACGTCGACAGCCAGCGCGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(4960..4979)
/label="oTG1261_ParB2_Halo_stop_R"
/note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(4960..4979)
/label="oTG1261_ParB2_Halo_stop_R"
/note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(4961..4980)
/label="oTG779_Halo-stop_pETSUMO"
/note="sequence: GCTCCAATTGGGTACCCATGGAGCCGGAAATCTCGAGCGTCG"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer complement(4961..4980)
/label="oTG793_3'Halo_R"
/note="sequence: GCCGGAAATCTCGAGCGTCG"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer complement(4961..4982)
/label="oTG1583_Halo_noNLS_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCgccggaaatctcgagcgtcg"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 4972..4991
/label="Gibson overhang"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 4972..4991
/label="oTG1618_SNAP-3xNLS_gBlock_F"
/note="sequence: ATTTCCGGCggtagtggtac"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 4999..5544
/label="SNAPf"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
CDS 4999..5544
/label="Translation 4999-5544"
primer 5550..5569
/label="oTG1683_3xNLS-P2A-T2A_KpnI_F"
/note="sequence: atttccggcggtagtggtaccTCAGGAAGTGGAGATGGCG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 5602..5670
/label="3xNLS"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 5677..5694
/label="oTG1684_P2A-T2A_KpnI_F"
/note="sequence: atttccggcggtagtggtaccGGCAGCGGCGCCACAAAC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 5677..5742
/label="P2A"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 5761..5823
/label="T2A"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
primer complement(5806..5832)
/label="oTG1685_P2A-T2A_KpnI_R"
/note="sequence: tttgtccatagaaccggtaccTGGGCCGGGATTTTCCTC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
CDS 5830..6207
/label="H2be"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 5830..6207
/label="Translation 5830-6207"
primer complement(6248..6270)
/label="oTG1619_SNAP-3xNLS_gBlock_R"
/note="sequence: gcagtctttgtccatagaacctg"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 6250..6270
/label="Gibson overhang"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 6250..6277
/label="oTG1577_Halo_linker_SNAPf_F"
/note="sequence: CGACGCTCGAGATTTCCGGCggtagtggtaccggttctATGGACAAAGACTGCGAAATGA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 6256..6278
/label="oTG1562_FKBP-SNAPf_F2"
/note="sequence: atggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 6256..6278
/label="oTG1580_Kozak_SNAPf_F"
/note="sequence: CCGGCGCCTACTCTAGCCACCatggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
CDS 6256..6801
/label="SNAPf"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
gene 6256..6801
/label="SNAP26f"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 6256..6801
/label="Translation 6256-6801"
source 6256..6801
/label="source"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(6782..6801)
/label="oTG1581_SNAPf_3’_R"
/note="sequence: acccagcccaggcttgccca"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(6782..6801)
/label="oTG1563_FKBP-SNAPf_R2"
/note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(6782..6802)
/label="oTG1578_SNAPf_noNLS_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCacccagcccaggcttgccca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(6782..6821)
/label="oTG1563_FKBP-SNAPf_R2"
/note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
CDS 6802..6813
/label="HaloTag®"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
CDS 6802..6942
/label="Translation 6802-6942"
primer 6817..6837
/label="oTG1415_7-SspB-p65-Fwd-OptoFly-GA"
/note="sequence: ggagatggcgctggactgatc"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
CDS 6817..6840
/label="GDGAGLIN linker"
/ApEinfo_revcolor=#cd4489
/ApEinfo_fwdcolor=#cd4489
misc_feature 6843..6843
/label="add_after"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 6853..6873
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 6877..6897
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 6901..6921
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
primer 6920..6944
/label="oTG967_pHalo-Stop_F"
/note="sequence: TAGGATCCACCGGATCTAGATAACT"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(6927..6955)
/label="oTG1579_3xNLS_downstream_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTG"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 6936..6946
/label="stop codons"
/ApEinfo_revcolor=#ff0000
/ApEinfo_fwdcolor=#ff0000
misc_feature 7023..7023
/label="add_before"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 7023..7040
/label="MCS"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
polyA_signal 7156..7277
/label="SV40 poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(7249..7277)
/label="oTG885_SV40_pA_F"
/note="sequence: TAAGATACATTGATGAGTTTGGACAAACC"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature complement(8037..8055)
/label="IR (inverted repeat)"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
misc_feature complement(8059..8071)
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind complement(8562..8578)
/label="M13 rev"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
protein_bind 8586..8602
/label="lac operator"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
promoter complement(8610..8616)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(8617..8634)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(8628..8649)
/label="oTG980_Vector-Fwd-GA"
/note="sequence: ccggatgacagtgacgaggagaactgaACCCCAGGCTTTacacTTTATG"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
promoter complement(8635..8640)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
protein_bind 8655..8676
/label="CAP binding site"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
primer 8707..8727
/label="oTG1156_mCherry-LacI-Rev"
/note="sequence: AAGCTTCCATTCGGGAAACCTGTCGtgCCAG"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 8727..8747
/label="oTG1089_Rev-LacI-NLS-NOSTOP-GA"
/note="sequence: AACCTTCCTCTTCTTCTTAGGAGGCTGCATTAATGAATCGGCCA"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 8727..8748
/label="oTG1084_Rev-LacI-GA"
/note="sequence: TCAAACCTTCCTCTtcTTcTTaGgagGCTGCATTAATGAATCGGCCAA"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 8784..8801
/label="oTG775_pET_BbvCI_insert_seq"
/note="sequence: TTCCGCTTCCTCGCTCAC"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
rep_origin complement(8964..9551)
/label="ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 9605..9628
/label="oTG1180_AmpR_seq"
/note="sequence: ACGTTAAGGGATTTTGGTCATGAG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(9691..9721)
/label="oTG1554_pBS_diffusion_substrate_3kb"
/note="sequence: ctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer complement(9691..9721)
/label="oTG1177_FRT_AmpR_3’flanking"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer 9692..9721
/label="oTG1182_E75_AmpR_F"
/note="sequence: gactaactgccactcgcagcgcgcagatcgAAGTATATATGAGTAAACTTGGTCTGACAG"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer 9721..9745
/label="oTG1549_pBS_849_adapter"
/note="sequence: GCTGTGTCTTATTACACACAGTCGttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
CDS complement(join(9722..9864,1..649))
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 9722..9745
/label="oTG1176_FRT_AmpR_3’"
/note="sequence: CTATTCTCTAGAAAGTATAGGAACTTCttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(9768..9790)
/label="oTG1550_pBS_diffusion_substrate_100bp"
/note="sequence: caggcaactatggatgaacgaaa"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC
61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG
121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA
181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA
241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA
301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA
361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA
421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG
481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG
541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG
601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG
661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC
721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT
901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA
961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA
1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA
1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG
1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA
1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG
1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG
1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC
1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG
1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG
1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG
1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT
1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA
1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT
1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT
1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT
1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA
1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC
2041 TGGGGGGATA CGGGGAAAAG GCGAACGCGT gggtagggga ggcgcttttc ccaaggcagt
2101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
2161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
2221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
2281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
2341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
2401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
2461 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
2521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
2581 caAGCTTCAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC
2641 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG
2701 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG
2761 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA
2821 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG
2881 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC
2941 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA
3001 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC
3061 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA
3121 CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC
3181 CCGGTGCCTG AGCGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AACGCGATCG CTCCGGTGCC
3481 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC
3541 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC
3601 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG
3661 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC
3721 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC
3781 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA
3841 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC
3901 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT
3961 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCCAC CATGGACTAC
4021 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGACAAG
4081 CTTGGCTCCA TGGCAGAAAT CGGTACTGGC TTTCCATTCG ACCCCCATTA TGTGGAAGTC
4141 CTGGGCGAGC GCATGCACTA CGTCGATGTT GGTCCGCGCG ATGGCACCCC TGTGCTGTTC
4201 CTGCACGGTA ACCCGACCTC CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG
4261 ACCCATCGCT GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA ACCAGACCTG
4321 GGTTATTTCT TCGACGACCA CGTCCGCTTC ATGGATGCCT TCATCGAAGC CCTGGGTCTG
4381 GAAGAGGTCG TCCTGGTCAT TCACGACTGG GGCTCCGCTC TGGGTTTCCA CTGGGCCAAG
4441 CGCAATCCAG AGCGCGTCAA AGGTATTGCA TTTATGGAGT TCATCCGCCC TATCCCGACC
4501 TGGGACGAAT GGCCAGAATT TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC
4561 GGCCGCAAGC TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC GATGGGTGTC
4621 GTCCGCCCGC TGACTGAAGT CGAGATGGAC CATTACCGCG AGCCGTTCCT GAATCCTGTT
4681 GACCGCGAGC CACTGTGGCG CTTCCCAAAC GAGCTGCCAA TCGCCGGTGA GCCAGCGAAC
4741 ATCGTCGCGC TGGTCGAAGA ATACATGGAC TGGCTGCACC AGTCCCCTGT CCCGAAGCTG
4801 CTGTTCTGGG GCACCCCAGG CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA
4861 AGCCTGCCTA ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT GCAAGAAGAC
4921 AACCCGGACC TGATCGGCAG CGAGATCGCG CGCTGGCTGT CGACGCTCGA GATTTCCGGC
4981 ggtagtggta caggttctAT GGATAAAGAC TGTGAGATGA AGCGGACAAC CCTTGATTCC
5041 CCTCTGGGGA AACTGGAGCT CAGTGGGTGC GAACAGGGGC TGCACAGAAT TATATTTCTT
5101 GGGAAGGGCA CGTCTGCTGC AGATGCTGTC GAGGTCCCAG CCCCGGCTGC CGTGCTTGGT
5161 GGACCAGAGC CTCTCATGCA AGCTACTGCT TGGCTGAACG CGTATTTCCA CCAACCTGAA
5221 GCGATAGAAG AGTTCCCTGT TCCTGCTCTG CACCACCCGG TTTTCCAACA GGAATCTTTC
5281 ACGAGACAAG TATTGTGGAA ACTTCTCAAA GTGGTCAAAT TCGGTGAAGT AATATCCTAT
5341 TCACACCTGG CAGCTCTCGC CGGGAACCCG GCGGCAACCG CTGCGGTTAA AACTGCACTC
5401 TCTGGTAATC CTGTACCAAT ACTTATACCG TGCCACAGGG TTGTCCAAGG CGACTTGGAC
5461 GTTGGAGGGT ATGAAGGGGG CCTCGCTGTG AAAGAGTGGC TTCTCGCGCA CGAAGGTCAT
5521 CGGCTCGGTA AGCCAGGGCT CGGGGAAATC TCAGGAAGTG GAGATGGCGC CGGATTGATC
5581 AATGGACGGG CAGATgctag cCCGAAGAAA AAGCGGAAGG TCGATCCCAA AAAGAAGAGG
5641 AAAGTGGACC CAAAGAAGAA ACGGAAGGTA catatgggca gcggcgccac aaacttctct
5701 ctgctaaagc aagcaggtga tgttgaagaa aaccccgggc ctACTAGTGC TGGAAAGCTT
5761 ggctccggcg agggcagggg aagtcttcta acatgcgggg acgtggagga aaatcccggc
5821 ccaGgtaccA TGCCTGAGCC TGCGAAGTCC GCTCCCGCCC CGAAGAAGGG CTCCAAGAAG
5881 GCCGTCACCA AGGCCCAGAA GAAGGACGGC AAGAAGCGCA AGCGCAGCCG CAAGGAGAGC
5941 TACTCGGTGT ACGTGTACAA GGTGCTGAAA CAAGTGCACC CCGACACGGG CATCTCCTCC
6001 AAGGCCATGG GCATCATGAA CTCGTTCGTG AACGACATCT TCGAGCGCAT CGCGGGCGAG
6061 GCGTCCCGCC TGGCGCATTA CAACAAGCGC TCGACCATCA CGTCCCGGGA GATCCAGACG
6121 GCCGTGCGCC TGCTGCTGCC CGGGGAGCTG GCCAAGCACG CGGTGTCGGA GGGCACCAAG
6181 GCCGTCACCA AGTACACCAG CTCCAAGTCC GGAGGATCCG GTACCggtgg cggaagtgga
6241 ggtggttcag gttctatgga caaagactgc gaaatgaagc gcaccaccct ggatagccct
6301 ctgggcaagc tggaactgtc tgggtgcgaa cagggcctgc accgtatcat cttcctgggc
6361 aaaggaacat ctgccgccga cgccgtggaa gtgcctgccc cagccgccgt gctgggcgga
6421 ccagagccac tgatgcaggc caccgcctgg ctcaacgcct actttcacca gcctgaggcc
6481 atcgaggagt tccctgtgcc agccctgcac cacccagtgt tccagcagga gagctttacc
6541 cgccaggtgc tgtggaaact gctgaaagtg gtgaagttcg gagaggtcat cagctacagc
6601 cacctggccg ccctggccgg caatcccgcc gccaccgccg ccgtgaaaac cgccctgagc
6661 ggaaatcccg tgcccattct gatcccctgc caccgggtgg tgcagggcga cctggacgtg
6721 gggggctacg agggcgggct cgccgtgaaa gagtggctgc tggcccacga gggccacaga
6781 ctgggcaagc ctgggctggg tGAGATTTCC GGCagcggag atggcgctgg actgatcaac
6841 ggcCGAGCTG ATCCAAAAAA GAAGAGAAAG GTAGATCCAA AAAAGAAGAG AAAGGTAGAT
6901 CCAAAAAAGA AGAGAAAGGT AGGATCCACC GGATCTAGAT AACTGATCAT AATCAGCCAT
6961 ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG
7021 AACGCGGCCG CtggGTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG
7081 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT
7141 ATCATGCGTT AACTAAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
7201 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA
7261 CTCATCAATG TATCTTATCA TGTCTGGAAT TGACTCAAAT GATGTCAATT AGTCTATCAG
7321 AAGCTCATCT GGTCTCCCTT CCGGGGGACA AGACATCCCT GTTTAATATT TAAACAGCAG
7381 TGTTCCCAAA CTGGGTTCTT ATATCCCTTG CTCTGGTCAA CCAGGTTGCA GGGTTTCCTG
7441 TCCTCACAGG AACGAAGTCC CTAAAGAAAC AGTGGCAGCC AGGTTTAGCC CCGGAATTGA
7501 CTGGATTCCT TTTTTAGGGC CCATTGGTAT GGCTTTTTCC CCGTATCCCC CCAGGTGTCT
7561 GCAGGCTCAA AGAGCAGCGA GAAGCGTTCA GAGGAAAGCG ATCCCGTGCC ACCTTCCCCG
7621 TGCCCGGGCT GTCCCCGCAC GCTGCCGGCT CGGGGATGCG GGGGGAGCGC CGGACCGGAG
7681 CGGAGCCCCG GGCGGCTCGC TGCTGCCCCC TAGCGGGGGA GGGACGTAAT TACATCCCTG
7741 GGGGCTTTGG GGGGGGGCTG TCCCTGATAT CTATAACAAG AAATATATAT ATAATAAGTT
7801 ATCACGTAAG TAGAACATGA AATAACAATA TAATTATCGT ATGAGTTAAT CTTAAAGTCA
7861 CGTAAAAGAT AATCATGCGT CATTTTGACT CACGCGGTCG TTATAGTTCA AAATCAGTGA
7921 CACTTACCGC ATTGACAAGC ACGCCTCACG GGAGCTCCAA GCGGCGACTG AGATGTCCTA
7981 AATGCACAGC GACGGATTCG CGCTATTTAG AAAGAGAGAG CAATATTTCA AGAATGCATG
8041 CGTCAATTTT ACGCAGACTA TCTTTCTAGG GTTAATCTAG CTGCATCAGG ATCATATCGT
8101 CGGGTCTTTT TTCCGGCTCA GTCATCGCCC AAGCTGGCGC TATCTGGGCA TCGGGGAGGA
8161 AGAAGCCCGT GCCTTTTCCC GCGAGGTTGA AGCGGCATGG AAAGAGTTTG CCGAGGATGA
8221 CTGCTGCTGC ATTGACGTTG AGCGAAAACG CACGTTTACC ATGATGATTC GGGAAGGTGT
8281 GGCCATGCAC GCCTTTAACG GTGAACTGTT CGTTCAGGCC ACCTGGGATA CCAGTTCGTC
8341 GCGGCTTTTC CGGACACAGT TCCGGATGGT CAGCCCGAAG CGCATCAGCA ACCCGAACAA
8401 TACCGGCGAC AGCCGGAACT GCCGTGCCGG TGTGCAGATT AATGACAGCG GTGCGGCGCT
8461 GGGATATTAC GTCAGCGAGG ACGGGTATCC TGGCTGGATG CCGCAGAAAT GGACATGGAT
8521 ACCCCGTGAG TTACCCGGCG GGCGCGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG
8581 TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA
8641 GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT
8701 TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA
8761 GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
8821 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA
8881 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
8941 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
9001 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
9061 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
9121 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
9181 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
9241 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
9301 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
9361 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC
9421 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC
9481 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA
9541 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA
9601 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT
9661 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA
9721 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA
9781 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC
9841 CCAGTGCTGC AATGATACCG CGAG
//