# Nova Proteins Direct Synaptic Integration of Somatostatin Interneurons Through Activity-Dependent Alternative Splicing

## Abstract

Somatostatin interneurons are the earliest born population of cortical inhibitory cells. They are crucial to support normal brain development and function; however, the mechanisms underlying their integration into nascent cortical circuitry are not well understood. In this study, we begin by demonstrating that the maturation of somatostatin interneurons is activity dependent. We then investigated the relationship between activity, alternative splicing and synapse formation within this population. Specifically, we discovered that the Nova family of RNA-binding proteins are activity-dependent and are essential for the maturation of somatostatin interneurons, as well as their afferent and efferent connectivity. Within this population, Nova2 preferentially mediates the alternative splicing of genes required for axonal formation and synaptic function independently from its effect on gene expression. Hence, our work demonstrates that the Nova family of proteins through alternative splicing are centrally involved in coupling developmental neuronal activity to cortical circuit formation.


## Citation

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Leena Ali Ibrahim, Brie Wamsley, Norah Alghamdi, Nusrath Yusuf, Elaine Sevier, Ariel Hairston, Mia Sherer, Xavier Hubert Jaglin, Qing Xu, Lihua Guo, Alireza Khodadadi-Jamayran, Emilia Favuzzi, Yuan Yuan, Jordane Dimidschstein, Robert B Darnell, Gordon Fishell (2023) Nova proteins direct synaptic integration of somatostatin interneurons through activity-dependent alternative splicing eLife 12:e86842
https://doi.org/10.7554/eLife.86842

## Questions

If you have any questions, please feel free to contact the authors:
leena.ibrahim@kaust.edu.sa and gordon_fishell@hms.harvard.edu