ptg825-ef1alpha-snapf-60mer-t2a-p2a-halo-snapf.gb
LOCUS pTG825_EF1alpha-SNAPf-6 9789 bp ds-DNA circular 29-JUL-2021
DEFINITION .
KEYWORDS "creator:Gina Dailey - Darzacq/Tjian Lab" "marker:AmpR, PuroR"
FEATURES Location/Qualifiers
primer complement(108..126)
/label="oTG1552_pBS_diffusion_substrate_300bp"
/note="sequence: ctctagcttcccggcaaca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS complement(650..718)
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter complement(719..823)
/label="AmpR promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(802..823)
/label="oTG1179_FRT_AmpR_5’"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCcgcggaacccctatttgtttat"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(821..839)
/label="oTG1553_pBS_diffusion_substrate_1kb"
/note="sequence: ttttcggggaaatgtgcgc"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
rep_origin complement(849..1303)
/label="f1 ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 1441..1460
/label="oTG1336_M13F_extended"
/note="sequence: GTTGTAAAACGACGGCCAGT"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind 1443..1460
/label="M13-fwd"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 1444..1460
/label="M13 fwd"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
misc_feature 1590..1602
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 1637..1652
/label="IR (inverted repeat) truncated?"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
promoter 2071..2570
/label="PGK promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS 2592..3191
/label="PuroR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
polyA_signal 3224..3448
/label="bGH poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 3991..4010
/label="oTG1576_3xFlag-Halo_upstream_F"
/note="sequence: CCGGCGCCTACTCTAGCCAC"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 3991..4036
/label="oTG1729_Kozak_SNAPfv2_F"
/note="sequence: CCGGCGCCTACTCTAGCCACCatggataaagactgtgagatgaagc"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 4012..4557
/label="SNAPf"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
CDS 4012..6846
/label="Translation 4012-6846"
primer 4563..4582
/label="oTG1683_3xNLS-P2A-T2A_KpnI_F"
/note="sequence: atttccggcggtagtggtaccTCAGGAAGTGGAGATGGCG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4590..4639
/label="oTG1620_HaloSNAP60mer-T2AP2A-SNAP_F"
/note="sequence: GATCAATGGACGGGCAGATgctagcATGAAAATGGAAGAGCTGTTCAAGA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4614..4639
/label="oTG909_60mer_pcDNA_F"
/note="sequence: CCACTAGTCCAGTGTGGTGGCCACCATGAAAATGGAAGAGCTGTTCAAGA"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer 4614..4639
/label="oTG1622_HaloSNAP3xN-TP2A-60merSNAP_F"
/note="sequence: gaaaatcccggcccaGgtaccATGAAAATGGAAGAGCTGTTCAAGA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 4615..5229
/label="60mer"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer 4618..4639
/label="oTG936_60xGFP_F"
/note="sequence: AAAATGGAAGAGCTGTTCAAGA"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 4618..4643
/label="oTG977_Linker_ctGFP_F"
/note="sequence: GTTCAGGAGGCAGCGGTGGAAGTAAAATGGAAGAGCTGTTCAAGAAACA"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer complement(5211..5229)
/label="oTG1621_HaloSNAP60mer-T2AP2A-SNAP_R"
/note="sequence: ttgtggcgccgctgcccatatgTTCGGTGCAGCCACGAATC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5211..5229)
/label="oTG1623_HaloSNAP60mer-TP2A-SNAP_R"
/note="sequence: tccacttccgccaccggtaccTTCGGTGCAGCCACGAATC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5211..5231)
/label="oTG1623_HaloSNAP60mer-TP2A-SNAP_R"
/note="sequence: tccacttccgccaccggtaccTTCGGTGCAGCCACGAATC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5211..5251)
/label="oTG1621_HaloSNAP60mer-T2AP2A-SNAP_R"
/note="sequence: ttgtggcgccgctgcccatatgTTCGGTGCAGCCACGAATC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5229..5247
/label="oTG1725_SNAPf-3xNLS_P2A_F"
/note="sequence: AGAAAGGTAGGATCCACCGGAcatatgggcagcggcgcc"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5236..5253
/label="oTG1684_P2A-T2A_KpnI_F"
/note="sequence: atttccggcggtagtggtaccGGCAGCGGCGCCACAAAC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 5236..5301
/label="P2A"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 5320..5382
/label="T2A"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
primer complement(5365..5388)
/label="oTG1685_P2A-T2A_KpnI_R"
/note="sequence: tttgtccatagaaccggtaccTGGGCCGGGATTTTCCTC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5369..5409
/label="oTG1730_T2A_Halo_F"
/note="sequence: AAAATCCCGGCCCAGGTACCatggcagaaatcggtactggc"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5371..5388)
/label="oTG1726_T2A-KpnI_R"
/note="sequence: ggtacctgggccgggatt"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5388..5413
/label="oTG945_60xHalo_F"
/note="sequence: CCGGCTCCGGCAGCGGTAGCATGGCAGAAATCGGTACTGGCTTTC"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 5389..5409
/label="oTG1582_SNAPf_linker_Halo_F"
/note="sequence: TGGGCAAGCCTGGGCTGGGTggtagtggtaccggttctATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 5389..5409
/label="oTG778_5'Halo"
/note="sequence: ATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 5389..5409
/label="oTG1573_GSGSKCK_Halo_F"
/note="sequence: GGTAGTGGTAGTAAGTGTAAAATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer 5389..5409
/label="oTG898_H10_Halo_F"
/note="sequence: TTCTCACCATCACCATCACCACCACCACCACCATATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
CDS 5389..6279
/label="HaloTag®"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 5392..5412
/label="oTG1144_inVitro_60xHalo_F"
/note="sequence: GGCTCCGGCAGCGGTAGCGCAGAAATCGGTACTGGCTTT"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer 5395..5417
/label="oTG1280_60mer_linker_Halo_F"
/note="sequence: GGAGTGGTAGCGGCAGTATTAAGgaaatcggtactggctttccatt"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 5395..5417
/label="oTG995_Halo_linker_F"
/note="sequence: GATCTGGAGGTTCAGGAGGCAGCGGTGGAAGTGAAATCGGTACTGGCTTTCCATT"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer complement(5400..5419)
/label="oTG1172_Halo_int_R2"
/note="sequence: CGAATGGAAAGCCAGTACCG"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(5405..5424)
/label="oTG1214_Halo_int_R_75"
/note="sequence: GGGGTCGAATGGAAAGCCAG"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(5428..5447)
/label="oTG1096_Halo_int_R"
/note="sequence: TCGCCCAGGACTTCCACATA"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(5449..5468)
/label="oTG1173_Halo_int_R3"
/note="sequence: ACATCGACGTAGTGCATGCG"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 5610..5631
/label="oTG1513_Halo_qPCR_F1"
/note="sequence: ACCAGACCTGGGTTATTTCTTC"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer 5750..5769
/label="oTG1208_Halo_int_F"
/note="sequence: AGCGCGTCAAAGGTATTGCA"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(5770..5789)
/label="oTG1514_Halo_qPCR_R1"
/note="sequence: GGGCGGATGAACTCCATAAA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(5864..5886)
/label="oTG1674_Halo_V167C_R"
/note="sequence: GTTCTGATCGATGATCAGCTTGC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5864..5886)
/label="oTG1691_Halo_V167C_R"
/note="sequence: GTTCTGATCGATGATCAGCTTGC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5873..5898)
/label="oTG1693_Halo_G171C_R"
/note="sequence: CTCGATAAAAACGTTCTGATCGATGA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5873..5898)
/label="oTG1676_Halo_G171C_R"
/note="sequence: CTCGATAAAAACGTTCTGATCGATGA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5889..5909
/label="oTG1673_Halo_V167C_F"
/note="sequence: tgtTTTATCGAGGGTACGCTGCC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5889..5909
/label="oTG1690_Halo_V167C_F"
/note="sequence: tgtTTTATCGAGGGTACGCTGCC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5891..5910)
/label="oTG1678_Halo_M175C_R"
/note="sequence: CGGCAGCGTACCCTCGATAA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5891..5910)
/label="oTG1695_Halo_M175C_R"
/note="sequence: CGGCAGCGTACCCTCGATAA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5900..5919
/label="oTG1692_Halo_G171C_F"
/note="sequence: tgtACGCTGCCGATGGGTGTC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5900..5919
/label="oTG1675_Halo_G171C_F"
/note="sequence: tgtACGCTGCCGATGGGTGTC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5914..5931
/label="oTG1694_Halo_M175C_F"
/note="sequence: tgtGGTGTCGTCCGCCCGCTG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5914..5931
/label="oTG1677_Halo_M175C_F"
/note="sequence: tgtGGTGTCGTCCGCCCGCTG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(5928..5949)
/label="oTG1207_Halo_int_R"
/note="sequence: GTCCATCTCGACTTCAGTCAGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 5935..5956
/label="oTG1515_Halo_qPCR_F2"
/note="sequence: GAAGTCGAGATGGACCATTACC"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer complement(6057..6076)
/label="oTG1516_Halo_qPCR_R2"
/note="sequence: GCAGCCAGTCCATGTATTCT"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 6170..6189
/label="oTG1095_Halo_int_F"
/note="sequence: ACTGCAAGGCTGTGGACATC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 6197..6219
/label="oTG979_3'_Halo_seq"
/note="sequence: GTCTGAATCTGCTGCAAGAAGAC"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer complement(6247..6264)
/label="oTG1312_Halo_tubUTR_R"
/note="sequence: cgcgacgcttagtgtgccgcTTACGTCGACAGCCAGCGCGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(6259..6278)
/label="oTG1261_ParB2_Halo_stop_R"
/note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(6259..6278)
/label="oTG1261_ParB2_Halo_stop_R"
/note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(6260..6279)
/label="oTG779_Halo-stop_pETSUMO"
/note="sequence: GCTCCAATTGGGTACCCATGGAGCCGGAAATCTCGAGCGTCG"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer complement(6260..6279)
/label="oTG793_3'Halo_R"
/note="sequence: GCCGGAAATCTCGAGCGTCG"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer complement(6260..6281)
/label="oTG1583_Halo_noNLS_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCgccggaaatctcgagcgtcg"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 6260..6319
/label="oTG1577_Halo_linker_SNAPf_F"
/note="sequence: CGACGCTCGAGATTTCCGGCggtagtggtaccggttctATGGACAAAGACTGCGAAATGA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 6271..6290
/label="oTG1618_SNAP-3xNLS_gBlock_F"
/note="sequence: ATTTCCGGCggtagtggtac"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 6298..6320
/label="oTG1562_FKBP-SNAPf_F2"
/note="sequence: atggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 6298..6320
/label="oTG1580_Kozak_SNAPf_F"
/note="sequence: CCGGCGCCTACTCTAGCCACCatggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
CDS 6298..6843
/label="SNAPf"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
gene 6298..6843
/label="SNAP26f"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
source 6298..6843
/label="source"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(6385..6405)
/label="oTG1722_SNAPf_v2_int_R"
/note="sequence: tttgcccaggaagatgatacg"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(6824..6843)
/label="oTG1581_SNAPf_3’_R"
/note="sequence: acccagcccaggcttgccca"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(6824..6843)
/label="oTG1563_FKBP-SNAPf_R2"
/note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(6824..6843)
/label="oTG1578_SNAPf_noNLS_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCacccagcccaggcttgccca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(6824..6871)
/label="oTG1731_SNAPf_stop_BamHI_R"
/note="sequence: TCAGTTATCTAGATCCGGTGGATCCTTAacccagcccaggcttgccca"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 6845..6869
/label="oTG967_pHalo-Stop_F"
/note="sequence: TAGGATCCACCGGATCTAGATAACT"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 6846..6869
/label="oTG967_pHalo-Stop_F"
/note="sequence: TAGGATCCACCGGATCTAGATAACT"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(6852..6880)
/label="oTG1579_3xNLS_downstream_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTG"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 6861..6871
/label="stop codons"
/ApEinfo_revcolor=#ff0000
/ApEinfo_fwdcolor=#ff0000
misc_feature 6948..6948
/label="add_before"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 6948..6965
/label="MCS"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
polyA_signal 7081..7202
/label="SV40 poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(7174..7202)
/label="oTG885_SV40_pA_F"
/note="sequence: TAAGATACATTGATGAGTTTGGACAAACC"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature complement(7962..7980)
/label="IR (inverted repeat)"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
misc_feature complement(7984..7996)
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind complement(8487..8503)
/label="M13 rev"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
protein_bind 8511..8527
/label="lac operator"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
promoter complement(8535..8541)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(8542..8559)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(8553..8574)
/label="oTG980_Vector-Fwd-GA"
/note="sequence: ccggatgacagtgacgaggagaactgaACCCCAGGCTTTacacTTTATG"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
promoter complement(8560..8565)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
protein_bind 8580..8601
/label="CAP binding site"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
primer 8632..8652
/label="oTG1156_mCherry-LacI-Rev"
/note="sequence: AAGCTTCCATTCGGGAAACCTGTCGtgCCAG"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 8652..8672
/label="oTG1089_Rev-LacI-NLS-NOSTOP-GA"
/note="sequence: AACCTTCCTCTTCTTCTTAGGAGGCTGCATTAATGAATCGGCCA"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 8652..8673
/label="oTG1084_Rev-LacI-GA"
/note="sequence: TCAAACCTTCCTCTtcTTcTTaGgagGCTGCATTAATGAATCGGCCAA"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 8709..8726
/label="oTG775_pET_BbvCI_insert_seq"
/note="sequence: TTCCGCTTCCTCGCTCAC"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
rep_origin complement(8889..9476)
/label="ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 9530..9553
/label="oTG1180_AmpR_seq"
/note="sequence: ACGTTAAGGGATTTTGGTCATGAG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(9616..9646)
/label="oTG1554_pBS_diffusion_substrate_3kb"
/note="sequence: ctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer complement(9616..9646)
/label="oTG1177_FRT_AmpR_3’flanking"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer 9617..9646
/label="oTG1182_E75_AmpR_F"
/note="sequence: gactaactgccactcgcagcgcgcagatcgAAGTATATATGAGTAAACTTGGTCTGACAG"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer 9646..9670
/label="oTG1549_pBS_849_adapter"
/note="sequence: GCTGTGTCTTATTACACACAGTCGttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
CDS complement(join(9647..9789,1..649))
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 9647..9670
/label="oTG1176_FRT_AmpR_3’"
/note="sequence: CTATTCTCTAGAAAGTATAGGAACTTCttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(9693..9715)
/label="oTG1550_pBS_diffusion_substrate_100bp"
/note="sequence: caggcaactatggatgaacgaaa"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC
61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG
121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA
181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA
241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA
301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA
361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA
421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG
481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG
541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG
601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG
661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC
721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT
901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA
961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA
1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA
1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG
1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA
1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG
1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG
1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC
1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG
1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG
1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG
1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT
1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA
1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT
1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT
1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT
1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA
1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC
2041 TGGGGGGATA CGGGGAAAAG GCGAACGCGT gggtagggga ggcgcttttc ccaaggcagt
2101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
2161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
2221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
2281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
2341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
2401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
2461 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
2521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
2581 caAGCTTCAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC
2641 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG
2701 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG
2761 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA
2821 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG
2881 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC
2941 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA
3001 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC
3061 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA
3121 CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC
3181 CCGGTGCCTG AGCGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AACGCGATCG CTCCGGTGCC
3481 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC
3541 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC
3601 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG
3661 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC
3721 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC
3781 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA
3841 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC
3901 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT
3961 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCCAC CATGGATAAA
4021 GACTGTGAGA TGAAGCGGAC AACCCTTGAT TCCCCTCTGG GGAAACTGGA GCTCAGTGGG
4081 TGCGAACAGG GGCTGCACAG AATTATATTT CTTGGGAAGG GCACGTCTGC TGCAGATGCT
4141 GTCGAGGTCC CAGCCCCGGC TGCCGTGCTT GGTGGACCAG AGCCTCTCAT GCAAGCTACT
4201 GCTTGGCTGA ACGCGTATTT CCACCAACCT GAAGCGATAG AAGAGTTCCC TGTTCCTGCT
4261 CTGCACCACC CGGTTTTCCA ACAGGAATCT TTCACGAGAC AAGTATTGTG GAAACTTCTC
4321 AAAGTGGTCA AATTCGGTGA AGTAATATCC TATTCACACC TGGCAGCTCT CGCCGGGAAC
4381 CCGGCGGCAA CCGCTGCGGT TAAAACTGCA CTCTCTGGTA ATCCTGTACC AATACTTATA
4441 CCGTGCCACA GGGTTGTCCA AGGCGACTTG GACGTTGGAG GGTATGAAGG GGGCCTCGCT
4501 GTGAAAGAGT GGCTTCTCGC GCACGAAGGT CATCGGCTCG GTAAGCCAGG GCTCGGGGAA
4561 ATCTCAGGAA GTGGAGATGG CGCCGGATTG ATCAATGGAC GGGCAGATgc tagcATGAAA
4621 ATGGAAGAGC TGTTCAAGAA ACACAAGATC GTTGCCGTGC TGCGTGCCAA TAGTGTGGAA
4681 GAAGCGAAAA AGAAAGCGCT GGCGGTTTTC CTGGGCGGCG TTCATCTGAT TGAAATTACC
4741 TTTACCGTGC CGGATGCGGA TACCGTGATT AAGGAACTGA GCTTTCTGAA GGAAATGGGC
4801 GCGATTATTG GTGCGGGCAC CGTGACCAGC GTGGAGCAGT GCCGTAAAGC GGTGGAAAGT
4861 GGCGCCGAAT TCATTGTGAG TCCGCACCTG GACGAGGAAA TTAGCCAATT TTGCAAGGAG
4921 AAGGGTGTGT TCTATATGCC AGGCGTTATG ACCCCGACCG AACTGGTGAA AGCCATGAAA
4981 CTGGGCCATA CCATCTTAAA ACTGTTTCCG GGTGAGGTGG TGGGTCCGCA GTTTGTTAAA
5041 GCGATGAAAG GTCCGTTTCC GAATGTGAAA TTTGTGCCAA CCGGCGGTGT TAATCTGGAC
5101 AATGTGTGCG AATGGTTCAA AGCGGGCGTG CTGGCCGTGG GCGTGGGCAG CGCGTTAGTG
5161 AAAGGCACCC CGGTGGAAGT GGCGGAAAAG GCCAAGGCGT TCGTTGAGAA GATTCGTGGC
5221 TGCACCGAAc atatgggcag cggcgccaca aacttctctc tgctaaagca agcaggtgat
5281 gttgaagaaa accccgggcc tACTAGTGCT GGAAAGCTTg gctccggcga gggcagggga
5341 agtcttctaa catgcgggga cgtggaggaa aatcccggcc caGgtaccAT GGCAGAAATC
5401 GGTACTGGCT TTCCATTCGA CCCCCATTAT GTGGAAGTCC TGGGCGAGCG CATGCACTAC
5461 GTCGATGTTG GTCCGCGCGA TGGCACCCCT GTGCTGTTCC TGCACGGTAA CCCGACCTCC
5521 TCCTACGTGT GGCGCAACAT CATCCCGCAT GTTGCACCGA CCCATCGCTG CATTGCTCCA
5581 GACCTGATCG GTATGGGCAA ATCCGACAAA CCAGACCTGG GTTATTTCTT CGACGACCAC
5641 GTCCGCTTCA TGGATGCCTT CATCGAAGCC CTGGGTCTGG AAGAGGTCGT CCTGGTCATT
5701 CACGACTGGG GCTCCGCTCT GGGTTTCCAC TGGGCCAAGC GCAATCCAGA GCGCGTCAAA
5761 GGTATTGCAT TTATGGAGTT CATCCGCCCT ATCCCGACCT GGGACGAATG GCCAGAATTT
5821 GCCCGCGAGA CCTTCCAGGC CTTCCGCACC ACCGACGTCG GCCGCAAGCT GATCATCGAT
5881 CAGAACGTTT TTATCGAGGG TACGCTGCCG ATGGGTGTCG TCCGCCCGCT GACTGAAGTC
5941 GAGATGGACC ATTACCGCGA GCCGTTCCTG AATCCTGTTG ACCGCGAGCC ACTGTGGCGC
6001 TTCCCAAACG AGCTGCCAAT CGCCGGTGAG CCAGCGAACA TCGTCGCGCT GGTCGAAGAA
6061 TACATGGACT GGCTGCACCA GTCCCCTGTC CCGAAGCTGC TGTTCTGGGG CACCCCAGGC
6121 GTTCTGATCC CACCGGCCGA AGCCGCTCGC CTGGCCAAAA GCCTGCCTAA CTGCAAGGCT
6181 GTGGACATCG GCCCGGGTCT GAATCTGCTG CAAGAAGACA ACCCGGACCT GATCGGCAGC
6241 GAGATCGCGC GCTGGCTGTC GACGCTCGAG ATTTCCGGCg gtagtggtac cggttctatg
6301 gacaaagact gcgaaatgaa gcgcaccacc ctggatagcc ctctgggcaa gctggaactg
6361 tctgggtgcg aacagggcct gcaccgtatc atcttcctgg gcaaaggaac atctgccgcc
6421 gacgccgtgg aagtgcctgc cccagccgcc gtgctgggcg gaccagagcc actgatgcag
6481 gccaccgcct ggctcaacgc ctactttcac cagcctgagg ccatcgagga gttccctgtg
6541 ccagccctgc accacccagt gttccagcag gagagcttta cccgccaggt gctgtggaaa
6601 ctgctgaaag tggtgaagtt cggagaggtc atcagctaca gccacctggc cgccctggcc
6661 ggcaatcccg ccgccaccgc cgccgtgaaa accgccctga gcggaaatcc cgtgcccatt
6721 ctgatcccct gccaccgggt ggtgcagggc gacctggacg tggggggcta cgagggcggg
6781 ctcgccgtga aagagtggct gctggcccac gagggccaca gactgggcaa gcctgggctg
6841 ggttaaGGAT CCACCGGATC TAGATAACTG ATCATAATCA GCCATACCAC ATTTGTAGAG
6901 GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAACGC GGCCGCtggG
6961 TCGACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG
7021 TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCGTTAACTA
7081 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA
7141 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT
7201 TATCATGTCT GGAATTGACT CAAATGATGT CAATTAGTCT ATCAGAAGCT CATCTGGTCT
7261 CCCTTCCGGG GGACAAGACA TCCCTGTTTA ATATTTAAAC AGCAGTGTTC CCAAACTGGG
7321 TTCTTATATC CCTTGCTCTG GTCAACCAGG TTGCAGGGTT TCCTGTCCTC ACAGGAACGA
7381 AGTCCCTAAA GAAACAGTGG CAGCCAGGTT TAGCCCCGGA ATTGACTGGA TTCCTTTTTT
7441 AGGGCCCATT GGTATGGCTT TTTCCCCGTA TCCCCCCAGG TGTCTGCAGG CTCAAAGAGC
7501 AGCGAGAAGC GTTCAGAGGA AAGCGATCCC GTGCCACCTT CCCCGTGCCC GGGCTGTCCC
7561 CGCACGCTGC CGGCTCGGGG ATGCGGGGGG AGCGCCGGAC CGGAGCGGAG CCCCGGGCGG
7621 CTCGCTGCTG CCCCCTAGCG GGGGAGGGAC GTAATTACAT CCCTGGGGGC TTTGGGGGGG
7681 GGCTGTCCCT GATATCTATA ACAAGAAATA TATATATAAT AAGTTATCAC GTAAGTAGAA
7741 CATGAAATAA CAATATAATT ATCGTATGAG TTAATCTTAA AGTCACGTAA AAGATAATCA
7801 TGCGTCATTT TGACTCACGC GGTCGTTATA GTTCAAAATC AGTGACACTT ACCGCATTGA
7861 CAAGCACGCC TCACGGGAGC TCCAAGCGGC GACTGAGATG TCCTAAATGC ACAGCGACGG
7921 ATTCGCGCTA TTTAGAAAGA GAGAGCAATA TTTCAAGAAT GCATGCGTCA ATTTTACGCA
7981 GACTATCTTT CTAGGGTTAA TCTAGCTGCA TCAGGATCAT ATCGTCGGGT CTTTTTTCCG
8041 GCTCAGTCAT CGCCCAAGCT GGCGCTATCT GGGCATCGGG GAGGAAGAAG CCCGTGCCTT
8101 TTCCCGCGAG GTTGAAGCGG CATGGAAAGA GTTTGCCGAG GATGACTGCT GCTGCATTGA
8161 CGTTGAGCGA AAACGCACGT TTACCATGAT GATTCGGGAA GGTGTGGCCA TGCACGCCTT
8221 TAACGGTGAA CTGTTCGTTC AGGCCACCTG GGATACCAGT TCGTCGCGGC TTTTCCGGAC
8281 ACAGTTCCGG ATGGTCAGCC CGAAGCGCAT CAGCAACCCG AACAATACCG GCGACAGCCG
8341 GAACTGCCGT GCCGGTGTGC AGATTAATGA CAGCGGTGCG GCGCTGGGAT ATTACGTCAG
8401 CGAGGACGGG TATCCTGGCT GGATGCCGCA GAAATGGACA TGGATACCCC GTGAGTTACC
8461 CGGCGGGCGC GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG
8521 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA
8581 TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC
8641 CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT
8701 GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA
8761 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA
8821 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
8881 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT
8941 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC
9001 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT
9061 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC
9121 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
9181 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA
9241 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG
9301 TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG
9361 CCAGTTACCT TCGGAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
9421 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
9481 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
9541 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA
9601 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
9661 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC
9721 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA
9781 TACCGCGAG
//