https://gitlab.com/tgwgraham/papa_paper_plasmids
Tip revision: 984803582c0023a9fe975ec6d41be3d723c2737b authored by Thomas Graham on 08 July 2022, 01:06:31 UTC
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Tip revision: 9848035
ptg802-ef1alpha-3xflag-halo-snapf-60mer-t2a-p2a-snapf.gb
LOCUS pTG802_EF1alpha-3xFlag- 9894 bp ds-DNA circular 13-JUL-2021
DEFINITION .
KEYWORDS "creator:Gina Dailey - Darzacq/Tjian Lab" "marker:AmpR, PuroR"
FEATURES Location/Qualifiers
primer complement(108..126)
/label="oTG1552_pBS_diffusion_substrate_300bp"
/note="sequence: ctctagcttcccggcaaca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS complement(650..718)
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter complement(719..823)
/label="AmpR promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(802..823)
/label="oTG1179_FRT_AmpR_5’"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCcgcggaacccctatttgtttat"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(821..839)
/label="oTG1553_pBS_diffusion_substrate_1kb"
/note="sequence: ttttcggggaaatgtgcgc"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
rep_origin complement(849..1303)
/label="f1 ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 1441..1460
/label="oTG1336_M13F_extended"
/note="sequence: GTTGTAAAACGACGGCCAGT"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind 1443..1460
/label="M13-fwd"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 1444..1460
/label="M13 fwd"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
misc_feature 1590..1602
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 1637..1652
/label="IR (inverted repeat) truncated?"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
promoter 2071..2570
/label="PGK promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS 2592..3191
/label="PuroR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
polyA_signal 3224..3448
/label="bGH poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 3991..4010
/label="oTG1576_3xFlag-Halo_upstream_F"
/note="sequence: CCGGCGCCTACTCTAGCCAC"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS 4012..4014
/label="ATG"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 4012..6951
/label="Translation 4012-6951"
CDS 4015..4080
/label="3xFLAG"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer complement(4057..4081)
/label="oTG1617_Flag_RT3_R"
/note="sequence: CTAGCgccctattgcttgtcatcgtcatccttgtaatc"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4057..4081)
/label="oTG1613_Flag_RT1_R"
/note="sequence: CTAGCttgctattgcttgtcatcgtcatccttgtaatc"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4057..4081)
/label="oTG1615_Flag_RT2_R"
/note="sequence: CTAGCttgctagtgcttgtcatcgtcatccttgtaatc"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4089..4114
/label="oTG945_60xHalo_F"
/note="sequence: CCGGCTCCGGCAGCGGTAGCATGGCAGAAATCGGTACTGGCTTTC"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 4090..4110
/label="oTG778_5'Halo"
/note="sequence: ATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 4090..4110
/label="oTG898_H10_Halo_F"
/note="sequence: TTCTCACCATCACCATCACCACCACCACCACCATATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 4090..4110
/label="oTG1573_GSGSKCK_Halo_F"
/note="sequence: GGTAGTGGTAGTAAGTGTAAAATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer 4090..4110
/label="oTG1582_SNAPf_linker_Halo_F"
/note="sequence: TGGGCAAGCCTGGGCTGGGTggtagtggtaccggttctATGGCAGAAATCGGTACTGGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 4090..4980
/label="HaloTag®"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 4093..4113
/label="oTG1144_inVitro_60xHalo_F"
/note="sequence: GGCTCCGGCAGCGGTAGCGCAGAAATCGGTACTGGCTTT"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer 4096..4118
/label="oTG995_Halo_linker_F"
/note="sequence: GATCTGGAGGTTCAGGAGGCAGCGGTGGAAGTGAAATCGGTACTGGCTTTCCATT"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer 4096..4118
/label="oTG1280_60mer_linker_Halo_F"
/note="sequence: GGAGTGGTAGCGGCAGTATTAAGgaaatcggtactggctttccatt"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(4101..4120)
/label="oTG1172_Halo_int_R2"
/note="sequence: CGAATGGAAAGCCAGTACCG"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(4106..4125)
/label="oTG1214_Halo_int_R_75"
/note="sequence: GGGGTCGAATGGAAAGCCAG"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(4129..4148)
/label="oTG1096_Halo_int_R"
/note="sequence: TCGCCCAGGACTTCCACATA"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(4150..4169)
/label="oTG1173_Halo_int_R3"
/note="sequence: ACATCGACGTAGTGCATGCG"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 4311..4332
/label="oTG1513_Halo_qPCR_F1"
/note="sequence: ACCAGACCTGGGTTATTTCTTC"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer 4451..4470
/label="oTG1208_Halo_int_F"
/note="sequence: AGCGCGTCAAAGGTATTGCA"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(4471..4490)
/label="oTG1514_Halo_qPCR_R1"
/note="sequence: GGGCGGATGAACTCCATAAA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(4565..4587)
/label="oTG1674_Halo_V167C_R"
/note="sequence: GTTCTGATCGATGATCAGCTTGC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4565..4587)
/label="oTG1691_Halo_V167C_R"
/note="sequence: GTTCTGATCGATGATCAGCTTGC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4574..4599)
/label="oTG1676_Halo_G171C_R"
/note="sequence: CTCGATAAAAACGTTCTGATCGATGA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4574..4599)
/label="oTG1693_Halo_G171C_R"
/note="sequence: CTCGATAAAAACGTTCTGATCGATGA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4590..4610
/label="oTG1673_Halo_V167C_F"
/note="sequence: tgtTTTATCGAGGGTACGCTGCC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4590..4610
/label="oTG1690_Halo_V167C_F"
/note="sequence: tgtTTTATCGAGGGTACGCTGCC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4592..4611)
/label="oTG1678_Halo_M175C_R"
/note="sequence: CGGCAGCGTACCCTCGATAA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4592..4611)
/label="oTG1695_Halo_M175C_R"
/note="sequence: CGGCAGCGTACCCTCGATAA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4601..4620
/label="oTG1675_Halo_G171C_F"
/note="sequence: tgtACGCTGCCGATGGGTGTC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4601..4620
/label="oTG1692_Halo_G171C_F"
/note="sequence: tgtACGCTGCCGATGGGTGTC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4615..4632
/label="oTG1694_Halo_M175C_F"
/note="sequence: tgtGGTGTCGTCCGCCCGCTG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 4615..4632
/label="oTG1677_Halo_M175C_F"
/note="sequence: tgtGGTGTCGTCCGCCCGCTG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(4629..4650)
/label="oTG1207_Halo_int_R"
/note="sequence: GTCCATCTCGACTTCAGTCAGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 4636..4657
/label="oTG1515_Halo_qPCR_F2"
/note="sequence: GAAGTCGAGATGGACCATTACC"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer complement(4758..4777)
/label="oTG1516_Halo_qPCR_R2"
/note="sequence: GCAGCCAGTCCATGTATTCT"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 4871..4890
/label="oTG1095_Halo_int_F"
/note="sequence: ACTGCAAGGCTGTGGACATC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 4898..4920
/label="oTG979_3'_Halo_seq"
/note="sequence: GTCTGAATCTGCTGCAAGAAGAC"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer complement(4948..4965)
/label="oTG1312_Halo_tubUTR_R"
/note="sequence: cgcgacgcttagtgtgccgcTTACGTCGACAGCCAGCGCGC"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(4960..4979)
/label="oTG1261_ParB2_Halo_stop_R"
/note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(4960..4979)
/label="oTG1261_ParB2_Halo_stop_R"
/note="sequence: AGGTTCCTTCACAAAGATCCTCTAGAttaACCGGAAATCTCGAGCGTCGA"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(4961..4980)
/label="oTG793_3'Halo_R"
/note="sequence: GCCGGAAATCTCGAGCGTCG"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer complement(4961..4980)
/label="oTG779_Halo-stop_pETSUMO"
/note="sequence: GCTCCAATTGGGTACCCATGGAGCCGGAAATCTCGAGCGTCG"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer complement(4961..4982)
/label="oTG1583_Halo_noNLS_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCgccggaaatctcgagcgtcg"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 4972..4991
/label="Gibson overhang"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 4972..4991
/label="oTG1618_SNAP-3xNLS_gBlock_F"
/note="sequence: ATTTCCGGCggtagtggtac"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 4999..5544
/label="SNAPf"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
CDS 4999..5544
/label="Translation 4999-5544"
primer 5550..5569
/label="oTG1683_3xNLS-P2A-T2A_KpnI_F"
/note="sequence: atttccggcggtagtggtaccTCAGGAAGTGGAGATGGCG"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5577..5626
/label="oTG1620_HaloSNAP60mer-T2AP2A-SNAP_F"
/note="sequence: GATCAATGGACGGGCAGATgctagcATGAAAATGGAAGAGCTGTTCAAGA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 5601..5626
/label="oTG909_60mer_pcDNA_F"
/note="sequence: CCACTAGTCCAGTGTGGTGGCCACCATGAAAATGGAAGAGCTGTTCAAGA"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer 5601..5626
/label="oTG1622_HaloSNAP3xN-TP2A-60merSNAP_F"
/note="sequence: gaaaatcccggcccaGgtaccATGAAAATGGAAGAGCTGTTCAAGA"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 5602..6216
/label="60mer"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer 5605..5626
/label="oTG936_60xGFP_F"
/note="sequence: AAAATGGAAGAGCTGTTCAAGA"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 5605..5630
/label="oTG977_Linker_ctGFP_F"
/note="sequence: GTTCAGGAGGCAGCGGTGGAAGTAAAATGGAAGAGCTGTTCAAGAAACA"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer complement(6198..6216)
/label="oTG1621_HaloSNAP60mer-T2AP2A-SNAP_R"
/note="sequence: ttgtggcgccgctgcccatatgTTCGGTGCAGCCACGAATC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(6198..6216)
/label="oTG1623_HaloSNAP60mer-TP2A-SNAP_R"
/note="sequence: tccacttccgccaccggtaccTTCGGTGCAGCCACGAATC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(6198..6218)
/label="oTG1623_HaloSNAP60mer-TP2A-SNAP_R"
/note="sequence: tccacttccgccaccggtaccTTCGGTGCAGCCACGAATC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(6198..6238)
/label="oTG1621_HaloSNAP60mer-T2AP2A-SNAP_R"
/note="sequence: ttgtggcgccgctgcccatatgTTCGGTGCAGCCACGAATC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 6216..6234
/label="oTG1725_SNAPf-3xNLS_P2A_F"
/note="sequence: AGAAAGGTAGGATCCACCGGAcatatgggcagcggcgcc"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer 6223..6240
/label="oTG1684_P2A-T2A_KpnI_F"
/note="sequence: atttccggcggtagtggtaccGGCAGCGGCGCCACAAAC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 6223..6288
/label="P2A"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 6307..6369
/label="T2A"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
primer complement(6352..6378)
/label="oTG1685_P2A-T2A_KpnI_R"
/note="sequence: tttgtccatagaaccggtaccTGGGCCGGGATTTTCCTC"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(6358..6375)
/label="oTG1726_T2A-KpnI_R"
/note="sequence: ggtacctgggccgggatt"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(6398..6420)
/label="oTG1619_SNAP-3xNLS_gBlock_R"
/note="sequence: gcagtctttgtccatagaacctg"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
misc_feature 6400..6420
/label="Gibson overhang"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 6400..6427
/label="oTG1577_Halo_linker_SNAPf_F"
/note="sequence: CGACGCTCGAGATTTCCGGCggtagtggtaccggttctATGGACAAAGACTGCGAAATGA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 6406..6428
/label="oTG1562_FKBP-SNAPf_F2"
/note="sequence: atggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 6406..6428
/label="oTG1580_Kozak_SNAPf_F"
/note="sequence: CCGGCGCCTACTCTAGCCACCatggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
CDS 6406..6951
/label="SNAPf"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
gene 6406..6951
/label="SNAP26f"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 6406..6951
/label="Translation 6406-6951"
source 6406..6951
/label="source"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(6493..6513)
/label="oTG1722_SNAPf_v2_int_R"
/note="sequence: tttgcccaggaagatgatacg"
/ApEinfo_revcolor=#f7977a
/ApEinfo_fwdcolor=#f7977a
primer complement(6932..6951)
/label="oTG1581_SNAPf_3’_R"
/note="sequence: acccagcccaggcttgccca"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(6932..6951)
/label="oTG1563_FKBP-SNAPf_R2"
/note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(6932..6952)
/label="oTG1563_FKBP-SNAPf_R2"
/note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(6932..6952)
/label="oTG1578_SNAPf_noNLS_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCacccagcccaggcttgccca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(6932..6971)
/label="oTG1563_FKBP-SNAPf_R2"
/note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(6932..6985)
/label="oTG1578_SNAPf_noNLS_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCacccagcccaggcttgccca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 6952..6974
/label="oTG967_pHalo-Stop_F"
/note="sequence: TAGGATCCACCGGATCTAGATAACT"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS 6952..6976
/label="Translation 6952-6976"
primer complement(6957..6985)
/label="oTG1579_3xNLS_downstream_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTG"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 6966..6976
/label="stop codons"
/ApEinfo_revcolor=#ff0000
/ApEinfo_fwdcolor=#ff0000
misc_feature 7053..7053
/label="add_before"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 7053..7070
/label="MCS"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
polyA_signal 7186..7307
/label="SV40 poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(7279..7307)
/label="oTG885_SV40_pA_F"
/note="sequence: TAAGATACATTGATGAGTTTGGACAAACC"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature complement(8067..8085)
/label="IR (inverted repeat)"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
misc_feature complement(8089..8101)
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind complement(8592..8608)
/label="M13 rev"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
protein_bind 8616..8632
/label="lac operator"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
promoter complement(8640..8646)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(8647..8664)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(8658..8679)
/label="oTG980_Vector-Fwd-GA"
/note="sequence: ccggatgacagtgacgaggagaactgaACCCCAGGCTTTacacTTTATG"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
promoter complement(8665..8670)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
protein_bind 8685..8706
/label="CAP binding site"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
primer 8737..8757
/label="oTG1156_mCherry-LacI-Rev"
/note="sequence: AAGCTTCCATTCGGGAAACCTGTCGtgCCAG"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 8757..8777
/label="oTG1089_Rev-LacI-NLS-NOSTOP-GA"
/note="sequence: AACCTTCCTCTTCTTCTTAGGAGGCTGCATTAATGAATCGGCCA"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 8757..8778
/label="oTG1084_Rev-LacI-GA"
/note="sequence: TCAAACCTTCCTCTtcTTcTTaGgagGCTGCATTAATGAATCGGCCAA"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 8814..8831
/label="oTG775_pET_BbvCI_insert_seq"
/note="sequence: TTCCGCTTCCTCGCTCAC"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
rep_origin complement(8994..9581)
/label="ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 9635..9658
/label="oTG1180_AmpR_seq"
/note="sequence: ACGTTAAGGGATTTTGGTCATGAG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(9721..9751)
/label="oTG1177_FRT_AmpR_3’flanking"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer complement(9721..9751)
/label="oTG1554_pBS_diffusion_substrate_3kb"
/note="sequence: ctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 9722..9751
/label="oTG1182_E75_AmpR_F"
/note="sequence: gactaactgccactcgcagcgcgcagatcgAAGTATATATGAGTAAACTTGGTCTGACAG"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer 9751..9775
/label="oTG1549_pBS_849_adapter"
/note="sequence: GCTGTGTCTTATTACACACAGTCGttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
CDS complement(join(9752..9894,1..649))
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 9752..9775
/label="oTG1176_FRT_AmpR_3’"
/note="sequence: CTATTCTCTAGAAAGTATAGGAACTTCttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(9798..9820)
/label="oTG1550_pBS_diffusion_substrate_100bp"
/note="sequence: caggcaactatggatgaacgaaa"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC
61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG
121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA
181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA
241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA
301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA
361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA
421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG
481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG
541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG
601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG
661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC
721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT
901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA
961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA
1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA
1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG
1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA
1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG
1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG
1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC
1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG
1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG
1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG
1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT
1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA
1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT
1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT
1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT
1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA
1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC
2041 TGGGGGGATA CGGGGAAAAG GCGAACGCGT gggtagggga ggcgcttttc ccaaggcagt
2101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
2161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
2221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
2281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
2341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
2401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
2461 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
2521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
2581 caAGCTTCAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC
2641 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG
2701 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG
2761 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA
2821 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG
2881 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC
2941 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA
3001 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC
3061 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA
3121 CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC
3181 CCGGTGCCTG AGCGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AACGCGATCG CTCCGGTGCC
3481 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC
3541 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC
3601 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG
3661 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC
3721 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC
3781 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA
3841 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC
3901 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT
3961 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCCAC CATGGACTAC
4021 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGACAAG
4081 CTTGGCTCCA TGGCAGAAAT CGGTACTGGC TTTCCATTCG ACCCCCATTA TGTGGAAGTC
4141 CTGGGCGAGC GCATGCACTA CGTCGATGTT GGTCCGCGCG ATGGCACCCC TGTGCTGTTC
4201 CTGCACGGTA ACCCGACCTC CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG
4261 ACCCATCGCT GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA ACCAGACCTG
4321 GGTTATTTCT TCGACGACCA CGTCCGCTTC ATGGATGCCT TCATCGAAGC CCTGGGTCTG
4381 GAAGAGGTCG TCCTGGTCAT TCACGACTGG GGCTCCGCTC TGGGTTTCCA CTGGGCCAAG
4441 CGCAATCCAG AGCGCGTCAA AGGTATTGCA TTTATGGAGT TCATCCGCCC TATCCCGACC
4501 TGGGACGAAT GGCCAGAATT TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC
4561 GGCCGCAAGC TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC GATGGGTGTC
4621 GTCCGCCCGC TGACTGAAGT CGAGATGGAC CATTACCGCG AGCCGTTCCT GAATCCTGTT
4681 GACCGCGAGC CACTGTGGCG CTTCCCAAAC GAGCTGCCAA TCGCCGGTGA GCCAGCGAAC
4741 ATCGTCGCGC TGGTCGAAGA ATACATGGAC TGGCTGCACC AGTCCCCTGT CCCGAAGCTG
4801 CTGTTCTGGG GCACCCCAGG CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA
4861 AGCCTGCCTA ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT GCAAGAAGAC
4921 AACCCGGACC TGATCGGCAG CGAGATCGCG CGCTGGCTGT CGACGCTCGA GATTTCCGGC
4981 ggtagtggta caggttctAT GGATAAAGAC TGTGAGATGA AGCGGACAAC CCTTGATTCC
5041 CCTCTGGGGA AACTGGAGCT CAGTGGGTGC GAACAGGGGC TGCACAGAAT TATATTTCTT
5101 GGGAAGGGCA CGTCTGCTGC AGATGCTGTC GAGGTCCCAG CCCCGGCTGC CGTGCTTGGT
5161 GGACCAGAGC CTCTCATGCA AGCTACTGCT TGGCTGAACG CGTATTTCCA CCAACCTGAA
5221 GCGATAGAAG AGTTCCCTGT TCCTGCTCTG CACCACCCGG TTTTCCAACA GGAATCTTTC
5281 ACGAGACAAG TATTGTGGAA ACTTCTCAAA GTGGTCAAAT TCGGTGAAGT AATATCCTAT
5341 TCACACCTGG CAGCTCTCGC CGGGAACCCG GCGGCAACCG CTGCGGTTAA AACTGCACTC
5401 TCTGGTAATC CTGTACCAAT ACTTATACCG TGCCACAGGG TTGTCCAAGG CGACTTGGAC
5461 GTTGGAGGGT ATGAAGGGGG CCTCGCTGTG AAAGAGTGGC TTCTCGCGCA CGAAGGTCAT
5521 CGGCTCGGTA AGCCAGGGCT CGGGGAAATC TCAGGAAGTG GAGATGGCGC CGGATTGATC
5581 AATGGACGGG CAGATgctag cATGAAAATG GAAGAGCTGT TCAAGAAACA CAAGATCGTT
5641 GCCGTGCTGC GTGCCAATAG TGTGGAAGAA GCGAAAAAGA AAGCGCTGGC GGTTTTCCTG
5701 GGCGGCGTTC ATCTGATTGA AATTACCTTT ACCGTGCCGG ATGCGGATAC CGTGATTAAG
5761 GAACTGAGCT TTCTGAAGGA AATGGGCGCG ATTATTGGTG CGGGCACCGT GACCAGCGTG
5821 GAGCAGTGCC GTAAAGCGGT GGAAAGTGGC GCCGAATTCA TTGTGAGTCC GCACCTGGAC
5881 GAGGAAATTA GCCAATTTTG CAAGGAGAAG GGTGTGTTCT ATATGCCAGG CGTTATGACC
5941 CCGACCGAAC TGGTGAAAGC CATGAAACTG GGCCATACCA TCTTAAAACT GTTTCCGGGT
6001 GAGGTGGTGG GTCCGCAGTT TGTTAAAGCG ATGAAAGGTC CGTTTCCGAA TGTGAAATTT
6061 GTGCCAACCG GCGGTGTTAA TCTGGACAAT GTGTGCGAAT GGTTCAAAGC GGGCGTGCTG
6121 GCCGTGGGCG TGGGCAGCGC GTTAGTGAAA GGCACCCCGG TGGAAGTGGC GGAAAAGGCC
6181 AAGGCGTTCG TTGAGAAGAT TCGTGGCTGC ACCGAAcata tgggcagcgg cgccacaaac
6241 ttctctctgc taaagcaagc aggtgatgtt gaagaaaacc ccgggcctAC TAGTGCTGGA
6301 AAGCTTggct ccggcgaggg caggggaagt cttctaacat gcggggacgt ggaggaaaat
6361 cccggcccaG gtaccggtgg cggaagtgga ggtggttcag gttctatgga caaagactgc
6421 gaaatgaagc gcaccaccct ggatagccct ctgggcaagc tggaactgtc tgggtgcgaa
6481 cagggcctgc accgtatcat cttcctgggc aaaggaacat ctgccgccga cgccgtggaa
6541 gtgcctgccc cagccgccgt gctgggcgga ccagagccac tgatgcaggc caccgcctgg
6601 ctcaacgcct actttcacca gcctgaggcc atcgaggagt tccctgtgcc agccctgcac
6661 cacccagtgt tccagcagga gagctttacc cgccaggtgc tgtggaaact gctgaaagtg
6721 gtgaagttcg gagaggtcat cagctacagc cacctggccg ccctggccgg caatcccgcc
6781 gccaccgccg ccgtgaaaac cgccctgagc ggaaatcccg tgcccattct gatcccctgc
6841 caccgggtgg tgcagggcga cctggacgtg gggggctacg agggcgggct cgccgtgaaa
6901 gagtggctgc tggcccacga gggccacaga ctgggcaagc ctgggctggg tGGATCCACC
6961 GGATCTAGAT AACTGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA
7021 AAAAACCTCC CACACCTCCC CCTGAACCTG AACGCGGCCG CtggGTCGAC AATCAACCTC
7081 TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC
7141 TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCGTT AACTAAACTT GTTTATTGCA
7201 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT
7261 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGAAT
7321 TGACTCAAAT GATGTCAATT AGTCTATCAG AAGCTCATCT GGTCTCCCTT CCGGGGGACA
7381 AGACATCCCT GTTTAATATT TAAACAGCAG TGTTCCCAAA CTGGGTTCTT ATATCCCTTG
7441 CTCTGGTCAA CCAGGTTGCA GGGTTTCCTG TCCTCACAGG AACGAAGTCC CTAAAGAAAC
7501 AGTGGCAGCC AGGTTTAGCC CCGGAATTGA CTGGATTCCT TTTTTAGGGC CCATTGGTAT
7561 GGCTTTTTCC CCGTATCCCC CCAGGTGTCT GCAGGCTCAA AGAGCAGCGA GAAGCGTTCA
7621 GAGGAAAGCG ATCCCGTGCC ACCTTCCCCG TGCCCGGGCT GTCCCCGCAC GCTGCCGGCT
7681 CGGGGATGCG GGGGGAGCGC CGGACCGGAG CGGAGCCCCG GGCGGCTCGC TGCTGCCCCC
7741 TAGCGGGGGA GGGACGTAAT TACATCCCTG GGGGCTTTGG GGGGGGGCTG TCCCTGATAT
7801 CTATAACAAG AAATATATAT ATAATAAGTT ATCACGTAAG TAGAACATGA AATAACAATA
7861 TAATTATCGT ATGAGTTAAT CTTAAAGTCA CGTAAAAGAT AATCATGCGT CATTTTGACT
7921 CACGCGGTCG TTATAGTTCA AAATCAGTGA CACTTACCGC ATTGACAAGC ACGCCTCACG
7981 GGAGCTCCAA GCGGCGACTG AGATGTCCTA AATGCACAGC GACGGATTCG CGCTATTTAG
8041 AAAGAGAGAG CAATATTTCA AGAATGCATG CGTCAATTTT ACGCAGACTA TCTTTCTAGG
8101 GTTAATCTAG CTGCATCAGG ATCATATCGT CGGGTCTTTT TTCCGGCTCA GTCATCGCCC
8161 AAGCTGGCGC TATCTGGGCA TCGGGGAGGA AGAAGCCCGT GCCTTTTCCC GCGAGGTTGA
8221 AGCGGCATGG AAAGAGTTTG CCGAGGATGA CTGCTGCTGC ATTGACGTTG AGCGAAAACG
8281 CACGTTTACC ATGATGATTC GGGAAGGTGT GGCCATGCAC GCCTTTAACG GTGAACTGTT
8341 CGTTCAGGCC ACCTGGGATA CCAGTTCGTC GCGGCTTTTC CGGACACAGT TCCGGATGGT
8401 CAGCCCGAAG CGCATCAGCA ACCCGAACAA TACCGGCGAC AGCCGGAACT GCCGTGCCGG
8461 TGTGCAGATT AATGACAGCG GTGCGGCGCT GGGATATTAC GTCAGCGAGG ACGGGTATCC
8521 TGGCTGGATG CCGCAGAAAT GGACATGGAT ACCCCGTGAG TTACCCGGCG GGCGCGCTTG
8581 GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC
8641 AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC
8701 ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG
8761 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT
8821 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC
8881 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA
8941 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT
9001 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC
9061 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT
9121 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG
9181 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG
9241 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT
9301 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG
9361 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC
9421 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA
9481 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
9541 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
9601 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
9661 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT
9721 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
9781 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA
9841 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAG
//