LOCUS pTG742_EF1alpha-FKBP-SN 7956 bp ds-DNA circular 17-NOV-2020 DEFINITION . KEYWORDS "creator:Gina Dailey - Darzacq/Tjian Lab" "marker:AmpR, PuroR" FEATURES Location/Qualifiers primer complement(108..126) /label="oTG1552_pBS_diffusion_substrate_300bp" /note="sequence: ctctagcttcccggcaaca" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 CDS complement(650..718) /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 promoter complement(719..823) /label="AmpR promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(802..823) /label="oTG1179_FRT_AmpR_5’" /note="sequence: CTATACTTTCTAGAGAATAGGAACTTCcgcggaacccctatttgtttat" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer complement(821..839) /label="oTG1553_pBS_diffusion_substrate_1kb" /note="sequence: ttttcggggaaatgtgcgc" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 rep_origin complement(849..1303) /label="f1 ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer 1441..1460 /label="oTG1336_M13F_extended" /note="sequence: GTTGTAAAACGACGGCCAGT" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer_bind 1443..1460 /label="M13-fwd" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind 1444..1460 /label="M13 fwd" /ApEinfo_revcolor=#a020f0 /ApEinfo_fwdcolor=#a020f0 misc_feature 1590..1602 /label="PB Terminal repeat" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1637..1652 /label="IR (inverted repeat) truncated?" /ApEinfo_revcolor=#339966 /ApEinfo_fwdcolor=#339966 promoter 2071..2570 /label="PGK promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS 2592..3191 /label="PuroR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 polyA_signal 3224..3448 /label="bGH poly(A) signal" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac promoter 3488..3699 /label="EF-1α core promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 3488..3699 /label="EF-1α core promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 LTR 3712..3980 /label="5' LTR (truncated)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 LTR 3712..3980 /label="5' LTR (truncated)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer 3991..4010 /label="oTG1576_3xFlag-Halo_upstream_F" /note="sequence: CCGGCGCCTACTCTAGCCAC" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer 3991..4034 /label="oTG1560_FKBP-SNAPf_F1" /note="sequence: CCGGCGCCTACTCTAGCCACCATGggagtgcaggtggaaaccat" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 4006..4030 /label="oTG939_pcDNA_FKBP_F" /note="sequence: CCACTAGTCCAGTGTGGTGGCCACCatgggagtgcaggtggaaa" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 CDS 4012..4014 /label="ATG" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 misc_feature 4015..4034 /label="F primer" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd CDS 4015..4335 /label="FKBP" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 CDS 4015..4347 /label="Translation 4015-4347" CDS 4015..5034 /label="Translation 4015-5034" primer complement(4312..4336) /label="oTG940_FKBP_60mer_R" /note="sequence: TCTTGAACAGCTCTTCCATTTTttccagttttagaagctccacatc" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(4328..4347) /label="R primer" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer complement(4328..4370) /label="oTG1561_FKBP-SNAPf_R1" /note="sequence: TTCATTTCGCAGTCTTTGTCCATaccaccagcactttccagtt" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer 4347..4369 /label="oTG1577_Halo_linker_SNAPf_F" /note="sequence: CGACGCTCGAGATTTCCGGCggtagtggtaccggttctATGGACAAAGACTGCGAAATGA" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer 4348..4370 /label="oTG1580_Kozak_SNAPf_F" /note="sequence: CCGGCGCCTACTCTAGCCACCatggacaaagactgcgaaatgaa" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer 4348..4370 /label="oTG1562_FKBP-SNAPf_F2" /note="sequence: atggacaaagactgcgaaatgaa" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 gene 4348..4893 /label="SNAP26f" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS 4348..4893 /label="SNAPf" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd CDS 4348..4893 /label="Translation 4348-4893" source 4348..4893 /label="source" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(4874..4893) /label="oTG1581_SNAPf_3’_R" /note="sequence: acccagcccaggcttgccca" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(4874..4894) /label="oTG1578_SNAPf_noNLS_R" /note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCacccagcccaggcttgccca" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer complement(4874..4913) /label="oTG1563_FKBP-SNAPf_R2" /note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 CDS 4894..4905 /label="HaloTag®" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer 4909..4929 /label="oTG1415_7-SspB-p65-Fwd-OptoFly-GA" /note="sequence: ggagatggcgctggactgatc" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 CDS 4909..4932 /label="GDGAGLIN linker" /ApEinfo_revcolor=#cd4489 /ApEinfo_fwdcolor=#cd4489 misc_feature 4935..4935 /label="add_after" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS 4945..4965 /label="SV40 NLS" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 CDS 4969..4989 /label="SV40 NLS" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 CDS 4993..5013 /label="SV40 NLS" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 primer 5012..5036 /label="oTG967_pHalo-Stop_F" /note="sequence: TAGGATCCACCGGATCTAGATAACT" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer complement(5019..5047) /label="oTG1579_3xNLS_downstream_R" /note="sequence: TGATTATGATCAGTTATCTAGATCCGGTG" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 5028..5038 /label="stop codons" /ApEinfo_revcolor=#ff0000 /ApEinfo_fwdcolor=#ff0000 misc_feature 5115..5115 /label="add_before" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS 5115..5132 /label="MCS" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc polyA_signal 5248..5369 /label="SV40 poly(A) signal" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer complement(5341..5369) /label="oTG885_SV40_pA_F" /note="sequence: TAAGATACATTGATGAGTTTGGACAAACC" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature complement(6129..6147) /label="IR (inverted repeat)" /ApEinfo_revcolor=#339966 /ApEinfo_fwdcolor=#339966 misc_feature complement(6151..6163) /label="PB Terminal repeat" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind complement(6654..6670) /label="M13 rev" /ApEinfo_revcolor=#a020f0 /ApEinfo_fwdcolor=#a020f0 protein_bind 6678..6694 /label="lac operator" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b promoter complement(6702..6708) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter complement(6709..6726) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(6720..6741) /label="oTG980_Vector-Fwd-GA" /note="sequence: ccggatgacagtgacgaggagaactgaACCCCAGGCTTTacacTTTATG" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e promoter complement(6727..6732) /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 protein_bind 6747..6768 /label="CAP binding site" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b primer 6799..6819 /label="oTG1156_mCherry-LacI-Rev" /note="sequence: AAGCTTCCATTCGGGAAACCTGTCGtgCCAG" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer 6819..6839 /label="oTG1089_Rev-LacI-NLS-NOSTOP-GA" /note="sequence: AACCTTCCTCTTCTTCTTAGGAGGCTGCATTAATGAATCGGCCA" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer 6819..6840 /label="oTG1084_Rev-LacI-GA" /note="sequence: TCAAACCTTCCTCTtcTTcTTaGgagGCTGCATTAATGAATCGGCCAA" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer 6876..6893 /label="oTG775_pET_BbvCI_insert_seq" /note="sequence: TTCCGCTTCCTCGCTCAC" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 rep_origin complement(7056..7643) /label="ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer 7697..7720 /label="oTG1180_AmpR_seq" /note="sequence: ACGTTAAGGGATTTTGGTCATGAG" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(7783..7813) /label="oTG1177_FRT_AmpR_3’flanking" /note="sequence: CTATACTTTCTAGAGAATAGGAACTTCctgtcagaccaagtttactcatatatacttt" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer complement(7783..7813) /label="oTG1554_pBS_diffusion_substrate_3kb" /note="sequence: ctgtcagaccaagtttactcatatatacttt" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer 7784..7813 /label="oTG1182_E75_AmpR_F" /note="sequence: gactaactgccactcgcagcgcgcagatcgAAGTATATATGAGTAAACTTGGTCTGACAG" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer 7813..7837 /label="oTG1549_pBS_849_adapter" /note="sequence: GCTGTGTCTTATTACACACAGTCGttaccaatgcttaatcagtgaggc" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 CDS complement(join(7814..7956,1..649)) /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 7814..7837 /label="oTG1176_FRT_AmpR_3’" /note="sequence: CTATTCTCTAGAAAGTATAGGAACTTCttaccaatgcttaatcagtgaggc" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer complement(7860..7882) /label="oTG1550_pBS_diffusion_substrate_100bp" /note="sequence: caggcaactatggatgaacgaaa" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 ORIGIN 1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC 61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG 121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA 181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA 241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA 301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA 361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA 421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG 481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG 541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG 601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG 661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC 721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA 781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG 841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT 901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA 961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT 1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA 1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA 1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG 1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA 1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG 1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG 1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC 1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG 1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG 1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG 1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT 1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA 1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT 1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT 1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT 1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA 1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC 2041 TGGGGGGATA CGGGGAAAAG GCGAACGCGT gggtagggga ggcgcttttc ccaaggcagt 2101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc 2161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct 2221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt 2281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca 2341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct 2401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc 2461 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa 2521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc 2581 caAGCTTCAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC 2641 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG 2701 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG 2761 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA 2821 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG 2881 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC 2941 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA 3001 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC 3061 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA 3121 CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC 3181 CCGGTGCCTG AGCGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC 3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC 3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT 3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG 3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AACGCGATCG CTCCGGTGCC 3481 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC 3541 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC 3601 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG 3661 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC 3721 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC 3781 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA 3841 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC 3901 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT 3961 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCCAC CATGggagtg 4021 caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc 4081 gtggtgcact acaccgggat gcttgaagat ggaaagaaat ttgattcctc ccgggacaga 4141 aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg 4201 gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat 4261 ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt cgatgtggag 4321 cttctaaaac tggaaagtgc tggtggtatg gacaaagact gcgaaatgaa gcgcaccacc 4381 ctggatagcc ctctgggcaa gctggaactg tctgggtgcg aacagggcct gcaccgtatc 4441 atcttcctgg gcaaaggaac atctgccgcc gacgccgtgg aagtgcctgc cccagccgcc 4501 gtgctgggcg gaccagagcc actgatgcag gccaccgcct ggctcaacgc ctactttcac 4561 cagcctgagg ccatcgagga gttccctgtg ccagccctgc accacccagt gttccagcag 4621 gagagcttta cccgccaggt gctgtggaaa ctgctgaaag tggtgaagtt cggagaggtc 4681 atcagctaca gccacctggc cgccctggcc ggcaatcccg ccgccaccgc cgccgtgaaa 4741 accgccctga gcggaaatcc cgtgcccatt ctgatcccct gccaccgggt ggtgcagggc 4801 gacctggacg tggggggcta cgagggcggg ctcgccgtga aagagtggct gctggcccac 4861 gagggccaca gactgggcaa gcctgggctg ggtGAGATTT CCGGCagcgg agatggcgct 4921 ggactgatca acggcCGAGC TGATCCAAAA AAGAAGAGAA AGGTAGATCC AAAAAAGAAG 4981 AGAAAGGTAG ATCCAAAAAA GAAGAGAAAG GTAGGATCCA CCGGATCTAG ATAACTGATC 5041 ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC 5101 CCCCTGAACC TGAACGCGGC CGCtggGTCG ACAATCAACC TCTGGATTAC AAAATTTGTG 5161 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT 5221 TAATGCCTTT GTATCATGCG TTAACTAAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA 5281 TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT 5341 GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA ATTGACTCAA ATGATGTCAA 5401 TTAGTCTATC AGAAGCTCAT CTGGTCTCCC TTCCGGGGGA CAAGACATCC CTGTTTAATA 5461 TTTAAACAGC AGTGTTCCCA AACTGGGTTC TTATATCCCT TGCTCTGGTC AACCAGGTTG 5521 CAGGGTTTCC TGTCCTCACA GGAACGAAGT CCCTAAAGAA ACAGTGGCAG CCAGGTTTAG 5581 CCCCGGAATT GACTGGATTC CTTTTTTAGG GCCCATTGGT ATGGCTTTTT CCCCGTATCC 5641 CCCCAGGTGT CTGCAGGCTC AAAGAGCAGC GAGAAGCGTT CAGAGGAAAG CGATCCCGTG 5701 CCACCTTCCC CGTGCCCGGG CTGTCCCCGC ACGCTGCCGG CTCGGGGATG CGGGGGGAGC 5761 GCCGGACCGG AGCGGAGCCC CGGGCGGCTC GCTGCTGCCC CCTAGCGGGG GAGGGACGTA 5821 ATTACATCCC TGGGGGCTTT GGGGGGGGGC TGTCCCTGAT ATCTATAACA AGAAATATAT 5881 ATATAATAAG TTATCACGTA AGTAGAACAT GAAATAACAA TATAATTATC GTATGAGTTA 5941 ATCTTAAAGT CACGTAAAAG ATAATCATGC GTCATTTTGA CTCACGCGGT CGTTATAGTT 6001 CAAAATCAGT GACACTTACC GCATTGACAA GCACGCCTCA CGGGAGCTCC AAGCGGCGAC 6061 TGAGATGTCC TAAATGCACA GCGACGGATT CGCGCTATTT AGAAAGAGAG AGCAATATTT 6121 CAAGAATGCA TGCGTCAATT TTACGCAGAC TATCTTTCTA GGGTTAATCT AGCTGCATCA 6181 GGATCATATC GTCGGGTCTT TTTTCCGGCT CAGTCATCGC CCAAGCTGGC GCTATCTGGG 6241 CATCGGGGAG GAAGAAGCCC GTGCCTTTTC CCGCGAGGTT GAAGCGGCAT GGAAAGAGTT 6301 TGCCGAGGAT GACTGCTGCT GCATTGACGT TGAGCGAAAA CGCACGTTTA CCATGATGAT 6361 TCGGGAAGGT GTGGCCATGC ACGCCTTTAA CGGTGAACTG TTCGTTCAGG CCACCTGGGA 6421 TACCAGTTCG TCGCGGCTTT TCCGGACACA GTTCCGGATG GTCAGCCCGA AGCGCATCAG 6481 CAACCCGAAC AATACCGGCG ACAGCCGGAA CTGCCGTGCC GGTGTGCAGA TTAATGACAG 6541 CGGTGCGGCG CTGGGATATT ACGTCAGCGA GGACGGGTAT CCTGGCTGGA TGCCGCAGAA 6601 ATGGACATGG ATACCCCGTG AGTTACCCGG CGGGCGCGCT TGGCGTAATC ATGGTCATAG 6661 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC 6721 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC 6781 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA 6841 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG 6901 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG 6961 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG 7021 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC 7081 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA 7141 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 7201 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC 7261 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC 7321 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA 7381 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT 7441 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA 7501 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAGAGTT GGTAGCTCTT 7561 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA 7621 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 7681 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 7741 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 7801 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 7861 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 7921 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAG //