{
"cells": [
{
"cell_type": "markdown",
"metadata": {},
"source": [
"### Example notebook"
]
},
{
"cell_type": "markdown",
"metadata": {},
"source": [
"this is an example notebook"
]
},
{
"cell_type": "code",
"execution_count": 61,
"metadata": {
"collapsed": false
},
"outputs": [
{
"name": "stdout",
"output_type": "stream",
"text": [
"0.3.6\n"
]
}
],
"source": [
"import ipyrad as ip\n",
"print ip.__version__\n",
"\n",
"import os\n",
"import subprocess\n",
"import glob"
]
},
{
"cell_type": "code",
"execution_count": 62,
"metadata": {
"collapsed": false
},
"outputs": [
{
"name": "stdout",
"output_type": "stream",
"text": [
" loading Assembly: cli2\n",
" from saved path: ~/Documents/ipyrad/tests/cli/cli2.json\n"
]
}
],
"source": [
"data = ip.load_json(\"cli/cli2.json\")"
]
},
{
"cell_type": "code",
"execution_count": 81,
"metadata": {
"collapsed": false
},
"outputs": [
{
"name": "stdout",
"output_type": "stream",
"text": [
"['cat', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3K_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2H_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3I_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3J_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1B_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1A_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1D_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1C_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2E_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2F_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3L_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2G_0.consens.gz']\n",
"['cat', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3K_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2H_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3I_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3J_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1B_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1A_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1D_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1C_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2E_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2F_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3L_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2G_0.consens.gz']\n",
"['cat', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3K_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2H_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3I_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3J_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1B_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1A_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1D_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1C_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2E_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2F_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3L_0.consens.gz', '/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2G_0.consens.gz']\n",
"1 loop, best of 3: 6.72 ms per loop\n"
]
}
],
"source": [
"%%timeit -n 1\n",
"## 1. subprocess with redirect (>)\n",
"\n",
"cmd = ['cat'] + glob.glob(os.path.join(data.dirs.consens, \"*consens.gz\"))\n",
"print cmd\n",
"\n",
"\n",
"with open(\"test_catcons.tmp\", 'w') as output:\n",
" call = subprocess.Popen(cmd, stdout=output)\n",
" call.communicate()\n"
]
},
{
"cell_type": "code",
"execution_count": 67,
"metadata": {
"collapsed": true
},
"outputs": [],
"source": [
"%%bash\n",
"\n",
"subs=\"s/W/A/g;s/w/A/g;s/R/A/g;s/r/A/g;s/M/A/g;s/m/A/g;s/K/T/g;s/k/T/g;s/S/C/g;s/s/C/g;s/Y/C/g;s/y/C/g\"\n",
"\n",
"gunzip -c test_concat.gz | \\\n",
" grep -v \"^>\" - | \\\n",
" sed $subs \\\n",
" > test_haps.fq"
]
},
{
"cell_type": "code",
"execution_count": 87,
"metadata": {
"collapsed": false
},
"outputs": [
{
"data": {
"text/plain": [
"'/>/!s/W/A/g;/>/!s/w/A/g;/>/!s/R/A/g;/>/!s/r/A/g;/>/!s/M/A/g;/>/!s/m/A/g;/>/!s/K/T/g;/>/!s/k/T/g;/>/!s/S/C/g;/>/!s/s/C/g;/>/!s/Y/C/g;/>/!s/y/C/g'"
]
},
"execution_count": 87,
"metadata": {},
"output_type": "execute_result"
}
],
"source": [
"## a string of sed substitutions for temporarily replacing hetero sites \n",
"subs = [\"/>/!s/W/A/g\", \"/>/!s/w/A/g\", \"/>/!s/R/A/g\", \"/>/!s/r/A/g\", \n",
" \"/>/!s/M/A/g\", \"/>/!s/m/A/g\", \"/>/!s/K/T/g\", \"/>/!s/k/T/g\", \n",
" \"/>/!s/S/C/g\", \"/>/!s/s/C/g\", \"/>/!s/Y/C/g\", \"/>/!s/y/C/g\"]\n",
"subs = \";\".join(subs)\n",
"subs"
]
},
{
"cell_type": "code",
"execution_count": 90,
"metadata": {
"collapsed": false
},
"outputs": [],
"source": [
"## impute pseudo-haplo information to avoid mismatch at hetero sites\n",
"## the read data with hetero sites is put back into clustered data later\n",
"\n",
"allcons = \"test_catcons.tmp\" ## this is a gzipped file\n",
"cmd1 = [\"gunzip\", \"-c\", allcons]\n",
"cmd2 = [\"sed\", subs]"
]
},
{
"cell_type": "code",
"execution_count": 92,
"metadata": {
"collapsed": false
},
"outputs": [],
"source": [
"proc1 = subprocess.Popen(cmd1, stdout=subprocess.PIPE)\n",
"allhaps = allcons.replace(\"_catcons.tmp\", \"_cathaps.tmp\")\n",
"with open(allhaps, 'w') as output:\n",
" proc2 = proc2 = subprocess.Popen(cmd2, stdin=proc1.stdout, stdout=output) \n",
" proc2.communicate()\n",
"proc1.stdout.close()"
]
},
{
"cell_type": "code",
"execution_count": 93,
"metadata": {
"collapsed": false
},
"outputs": [
{
"name": "stdout",
"output_type": "stream",
"text": [
"total 19M\r\n",
"-rw-rw-r-- 1 deren 1.3M Jun 12 15:59 test_cathaps.tmp\r\n",
"-rw-rw-r-- 1 deren 20K Jun 12 15:58 Untitled.ipynb\r\n",
"-rw-rw-r-- 1 deren 1.3M Jun 12 15:57 output.txt\r\n",
"-rw-rw-r-- 1 deren 1.3M Jun 12 15:41 input.txt\r\n",
"-rw-rw-r-- 1 deren 372K Jun 12 15:35 test_catcons.tmp\r\n",
"-rw-rw-r-- 1 deren 1.1M Jun 12 15:24 test_haps.fq\r\n",
"-rw-rw-r-- 1 deren 372K Jun 12 15:10 \u001b[0m\u001b[01;31mtest_concat.gz\u001b[0m\r\n",
"drwxrwxr-x 15 deren 4.0K Jun 12 13:02 \u001b[01;34mcli\u001b[0m/\r\n",
"-rw-rw-r-- 1 deren 3.0K Jun 12 13:01 params-cli.txt\r\n",
"-rw-rw-r-- 1 deren 1.4M Jun 11 16:41 ipyrad_log.txt\r\n",
"-rw-rw-r-- 1 deren 3.1K Jun 11 16:26 params-data5.txt\r\n",
"drwxrwxr-x 2 deren 4.0K Jun 11 16:04 \u001b[01;34mipsimdata\u001b[0m/\r\n",
"drwxrwxr-x 7 deren 4.0K Jun 6 14:11 \u001b[01;34mpdtest\u001b[0m/\r\n",
"-rw-rw-r-- 1 deren 3.0K Jun 6 14:00 params-pdtest2.txt\r\n",
"-rw-rw-r-- 1 deren 30K Jun 2 10:46 test.json\r\n",
"drwxrwxr-x 2 deren 4.0K Jun 2 10:46 \u001b[01;34mtest_analysis_svd\u001b[0m/\r\n",
"-rw-rw-r-- 1 deren 1.7K May 27 18:53 Untitled2.ipynb\r\n",
"-rw-rw-r-- 1 deren 33K May 23 12:36 pdtest2.json\r\n",
"-rw-rw-r-- 1 deren 32K May 16 02:40 ipsimdata.ipynb\r\n",
"-rw-rw-r-- 1 deren 3.2K May 16 00:52 params-pdtest.txt\r\n",
"-rw-rw-r-- 1 deren 4.3M May 16 00:19 \u001b[01;31mipsimdata.tar.gz\u001b[0m\r\n",
"-rw-rw-r-- 1 deren 821K May 15 21:52 \u001b[01;31mclitest_R1_.fastq.gz\u001b[0m\r\n",
"-rw-rw-r-- 1 deren 144 May 15 21:52 clitest_barcodes.txt\r\n",
"drwxrwxr-x 2 deren 4.0K May 15 18:49 \u001b[01;34mipsimdata2\u001b[0m/\r\n",
"drwxrwxr-x 2 deren 4.0K May 15 17:58 \u001b[01;34miptutorial\u001b[0m/\r\n",
"drwxrwxr-x 7 deren 4.0K May 14 15:30 \u001b[01;34mcli_backup\u001b[0m/\r\n",
"-rw-rw-r-- 1 deren 3.2K May 14 13:33 params-ron4.txt\r\n",
"-rw-rw-r-- 1 deren 22K May 13 17:42 svd4tet.ipynb\r\n",
"-rw-rw-r-- 1 deren 37K May 13 15:09 recipe-plotting-in-R.ipynb\r\n",
"-rw-rw-r-- 1 deren 30K May 13 14:26 cli_svd.json\r\n",
"drwxrwxr-x 2 deren 4.0K May 13 14:26 \u001b[01;34mcli_svd_analysis_svd\u001b[0m/\r\n",
"-rw-rw-r-- 1 deren 3.3K May 10 19:58 params-sub.txt\r\n",
"-rw-rw-r-- 1 deren 3.3K May 10 19:48 params-new.txt\r\n",
"-rw-rw-r-- 1 deren 191K May 9 15:41 cli_downstream.ipynb\r\n",
"-rw-rw-r-- 1 deren 28K May 7 18:51 snp_positions.html\r\n",
"-rw-rw-r-- 1 deren 28K May 7 18:45 out.html\r\n",
"-rw-rw-r-- 1 deren 18K May 7 18:41 \u001b[01;35mout.svg\u001b[0m\r\n",
"-rw-rw-r-- 1 deren 4.3K May 7 18:38 out.pdf\r\n",
"-rw-rw-r-- 1 deren 37K May 5 13:19 cluster_progress_bar_testing.ipynb\r\n",
"-rw-rw-r-- 1 deren 27K May 5 13:19 structure-cookbook.ipynb\r\n",
"-rw-rw-r-- 1 deren 42K May 4 18:07 iptest.json\r\n",
"drwxrwxr-x 2 deren 4.0K May 4 03:05 \u001b[01;34miptest_outfiles\u001b[0m/\r\n",
"drwxrwxr-x 2 deren 4.0K May 4 03:05 \u001b[01;34miptest_consens\u001b[0m/\r\n",
"drwxrwxr-x 7 deren 4.0K May 4 01:59 \u001b[01;34mron4\u001b[0m/\r\n",
"drwxrwxr-x 2 deren 4.0K May 4 01:29 \u001b[01;34miptest_clust_0.85\u001b[0m/\r\n",
"drwxrwxr-x 2 deren 4.0K May 3 23:03 \u001b[01;34miptest_edits\u001b[0m/\r\n",
"drwxrwxr-x 2 deren 4.0K May 3 22:47 \u001b[01;34miptest_fastqs\u001b[0m/\r\n",
"-rw-rw-r-- 1 deren 3.2K May 3 20:34 params-iptest.txt\r\n",
"drwxrwxr-x 8 deren 4.0K May 3 00:12 \u001b[01;34mipsimdata_backup\u001b[0m/\r\n",
"-rw-rw-r-- 1 deren 3.2K May 3 00:02 params-qron.txt\r\n",
"drwxrwxr-x 3 deren 4.0K May 2 12:49 \u001b[01;34mdata\u001b[0m/\r\n",
"-rw-rw-r-- 1 deren 20K May 1 20:48 faster_step5.ipynb\r\n",
"-rw-rw-r-- 1 deren 156 Apr 29 15:50 mydata.csv\r\n",
"-rw-rw-r-- 1 deren 3.3K Apr 29 15:41 params-ARK90.txt\r\n",
"-rw-rw-r-- 1 deren 3.2K Apr 29 15:19 params-ARK.txt\r\n",
"-rw-rw-r-- 1 deren 9.9K Apr 26 16:20 pairddrad tutorial.ipynb\r\n",
"-rw-rw-r-- 1 deren 3.4M Apr 26 12:24 \u001b[01;31mipyradsims.tar.gz\u001b[0m\r\n",
"drwxrwxr-x 2 deren 4.0K Apr 26 12:16 \u001b[01;34moldipsimdata\u001b[0m/\r\n",
"-rw-rw-r-- 1 deren 558K Apr 25 17:22 Ron update.ipynb\r\n",
"-rw-rw-r-- 1 deren 3.2K Apr 25 12:56 params-Rontest.txt\r\n",
"-rw-rw-r-- 1 deren 22K Apr 25 12:47 Rontest.ipynb\r\n",
"-rw-rw-r-- 1 deren 21K Apr 15 19:17 check6.txt\r\n",
"-rw-rw-r-- 1 deren 25K Apr 6 02:08 Untitled1.ipynb\r\n",
"-rw-rw-r-- 1 deren 3.3K Mar 30 12:09 params-data2.txt\r\n",
"-rw-rw-r-- 1 deren 3.2K Mar 30 12:07 params-data1.txt\r\n",
"-rw-rw-r-- 1 deren 41K Mar 9 01:43 apitest.ipynb\r\n",
"drwxrwxr-x 5 deren 4.0K Mar 8 16:59 \u001b[01;34mpairtest\u001b[0m/\r\n",
"-rw-rw-r-- 1 deren 3.2K Mar 8 16:51 params-merge.txt\r\n",
"-rw-rw-r-- 1 deren 3.2K Mar 8 16:49 params-nomerge.txt\r\n",
"-rw-rw-r-- 1 deren 3.2K Mar 8 16:24 params-base.txt\r\n",
"-rw-rw-r-- 1 deren 609 Mar 5 19:13 RAxML_bipartitionsBranchLabels.test\r\n",
"-rw-rw-r-- 1 deren 591 Mar 5 19:13 RAxML_bipartitions.test\r\n",
"-rw-rw-r-- 1 deren 14K Mar 5 19:13 RAxML_info.test\r\n",
"-rw-rw-r-- 1 deren 568 Mar 5 19:13 RAxML_bestTree.test\r\n",
"-rw-rw-r-- 1 deren 8.0K Mar 5 19:13 RAxML_bootstrap.test\r\n",
"drwxrwxr-x 8 deren 4.0K Mar 5 18:26 \u001b[01;34mapi\u001b[0m/\r\n",
"drwxrwxr-x 7 deren 4.0K Mar 4 13:35 \u001b[01;34mpairddtest\u001b[0m/\r\n",
"-rw-rw-r-- 1 deren 3.2K Mar 4 13:24 params-pairddtest.txt\r\n",
"-rw-rw-r-- 1 deren 34K Feb 19 23:38 numba_test.ipynb\r\n",
"-rw-rw-r-- 1 deren 557K Feb 18 11:22 test_rad.ipynb\r\n",
"-rw-rw-r-- 1 deren 2.6K Feb 11 23:54 Ron-params.txt\r\n",
"-rw-rw-r-- 1 deren 547K Feb 11 10:48 quickguide_API.ipynb\r\n",
"-rw-rw-r-- 1 deren 391K Feb 8 19:17 test_refseq.ipynb\r\n",
"-rw-rw-r-- 1 deren 26K Feb 8 19:17 vsearch_merge_tests.ipynb\r\n",
"-rw-rw-r-- 1 deren 13K Feb 8 19:17 recipe X - renaming Samples.ipynb\r\n",
"-rw-rw-r-- 1 deren 33K Feb 8 19:17 test_pairddrad.ipynb\r\n",
"-rw-rw-r-- 1 deren 58K Feb 8 19:17 test_pairgbs.ipynb\r\n",
"-rw-rw-r-- 1 deren 61K Feb 8 19:17 dstats.ipynb\r\n",
"-rw-rw-r-- 1 deren 27K Feb 8 19:17 h5py_testing.ipynb\r\n",
"-rw-rw-r-- 1 deren 4.8K Feb 8 19:17 ipytesting.ipynb\r\n",
"-rw-rw-r-- 1 deren 6.1K Feb 8 19:17 mergepairs.ipynb\r\n",
"-rw-rw-r-- 1 deren 2.6K Jan 23 17:10 test_pairgbs-params.txt\r\n",
"drwxrwxr-x 3 deren 4.0K Nov 26 2015 \u001b[01;34mmergetest\u001b[0m/\r\n"
]
}
],
"source": [
"ll -th"
]
},
{
"cell_type": "code",
"execution_count": 46,
"metadata": {
"collapsed": false
},
"outputs": [
{
"name": "stdout",
"output_type": "stream",
"text": [
"-rw-rw-r-- 1 deren 31688 Jun 12 13:03 \u001b[0m\u001b[01;31m/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1A_0.consens.gz\u001b[0m\u001b[K\r\n",
"-rw-rw-r-- 1 deren 31655 Jun 12 13:03 \u001b[01;31m/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1B_0.consens.gz\u001b[0m\u001b[K\r\n",
"-rw-rw-r-- 1 deren 31765 Jun 12 13:03 \u001b[01;31m/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1C_0.consens.gz\u001b[0m\u001b[K\r\n",
"-rw-rw-r-- 1 deren 31668 Jun 12 13:03 \u001b[01;31m/home/deren/Documents/ipyrad/tests/cli/cli2_consens/1D_0.consens.gz\u001b[0m\u001b[K\r\n",
"-rw-rw-r-- 1 deren 31715 Jun 12 13:03 \u001b[01;31m/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2E_0.consens.gz\u001b[0m\u001b[K\r\n",
"-rw-rw-r-- 1 deren 31727 Jun 12 13:03 \u001b[01;31m/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2F_0.consens.gz\u001b[0m\u001b[K\r\n",
"-rw-rw-r-- 1 deren 31743 Jun 12 13:03 \u001b[01;31m/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2G_0.consens.gz\u001b[0m\u001b[K\r\n",
"-rw-rw-r-- 1 deren 31724 Jun 12 13:03 \u001b[01;31m/home/deren/Documents/ipyrad/tests/cli/cli2_consens/2H_0.consens.gz\u001b[0m\u001b[K\r\n",
"-rw-rw-r-- 1 deren 31716 Jun 12 13:03 \u001b[01;31m/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3I_0.consens.gz\u001b[0m\u001b[K\r\n",
"-rw-rw-r-- 1 deren 31678 Jun 12 13:03 \u001b[01;31m/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3J_0.consens.gz\u001b[0m\u001b[K\r\n",
"-rw-rw-r-- 1 deren 31680 Jun 12 13:03 \u001b[01;31m/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3K_0.consens.gz\u001b[0m\u001b[K\r\n",
"-rw-rw-r-- 1 deren 31716 Jun 12 13:03 \u001b[01;31m/home/deren/Documents/ipyrad/tests/cli/cli2_consens/3L_0.consens.gz\u001b[0m\u001b[K\r\n",
"-rw-rw-r-- 1 deren 122980 Jun 12 13:07 \u001b[01;31m/home/deren/Documents/ipyrad/tests/cli/cli2_consens/cli2_catclust.gz\u001b[0m\u001b[K\r\n"
]
}
],
"source": [
"ll /home/deren/Documents/ipyrad/tests/cli/cli2_consens/*.gz"
]
},
{
"cell_type": "code",
"execution_count": null,
"metadata": {
"collapsed": true
},
"outputs": [],
"source": [
"%%timeit -n 10\n",
"## 1. subprocess with redirect (>)\n",
"\n",
"\n",
"cmd = ['cat', os.path.join(data.dirs.consens, \"*.gz\")]\n",
"\n",
"with gzip.open(\"test_concat.gz\")\n",
"call = subprocess.Popen(cmd, stdout=)\n",
"call.wait()"
]
},
{
"cell_type": "code",
"execution_count": null,
"metadata": {
"collapsed": true
},
"outputs": [],
"source": []
},
{
"cell_type": "code",
"execution_count": 3,
"metadata": {
"collapsed": false
},
"outputs": [
{
"name": "stdout",
"output_type": "stream",
"text": [
" 0 assembly_name test \n",
" 1 project_dir ./ \n",
" 2 raw_fastq_path \n",
" 3 barcodes_path \n",
" 4 sorted_fastq_path \n",
" 5 assembly_method denovo \n",
" 6 reference_sequence \n",
" 7 datatype rad \n",
" 8 restriction_overhang ('TGCAG', '') \n",
" 9 max_low_qual_bases 5 \n",
" 10 phred_Qscore_offset 33 \n",
" 11 mindepth_statistical 6 \n",
" 12 mindepth_majrule 6 \n",
" 13 maxdepth 10000 \n",
" 14 clust_threshold 0.85 \n",
" 15 max_barcode_mismatch 1 \n",
" 16 filter_adapters 0 \n",
" 17 filter_min_trim_len 35 \n",
" 18 max_alleles_consens 2 \n",
" 19 max_Ns_consens (5, 5) \n",
" 20 max_Hs_consens (8, 8) \n",
" 21 min_samples_locus 4 \n",
" 22 max_SNPs_locus (100, 100) \n",
" 23 max_Indels_locus (5, 99) \n",
" 24 max_shared_Hs_locus 0.25 \n",
" 25 edit_cutsites (0, 0) \n",
" 26 trim_overhang (1, 2, 2, 1) \n",
" 27 output_formats * \n",
" 28 pop_assign_file \n",
" 29 excludes \n",
" 30 outgroups \n"
]
}
],
"source": [
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