{
"cells": [
{
"cell_type": "code",
"execution_count": 1,
"metadata": {
"Collapsed": "false"
},
"outputs": [],
"source": [
"using FileIO\n",
"using Plots\n",
"using Images\n",
"using ImageSegmentation"
]
},
{
"cell_type": "code",
"execution_count": 465,
"metadata": {
"Collapsed": "false"
},
"outputs": [
{
"data": {
"text/plain": [
"export_cell (generic function with 1 method)"
]
},
"execution_count": 465,
"metadata": {},
"output_type": "execute_result"
}
],
"source": [
"function find_nearest(root, next_level, maxdistance)\n",
" nucleus_distance = [sum(abs.(root .- next_level[i])) for i in 1:length(next_level)]\n",
" mindist, mindist_index = findmin(nucleus_distance);\n",
" if mindist < maxdistance\n",
" return mindist_index\n",
" else\n",
" return nothing\n",
" end\n",
"end\n",
"\n",
"function buildtree(root::Int, position)\n",
" tree = []\n",
" height = length(position)\n",
" level = 2\n",
" child = find_nearest(position[1][root], position[level], 100);\n",
" while( child ≠ nothing && level<height)\n",
" push!(tree, child)\n",
" child = find_nearest(position[level][child], position[level+=1], 100)\n",
" #print(\"$level \")\n",
" end\n",
" tree\n",
"end\n",
"\n",
"function generate_path(pos_map)\n",
" all_path = []\n",
" for root in 1:length(pos_map[1])\n",
" tree = buildtree(root, pos_map);\n",
" if length(tree) > 70\n",
" path = [pos_map[i+1][tree[i]] for i in 1:length(tree) ];\n",
" push!(all_path, path)\n",
" end\n",
" end\n",
" all_path;\n",
"end\n",
"\n",
"function export_cell(track, imgs)\n",
" height, width = size(imgs[:,:,1]);\n",
" #Acrop_img = zeros(height, width, length(track));\n",
" crop_img = zeros(700, 700, length(track)*20);\n",
" for t in 1:length(track)\n",
" x, y = Int.(floor.(track[t]));\n",
" xmin, xmax, ymin, ymax = x-349 , x+350, y-349, y+350\n",
" if xmin < 1\n",
" xmax = xmax - xmin + 1\n",
" xmin = 1\n",
" end\n",
" if xmax > 1900\n",
" xmin = xmin - (xmax-1900)\n",
" xmax = 1900\n",
" end\n",
" if ymin < 1\n",
" ymax = ymax - ymin +1\n",
" ymin = 1\n",
" end\n",
" if ymax > 1300\n",
" ymin = ymin - (ymax-1300)\n",
" ymax = 1300\n",
" end\n",
" masked_cell= (data[\"edge\"][:,:,t] .== data[\"edge\"][x, y, t] )\n",
" for i in 1:20\n",
" crop_img[:,:,20*(t-1)+i] =(masked_cell.*raw[:,:,20*(t-1)+i])[xmin:xmax, ymin:ymax]\n",
" end\n",
" end\n",
" crop_img;\n",
"end"
]
},
{
"cell_type": "code",
"execution_count": null,
"metadata": {
"scrolled": true
},
"outputs": [],
"source": [
"raw = load(File(format\"TIFF\", \"../mRNA_confocal_hamamatsu-60X-TIRF/20200316/HE7-11-1-80uw-PWM_1_s2.ome.tiff\"));"
]
},
{
"cell_type": "code",
"execution_count": 356,
"metadata": {
"Collapsed": "false"
},
"outputs": [],
"source": [
"data = load(\"mask4stat.jld\");"
]
},
{
"cell_type": "code",
"execution_count": 357,
"metadata": {
"Collapsed": "false"
},
"outputs": [],
"source": [
"nucleus_pos = [ component_centroids(label_components(data[\"nucleus\"][:,:,i])) for i in 1:size(data[\"nucleus\"])[3] ];"
]
},
{
"cell_type": "code",
"execution_count": 374,
"metadata": {},
"outputs": [],
"source": [
"cells = generate_path(nucleus_pos);"
]
},
{
"cell_type": "code",
"execution_count": 380,
"metadata": {},
"outputs": [
{
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]
},
"execution_count": 380,
"metadata": {},
"output_type": "execute_result"
}
],
"source": [
"plot( xlim=(0,1900), ylim=(0,1900))\n",
"map(i->scatter!(i), cells);\n",
"plot!()"
]
},
{
"cell_type": "code",
"execution_count": 466,
"metadata": {},
"outputs": [],
"source": [
"for i in 1: length(cells)\n",
" save(\"path$i.tiff\", N0f16.(export_cell(cells[i], raw)))\n",
"end"
]
},
{
"cell_type": "code",
"execution_count": 393,
"metadata": {},
"outputs": [],
"source": [
"img_export = N0f16.(export_cell(cells[1], raw));"
]
},
{
"cell_type": "code",
"execution_count": 439,
"metadata": {},
"outputs": [],
"source": [
"test = img_export[:,:,1] .> 0;\n",
"minx = miny = 0;\n",
"maxx = maxy = 0;\n",
"\n",
"for y in 1: size(test)[2]\n",
" if (miny==0) && (sum(test[:, y])>0)\n",
" miny = y;\n",
" end\n",
" if (miny > 0) && (sum(test[:, y])==0)\n",
" maxy = y-1;\n",
" break\n",
" end\n",
"end\n",
"\n",
"for x in 1: size(test)[1]\n",
" if (minx==0) && (sum(test[x, :])>0)\n",
" minx = x;\n",
" end\n",
" if (minx > 0) && (sum(test[x, :])==0)\n",
" maxx = x-1;\n",
" break\n",
" end\n",
"end"
]
},
{
"cell_type": "code",
"execution_count": 447,
"metadata": {},
"outputs": [],
"source": [
"save(\"path1_roi.tiff\", N0f16.(export_cell(cells[1], raw))[minx:maxx, miny:maxy, :])"
]
}
],
"metadata": {
"kernelspec": {
"display_name": "Julia 1.4.0",
"language": "julia",
"name": "julia-1.4"
},
"language_info": {
"file_extension": ".jl",
"mimetype": "application/julia",
"name": "julia",
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}
},
"nbformat": 4,
"nbformat_minor": 4
}
