https://github.com/lh3/bwa
Tip revision: 139f68fc4c3747813783a488aef2adc86626b01b authored by Heng Li on 22 September 2022, 23:52:12 UTC
Merge pull request #367 from martin-g/github-actions-linux-aarch64
Merge pull request #367 from martin-g/github-actions-linux-aarch64
Tip revision: 139f68f
bwtsw2_aux.c
#include <stdlib.h>
#include <stdio.h>
#include <math.h>
#ifdef HAVE_CONFIG_H
#include "config.h"
#endif
#ifdef HAVE_PTHREAD
#include <pthread.h>
#endif
#include "bntseq.h"
#include "bwt_lite.h"
#include "utils.h"
#include "bwtsw2.h"
#include "kstring.h"
#include "bwa.h"
#include "ksw.h"
#include "kseq.h"
KSEQ_DECLARE(gzFile)
#include "ksort.h"
#define __left_lt(a, b) ((a).end > (b).end)
KSORT_INIT(hit, bsw2hit_t, __left_lt)
#ifdef USE_MALLOC_WRAPPERS
# include "malloc_wrap.h"
#endif
extern unsigned char nst_nt4_table[256];
unsigned char nt_comp_table[256] = {
'N','N','N','N', 'N','N','N','N', 'N','N','N','N', 'N','N','N','N',
'N','N','N','N', 'N','N','N','N', 'N','N','N','N', 'N','N','N','N',
'N','N','N','N', 'N','N','N','N', 'N','N','N','N', 'N','N','N','N',
'N','N','N','N', 'N','N','N','N', 'N','N','N','N', 'N','N','N','N',
'N','T','V','G', 'H','N','N','C', 'D','N','N','M', 'N','K','N','N',
'N','N','Y','S', 'A','N','B','W', 'X','R','N','N', 'N','N','N','N',
'n','t','v','g', 'h','n','n','c', 'd','n','n','m', 'n','k','n','n',
'n','n','y','s', 'a','n','b','w', 'x','r','n','N', 'N','N','N','N',
'N','N','N','N', 'N','N','N','N', 'N','N','N','N', 'N','N','N','N',
'N','N','N','N', 'N','N','N','N', 'N','N','N','N', 'N','N','N','N',
'N','N','N','N', 'N','N','N','N', 'N','N','N','N', 'N','N','N','N',
'N','N','N','N', 'N','N','N','N', 'N','N','N','N', 'N','N','N','N',
'N','N','N','N', 'N','N','N','N', 'N','N','N','N', 'N','N','N','N',
'N','N','N','N', 'N','N','N','N', 'N','N','N','N', 'N','N','N','N',
'N','N','N','N', 'N','N','N','N', 'N','N','N','N', 'N','N','N','N',
'N','N','N','N', 'N','N','N','N', 'N','N','N','N', 'N','N','N','N'
};
extern int bsw2_resolve_duphits(const bntseq_t *bns, const bwt_t *bwt, bwtsw2_t *b, int IS);
extern int bsw2_resolve_query_overlaps(bwtsw2_t *b, float mask_level);
bsw2opt_t *bsw2_init_opt()
{
bsw2opt_t *o = (bsw2opt_t*)calloc(1, sizeof(bsw2opt_t));
o->a = 1; o->b = 3; o->q = 5; o->r = 2; o->t = 30;
o->bw = 50;
o->max_ins = 20000;
o->z = 1; o->is = 3; o->t_seeds = 5; o->hard_clip = 0; o->skip_sw = 0;
o->mask_level = 0.50f; o->coef = 5.5f;
o->qr = o->q + o->r; o->n_threads = 1; o->chunk_size = 10000000;
o->max_chain_gap = 10000;
o->cpy_cmt = 0;
return o;
}
void bsw2_destroy(bwtsw2_t *b)
{
int i;
if (b == 0) return;
if (b->aux)
for (i = 0; i < b->n; ++i) free(b->aux[i].cigar);
free(b->aux); free(b->hits);
free(b);
}
bwtsw2_t *bsw2_dup_no_cigar(const bwtsw2_t *b)
{
bwtsw2_t *p;
p = calloc(1, sizeof(bwtsw2_t));
p->max = p->n = b->n;
if (b->n) {
kroundup32(p->max);
p->hits = calloc(p->max, sizeof(bsw2hit_t));
memcpy(p->hits, b->hits, p->n * sizeof(bsw2hit_t));
}
return p;
}
#define __gen_ap(par, opt) do { \
int i; \
for (i = 0; i < 25; ++i) (par).matrix[i] = -(opt)->b; \
for (i = 0; i < 4; ++i) (par).matrix[i*5+i] = (opt)->a; \
(par).gap_open = (opt)->q; (par).gap_ext = (opt)->r; \
(par).gap_end = (opt)->r; \
(par).row = 5; (par).band_width = opt->bw; \
} while (0)
void bsw2_extend_left(const bsw2opt_t *opt, bwtsw2_t *b, uint8_t *_query, int lq, uint8_t *pac, bwtint_t l_pac, uint8_t *_mem)
{
int i;
bwtint_t k;
uint8_t *target = 0, *query;
int8_t mat[25];
bwa_fill_scmat(opt->a, opt->b, mat);
query = calloc(lq, 1);
// sort according to the descending order of query end
ks_introsort(hit, b->n, b->hits);
target = calloc(((lq + 1) / 2 * opt->a + opt->r) / opt->r + lq, 1);
// reverse _query
for (i = 0; i < lq; ++i) query[lq - i - 1] = _query[i];
// core loop
for (i = 0; i < b->n; ++i) {
bsw2hit_t *p = b->hits + i;
int lt = ((p->beg + 1) / 2 * opt->a + opt->r) / opt->r + lq;
int score, j, qle, tle;
p->n_seeds = 1;
if (p->l || p->k == 0) continue;
for (j = score = 0; j < i; ++j) {
bsw2hit_t *q = b->hits + j;
if (q->beg <= p->beg && q->k <= p->k && q->k + q->len >= p->k + p->len) {
if (q->n_seeds < (1<<13) - 2) ++q->n_seeds;
++score;
}
}
if (score) continue;
if (lt > p->k) lt = p->k;
for (k = p->k - 1, j = 0; k > 0 && j < lt; --k) // FIXME: k=0 not considered!
target[j++] = pac[k>>2] >> (~k&3)*2 & 0x3;
lt = j;
score = ksw_extend(p->beg, &query[lq - p->beg], lt, target, 5, mat, opt->q, opt->r, opt->bw, 0, -1, p->G, &qle, &tle, 0, 0, 0);
if (score > p->G) { // extensible
p->G = score;
p->k -= tle;
p->len += tle;
p->beg -= qle;
}
}
free(query); free(target);
}
void bsw2_extend_rght(const bsw2opt_t *opt, bwtsw2_t *b, uint8_t *query, int lq, uint8_t *pac, bwtint_t l_pac, uint8_t *_mem)
{
int i;
bwtint_t k;
uint8_t *target;
int8_t mat[25];
bwa_fill_scmat(opt->a, opt->b, mat);
target = calloc(((lq + 1) / 2 * opt->a + opt->r) / opt->r + lq, 1);
for (i = 0; i < b->n; ++i) {
bsw2hit_t *p = b->hits + i;
int lt = ((lq - p->beg + 1) / 2 * opt->a + opt->r) / opt->r + lq;
int j, score, qle, tle;
if (p->l) continue;
for (k = p->k, j = 0; k < p->k + lt && k < l_pac; ++k)
target[j++] = pac[k>>2] >> (~k&3)*2 & 0x3;
lt = j;
score = ksw_extend(lq - p->beg, &query[p->beg], lt, target, 5, mat, opt->q, opt->r, opt->bw, 0, -1, 1, &qle, &tle, 0, 0, 0) - 1;
// if (score < p->G) fprintf(stderr, "[bsw2_extend_hits] %d < %d\n", score, p->G);
if (score >= p->G) {
p->G = score;
p->len = tle;
p->end = p->beg + qle;
}
}
free(target);
}
/* generate CIGAR array(s) in b->cigar[] */
static void gen_cigar(const bsw2opt_t *opt, int lq, uint8_t *seq[2], int64_t l_pac, const uint8_t *pac, bwtsw2_t *b, const char *name)
{
int i;
int8_t mat[25];
bwa_fill_scmat(opt->a, opt->b, mat);
for (i = 0; i < b->n; ++i) {
bsw2hit_t *p = b->hits + i;
bsw2aux_t *q = b->aux + i;
uint8_t *query;
int beg, end, score;
if (p->l) continue;
beg = (p->flag & 0x10)? lq - p->end : p->beg;
end = (p->flag & 0x10)? lq - p->beg : p->end;
query = seq[(p->flag & 0x10)? 1 : 0] + beg;
q->cigar = bwa_gen_cigar(mat, opt->q, opt->r, opt->bw, l_pac, pac, end - beg, query, p->k, p->k + p->len, &score, &q->n_cigar, &q->nm);
#if 0
if (name && score != p->G) { // debugging only
int j, glen = 0;
for (j = 0; j < q->n_cigar; ++j)
if ((q->cigar[j]&0xf) == 1 || (q->cigar[j]&0xf) == 2)
glen += q->cigar[j]>>4;
fprintf(stderr, "[E::%s] %s - unequal score: %d != %d; (qlen, aqlen, arlen, glen, bw) = (%d, %d, %d, %d, %d)\n",
__func__, name, score, p->G, lq, end - beg, p->len, glen, opt->bw);
}
#endif
if (q->cigar && (beg != 0 || end < lq)) { // write soft clipping
q->cigar = realloc(q->cigar, 4 * (q->n_cigar + 2));
if (beg != 0) {
memmove(q->cigar + 1, q->cigar, q->n_cigar * 4);
q->cigar[0] = beg<<4 | 4;
++q->n_cigar;
}
if (end < lq) {
q->cigar[q->n_cigar] = (lq - end)<<4 | 4;
++q->n_cigar;
}
}
}
}
/* this is for the debugging purpose only */
void bsw2_debug_hits(const bwtsw2_t *b)
{
int i;
printf("# raw hits: %d\n", b->n);
for (i = 0; i < b->n; ++i) {
bsw2hit_t *p = b->hits + i;
if (p->G > 0)
printf("G=%d, G2=%d, len=%d, [%d,%d), k=%lu, l=%lu, #seeds=%d, is_rev=%d\n", p->G, p->G2, p->len, p->beg, p->end, (long)p->k, (long)p->l, p->n_seeds, p->is_rev);
}
}
static void merge_hits(bwtsw2_t *b[2], int l, int is_reverse)
{
int i;
if (b[0]->n + b[1]->n > b[0]->max) {
b[0]->max = b[0]->n + b[1]->n;
b[0]->hits = realloc(b[0]->hits, b[0]->max * sizeof(bsw2hit_t));
}
for (i = 0; i < b[1]->n; ++i) {
bsw2hit_t *p = b[0]->hits + b[0]->n + i;
*p = b[1]->hits[i];
if (is_reverse) {
int x = p->beg;
p->beg = l - p->end;
p->end = l - x;
p->flag |= 0x10;
}
}
b[0]->n += b[1]->n;
bsw2_destroy(b[1]);
b[1] = 0;
}
/* seq[0] is the forward sequence and seq[1] is the reverse complement. */
static bwtsw2_t *bsw2_aln1_core(const bsw2opt_t *opt, const bntseq_t *bns, uint8_t *pac, const bwt_t *target,
int l, uint8_t *seq[2], bsw2global_t *pool)
{
extern void bsw2_chain_filter(const bsw2opt_t *opt, int len, bwtsw2_t *b[2]);
bwtsw2_t *b[2], **bb[2], **_b, *p;
int k, j;
bwtl_t *query;
query = bwtl_seq2bwtl(l, seq[0]);
_b = bsw2_core(bns, opt, query, target, pool);
bwtl_destroy(query);
for (k = 0; k < 2; ++k) {
bb[k] = calloc(2, sizeof(void*));
bb[k][0] = calloc(1, sizeof(bwtsw2_t));
bb[k][1] = calloc(1, sizeof(bwtsw2_t));
}
for (k = 0; k < 2; ++k) { // separate _b into bb[2] based on the strand
for (j = 0; j < _b[k]->n; ++j) {
bsw2hit_t *q;
p = bb[_b[k]->hits[j].is_rev][k];
if (p->n == p->max) {
p->max = p->max? p->max<<1 : 8;
p->hits = realloc(p->hits, p->max * sizeof(bsw2hit_t));
}
q = &p->hits[p->n++];
*q = _b[k]->hits[j];
if (_b[k]->hits[j].is_rev) {
int x = q->beg;
q->beg = l - q->end;
q->end = l - x;
}
}
}
b[0] = bb[0][1]; b[1] = bb[1][1]; // bb[*][1] are "narrow SA hits"
bsw2_chain_filter(opt, l, b); // NB: only unique seeds are chained
for (k = 0; k < 2; ++k) {
bsw2_extend_left(opt, bb[k][1], seq[k], l, pac, bns->l_pac, pool->aln_mem);
merge_hits(bb[k], l, 0); // bb[k][1] is merged to bb[k][0] here
bsw2_resolve_duphits(0, 0, bb[k][0], 0);
bsw2_extend_rght(opt, bb[k][0], seq[k], l, pac, bns->l_pac, pool->aln_mem);
bsw2_resolve_duphits(0, 0, bb[k][0], 0);
b[k] = bb[k][0];
free(bb[k]);
}
merge_hits(b, l, 1); // again, b[1] is merged to b[0]
bsw2_resolve_query_overlaps(b[0], opt->mask_level);
bsw2_destroy(_b[0]); bsw2_destroy(_b[1]); free(_b);
return b[0];
}
/* set ->flag to records the origin of the hit (to forward bwt or reverse bwt) */
static void flag_fr(bwtsw2_t *b[2])
{
int i, j;
for (i = 0; i < b[0]->n; ++i) {
bsw2hit_t *p = b[0]->hits + i;
p->flag |= 0x10000;
}
for (i = 0; i < b[1]->n; ++i) {
bsw2hit_t *p = b[1]->hits + i;
p->flag |= 0x20000;
}
for (i = 0; i < b[0]->n; ++i) {
bsw2hit_t *p = b[0]->hits + i;
for (j = 0; j < b[1]->n; ++j) {
bsw2hit_t *q = b[1]->hits + j;
if (q->beg == p->beg && q->end == p->end && q->k == p->k && q->len == p->len && q->G == p->G) {
q->flag |= 0x30000; p->flag |= 0x30000;
break;
}
}
}
}
typedef struct {
int n, max;
bsw2seq1_t *seq;
} bsw2seq_t;
static int fix_cigar(const bntseq_t *bns, bsw2hit_t *p, int n_cigar, uint32_t *cigar)
{
// FIXME: this routine does not work if the query bridge three reference sequences
int32_t coor, refl, lq;
int x, y, i, seqid;
bns_cnt_ambi(bns, p->k, p->len, &seqid);
coor = p->k - bns->anns[seqid].offset;
refl = bns->anns[seqid].len;
x = coor; y = 0;
// test if the alignment goes beyond the boundary
for (i = 0; i < n_cigar; ++i) {
int op = cigar[i]&0xf, ln = cigar[i]>>4;
if (op == 1 || op == 4 || op == 5) y += ln;
else if (op == 2) x += ln;
else x += ln, y += ln;
}
lq = y; // length of the query sequence
if (x > refl) { // then fix it
int j, nc, mq[2], nlen[2];
uint32_t *cn;
bwtint_t kk = 0;
nc = mq[0] = mq[1] = nlen[0] = nlen[1] = 0;
cn = calloc(n_cigar + 3, 4);
x = coor; y = 0;
for (i = j = 0; i < n_cigar; ++i) {
int op = cigar[i]&0xf, ln = cigar[i]>>4;
if (op == 4 || op == 5 || op == 1) { // ins or clipping
y += ln;
cn[j++] = cigar[i];
} else if (op == 2) { // del
if (x + ln >= refl && nc == 0) {
cn[j++] = (uint32_t)(lq - y)<<4 | 4;
nc = j;
cn[j++] = (uint32_t)y<<4 | 4;
kk = p->k + (x + ln - refl);
nlen[0] = x - coor;
nlen[1] = p->len - nlen[0] - ln;
} else cn[j++] = cigar[i];
x += ln;
} else if (op == 0) { // match
if (x + ln >= refl && nc == 0) {
// FIXME: not consider a special case where a split right between M and I
cn[j++] = (uint32_t)(refl - x)<<4 | 0; // write M
cn[j++] = (uint32_t)(lq - y - (refl - x))<<4 | 4; // write S
nc = j;
mq[0] += refl - x;
cn[j++] = (uint32_t)(y + (refl - x))<<4 | 4;
if (x + ln - refl) cn[j++] = (uint32_t)(x + ln - refl)<<4 | 0;
mq[1] += x + ln - refl;
kk = bns->anns[seqid].offset + refl;
nlen[0] = refl - coor;
nlen[1] = p->len - nlen[0];
} else {
cn[j++] = cigar[i];
mq[nc?1:0] += ln;
}
x += ln; y += ln;
}
}
if (mq[0] > mq[1]) { // then take the first alignment
n_cigar = nc;
memcpy(cigar, cn, 4 * nc);
p->len = nlen[0];
} else {
p->k = kk; p->len = nlen[1];
n_cigar = j - nc;
memcpy(cigar, cn + nc, 4 * (j - nc));
}
free(cn);
}
return n_cigar;
}
static void write_aux(const bsw2opt_t *opt, const bntseq_t *bns, int qlen, uint8_t *seq[2], const uint8_t *pac, bwtsw2_t *b, const char *name)
{
int i;
// allocate for b->aux
if (b->n<<1 < b->max) {
b->max = b->n;
kroundup32(b->max);
b->hits = realloc(b->hits, b->max * sizeof(bsw2hit_t));
}
b->aux = calloc(b->n, sizeof(bsw2aux_t));
// generate CIGAR
gen_cigar(opt, qlen, seq, bns->l_pac, pac, b, name);
// fix CIGAR, generate mapQ, and write chromosomal position
for (i = 0; i < b->n; ++i) {
bsw2hit_t *p = &b->hits[i];
bsw2aux_t *q = &b->aux[i];
q->flag = p->flag & 0xfe;
q->isize = 0;
if (p->l == 0) { // unique hit
float c = 1.0;
int subo;
// fix out-of-boundary CIGAR
q->n_cigar = fix_cigar(bns, p, q->n_cigar, q->cigar);
// compute mapQ
subo = p->G2 > opt->t? p->G2 : opt->t;
if (p->flag>>16 == 1 || p->flag>>16 == 2) c *= .5;
if (p->n_seeds < 2) c *= .2;
q->qual = (int)(c * (p->G - subo) * (250.0 / p->G + 0.03 / opt->a) + .499);
if (q->qual > 250) q->qual = 250;
if (q->qual < 0) q->qual = 0;
if (p->flag&1) q->qual = 0; // this is a random hit
q->pqual = q->qual; // set the paired qual as qual
// get the chromosomal position
q->nn = bns_cnt_ambi(bns, p->k, p->len, &q->chr);
q->pos = p->k - bns->anns[q->chr].offset;
} else q->qual = 0, q->n_cigar = 0, q->chr = q->pos = -1, q->nn = 0;
}
}
static void update_mate_aux(bwtsw2_t *b, const bwtsw2_t *m)
{
int i;
if (m == 0) return;
// update flag, mchr and mpos
for (i = 0; i < b->n; ++i) {
bsw2aux_t *q = &b->aux[i];
q->flag |= 1; // paired
if (m->n == 0) q->flag |= 8; // mate unmapped
if (m->n == 1) {
q->mchr = m->aux[0].chr;
q->mpos = m->aux[0].pos;
if (m->aux[0].flag&0x10) q->flag |= 0x20; // mate reverse strand
if (q->chr == q->mchr) { // set insert size
if (q->mpos + m->hits[0].len > q->pos)
q->isize = q->mpos + m->hits[0].len - q->pos;
else q->isize = q->mpos - q->pos - b->hits[0].len;
} else q->isize = 0;
} else q->mchr = q->mpos = -1;
}
// update mapping quality
if (b->n == 1 && m->n == 1) {
bsw2hit_t *p = &b->hits[0];
if (p->flag & BSW2_FLAG_MATESW) { // this alignment is found by Smith-Waterman
if (!(p->flag & BSW2_FLAG_TANDEM) && b->aux[0].pqual < 20)
b->aux[0].pqual = 20;
if (b->aux[0].pqual >= m->aux[0].qual) b->aux[0].pqual = m->aux[0].qual;
} else if ((p->flag & 2) && !(m->hits[0].flag & BSW2_FLAG_MATESW)) { // properly paired
if (!(p->flag & BSW2_FLAG_TANDEM)) { // pqual is bounded by [b->aux[0].qual,m->aux[0].qual]
b->aux[0].pqual += 20;
if (b->aux[0].pqual > m->aux[0].qual) b->aux[0].pqual = m->aux[0].qual;
if (b->aux[0].pqual < b->aux[0].qual) b->aux[0].pqual = b->aux[0].qual;
}
}
}
}
/* generate SAM lines for a sequence in ks with alignment stored in
* b. ks->name and ks->seq will be freed and set to NULL in the end. */
static void print_hits(const bntseq_t *bns, const bsw2opt_t *opt, bsw2seq1_t *ks, bwtsw2_t *b, int is_pe, bwtsw2_t *bmate)
{
int i, k;
kstring_t str;
memset(&str, 0, sizeof(kstring_t));
if (b == 0 || b->n == 0) { // no hits
ksprintf(&str, "%s\t4\t*\t0\t0\t*\t*\t0\t0\t", ks->name);
for (i = 0; i < ks->l; ++i) kputc(ks->seq[i], &str);
if (ks->qual) {
kputc('\t', &str);
for (i = 0; i < ks->l; ++i) kputc(ks->qual[i], &str);
} else kputs("\t*", &str);
kputc('\n', &str);
}
for (i = 0; b && i < b->n; ++i) {
bsw2hit_t *p = b->hits + i;
bsw2aux_t *q = b->aux + i;
int j, beg, end, type = 0;
// print mandatory fields before SEQ
if (q->cigar == 0) q->flag |= 0x4;
ksprintf(&str, "%s\t%d", ks->name, q->flag | (opt->multi_2nd && i? 0x100 : 0));
ksprintf(&str, "\t%s\t%ld", q->chr>=0? bns->anns[q->chr].name : "*", (long)q->pos + 1);
if (p->l == 0 && q->cigar) { // not a repetitive hit
ksprintf(&str, "\t%d\t", q->pqual);
for (k = 0; k < q->n_cigar; ++k)
ksprintf(&str, "%d%c", q->cigar[k]>>4, (opt->hard_clip? "MIDNHHP" : "MIDNSHP")[q->cigar[k]&0xf]);
} else ksprintf(&str, "\t0\t*");
if (!is_pe) kputs("\t*\t0\t0\t", &str);
else ksprintf(&str, "\t%s\t%d\t%d\t", q->mchr==q->chr? "=" : (q->mchr<0? "*" : bns->anns[q->mchr].name), q->mpos+1, q->isize);
// get the sequence begin and end
beg = 0; end = ks->l;
if (opt->hard_clip && q->cigar) {
if ((q->cigar[0]&0xf) == 4) beg += q->cigar[0]>>4;
if ((q->cigar[q->n_cigar-1]&0xf) == 4) end -= q->cigar[q->n_cigar-1]>>4;
}
for (j = beg; j < end; ++j) {
if (p->flag&0x10) kputc(nt_comp_table[(int)ks->seq[ks->l - 1 - j]], &str);
else kputc(ks->seq[j], &str);
}
// print base quality if present
if (ks->qual) {
kputc('\t', &str);
for (j = beg; j < end; ++j) {
if (p->flag&0x10) kputc(ks->qual[ks->l - 1 - j], &str);
else kputc(ks->qual[j], &str);
}
} else kputs("\t*", &str);
// print optional tags
ksprintf(&str, "\tAS:i:%d\tXS:i:%d\tXF:i:%d\tXE:i:%d\tNM:i:%d", p->G, p->G2, p->flag>>16, p->n_seeds, q->nm);
if (q->nn) ksprintf(&str, "\tXN:i:%d", q->nn);
if (p->l) ksprintf(&str, "\tXI:i:%d", p->l - p->k + 1);
if (p->flag&BSW2_FLAG_MATESW) type |= 1;
if (p->flag&BSW2_FLAG_TANDEM) type |= 2;
if (type) ksprintf(&str, "\tXT:i:%d", type);
if (opt->cpy_cmt && ks->comment) {
int l = strlen(ks->comment);
if (l >= 6 && ks->comment[2] == ':' && ks->comment[4] == ':') {
kputc('\t', &str); kputs(ks->comment, &str);
}
}
kputc('\n', &str);
}
ks->sam = str.s;
free(ks->seq); ks->seq = 0;
free(ks->qual); ks->qual = 0;
free(ks->name); ks->name = 0;
}
static void update_opt(bsw2opt_t *dst, const bsw2opt_t *src, int qlen)
{
double ll = log(qlen);
int i, k;
*dst = *src;
if (dst->t < ll * dst->coef) dst->t = (int)(ll * dst->coef + .499);
// set band width: the query length sets a boundary on the maximum band width
k = (qlen * dst->a - 2 * dst->q) / (2 * dst->r + dst->a);
i = (qlen * dst->a - dst->a - dst->t) / dst->r;
if (k > i) k = i;
if (k < 1) k = 1; // I do not know if k==0 causes troubles
dst->bw = src->bw < k? src->bw : k;
}
/* Core routine to align reads in _seq. It is separated from
* process_seqs() to realize multi-threading */
static void bsw2_aln_core(bsw2seq_t *_seq, const bsw2opt_t *_opt, const bntseq_t *bns, uint8_t *pac, const bwt_t *target, int is_pe)
{
int x;
bsw2opt_t opt;
bsw2global_t *pool = bsw2_global_init();
bwtsw2_t **buf;
buf = calloc(_seq->n, sizeof(void*));
for (x = 0; x < _seq->n; ++x) {
bsw2seq1_t *p = _seq->seq + x;
uint8_t *seq[2], *rseq[2];
int i, l, k;
bwtsw2_t *b[2];
l = p->l;
update_opt(&opt, _opt, p->l);
if (pool->max_l < l) { // then enlarge working space for aln_extend_core()
int tmp = ((l + 1) / 2 * opt.a + opt.r) / opt.r + l;
pool->max_l = l;
pool->aln_mem = realloc(pool->aln_mem, (tmp + 2) * 24);
}
// set seq[2] and rseq[2]
seq[0] = calloc(l * 4, 1);
seq[1] = seq[0] + l;
rseq[0] = seq[1] + l; rseq[1] = rseq[0] + l;
// convert sequences to 2-bit representation
for (i = k = 0; i < l; ++i) {
int c = nst_nt4_table[(int)p->seq[i]];
if (c >= 4) { c = (int)(drand48() * 4); ++k; } // FIXME: ambiguous bases are not properly handled
seq[0][i] = c;
seq[1][l-1-i] = 3 - c;
rseq[0][l-1-i] = 3 - c;
rseq[1][i] = c;
}
if (l - k < opt.t) { // too few unambiguous bases
buf[x] = calloc(1, sizeof(bwtsw2_t));
free(seq[0]); continue;
}
// alignment
b[0] = bsw2_aln1_core(&opt, bns, pac, target, l, seq, pool);
for (k = 0; k < b[0]->n; ++k)
if (b[0]->hits[k].n_seeds < opt.t_seeds) break;
if (k < b[0]->n) {
b[1] = bsw2_aln1_core(&opt, bns, pac, target, l, rseq, pool);
for (i = 0; i < b[1]->n; ++i) {
bsw2hit_t *p = &b[1]->hits[i];
int x = p->beg;
p->flag ^= 0x10, p->is_rev ^= 1; // flip the strand
p->beg = l - p->end;
p->end = l - x;
}
flag_fr(b);
merge_hits(b, l, 0);
bsw2_resolve_duphits(0, 0, b[0], 0);
bsw2_resolve_query_overlaps(b[0], opt.mask_level);
} else b[1] = 0;
// generate CIGAR and print SAM
buf[x] = bsw2_dup_no_cigar(b[0]);
// free
free(seq[0]);
bsw2_destroy(b[0]);
}
if (is_pe) bsw2_pair(&opt, bns->l_pac, pac, _seq->n, _seq->seq, buf);
for (x = 0; x < _seq->n; ++x) {
bsw2seq1_t *p = _seq->seq + x;
uint8_t *seq[2];
int i;
seq[0] = malloc(p->l * 2); seq[1] = seq[0] + p->l;
for (i = 0; i < p->l; ++i) {
int c = nst_nt4_table[(int)p->seq[i]];
if (c >= 4) c = (int)(drand48() * 4);
seq[0][i] = c;
seq[1][p->l-1-i] = 3 - c;
}
update_opt(&opt, _opt, p->l);
write_aux(&opt, bns, p->l, seq, pac, buf[x], _seq->seq[x].name);
free(seq[0]);
}
for (x = 0; x < _seq->n; ++x) {
if (is_pe) update_mate_aux(buf[x], buf[x^1]);
print_hits(bns, &opt, &_seq->seq[x], buf[x], is_pe, buf[x^1]);
}
for (x = 0; x < _seq->n; ++x) bsw2_destroy(buf[x]);
free(buf);
bsw2_global_destroy(pool);
}
#ifdef HAVE_PTHREAD
typedef struct {
int tid, is_pe;
bsw2seq_t *_seq;
const bsw2opt_t *_opt;
const bntseq_t *bns;
uint8_t *pac;
const bwt_t *target;
} thread_aux_t;
/* another interface to bsw2_aln_core() to facilitate pthread_create() */
static void *worker(void *data)
{
thread_aux_t *p = (thread_aux_t*)data;
bsw2_aln_core(p->_seq, p->_opt, p->bns, p->pac, p->target, p->is_pe);
return 0;
}
#endif
/* process sequences stored in _seq, generate SAM lines for these
* sequences and reset _seq afterwards. */
static void process_seqs(bsw2seq_t *_seq, const bsw2opt_t *opt, const bntseq_t *bns, uint8_t *pac, const bwt_t *target, int is_pe)
{
int i;
is_pe = is_pe? 1 : 0;
#ifdef HAVE_PTHREAD
if (opt->n_threads <= 1) {
bsw2_aln_core(_seq, opt, bns, pac, target, is_pe);
} else {
pthread_t *tid;
pthread_attr_t attr;
thread_aux_t *data;
int j;
pthread_attr_init(&attr);
pthread_attr_setdetachstate(&attr, PTHREAD_CREATE_JOINABLE);
data = (thread_aux_t*)calloc(opt->n_threads, sizeof(thread_aux_t));
tid = (pthread_t*)calloc(opt->n_threads, sizeof(pthread_t));
for (j = 0; j < opt->n_threads; ++j) {
thread_aux_t *p = data + j;
p->tid = j; p->_opt = opt; p->bns = bns; p->is_pe = is_pe;
p->pac = pac; p->target = target;
p->_seq = calloc(1, sizeof(bsw2seq_t));
p->_seq->max = (_seq->n + opt->n_threads - 1) / opt->n_threads + 1;
p->_seq->n = 0;
p->_seq->seq = calloc(p->_seq->max, sizeof(bsw2seq1_t));
}
for (i = 0; i < _seq->n; ++i) { // assign sequences to each thread
bsw2seq_t *p = data[(i>>is_pe)%opt->n_threads]._seq;
p->seq[p->n++] = _seq->seq[i];
}
for (j = 0; j < opt->n_threads; ++j) pthread_create(&tid[j], &attr, worker, &data[j]);
for (j = 0; j < opt->n_threads; ++j) pthread_join(tid[j], 0);
for (j = 0; j < opt->n_threads; ++j) data[j]._seq->n = 0;
for (i = 0; i < _seq->n; ++i) { // copy the result from each thread back
bsw2seq_t *p = data[(i>>is_pe)%opt->n_threads]._seq;
_seq->seq[i] = p->seq[p->n++];
}
for (j = 0; j < opt->n_threads; ++j) {
thread_aux_t *p = data + j;
free(p->_seq->seq);
free(p->_seq);
}
free(data); free(tid);
}
#else
bsw2_aln_core(_seq, opt, bns, pac, target, is_pe);
#endif
// print and reset
for (i = 0; i < _seq->n; ++i) {
bsw2seq1_t *p = _seq->seq + i;
if (p->sam) err_printf("%s", p->sam);
free(p->name); free(p->seq); free(p->qual); free(p->sam);
p->tid = -1; p->l = 0;
p->name = p->seq = p->qual = p->sam = 0;
}
err_fflush(stdout);
_seq->n = 0;
}
void bsw2_aln(const bsw2opt_t *opt, const bntseq_t *bns, bwt_t * const target, const char *fn, const char *fn2)
{
gzFile fp, fp2;
kseq_t *ks, *ks2;
int l, is_pe = 0, i, n;
uint8_t *pac;
bsw2seq_t *_seq;
bseq1_t *bseq;
pac = calloc(bns->l_pac/4+1, 1);
for (l = 0; l < bns->n_seqs; ++l)
err_printf("@SQ\tSN:%s\tLN:%d\n", bns->anns[l].name, bns->anns[l].len);
err_fread_noeof(pac, 1, bns->l_pac/4+1, bns->fp_pac);
fp = xzopen(fn, "r");
ks = kseq_init(fp);
_seq = calloc(1, sizeof(bsw2seq_t));
if (fn2) {
fp2 = xzopen(fn2, "r");
ks2 = kseq_init(fp2);
is_pe = 1;
} else fp2 = 0, ks2 = 0, is_pe = 0;
while ((bseq = bseq_read(opt->chunk_size * opt->n_threads, &n, ks, ks2)) != 0) {
int size = 0;
if (n > _seq->max) {
_seq->max = n;
kroundup32(_seq->max);
_seq->seq = realloc(_seq->seq, _seq->max * sizeof(bsw2seq1_t));
}
_seq->n = n;
for (i = 0; i < n; ++i) {
bseq1_t *b = &bseq[i];
bsw2seq1_t *p = &_seq->seq[i];
p->tid = -1; p->l = b->l_seq;
p->name = b->name; p->seq = b->seq; p->qual = b->qual; p->comment = b->comment; p->sam = 0;
size += p->l;
}
fprintf(stderr, "[bsw2_aln] read %d sequences/pairs (%d bp) ...\n", n, size);
free(bseq);
process_seqs(_seq, opt, bns, pac, target, is_pe);
}
// free
free(pac);
free(_seq->seq); free(_seq);
kseq_destroy(ks);
err_gzclose(fp);
if (fn2) {
kseq_destroy(ks2);
err_gzclose(fp2);
}
}