LOCUS pTG800_EF1alpha-3xFlag- 9864 bp ds-DNA circular 20-APR-2021 DEFINITION . KEYWORDS "creator:Gina Dailey - Darzacq/Tjian Lab" "marker:AmpR, PuroR" FEATURES Location/Qualifiers primer complement(108..126) /label="oTG1552_pBS_diffusion_substrate_300bp" /note="sequence: ctctagcttcccggcaaca" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 CDS complement(650..718) /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 promoter complement(719..823) /label="AmpR promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(802..823) /label="oTG1179_FRT_AmpR_5’" /note="sequence: CTATACTTTCTAGAGAATAGGAACTTCcgcggaacccctatttgtttat" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer complement(821..839) /label="oTG1553_pBS_diffusion_substrate_1kb" /note="sequence: ttttcggggaaatgtgcgc" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 rep_origin complement(849..1303) /label="f1 ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer 1441..1460 /label="oTG1336_M13F_extended" /note="sequence: GTTGTAAAACGACGGCCAGT" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer_bind 1443..1460 /label="M13-fwd" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind 1444..1460 /label="M13 fwd" /ApEinfo_revcolor=#a020f0 /ApEinfo_fwdcolor=#a020f0 misc_feature 1590..1602 /label="PB Terminal repeat" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1637..1652 /label="IR (inverted repeat) truncated?" /ApEinfo_revcolor=#339966 /ApEinfo_fwdcolor=#339966 promoter 2071..2570 /label="PGK promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS 2592..3191 /label="PuroR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 polyA_signal 3224..3448 /label="bGH poly(A) signal" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac promoter 3488..3699 /label="EF-1α core promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 3488..3699 /label="EF-1α core promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 LTR 3712..3980 /label="5' LTR (truncated)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 LTR 3712..3980 /label="5' LTR (truncated)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer 3991..4010 /label="oTG1576_3xFlag-Halo_upstream_F" /note="sequence: CCGGCGCCTACTCTAGCCAC" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS 4012..4014 /label="ATG" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 CDS 4012..6801 /label="Translation 4012-6801" CDS 4015..4080 /label="3xFLAG" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer complement(4057..4081) /label="oTG1613_Flag_RT1_R" /note="sequence: CTAGCttgctattgcttgtcatcgtcatccttgtaatc" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4057..4081) /label="oTG1617_Flag_RT3_R" /note="sequence: CTAGCgccctattgcttgtcatcgtcatccttgtaatc" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4057..4081) /label="oTG1615_Flag_RT2_R" /note="sequence: CTAGCttgctagtgcttgtcatcgtcatccttgtaatc" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 4089..4114 /label="oTG945_60xHalo_F" /note="sequence: CCGGCTCCGGCAGCGGTAGCATGGCAGAAATCGGTACTGGCTTTC" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer 4090..4110 /label="oTG898_H10_Halo_F" /note="sequence: TTCTCACCATCACCATCACCACCACCACCACCATATGGCAGAAATCGGTACTGGC" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 4090..4110 /label="oTG1582_SNAPf_linker_Halo_F" /note="sequence: TGGGCAAGCCTGGGCTGGGTggtagtggtaccggttctATGGCAGAAATCGGTACTGGC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 4090..4110 /label="oTG1573_GSGSKCK_Halo_F" /note="sequence: GGTAGTGGTAGTAAGTGTAAAATGGCAGAAATCGGTACTGGC" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer 4090..4110 /label="oTG778_5'Halo" /note="sequence: ATGGCAGAAATCGGTACTGGC" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e CDS 4090..4980 /label="HaloTag®" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer 4093..4113 /label="oTG1144_inVitro_60xHalo_F" /note="sequence: GGCTCCGGCAGCGGTAGCGCAGAAATCGGTACTGGCTTT" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer 4096..4118 /label="oTG995_Halo_linker_F" /note="sequence: GATCTGGAGGTTCAGGAGGCAGCGGTGGAAGTGAAATCGGTACTGGCTTTCCATT" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer 4096..4118 /label="oTG1280_60mer_linker_Halo_F" /note="sequence: GGAGTGGTAGCGGCAGTATTAAGgaaatcggtactggctttccatt" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer complement(4101..4120) /label="oTG1172_Halo_int_R2" /note="sequence: CGAATGGAAAGCCAGTACCG" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer complement(4106..4125) /label="oTG1214_Halo_int_R_75" /note="sequence: GGGGTCGAATGGAAAGCCAG" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer complement(4129..4148) /label="oTG1096_Halo_int_R" /note="sequence: TCGCCCAGGACTTCCACATA" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer complement(4150..4169) /label="oTG1173_Halo_int_R3" /note="sequence: ACATCGACGTAGTGCATGCG" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer 4311..4332 /label="oTG1513_Halo_qPCR_F1" /note="sequence: ACCAGACCTGGGTTATTTCTTC" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer 4451..4470 /label="oTG1208_Halo_int_F" /note="sequence: AGCGCGTCAAAGGTATTGCA" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer complement(4471..4490) /label="oTG1514_Halo_qPCR_R1" /note="sequence: GGGCGGATGAACTCCATAAA" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer complement(4565..4587) /label="oTG1691_Halo_V167C_R" /note="sequence: GTTCTGATCGATGATCAGCTTGC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4565..4587) /label="oTG1674_Halo_V167C_R" /note="sequence: GTTCTGATCGATGATCAGCTTGC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4574..4599) /label="oTG1676_Halo_G171C_R" /note="sequence: CTCGATAAAAACGTTCTGATCGATGA" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4574..4599) /label="oTG1693_Halo_G171C_R" /note="sequence: CTCGATAAAAACGTTCTGATCGATGA" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 4590..4610 /label="oTG1673_Halo_V167C_F" /note="sequence: tgtTTTATCGAGGGTACGCTGCC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 4590..4610 /label="oTG1690_Halo_V167C_F" /note="sequence: tgtTTTATCGAGGGTACGCTGCC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4592..4611) /label="oTG1678_Halo_M175C_R" /note="sequence: CGGCAGCGTACCCTCGATAA" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4592..4611) /label="oTG1695_Halo_M175C_R" /note="sequence: CGGCAGCGTACCCTCGATAA" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 4601..4620 /label="oTG1692_Halo_G171C_F" /note="sequence: tgtACGCTGCCGATGGGTGTC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 4601..4620 /label="oTG1675_Halo_G171C_F" /note="sequence: tgtACGCTGCCGATGGGTGTC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 4615..4632 /label="oTG1694_Halo_M175C_F" /note="sequence: tgtGGTGTCGTCCGCCCGCTG" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 4615..4632 /label="oTG1677_Halo_M175C_F" /note="sequence: tgtGGTGTCGTCCGCCCGCTG" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(4629..4650) /label="oTG1207_Halo_int_R" /note="sequence: GTCCATCTCGACTTCAGTCAGC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 4636..4657 /label="oTG1515_Halo_qPCR_F2" /note="sequence: GAAGTCGAGATGGACCATTACC" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer complement(4758..4777) /label="oTG1516_Halo_qPCR_R2" /note="sequence: GCAGCCAGTCCATGTATTCT" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer 4871..4890 /label="oTG1095_Halo_int_F" /note="sequence: ACTGCAAGGCTGTGGACATC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 4898..4920 /label="oTG979_3'_Halo_seq" /note="sequence: GTCTGAATCTGCTGCAAGAAGAC" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer complement(4948..4965) /label="oTG1312_Halo_tubUTR_R" /note="sequence: cgcgacgcttagtgtgccgcTTACGTCGACAGCCAGCGCGC" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer complement(4960..4979) /label="oTG1261_ParB2_Halo_stop_R" /note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer complement(4960..4979) /label="oTG1261_ParB2_Halo_stop_R" /note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(4961..4980) /label="oTG779_Halo-stop_pETSUMO" /note="sequence: GCTCCAATTGGGTACCCATGGAGCCGGAAATCTCGAGCGTCG" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer complement(4961..4980) /label="oTG793_3'Halo_R" /note="sequence: GCCGGAAATCTCGAGCGTCG" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer complement(4961..4982) /label="oTG1583_Halo_noNLS_R" /note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCgccggaaatctcgagcgtcg" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 4972..4991 /label="Gibson overhang" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer 4972..4991 /label="oTG1618_SNAP-3xNLS_gBlock_F" /note="sequence: ATTTCCGGCggtagtggtac" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a misc_feature 4999..5544 /label="SNAPf" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 CDS 4999..5544 /label="Translation 4999-5544" primer 5550..5569 /label="oTG1683_3xNLS-P2A-T2A_KpnI_F" /note="sequence: atttccggcggtagtggtaccTCAGGAAGTGGAGATGGCG" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a misc_feature 5602..5670 /label="3xNLS" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 5677..5694 /label="oTG1684_P2A-T2A_KpnI_F" /note="sequence: atttccggcggtagtggtaccGGCAGCGGCGCCACAAAC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a misc_feature 5677..5742 /label="P2A" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 5761..5823 /label="T2A" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer complement(5806..5832) /label="oTG1685_P2A-T2A_KpnI_R" /note="sequence: tttgtccatagaaccggtaccTGGGCCGGGATTTTCCTC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a CDS 5830..6207 /label="H2be" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 CDS 5830..6207 /label="Translation 5830-6207" primer complement(6248..6270) /label="oTG1619_SNAP-3xNLS_gBlock_R" /note="sequence: gcagtctttgtccatagaacctg" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a misc_feature 6250..6270 /label="Gibson overhang" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer 6250..6277 /label="oTG1577_Halo_linker_SNAPf_F" /note="sequence: CGACGCTCGAGATTTCCGGCggtagtggtaccggttctATGGACAAAGACTGCGAAATGA" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer 6256..6278 /label="oTG1562_FKBP-SNAPf_F2" /note="sequence: atggacaaagactgcgaaatgaa" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer 6256..6278 /label="oTG1580_Kozak_SNAPf_F" /note="sequence: CCGGCGCCTACTCTAGCCACCatggacaaagactgcgaaatgaa" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 CDS 6256..6801 /label="SNAPf" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd gene 6256..6801 /label="SNAP26f" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS 6256..6801 /label="Translation 6256-6801" source 6256..6801 /label="source" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(6782..6801) /label="oTG1581_SNAPf_3’_R" /note="sequence: acccagcccaggcttgccca" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(6782..6801) /label="oTG1563_FKBP-SNAPf_R2" /note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer complement(6782..6802) /label="oTG1578_SNAPf_noNLS_R" /note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCacccagcccaggcttgccca" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer complement(6782..6821) /label="oTG1563_FKBP-SNAPf_R2" /note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 CDS 6802..6813 /label="HaloTag®" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd CDS 6802..6942 /label="Translation 6802-6942" primer 6817..6837 /label="oTG1415_7-SspB-p65-Fwd-OptoFly-GA" /note="sequence: ggagatggcgctggactgatc" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 CDS 6817..6840 /label="GDGAGLIN linker" /ApEinfo_revcolor=#cd4489 /ApEinfo_fwdcolor=#cd4489 misc_feature 6843..6843 /label="add_after" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS 6853..6873 /label="SV40 NLS" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 CDS 6877..6897 /label="SV40 NLS" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 CDS 6901..6921 /label="SV40 NLS" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 primer 6920..6944 /label="oTG967_pHalo-Stop_F" /note="sequence: TAGGATCCACCGGATCTAGATAACT" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer complement(6927..6955) /label="oTG1579_3xNLS_downstream_R" /note="sequence: TGATTATGATCAGTTATCTAGATCCGGTG" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 6936..6946 /label="stop codons" /ApEinfo_revcolor=#ff0000 /ApEinfo_fwdcolor=#ff0000 misc_feature 7023..7023 /label="add_before" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS 7023..7040 /label="MCS" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc polyA_signal 7156..7277 /label="SV40 poly(A) signal" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer complement(7249..7277) /label="oTG885_SV40_pA_F" /note="sequence: TAAGATACATTGATGAGTTTGGACAAACC" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature complement(8037..8055) /label="IR (inverted repeat)" /ApEinfo_revcolor=#339966 /ApEinfo_fwdcolor=#339966 misc_feature complement(8059..8071) /label="PB Terminal repeat" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind complement(8562..8578) /label="M13 rev" /ApEinfo_revcolor=#a020f0 /ApEinfo_fwdcolor=#a020f0 protein_bind 8586..8602 /label="lac operator" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b promoter complement(8610..8616) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter complement(8617..8634) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(8628..8649) /label="oTG980_Vector-Fwd-GA" /note="sequence: ccggatgacagtgacgaggagaactgaACCCCAGGCTTTacacTTTATG" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e promoter complement(8635..8640) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 protein_bind 8655..8676 /label="CAP binding site" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b primer 8707..8727 /label="oTG1156_mCherry-LacI-Rev" /note="sequence: AAGCTTCCATTCGGGAAACCTGTCGtgCCAG" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer 8727..8747 /label="oTG1089_Rev-LacI-NLS-NOSTOP-GA" /note="sequence: AACCTTCCTCTTCTTCTTAGGAGGCTGCATTAATGAATCGGCCA" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer 8727..8748 /label="oTG1084_Rev-LacI-GA" /note="sequence: TCAAACCTTCCTCTtcTTcTTaGgagGCTGCATTAATGAATCGGCCAA" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer 8784..8801 /label="oTG775_pET_BbvCI_insert_seq" /note="sequence: TTCCGCTTCCTCGCTCAC" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 rep_origin complement(8964..9551) /label="ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer 9605..9628 /label="oTG1180_AmpR_seq" /note="sequence: ACGTTAAGGGATTTTGGTCATGAG" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(9691..9721) /label="oTG1554_pBS_diffusion_substrate_3kb" /note="sequence: ctgtcagaccaagtttactcatatatacttt" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer complement(9691..9721) /label="oTG1177_FRT_AmpR_3’flanking" /note="sequence: CTATACTTTCTAGAGAATAGGAACTTCctgtcagaccaagtttactcatatatacttt" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer 9692..9721 /label="oTG1182_E75_AmpR_F" /note="sequence: gactaactgccactcgcagcgcgcagatcgAAGTATATATGAGTAAACTTGGTCTGACAG" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer 9721..9745 /label="oTG1549_pBS_849_adapter" /note="sequence: GCTGTGTCTTATTACACACAGTCGttaccaatgcttaatcagtgaggc" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 CDS complement(join(9722..9864,1..649)) /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 9722..9745 /label="oTG1176_FRT_AmpR_3’" /note="sequence: CTATTCTCTAGAAAGTATAGGAACTTCttaccaatgcttaatcagtgaggc" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer complement(9768..9790) /label="oTG1550_pBS_diffusion_substrate_100bp" /note="sequence: caggcaactatggatgaacgaaa" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 ORIGIN 1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT 901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA 961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT 1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA 1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA 1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG 1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA 1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG 1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG 1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC 1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG 1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG 1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG 1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT 1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA 1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT 1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT 1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT 1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA 1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC 2041 TGGGGGGATA CGGGGAAAAG GCGAACGCGT gggtagggga ggcgcttttc ccaaggcagt 2101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc 2161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct 2221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt 2281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca 2341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct 2401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc 2461 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa 2521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc 2581 caAGCTTCAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC 2641 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG 2701 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG 2761 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA 2821 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG 2881 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC 2941 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA 3001 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC 3061 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA 3121 CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC 3181 CCGGTGCCTG AGCGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC 3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC 3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT 3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG 3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AACGCGATCG CTCCGGTGCC 3481 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC 3541 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC 3601 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG 3661 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC 3721 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC 3781 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA 3841 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC 3901 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT 3961 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCCAC CATGGACTAC 4021 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGACAAG 4081 CTTGGCTCCA TGGCAGAAAT CGGTACTGGC TTTCCATTCG ACCCCCATTA TGTGGAAGTC 4141 CTGGGCGAGC GCATGCACTA CGTCGATGTT GGTCCGCGCG ATGGCACCCC TGTGCTGTTC 4201 CTGCACGGTA ACCCGACCTC CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG 4261 ACCCATCGCT GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA ACCAGACCTG 4321 GGTTATTTCT TCGACGACCA CGTCCGCTTC ATGGATGCCT TCATCGAAGC CCTGGGTCTG 4381 GAAGAGGTCG TCCTGGTCAT TCACGACTGG GGCTCCGCTC TGGGTTTCCA CTGGGCCAAG 4441 CGCAATCCAG AGCGCGTCAA AGGTATTGCA TTTATGGAGT TCATCCGCCC TATCCCGACC 4501 TGGGACGAAT GGCCAGAATT TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC 4561 GGCCGCAAGC TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC GATGGGTGTC 4621 GTCCGCCCGC TGACTGAAGT CGAGATGGAC CATTACCGCG AGCCGTTCCT GAATCCTGTT 4681 GACCGCGAGC CACTGTGGCG CTTCCCAAAC GAGCTGCCAA TCGCCGGTGA GCCAGCGAAC 4741 ATCGTCGCGC TGGTCGAAGA ATACATGGAC TGGCTGCACC AGTCCCCTGT CCCGAAGCTG 4801 CTGTTCTGGG GCACCCCAGG CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA 4861 AGCCTGCCTA ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT GCAAGAAGAC 4921 AACCCGGACC TGATCGGCAG CGAGATCGCG CGCTGGCTGT CGACGCTCGA GATTTCCGGC 4981 ggtagtggta caggttctAT GGATAAAGAC TGTGAGATGA AGCGGACAAC CCTTGATTCC 5041 CCTCTGGGGA AACTGGAGCT CAGTGGGTGC GAACAGGGGC TGCACAGAAT TATATTTCTT 5101 GGGAAGGGCA CGTCTGCTGC AGATGCTGTC GAGGTCCCAG CCCCGGCTGC CGTGCTTGGT 5161 GGACCAGAGC CTCTCATGCA AGCTACTGCT TGGCTGAACG CGTATTTCCA CCAACCTGAA 5221 GCGATAGAAG AGTTCCCTGT TCCTGCTCTG CACCACCCGG TTTTCCAACA GGAATCTTTC 5281 ACGAGACAAG TATTGTGGAA ACTTCTCAAA GTGGTCAAAT TCGGTGAAGT AATATCCTAT 5341 TCACACCTGG CAGCTCTCGC CGGGAACCCG GCGGCAACCG CTGCGGTTAA AACTGCACTC 5401 TCTGGTAATC CTGTACCAAT ACTTATACCG TGCCACAGGG TTGTCCAAGG CGACTTGGAC 5461 GTTGGAGGGT ATGAAGGGGG CCTCGCTGTG AAAGAGTGGC TTCTCGCGCA CGAAGGTCAT 5521 CGGCTCGGTA AGCCAGGGCT CGGGGAAATC TCAGGAAGTG GAGATGGCGC CGGATTGATC 5581 AATGGACGGG CAGATgctag cCCGAAGAAA AAGCGGAAGG TCGATCCCAA AAAGAAGAGG 5641 AAAGTGGACC CAAAGAAGAA ACGGAAGGTA catatgggca gcggcgccac aaacttctct 5701 ctgctaaagc aagcaggtga tgttgaagaa aaccccgggc ctACTAGTGC TGGAAAGCTT 5761 ggctccggcg agggcagggg aagtcttcta acatgcgggg acgtggagga aaatcccggc 5821 ccaGgtaccA TGCCTGAGCC TGCGAAGTCC GCTCCCGCCC CGAAGAAGGG CTCCAAGAAG 5881 GCCGTCACCA AGGCCCAGAA GAAGGACGGC AAGAAGCGCA AGCGCAGCCG CAAGGAGAGC 5941 TACTCGGTGT ACGTGTACAA GGTGCTGAAA CAAGTGCACC CCGACACGGG CATCTCCTCC 6001 AAGGCCATGG GCATCATGAA CTCGTTCGTG AACGACATCT TCGAGCGCAT CGCGGGCGAG 6061 GCGTCCCGCC TGGCGCATTA CAACAAGCGC TCGACCATCA CGTCCCGGGA GATCCAGACG 6121 GCCGTGCGCC TGCTGCTGCC CGGGGAGCTG GCCAAGCACG CGGTGTCGGA GGGCACCAAG 6181 GCCGTCACCA AGTACACCAG CTCCAAGTCC GGAGGATCCG GTACCggtgg cggaagtgga 6241 ggtggttcag gttctatgga caaagactgc gaaatgaagc gcaccaccct ggatagccct 6301 ctgggcaagc tggaactgtc tgggtgcgaa cagggcctgc accgtatcat cttcctgggc 6361 aaaggaacat ctgccgccga cgccgtggaa gtgcctgccc cagccgccgt gctgggcgga 6421 ccagagccac tgatgcaggc caccgcctgg ctcaacgcct actttcacca gcctgaggcc 6481 atcgaggagt tccctgtgcc agccctgcac cacccagtgt tccagcagga gagctttacc 6541 cgccaggtgc tgtggaaact gctgaaagtg gtgaagttcg gagaggtcat cagctacagc 6601 cacctggccg ccctggccgg caatcccgcc gccaccgccg ccgtgaaaac cgccctgagc 6661 ggaaatcccg tgcccattct gatcccctgc caccgggtgg tgcagggcga cctggacgtg 6721 gggggctacg agggcgggct cgccgtgaaa gagtggctgc tggcccacga gggccacaga 6781 ctgggcaagc ctgggctggg tGAGATTTCC GGCagcggag atggcgctgg actgatcaac 6841 ggcCGAGCTG ATCCAAAAAA GAAGAGAAAG GTAGATCCAA AAAAGAAGAG AAAGGTAGAT 6901 CCAAAAAAGA AGAGAAAGGT AGGATCCACC GGATCTAGAT AACTGATCAT AATCAGCCAT 6961 ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG 7021 AACGCGGCCG CtggGTCGAC AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG 7081 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT 7141 ATCATGCGTT AACTAAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC 7201 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA 7261 CTCATCAATG TATCTTATCA TGTCTGGAAT TGACTCAAAT GATGTCAATT AGTCTATCAG 7321 AAGCTCATCT GGTCTCCCTT CCGGGGGACA AGACATCCCT GTTTAATATT TAAACAGCAG 7381 TGTTCCCAAA CTGGGTTCTT ATATCCCTTG CTCTGGTCAA CCAGGTTGCA GGGTTTCCTG 7441 TCCTCACAGG AACGAAGTCC CTAAAGAAAC AGTGGCAGCC AGGTTTAGCC CCGGAATTGA 7501 CTGGATTCCT TTTTTAGGGC CCATTGGTAT GGCTTTTTCC CCGTATCCCC CCAGGTGTCT 7561 GCAGGCTCAA AGAGCAGCGA GAAGCGTTCA GAGGAAAGCG ATCCCGTGCC ACCTTCCCCG 7621 TGCCCGGGCT GTCCCCGCAC GCTGCCGGCT CGGGGATGCG GGGGGAGCGC CGGACCGGAG 7681 CGGAGCCCCG GGCGGCTCGC TGCTGCCCCC TAGCGGGGGA GGGACGTAAT TACATCCCTG 7741 GGGGCTTTGG GGGGGGGCTG TCCCTGATAT CTATAACAAG AAATATATAT ATAATAAGTT 7801 ATCACGTAAG TAGAACATGA AATAACAATA TAATTATCGT ATGAGTTAAT CTTAAAGTCA 7861 CGTAAAAGAT AATCATGCGT CATTTTGACT CACGCGGTCG TTATAGTTCA AAATCAGTGA 7921 CACTTACCGC ATTGACAAGC ACGCCTCACG GGAGCTCCAA GCGGCGACTG AGATGTCCTA 7981 AATGCACAGC GACGGATTCG CGCTATTTAG AAAGAGAGAG CAATATTTCA AGAATGCATG 8041 CGTCAATTTT ACGCAGACTA TCTTTCTAGG GTTAATCTAG CTGCATCAGG ATCATATCGT 8101 CGGGTCTTTT TTCCGGCTCA GTCATCGCCC AAGCTGGCGC TATCTGGGCA TCGGGGAGGA 8161 AGAAGCCCGT GCCTTTTCCC GCGAGGTTGA AGCGGCATGG AAAGAGTTTG CCGAGGATGA 8221 CTGCTGCTGC ATTGACGTTG AGCGAAAACG CACGTTTACC ATGATGATTC GGGAAGGTGT 8281 GGCCATGCAC GCCTTTAACG GTGAACTGTT CGTTCAGGCC ACCTGGGATA CCAGTTCGTC 8341 GCGGCTTTTC CGGACACAGT TCCGGATGGT CAGCCCGAAG CGCATCAGCA ACCCGAACAA 8401 TACCGGCGAC AGCCGGAACT GCCGTGCCGG TGTGCAGATT AATGACAGCG GTGCGGCGCT 8461 GGGATATTAC GTCAGCGAGG ACGGGTATCC TGGCTGGATG CCGCAGAAAT GGACATGGAT 8521 ACCCCGTGAG TTACCCGGCG GGCGCGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG 8581 TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA 8641 GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT 8701 TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA 8761 GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC 8821 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA 8881 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT 8941 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA 9001 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT 9061 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 9121 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC 9181 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC 9241 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA 9301 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT 9361 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC 9421 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 9481 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 9541 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 9601 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 9661 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 9721 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 9781 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 9841 CCAGTGCTGC AATGATACCG CGAG //