https://github.com/arjunrajlaboratory/RajLabSeqTools
Revision 1f85fbd0bc7114c89c0a475d72e74ac9b14d774c authored by Sydney Shaffer on 13 October 2015, 12:48:21 UTC, committed by Sydney Shaffer on 13 October 2015, 12:48:21 UTC
1 parent 510a48c
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Tip revision: 1f85fbd0bc7114c89c0a475d72e74ac9b14d774c authored by Sydney Shaffer on 13 October 2015, 12:48:21 UTC
made scripts to run bowtie2 on ATAC data
Tip revision: 1f85fbd
unzipAndConcatenateZippedFastq.sh
#!/bin/bash

ZIPFILEDIRECTORY=$1

OUTFASTADIRECTORY=$2

for dirname in $ZIPFIELDIRECTORY/* ; do
    cd $dirname

    INPUT=`ls *001_R1*`
    SAMPLE=${INPUT%%_*}  # Cuts filename string after first '_'

    if [ ! -d $OUTFASTADIRECTORY/raw ]; then
        mkdir $OUTFASTADIRECTORY/raw
    fi

    if [ ! -d $OUTFASTADIRECTORY/raw/$SAMPLE ]; then
        mkdir $OUTFASTADIRECTORY/raw/$SAMPLE
    fi

    for i in *.gz; do
        gunzip -c $i > ${i%.*}
    done

    FASTQR1=${SAMPLE}_R1.fastq
    FASTQR2=${SAMPLE}_R2.fastq

    cat *R1*fastq > $OUTFASTADIRECTORY/raw/$SAMPLE/$FASTQR1
    cat *R2*fastq > $OUTFASTADIRECTORY/raw/$SAMPLE/$FASTQR2

done

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