https://github.com/dereneaton/ipyrad
Revision 9cd57c72d271a7bab40ecb7203df1862011f6bc7 authored by dereneaton on 04 March 2016, 03:36:30 UTC, committed by dereneaton on 04 March 2016, 03:36:30 UTC
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Tip revision: 9cd57c72d271a7bab40ecb7203df1862011f6bc7 authored by dereneaton on 04 March 2016, 03:36:30 UTC
docs update
Tip revision: 9cd57c7
params-data1.txt
------ ipyrad params file (v.0.1.52)--------------------------------------------
data1                          ## [0] [assembly_name]: Assembly name. Used to name output directories for assembly steps
./                             ## [1] [project_dir]: Project dir (made in curdir if not present)
                               ## [2] [raw_fastq_path]: Location of raw non-demultiplexed fastq files
                               ## [3] [barcodes_path]: Location of barcodes file
                               ## [4] [sorted_fastq_path]: Location of demultiplexed/sorted fastq files
denovo                         ## [5] [assembly_method]: Assembly method (denovo, reference, denovo+reference, denovo-reference)
                               ## [6] [reference_sequence]: Location of reference sequence file
rad                            ## [7] [datatype]: Datatype (see docs): rad, gbs, ddrad, etc.
TGCAG,                         ## [8] [restriction_overhang]: Restriction overhang (cut1,) or (cut1, cut2)
5                              ## [9] [max_low_qual_bases]: Max low quality base calls (Q<20) in a read
33                             ## [10] [phred_Qscore_offset]: phred Q score offset (only alternative=64)
6                              ## [11] [mindepth_statistical]: Min depth for statistical base calling
6                              ## [12] [mindepth_majrule]: Min depth for majority-rule base calling
10000                          ## [13] [maxdepth]: Max cluster depth within samples
0.85                           ## [14] [clust_threshold]: Clustering threshold for de novo assembly
1                              ## [15] [max_barcode_mismatch]: Max number of allowable mismatches in barcodes
0                              ## [16] [filter_adapters]: Filter for adapters/primers (1 or 2=stricter)
35                             ## [17] [filter_min_trim_len]: Min length of reads after adapter trim
2                              ## [18] [max_alleles_consens]: Max alleles per site in consensus sequences
5, 5                           ## [19] [max_Ns_consens]: Max N's (uncalled bases) in consensus (R1, R2)
8, 8                           ## [20] [max_Hs_consens]: Max Hs (heterozygotes) in consensus (R1, R2)
4                              ## [21] [min_samples_locus]: Min # samples per locus for output
100, 100                       ## [22] [max_SNPs_locus]: Max # SNPs per locus (R1, R2)
5, 99                          ## [23] [max_Indels_locus]: Max # of indels per locus (R1, R2)
0.25                           ## [24] [max_shared_Hs_locus]: Max # heterozygous sites per locus (R1, R2)
0, 0                           ## [25] [edit_cutsites]: Edit cut-sites (R1, R2) (see docs)
1, 2, 2, 1                     ## [26] [trim_overhang]: Trim overhang (see docs) (R1>, <R1, R2>, <R2)
*                              ## [27] [output_formats]: Output formats (see docs)
                               ## [28] [pop_assign_file]: Path to population assignment file
                               ## [29] [excludes]: Samples to be excluded from final output files
                               ## [30] [outgroups]: Outgroup individuals. Excluded from final output files
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