Revision 9a281c1ef2a81203b77b327c816b315e356cf317 authored by mbrenn3 on 13 August 2015, 19:07:52 UTC, committed by mbrenn3 on 13 August 2015, 19:07:52 UTC
README.md
# 3d-printed-oxygen-control-insert
###A 3D-printable microfluidic device for controlling dissolved gas environment in a 24-well culture plate
<img src="images/insert.JPG" style="width: 400px;"/>
<img src="images/Fig1.png" style="width: 400px;"/>
####The device is printed complete with embedded channels and hose barbs. A 150 micrometer PDMS membrane is adhered to the pillar bottoms to complete the fluidic network and facilitate gas diffusion. Dissolved gas can be controlled in four 6-well units of a 24-well plate.
<img src="images/Fig2.png" style="width: 400px;"/>
####Dissolved oxygen at the cell culture surface can be modulated to near zero or up to 4 discrete levels.
<img src="images/Fig3.png" style="width: 400px;"/>
#### Oxygen levels influence VEGFA expression in A594 cells.
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