https://github.com/satijalab/seurat
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Tip revision: 9b3892961c9e1bf418af3bbb1bc79950adb481d7 authored by Paul Hoffman on 11 June 2021, 19:17:45 UTC
Merge pull request #4608 from satijalab/develop
Tip revision: 9b38929
MapQuery.Rd
% Generated by roxygen2: do not edit by hand
% Please edit documentation in R/integration.R
\name{MapQuery}
\alias{MapQuery}
\title{Map query cells to a reference}
\usage{
MapQuery(
  anchorset,
  query,
  reference,
  refdata = NULL,
  new.reduction.name = NULL,
  reference.reduction = NULL,
  reference.dims = NULL,
  query.dims = NULL,
  reduction.model = NULL,
  transferdata.args = list(),
  integrateembeddings.args = list(),
  projectumap.args = list(),
  verbose = TRUE
)
}
\arguments{
\item{anchorset}{An AnchorSet object}

\item{query}{Query object used in anchorset construction}

\item{reference}{Reference object used in anchorset construction}

\item{refdata}{Data to transfer. This can be specified in one of two ways:
\itemize{
  \item{The reference data itself as either a vector where the names
  correspond to the reference cells, or a matrix, where the column names
  correspond to the reference cells.}
  \item{The name of the metadata field or assay from the reference object
  provided. This requires the reference parameter to be specified. If pulling
  assay data in this manner, it will pull the data from the data slot. To
  transfer data from other slots, please pull the data explicitly with
  \code{\link{GetAssayData}} and provide that matrix here.}
}}

\item{new.reduction.name}{Name for new integrated dimensional reduction.}

\item{reference.reduction}{Name of reduction to use from the reference for
neighbor finding}

\item{reference.dims}{Dimensions (columns) to use from reference}

\item{query.dims}{Dimensions (columns) to use from query}

\item{reduction.model}{\code{DimReduc} object that contains the umap model}

\item{transferdata.args}{A named list of additional arguments to
\code{\link{TransferData}}}

\item{integrateembeddings.args}{A named list of additional arguments to
\code{\link{IntegrateEmbeddings}}}

\item{projectumap.args}{A named list of additional arguments to
\code{\link{ProjectUMAP}}}

\item{verbose}{Print progress bars and output}
}
\value{
Returns a modified query Seurat object containing:

\itemize{
  \item{New Assays corresponding to the features transferred and/or their
  corresponding prediction scores from \code{\link{TransferData}}}
  \item{An integrated reduction from \code{\link{IntegrateEmbeddings}}}
  \item{A projected UMAP reduction of the query cells projected into the
  reference UMAP using \code{\link{ProjectUMAP}}}
}
}
\description{
This is a convenience wrapper function around the following three functions
that are often run together when mapping query data to a reference:
\code{\link{TransferData}}, \code{\link{IntegrateEmbeddings}},
\code{\link{ProjectUMAP}}. Note that by default, the \code{weight.reduction}
parameter for all functions will be set to the dimension reduction method
used in the \code{\link{FindTransferAnchors}} function call used to construct
the anchor object, and the \code{dims} parameter will be the same dimensions
used to find anchors.
}
\concept{integration}
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