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Revision c5b88eb5aa3fa9f6a657d8b078405ce82f7e0e2d authored by Eric Sanford on 09 October 2019, 20:53:45 UTC, committed by Eric Sanford on 09 October 2019, 20:53:45 UTC
Updated STAR version, set default reference genome to hg38, added gene_names from gtf file when there is no HGNC symbol match, made it easier to change the reference genome by defining it in the setEnvironmentVariables.sh script. Modified some snakemake files to make that alternate pipeline almost runnable on the cluster in an interactive node.
1 parent e650685
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  • optionalStepRunFastQC
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  • runFastQC.sh
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runFastQC.sh
#!/bin/bash
# run from within repo

if [ ! -d $EXPERIMENT/analyzed ]; then
    mkdir $EXPERIMENT/analyzed
fi


CURRENTSAMPLENUMBER=1
for fileName in $EXPERIMENT/raw/* ; do
    echo "Working on sample $CURRENTSAMPLENUMBER of experiment $EXPERIMENT"
    SAMPLEID=`basename "$fileName"`

    if [ ! -d $EXPERIMENT/analyzed/$SAMPLEID ]; then
    mkdir $EXPERIMENT/analyzed/$SAMPLEID
	fi

	destinationDir="$EXPERIMENT/analyzed/$SAMPLEID/fastqc/"
	if [ ! -d $destinationDir ]; then
	    mkdir "$destinationDir"
	fi

	inputFileR1="$EXPERIMENT/raw/$SAMPLEID/${SAMPLEID}_R1.fastq.gz"
    inputFileR2="$EXPERIMENT/raw/$SAMPLEID/${SAMPLEID}_R2.fastq.gz"

    fullCmd="fastqc -o $destinationDir $inputFileR1"
    echo "$fullCmd"
    bsub -J "$EXPERIMENT.FastQC.$CURRENTSAMPLENUMBER" -o "$EXPERIMENT/analyzed/$SAMPLEID/log/$(date +%Y-%m-%d_%H-%M).FastQC.bsub.stdout" -e "$EXPERIMENT/analyzed/$SAMPLEID/log/$(date +%Y-%m-%d_%H-%M).FastQC.bsub.stderr" "$fullCmd"
    echo ""

    if [ $PAIRED_OR_SINGLE_END_FRAGMENTS = "paired" ]; then
        fullCmd="fastqc -o $destinationDir $inputFileR2"
        echo "$fullCmd"
        bsub -J "$EXPERIMENT.FastQC.$CURRENTSAMPLENUMBER" -o "$EXPERIMENT/analyzed/$SAMPLEID/log/$(date +%Y-%m-%d_%H-%M).FastQC.bsub.stdout" -e "$EXPERIMENT/analyzed/$SAMPLEID/log/$(date +%Y-%m-%d_%H-%M).FastQC.bsub.stderr" "$fullCmd"
        echo ""
    fi

    CURRENTSAMPLENUMBER=$((CURRENTSAMPLENUMBER+1))
done
echo "Submitted all samples to FastQC"

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