https://github.com/spatialfruitsalad/volume2position
Tip revision: b773b79b8eb1da46f5d1b5cb82b6b6ecc35aeabb authored by Simon Weis on 13 September 2017, 08:57:01 UTC
fixing a bug with reading in specific tomogram types
fixing a bug with reading in specific tomogram types
Tip revision: b773b79
watershed_segmentation.hpp
//This program is free software: you can redistribute it and/or modify
//it under the terms of the GNU General Public License as published by
//the Free Software Foundation, either version 3 of the License, or
//(at your option) any later version.
//
//This program is distributed in the hope that it will be useful,
//but WITHOUT ANY WARRANTY; without even the implied warranty of
//MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
//GNU General Public License for more details.
//
//You should have received a copy of the GNU General Public License
//along with this program. If not, see <http://www.gnu.org/licenses/>.
//
#ifndef WATERSHEDSEGMENTATION_HPP
#define WATERSHEDSEGMENTATION_HPP
#include "tomogram.hpp"
#include "gauss_filter.hpp"
#include "erodate.hpp"
#include "dilate.hpp"
#include "edm.hpp"
#include "hk.hpp"
#include "common.hpp"
class watershedSegmentation
{
// helper function 1
static int lookup_2 (int x, std::vector<int> & rename)
{
assert (x >= 0);
int ret = rename.at(x);
if (ret < 0)
return lookup_2 (-ret, rename);
else
return ret;
}
// helper function 2
static int find_label (unsigned long nx, unsigned long ny, unsigned long nz, unsigned long i, unsigned long j, unsigned long k, tomogram3d<int>& clustermap, tomogram3d<float>& edm )
{
unsigned long imin = std::max (long(i -1), long(0));
unsigned long imax = std::min (long(i +1), long (nx)-1);
unsigned long jmin = std::max (long(j -1), long(0));
unsigned long jmax = std::min (long(j +1), long (ny)-1);
unsigned long kmin = std::max (long(k -1), long(0));
unsigned long kmax = std::min (long(k +1), long (nz)-1);
float grad = 0.;
long grad_label = 0;
unsigned long ii_s = 0, jj_s = 0, kk_s = 0;
for (unsigned long ii = imin; ii <= imax; ++ii)
for (unsigned long jj = jmin; jj <= jmax; ++jj)
for (unsigned long kk = kmin; kk <= kmax; ++kk)
{
float diff = edm.get(ii,jj,kk) - edm.get(i,j,k);
//float distance = sqrt(pow(float(ii-i),2)+pow(float(jj-j),2)+pow(float(kk-k),2));
float distance = sqrt(float((ii-i)*(ii-i) + (jj-j)*(jj-j) + (kk-k)*(kk-k)));
if (distance == 0) continue;
float slope = diff/distance;
if (slope > grad)
{
grad = slope;
grad_label = clustermap.get(ii,jj,kk);
ii_s = ii;
jj_s = jj;
kk_s = kk;
}
}
if (grad < 0.0001)
return 0;
else if (grad_label == 0)
return find_label(nx,ny,nz,ii_s,jj_s,kk_s, clustermap, edm);
else
return grad_label;
}
public:
// pass this the binary image, put out a labelled image
static void Process (tomogram3d<uint8_t>& binary, tomogram3d<int>& out, float erosion_depth, std::string imagefoldername, int minimumCluster= 50000)
{
std::cout << "start segmentation ... " << std::endl;
int nx = binary.get_sx();
int ny = binary.get_sy();
int nz = binary.get_sz();
std::cout << "calculate edm" << std::endl;
tomogram3d<float> edm;
edm::create_edm(binary, edm);
std::cout << "sqrt edm ..." << std::flush;
for (int z = 0; z<nz; z++)
for (int y = 0; y<ny; y++)
for (int x = 0; x<nx; x++){
float value = sqrt( edm.get(x,y,z) );
edm.set(x,y,z,value);
}
std::cout << " done" << std::endl;
// erodate binary image so particles don't overlap anymore. erosiondepth has to be chosen respectively
tomogram3d<uint8_t> erodated_binary = binary;
Erodate::erodate_binary_sphere(erodated_binary, edm, erosion_depth);
WriteImages(erodated_binary, imagefoldername, "5_erosion", nz);
// filter edm for smoother look
gaussFilter::gauss_filter_mask_5(edm , 5, 0.8);
WriteImages(edm, imagefoldername, "6_edm", nz);
// identify individual clusters
HoshenKopelman::Process(erodated_binary, out);
erodated_binary.clear();
// rename vector for HoshenKopelman
std::vector <int> rename;
rename.clear ();
rename.push_back(0);
// find max
int ellipsoid_number = out.find_max();
std::cout << "ellipsoid_number = " << ellipsoid_number << std::endl;
for (int i = 1; i<= ellipsoid_number; i++)
rename.push_back(i);
// label previously eroded voxels
int init = ellipsoid_number;
for (int k = 0; k != nz; ++k)
for (int j = 0; j != ny; ++j)
for (int i = 0; i != nx; ++i)
if (binary.get(i,j,k) == 1 && out.get(i,j,k) == 0)
{
out.set (i, j, k, init);
rename.push_back(init);
init++;
}
// check for slope in filtered edm
for (int k = 0; k != nz; ++k)
{
for (int j = 0; j != ny; ++j)
for (int i = 0; i != nx; ++i)
{
if (binary.get(i,j,k) == 1 && out.get(i,j,k) > ellipsoid_number)
{
int imin = std::max (i-1, 0);
int imax = std::min (i+1, int (nx-1));
int jmin = std::max (j-1, 0);
int jmax = std::min (j+1, int (ny-1));
int kmin = std::max (k-1, 0);
int kmax = std::min (k+1, int (nz-1));
float grad = 0.;
int label = out.get(i,j,k);
int grad_label = out.get(i,j,k);
for (int ii = imin; ii <= imax; ++ii)
for (int jj = jmin; jj <= jmax; ++jj)
for (int kk = kmin; kk <= kmax; ++kk)
{
float diff = edm.get(ii,jj,kk) - edm.get(i,j,k);
//sweis @Fabian: Why??? pow is so slow
//float distance = sqrt(pow(float(ii-i),2)+pow(float(jj-j),2)+pow(float(kk-k),2));
float distance = sqrt( (ii-i)*(ii-i) + (jj-j)*(jj-j) + (kk-k)*(kk-k));
if (distance == 0) continue;
float slope = diff/distance;
if (slope > grad)
{
grad = slope;
grad_label = out.get(ii,jj,kk);
}
}
if (grad_label != label)
{
rename.at(label) = - grad_label;
}
else
{
rename.at(label) = grad_label;
}
}
}
}
int clustercount = 1;
for (size_t i = 0; i != rename.size (); ++i)
{
if (rename.at(i) > 0)
{
rename.at(i) = clustercount;
clustercount++;
}
}
std::cout << "start checking rename vektor" << std::endl;
int not_added = 0;
for (unsigned int i = 1; i != rename.size (); ++i)
{
if (rename.at(i) == 0)
{
not_added++;
}
}
std::cout << "not_added = " << not_added << std::endl;
std::cout << "ellipsoid_number = " << ellipsoid_number << std::endl;
std::cout << "clustercount = " << clustercount << std::endl;
// resolve all indirections in the rename map
for (int i = 0; i != (int)rename.size (); ++i)
{
if (watershedSegmentation::lookup_2 (i,rename) > ellipsoid_number)
rename.at(i) = 0;
else
rename.at(i) = watershedSegmentation::lookup_2 (i, rename);
}
for (int k = 0; k != nz; ++k)
for (int j = 0; j != ny; ++j)
for (int i = 0; i != nx; ++i)
{
int newvalue = rename.at(out.get(i,j,k));
out.set(i,j,k, newvalue);
}
std::cout << " done" << std::endl;
// count the number of voxels in each cluster
std::vector<int> clustersize (ellipsoid_number + 1);
for (int k = 0; k != nz; ++k)
for (int j = 0; j != ny; ++j)
for (int i = 0; i != nx; ++i)
clustersize.at(out.get(i,j,k))++;
// remove clusters smaller than a certain threshold
for (int k = 0; k != nz; ++k)
for (int j = 0; j != ny; ++j)
for (int i = 0; i != nx; ++i)
{
if (clustersize.at(out.get(i,j,k)) < minimumCluster) out.set(i,j,k,0);
}
erodated_binary = binary;
Dilate::Process(erodated_binary, 3);
edm::create_edm(erodated_binary, edm);
int added_later = 0;
for (int k = 0; k != nz; ++k)
for (int j = 0; j != ny; ++j)
for (int i = 0; i != nx; ++i)
{
if (binary.get(i,j,k) == 1 && out.get(i,j,k) == 0)
{
out.set(i,j,k,find_label(nx, ny, nz, i, j, k, out, edm));
added_later++;
}
}
std::cout << "added_later = " << added_later << std::endl;
edm.clear();
};
};
#endif //SEGMENTATION_HPP