https://github.com/dereneaton/ipyrad
Tip revision: 2bbb0d63241745da42544100be2f52f73a013380 authored by Isaac Overcast on 13 April 2017, 12:46:03 UTC
"Updating ipyrad/__init__.py to version - 0.6.14
"Updating ipyrad/__init__.py to version - 0.6.14
Tip revision: 2bbb0d6
__main__.py
#!/usr/bin/env python2
""" the main CLI for calling ipyrad """
from __future__ import print_function, division # Requires Python 2.7+
from ipyrad.core.parallel import register_ipcluster
from ipyrad.assemble.util import IPyradError, IPyradWarningExit, detect_cpus
import pkg_resources
import ipyrad as ip
import argparse
import logging
import atexit
import time
import sys
import os
# pylint: disable=W0212
# pylint: disable=C0301
LOGGER = logging.getLogger(__name__)
def parse_params(args):
""" Parse the params file args, create and return Assembly object."""
## check that params.txt file is correctly formatted.
try:
with open(args.params) as paramsin:
plines = paramsin.readlines()
except IOError as _:
sys.exit(" No params file found")
## check header: big version changes can be distinguished by the header
legacy_version = 0
try:
## try to update the Assembly ...
legacy_version = 1
if not len(plines[0].split()[0]) == 7:
raise IPyradWarningExit("""
Error: file '{}' is not compatible with ipyrad v.{}.
Please create and update a new params file using the -n argument.
For info on which parameters have changed see the changelog:
(http://ipyrad.readthedocs.io/releasenotes.html)
""".format(args.params, ip.__version__))
except IndexError:
raise IPyradWarningExit("""
Error: Params file should not have any empty lines at the top
of the file. Verify there are no blank lines and rerun ipyrad.
Offending file - {}
""".format(args.params))
## update and backup
if legacy_version:
#which version...
#update_to_6()
pass
## make into a dict. Ignore blank lines at the end of file
## Really this will ignore all blank lines
items = [i.split("##")[0].strip() for i in plines[1:] if not i.strip() == ""]
#keys = [i.split("]")[-2][-1] for i in plines[1:]]
#keys = range(len(plines)-1)
keys = ip.Assembly('null', quiet=True).paramsdict.keys()
parsedict = {str(i):j for i, j in zip(keys, items)}
return parsedict
def showstats(parsedict):
""" loads assembly or dies, and print stats to screen """
#project_dir = parsedict['1']
project_dir = parsedict["project_dir"]
if not project_dir:
project_dir = "./"
## Be nice if somebody also puts in the file extension
#assembly_name = parsedict['0']
assembly_name = parsedict["assembly_name"]
my_assembly = os.path.join(project_dir, assembly_name)
## If the project_dir doesn't exist don't even bother trying harder.
if not os.path.isdir(project_dir):
msg = """
Trying to print stats for Assembly ({}) that doesn't exist. You must
first run steps before you can show results.
""".format(project_dir)
sys.exit(msg)
if not assembly_name:
msg = """
Assembly name is not set in params.txt, meaning it was either changed or
erased since the Assembly was started. Please restore the original name.
You can find the name of your Assembly in the "project dir": {}.
""".format(project_dir)
raise IPyradError(msg)
data = ip.load_json(my_assembly, quiet=True)
print("\nSummary stats of Assembly {}".format(data.name) \
+"\n------------------------------------------------")
if not data.stats.empty:
print(data.stats)
print("\n\nFull stats files"\
+"\n------------------------------------------------")
fullcurdir = os.path.realpath(os.path.curdir)
for i in range(1, 8):
#enumerate(sorted(data.stats_files)):
key = "s"+str(i)
try:
val = data.stats_files[key]
val = val.replace(fullcurdir, ".")
print("step {}: {}".format(i, val))
except (KeyError, AttributeError):
print("step {}: None".format(i))
print("\n")
else:
print("No stats to display")
def branch_assembly(args, parsedict):
"""
Load the passed in assembly and create a branch. Copy it
to a new assembly, and also write out the appropriate params.txt
"""
## Get the current assembly
data = getassembly(args, parsedict)
## get arguments to branch command
bargs = args.branch
## get new name, trim off .txt if it was accidentally added
newname = bargs[0]
if newname.endswith(".txt"):
newname = newname[:-4]
## look for subsamples
if len(bargs) > 1:
## are we removing or keeping listed samples?
subsamples = bargs[1:]
## drop the matching samples
if bargs[1] == "-":
## check drop names
fails = [i for i in subsamples[1:] if i not in data.samples.keys()]
if any(fails):
raise IPyradWarningExit("\
\n Failed: unrecognized names requested, check spelling:\n {}"\
.format("\n ".join([i for i in fails])))
print(" dropping {} samples".format(len(subsamples)-1))
subsamples = list(set(data.samples.keys()) - set(subsamples))
## If the arg after the new param name is a file that exists
if os.path.exists(bargs[1]):
new_data = data.branch(newname, infile=bargs[1])
else:
new_data = data.branch(newname, subsamples)
## keeping all samples
else:
new_data = data.branch(newname, None)
print(" creating a new branch called '{}' with {} Samples".\
format(new_data.name, len(new_data.samples)))
print(" writing new params file to {}"\
.format("params-"+new_data.name+".txt\n"))
new_data.write_params("params-"+new_data.name+".txt", force=args.force)
def merge_assemblies(args):
"""
merge all given assemblies into a new assembly. Copies the params
from the first passed in extant assembly. this function is called
with the ipyrad -m flag. You must pass it at least 3 values, the first
is a new assembly name (a new `param-newname.txt` will be created).
The second and third args must be params files for currently existing
assemblies. Any args beyond the third must also be params file for
extant assemblies.
"""
print("\n Merging assemblies: {}".format(args.merge[1:]))
## Make sure there are the right number of args
if len(args.merge) < 3:
sys.exit(_WRONG_NUM_CLI_MERGE)
## Make sure the first arg isn't a params file, i could see someone doing it
newname = args.merge[0]
if os.path.exists(newname) and "params-" in newname:
sys.exit(_WRONG_ORDER_CLI_MERGE)
## Make sure first arg will create a param file that doesn't already exist
if os.path.exists("params-" + newname + ".txt") and not args.force:
sys.exit(_NAME_EXISTS_MERGE.format("params-" + newname + ".txt"))
## Make sure the rest of the args are params files that already exist
assemblies_to_merge = args.merge[1:]
for assembly in assemblies_to_merge:
if not os.path.exists(assembly):
sys.exit(_DOES_NOT_EXIST_MERGE.format(assembly))
## Get assemblies for each of the passed in params files.
## We're recycling some of the machinery for loading assemblies here
assemblies = []
for params_file in args.merge[1:]:
args.params = params_file
parsedict = parse_params(args)
assemblies.append(getassembly(args, parsedict))
## Do the merge
merged_assembly = ip.merge(newname, assemblies)
## Write out the merged assembly params file and report success
merged_assembly.write_params("params-{}.txt".format(newname), force=args.force)
print("\n Merging succeeded. New params file for merged assembly:")
print("\n params-{}.txt\n".format(newname))
def getassembly(args, parsedict):
""" loads assembly or creates a new one and set its params from
parsedict. Does not launch ipcluster.
"""
## Creating an assembly with a full path in the name will "work"
## but it is potentially dangerous, so here we have assembly_name
## and assembly_file, name is used for creating new in cwd, file is
## used for loading existing.
##
## Be nice if the user includes the extension.
#project_dir = ip.core.assembly._expander(parsedict['1'])
#assembly_name = parsedict['0']
project_dir = ip.core.assembly._expander(parsedict['project_dir'])
assembly_name = parsedict['assembly_name']
assembly_file = os.path.join(project_dir, assembly_name)
## Assembly creation will handle error checking on
## the format of the assembly_name
## make sure the working directory exists.
if not os.path.exists(project_dir):
os.mkdir(project_dir)
try:
## If 1 and force then go ahead and create a new assembly
if ('1' in args.steps) and args.force:
data = ip.Assembly(assembly_name)
else:
data = ip.load_json(assembly_file)
except IPyradWarningExit as _:
## if no assembly is found then go ahead and make one
if '1' not in args.steps:
raise IPyradWarningExit(\
" Error: You must first run step 1 on the assembly: {}"\
.format(assembly_file))
else:
## create a new assembly object
data = ip.Assembly(assembly_name)
## for entering some params...
for param in parsedict:
## trap assignment of assembly_name since it is immutable.
if param == "assembly_name":
## Raise error if user tried to change assembly name
if parsedict[param] != data.name:
data.set_params(param, parsedict[param])
else:
## all other params should be handled by set_params
try:
data.set_params(param, parsedict[param])
except IndexError as _:
print(" Malformed params file: {}".format(args.params))
print(" Bad parameter {} - {}".format(param, parsedict[param]))
sys.exit(-1)
return data
def _check_version():
""" Test if there's a newer version and nag the user to upgrade."""
import urllib2
from distutils.version import LooseVersion
header = \
"\n -------------------------------------------------------------"+\
"\n ipyrad [v.{}]".format(ip.__version__)+\
"\n Interactive assembly and analysis of RAD-seq data"+\
"\n -------------------------------------------------------------"
try:
htmldat = urllib2.urlopen("https://anaconda.org/ipyrad/ipyrad").readlines()
curversion = next((x for x in htmldat if "subheader" in x), None).split(">")[1].split("<")[0]
if LooseVersion(ip.__version__) < LooseVersion(curversion):
msg = """
A new version of ipyrad is available (v.{}). To upgrade run:
conda install -c ipyrad ipyrad\n""".format(curversion)
print(header + "\n" + msg)
else:
pass
#print("You are up to date")
except Exception as inst:
## Always fail silently
pass
def parse_command_line():
""" Parse CLI args."""
## create the parser
parser = argparse.ArgumentParser(
formatter_class=argparse.RawDescriptionHelpFormatter,
epilog="""\n
* Example command-line usage:
ipyrad -n data ## create new file called params-data.txt
ipyrad -p params-data.txt ## run ipyrad with settings in params file
ipyrad -p params-data.txt -s 123 ## run only steps 1-3 of assembly.
ipyrad -p params-data.txt -s 3 -f ## run step 3, overwrite existing data.
* HPC parallelization across 32 cores
ipyrad -p params-data.txt -s 3 -c 32 --MPI
* Print results summary
ipyrad -p params-data.txt -r
* Branch/Merging Assemblies
ipyrad -p params-data.txt -b newdata
ipyrad -m newdata params-1.txt params-2.txt [params-3.txt, ...]
* Subsample taxa during branching
ipyrad -p params-data.txt -b newdata taxaKeepList.txt
* Documentation: http://ipyrad.readthedocs.io
""")
#* Preview mode (subsamples data)
# ipyrad -p params-data.txt -s --preview
## add arguments
parser.add_argument('-v', '--version', action='version',
version=str(pkg_resources.get_distribution('ipyrad')))
parser.add_argument('-r', "--results", action='store_true',
help="show results summary for Assembly in params.txt and exit")
parser.add_argument('-f', "--force", action='store_true',
help="force overwrite of existing data")
parser.add_argument('-q', "--quiet", action='store_true',
help="do not print to stderror or stdout.")
parser.add_argument('-d', "--debug", action='store_true',
help="print lots more info to ipyrad_log.txt.")
parser.add_argument('-n', metavar='new', dest="new", type=str,
default=None,
help="create new file 'params-{new}.txt' in current directory")
parser.add_argument('-p', metavar='params', dest="params",
type=str, default=None,
help="path to params file for Assembly: params-{assembly_name}.txt")
parser.add_argument('-b', metavar='branch', dest="branch",
type=str, default=None, nargs="*",
help="create a new branch of the Assembly as params-{branch}.txt")
parser.add_argument('-m', metavar='merge', dest="merge",
default=None, nargs="*",
help="merge all assemblies provided into a new assembly")
parser.add_argument('-s', metavar="steps", dest="steps",
type=str, default=None,
help="Set of assembly steps to perform, e.g., -s 123 (Default=None)")
parser.add_argument("-c", metavar="cores", dest="cores",
type=int, default=0,
help="number of CPU cores to use (Default=0=All)")
parser.add_argument("-t", metavar="threading", dest="threads",
type=int, default=2,
help="tune threading of binaries (Default=2)")
parser.add_argument("--MPI", action='store_true',
help="connect to parallel CPUs across multiple nodes")
parser.add_argument("--preview", action='store_true',
help="run ipyrad in preview mode. Subset the input file so it'll run"\
+ "quickly so you can verify everything is working")
parser.add_argument("--ipcluster", action='store_true',
help="connect to running ipcluster instance with default cluster-id=''")
## if no args then return help message
if len(sys.argv) == 1:
parser.print_help()
sys.exit(1)
## parse args
args = parser.parse_args()
if not any(x in ["params", "new"] for x in vars(args).keys()):
print("Bad arguments: ipyrad command must include at least one of"\
+"`-p` or `-n`\n")
parser.print_help()
sys.exit(1)
return args
def main():
""" main function """
## turn off traceback for the CLI
ip.__interactive__ = 0
## Check for a new version on anaconda
_check_version()
## parse params file input (returns to stdout if --help or --version)
args = parse_command_line()
## Turn the debug output written to ipyrad_log.txt up to 11!
## Clean up the old one first, it's cleaner to do this here than
## at the end (exceptions, etc)
if os.path.exists(ip.__debugflag__):
os.remove(ip.__debugflag__)
if args.debug:
print("\n ** Enabling debug mode ** ")
ip._debug_on()
atexit.register(ip._debug_off)
## create new paramsfile if -n
if args.new:
## Create a tmp assembly, call write_params to make default params.txt
try:
tmpassembly = ip.Assembly(args.new, quiet=True)
tmpassembly.write_params("params-{}.txt".format(args.new),
force=args.force)
except Exception as inst:
print(inst)
sys.exit(2)
print("\n New file 'params-{}.txt' created in {}\n".\
format(args.new, os.path.realpath(os.path.curdir)))
sys.exit(2)
## if params then must provide action argument with it
if args.params:
if not any([args.branch, args.results, args.steps]):
print("""
Must provide action argument along with -p argument for params file.
e.g., ipyrad -p params-test.txt -r ## shows results
e.g., ipyrad -p params-test.txt -s 12 ## runs steps 1 & 2
e.g., ipyrad -p params-test.txt -b newbranch ## branch this assembly
""")
sys.exit(2)
if not args.params:
if any([args.branch, args.results, args.steps]):
print("""
Must provide params file for branching, doing steps, or getting results.
e.g., ipyrad -p params-test.txt -r ## shows results
e.g., ipyrad -p params-test.txt -s 12 ## runs steps 1 & 2
e.g., ipyrad -p params-test.txt -b newbranch ## branch this assembly
""")
## if branching, or merging do not allow steps in same command
## print spacer
if any([args.branch, args.merge]):
args.steps = ""
print("")
## always print the header when doing steps
header = \
"\n -------------------------------------------------------------"+\
"\n ipyrad [v.{}]".format(ip.__version__)+\
"\n Interactive assembly and analysis of RAD-seq data"+\
"\n -------------------------------------------------------------"
## Log the current version. End run around the LOGGER
## so it'll always print regardless of log level.
with open(ip.__debugfile__, 'a') as logfile:
logfile.write(header)
logfile.write("\n Begin run: {}".format(time.strftime("%Y-%m-%d %H:%M")))
logfile.write("\n Using args {}".format(vars(args)))
logfile.write("\n Platform info: {}".format(os.uname()))
## if merging just do the merge and exit
if args.merge:
print(header)
merge_assemblies(args)
sys.exit(1)
## create new Assembly or load existing Assembly, quit if args.results
elif args.params:
parsedict = parse_params(args)
if args.branch:
branch_assembly(args, parsedict)
elif args.steps:
## print header
print(header)
## Only blank the log file if we're actually going to run a new
## assembly. This used to be in __init__, but had the side effect
## of occasionally blanking the log file in an undesirable fashion
## for instance if you run a long assembly and it crashes and
## then you run `-r` and it blanks the log, it's crazymaking.
if os.path.exists(ip.__debugfile__):
if os.path.getsize(ip.__debugfile__) > 50000000:
with open(ip.__debugfile__, 'w') as clear:
clear.write("file reset")
## run Assembly steps
## launch or load assembly with custom profile/pid
data = getassembly(args, parsedict)
## set CLI ipcluster terms
data._ipcluster["cores"] = args.cores if args.cores else detect_cpus()
data._ipcluster["threads"] = args.threads
## if more ipcluster args from command-line then use those
if args.MPI:
data._ipcluster["engines"] = "MPI"
if not args.cores:
raise IPyradWarningExit(_MPI_CORES_ERROR)
else:
data._ipcluster["engines"] = "Local"
## launch a NEW ipcluster instance and register "cluster_id"
## for later destruction, and to avoid conflicts between
## simultaneous ipcluster instances. If a user wanted to use
## an ipcluster instance that is already running instead then
## they have to use the API, or to have set args.ipcluster
if args.ipcluster:
data._ipcluster["cluster_id"] = ""
else:
data = register_ipcluster(data)
## set to print headers
data._headers = 1
## run assembly steps
steps = list(args.steps)
data.run(steps=steps, force=args.force, preview=args.preview, show_cluster=1)
if args.results:
showstats(parsedict)
_MPI_CORES_ERROR = """
If using --MPI you must supply the number of cores with the -c argument.
"""
_WRONG_NUM_CLI_MERGE = """
Error: Attempting to merge assemblies but wrong number of args.
The format for the merging command is:
ipyrad -m new_assembly_name params-1.txt params-2.txt
"""
_WRONG_ORDER_CLI_MERGE = """
Looks like you're trying to pass in a params file that
already exists. The first arg for -m should be the name of
the new assembly (this will create a new params file for you)
"""
_NAME_EXISTS_MERGE = """
New Assembly name already exists: {}
The merge Assembly name must be unique
"""
_DOES_NOT_EXIST_MERGE = """
All arguments after the first for -m need to be params files
for assemblies with Samples that are all in state 1. This
Assembly param file does not exist: {}
"""
if __name__ == "__main__":
main()