Revision

**f3b486129684a2e5218550ff370c5abb36509967**authored by juliamuellerleile on**13 October 2021, 16:20:03 UTC**, committed by GitHub on**13 October 2021, 16:20:03 UTC****1 parent**9d994b9

analyse_graph.m

```
%% detect and analyse the population spike
function result = analyse(in_matrix, spikeABC_mV, spikeAB_mV, spike_begin, spike_end)
%% preallocate a matrix for the following values: peak B, peak A,
%peak C, spikeABC, spike AB, TIME of peak B
result = -100*ones((size(in_matrix,2)-1),7);
%% define the interval which is to the right of the spike
ROWspBEG = 1;
while in_matrix(ROWspBEG,1) < spike_begin
ROWspBEG = ROWspBEG+1;
end
ROWspEND = ROWspBEG;
while in_matrix(ROWspEND,1 )< spike_end
ROWspEND = ROWspEND+1;
end
%% analyse the spike
for sweep = 2:size(in_matrix,2)
%find peak B
%peak B is compared with 3 values to the left and 4 values to the right, 2 adjacent values can be >= peakB
for rowB = ROWspBEG+3:(ROWspEND-4)
if ((in_matrix(rowB,sweep) <= in_matrix(rowB-1,sweep)) && (in_matrix(rowB,sweep) <= in_matrix(rowB-2,sweep)) && (in_matrix(rowB,sweep) < in_matrix(rowB-3,sweep))) && ((in_matrix(rowB,sweep)<= in_matrix(rowB+1,sweep)) && (in_matrix(rowB,sweep) <= in_matrix(rowB+2,sweep)) && (in_matrix(rowB,sweep) < in_matrix(rowB+3,sweep)) && (in_matrix(rowB,sweep)<in_matrix(rowB+4,sweep)))
peakB = in_matrix(rowB,sweep);
timeB = in_matrix(rowB,1);
%after finding peak B, search for peak A and peak C
peakA = in_matrix(ROWspBEG,sweep);
for rowA = ROWspBEG:(rowB-1)
if in_matrix(rowA,sweep) > peakA
peakA = in_matrix(rowA,sweep);
timeA = in_matrix(rowA,1);
end
end
peakC = in_matrix(rowB+1,sweep);
for rowC = (rowB + 1):ROWspEND
if in_matrix(rowC,sweep) > peakC
peakC = in_matrix(rowC,sweep);
timeC = in_matrix(rowC,1);
end
end
%calculate the value of spikeABC and if it is greater than the given values
%for spikeABC_mV and spikeAB_mV, it is considered a spike and the search
%is finished; if the spikeABC value does not fulfill the conditions, the
%the search continues
spikeABC = (peakA/2 + peakC/2) - peakB;
spikeAB = peakA - peakB;
if (spikeABC >= spikeABC_mV) && (spikeAB >= spikeAB_mV)
result(sweep - 1,1:6) = [peakB, peakA, peakC, 1000*timeB, spikeAB, spikeABC];
figure %show the graph of each trace on keypress, one at a time
hold on
title(sweep - 1);
plot(1000*in_matrix(ROWspBEG:ROWspEND,1),in_matrix(ROWspBEG:ROWspEND,sweep));
plot([1000*timeB 1000*timeA 1000*timeC],[peakB peakA peakC],'rs','LineWidth',1,'MarkerEdgeColor','k','MarkerFaceColor','g','MarkerSize',8);
waitforbuttonpress;
close;
break
end
end
end
end
```

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