https://gitlab.com/tgwgraham/papa_paper_plasmids
Tip revision: 984803582c0023a9fe975ec6d41be3d723c2737b authored by Thomas Graham on 08 July 2022, 01:06:31 UTC
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Tip revision: 9848035
ptg742-ef1alpha-fkbp-snapf-3xnls.gb
LOCUS pTG742_EF1alpha-FKBP-SN 7956 bp ds-DNA circular 17-NOV-2020
DEFINITION .
KEYWORDS "creator:Gina Dailey - Darzacq/Tjian Lab" "marker:AmpR, PuroR"
FEATURES Location/Qualifiers
primer complement(108..126)
/label="oTG1552_pBS_diffusion_substrate_300bp"
/note="sequence: ctctagcttcccggcaaca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS complement(650..718)
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter complement(719..823)
/label="AmpR promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(802..823)
/label="oTG1179_FRT_AmpR_5’"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCcgcggaacccctatttgtttat"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(821..839)
/label="oTG1553_pBS_diffusion_substrate_1kb"
/note="sequence: ttttcggggaaatgtgcgc"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
rep_origin complement(849..1303)
/label="f1 ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 1441..1460
/label="oTG1336_M13F_extended"
/note="sequence: GTTGTAAAACGACGGCCAGT"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind 1443..1460
/label="M13-fwd"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 1444..1460
/label="M13 fwd"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
misc_feature 1590..1602
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 1637..1652
/label="IR (inverted repeat) truncated?"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
promoter 2071..2570
/label="PGK promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS 2592..3191
/label="PuroR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
polyA_signal 3224..3448
/label="bGH poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter 3488..3699
/label="EF-1α core promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
LTR 3712..3980
/label="5' LTR (truncated)"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 3991..4010
/label="oTG1576_3xFlag-Halo_upstream_F"
/note="sequence: CCGGCGCCTACTCTAGCCAC"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 3991..4034
/label="oTG1560_FKBP-SNAPf_F1"
/note="sequence: CCGGCGCCTACTCTAGCCACCATGggagtgcaggtggaaaccat"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 4006..4030
/label="oTG939_pcDNA_FKBP_F"
/note="sequence: CCACTAGTCCAGTGTGGTGGCCACCatgggagtgcaggtggaaa"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS 4012..4014
/label="ATG"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
misc_feature 4015..4034
/label="F primer"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
CDS 4015..4335
/label="FKBP"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 4015..4347
/label="Translation 4015-4347"
CDS 4015..5034
/label="Translation 4015-5034"
primer complement(4312..4336)
/label="oTG940_FKBP_60mer_R"
/note="sequence: TCTTGAACAGCTCTTCCATTTTttccagttttagaagctccacatc"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature complement(4328..4347)
/label="R primer"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer complement(4328..4370)
/label="oTG1561_FKBP-SNAPf_R1"
/note="sequence: TTCATTTCGCAGTCTTTGTCCATaccaccagcactttccagtt"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer 4347..4369
/label="oTG1577_Halo_linker_SNAPf_F"
/note="sequence: CGACGCTCGAGATTTCCGGCggtagtggtaccggttctATGGACAAAGACTGCGAAATGA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 4348..4370
/label="oTG1580_Kozak_SNAPf_F"
/note="sequence: CCGGCGCCTACTCTAGCCACCatggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 4348..4370
/label="oTG1562_FKBP-SNAPf_F2"
/note="sequence: atggacaaagactgcgaaatgaa"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
gene 4348..4893
/label="SNAP26f"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 4348..4893
/label="SNAPf"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
CDS 4348..4893
/label="Translation 4348-4893"
source 4348..4893
/label="source"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(4874..4893)
/label="oTG1581_SNAPf_3’_R"
/note="sequence: acccagcccaggcttgccca"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(4874..4894)
/label="oTG1578_SNAPf_noNLS_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTGGATCCacccagcccaggcttgccca"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(4874..4913)
/label="oTG1563_FKBP-SNAPf_R2"
/note="sequence: TCTCCGCTGCCGGAAATCTCacccagcccaggcttgccca"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
CDS 4894..4905
/label="HaloTag®"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 4909..4929
/label="oTG1415_7-SspB-p65-Fwd-OptoFly-GA"
/note="sequence: ggagatggcgctggactgatc"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
CDS 4909..4932
/label="GDGAGLIN linker"
/ApEinfo_revcolor=#cd4489
/ApEinfo_fwdcolor=#cd4489
misc_feature 4935..4935
/label="add_after"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 4945..4965
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 4969..4989
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
CDS 4993..5013
/label="SV40 NLS"
/ApEinfo_revcolor=#993366
/ApEinfo_fwdcolor=#993366
primer 5012..5036
/label="oTG967_pHalo-Stop_F"
/note="sequence: TAGGATCCACCGGATCTAGATAACT"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(5019..5047)
/label="oTG1579_3xNLS_downstream_R"
/note="sequence: TGATTATGATCAGTTATCTAGATCCGGTG"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 5028..5038
/label="stop codons"
/ApEinfo_revcolor=#ff0000
/ApEinfo_fwdcolor=#ff0000
misc_feature 5115..5115
/label="add_before"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
CDS 5115..5132
/label="MCS"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
polyA_signal 5248..5369
/label="SV40 poly(A) signal"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer complement(5341..5369)
/label="oTG885_SV40_pA_F"
/note="sequence: TAAGATACATTGATGAGTTTGGACAAACC"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature complement(6129..6147)
/label="IR (inverted repeat)"
/ApEinfo_revcolor=#339966
/ApEinfo_fwdcolor=#339966
misc_feature complement(6151..6163)
/label="PB Terminal repeat"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind complement(6654..6670)
/label="M13 rev"
/ApEinfo_revcolor=#a020f0
/ApEinfo_fwdcolor=#a020f0
protein_bind 6678..6694
/label="lac operator"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
promoter complement(6702..6708)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(6709..6726)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer complement(6720..6741)
/label="oTG980_Vector-Fwd-GA"
/note="sequence: ccggatgacagtgacgaggagaactgaACCCCAGGCTTTacacTTTATG"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
promoter complement(6727..6732)
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
protein_bind 6747..6768
/label="CAP binding site"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
primer 6799..6819
/label="oTG1156_mCherry-LacI-Rev"
/note="sequence: AAGCTTCCATTCGGGAAACCTGTCGtgCCAG"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer 6819..6839
/label="oTG1089_Rev-LacI-NLS-NOSTOP-GA"
/note="sequence: AACCTTCCTCTTCTTCTTAGGAGGCTGCATTAATGAATCGGCCA"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 6819..6840
/label="oTG1084_Rev-LacI-GA"
/note="sequence: TCAAACCTTCCTCTtcTTcTTaGgagGCTGCATTAATGAATCGGCCAA"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer 6876..6893
/label="oTG775_pET_BbvCI_insert_seq"
/note="sequence: TTCCGCTTCCTCGCTCAC"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
rep_origin complement(7056..7643)
/label="ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer 7697..7720
/label="oTG1180_AmpR_seq"
/note="sequence: ACGTTAAGGGATTTTGGTCATGAG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(7783..7813)
/label="oTG1177_FRT_AmpR_3’flanking"
/note="sequence: CTATACTTTCTAGAGAATAGGAACTTCctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer complement(7783..7813)
/label="oTG1554_pBS_diffusion_substrate_3kb"
/note="sequence: ctgtcagaccaagtttactcatatatacttt"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer 7784..7813
/label="oTG1182_E75_AmpR_F"
/note="sequence: gactaactgccactcgcagcgcgcagatcgAAGTATATATGAGTAAACTTGGTCTGACAG"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer 7813..7837
/label="oTG1549_pBS_849_adapter"
/note="sequence: GCTGTGTCTTATTACACACAGTCGttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
CDS complement(join(7814..7956,1..649))
/label="AmpR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 7814..7837
/label="oTG1176_FRT_AmpR_3’"
/note="sequence: CTATTCTCTAGAAAGTATAGGAACTTCttaccaatgcttaatcagtgaggc"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(7860..7882)
/label="oTG1550_pBS_diffusion_substrate_100bp"
/note="sequence: caggcaactatggatgaacgaaa"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 ACCCACGCTC ACCGGTTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC
61 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG
121 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA
181 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA
241 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA
301 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA
361 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA
421 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG
481 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG
541 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG
601 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG
661 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC
721 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
781 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
841 TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT
901 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA
961 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
1021 GGACTCCAAC GTCAAAGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA
1081 TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA
1141 GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG
1201 AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA
1261 ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCATTCAGG
1321 CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGGCG
1381 AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTCC CAGTCACGAC
1441 GTTGTAAAAC GACGGCCAGT GAGCGCGCCT CGTTCATTCA CGTTTTTGAA CCCGTGGAGG
1501 ACGGGCAGAC TCGCGGTGCA AATGTGTTTT ACAGCGTGAT GGAGCAGATG AAGATGCTCG
1561 ACACGCTGCA GAACACGCAG CTAGATTAAC CCTAGAAAGA TAATCATATT GTGACGTACG
1621 TTAAAGATAA TCATGTGTAA AATTGACGCA TGTGTTTTAT CGGTCTGTAT ATCGAGGTTT
1681 ATTTATTAAT TTGAATAGAT ATTAAGTTTT ATTATATTTA CACTTACATA CTAATAATAA
1741 ATTCAACAAA CAATTTATTT ATGTTTATTT ATTTATTAAA AAAAACAAAA ACTCAAAATT
1801 TCTTCTATAA AGTAACAAAA CTTTTATGAG GGACAGCCCC CCCCCAAAGC CCCCAGGGAT
1861 GTAATTACGT CCCTCCCCCG CTAGGGGGCA GCAGCGAGCC GCCCGGGGCT CCGCTCCGGT
1921 CCGGCGCTCC CCCCGCATCC CCGAGCCGGC AGCGTGCGGG GACAGCCCGG GCACGGGGAA
1981 GGTGGCACGG GATCGCTTTC CTCTGAACGC TTCTCGCTGC TCTTTGAGCC TGCAGACACC
2041 TGGGGGGATA CGGGGAAAAG GCGAACGCGT gggtagggga ggcgcttttc ccaaggcagt
2101 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
2161 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
2221 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
2281 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
2341 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
2401 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
2461 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
2521 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc
2581 caAGCTTCAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC
2641 CCAGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG
2701 TCGATCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG
2761 TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA
2821 CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG
2881 AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC
2941 GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA
3001 AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC
3061 CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA
3121 CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC
3181 CCGGTGCCTG AGCGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
3241 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC
3301 ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT
3361 ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG
3421 CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AACGCGATCG CTCCGGTGCC
3481 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC
3541 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC
3601 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG
3661 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC
3721 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC
3781 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA
3841 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC
3901 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT
3961 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CTCTAGCCAC CATGggagtg
4021 caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc
4081 gtggtgcact acaccgggat gcttgaagat ggaaagaaat ttgattcctc ccgggacaga
4141 aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg
4201 gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat
4261 ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt cgatgtggag
4321 cttctaaaac tggaaagtgc tggtggtatg gacaaagact gcgaaatgaa gcgcaccacc
4381 ctggatagcc ctctgggcaa gctggaactg tctgggtgcg aacagggcct gcaccgtatc
4441 atcttcctgg gcaaaggaac atctgccgcc gacgccgtgg aagtgcctgc cccagccgcc
4501 gtgctgggcg gaccagagcc actgatgcag gccaccgcct ggctcaacgc ctactttcac
4561 cagcctgagg ccatcgagga gttccctgtg ccagccctgc accacccagt gttccagcag
4621 gagagcttta cccgccaggt gctgtggaaa ctgctgaaag tggtgaagtt cggagaggtc
4681 atcagctaca gccacctggc cgccctggcc ggcaatcccg ccgccaccgc cgccgtgaaa
4741 accgccctga gcggaaatcc cgtgcccatt ctgatcccct gccaccgggt ggtgcagggc
4801 gacctggacg tggggggcta cgagggcggg ctcgccgtga aagagtggct gctggcccac
4861 gagggccaca gactgggcaa gcctgggctg ggtGAGATTT CCGGCagcgg agatggcgct
4921 ggactgatca acggcCGAGC TGATCCAAAA AAGAAGAGAA AGGTAGATCC AAAAAAGAAG
4981 AGAAAGGTAG ATCCAAAAAA GAAGAGAAAG GTAGGATCCA CCGGATCTAG ATAACTGATC
5041 ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC
5101 CCCCTGAACC TGAACGCGGC CGCtggGTCG ACAATCAACC TCTGGATTAC AAAATTTGTG
5161 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT
5221 TAATGCCTTT GTATCATGCG TTAACTAAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA
5281 TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT
5341 GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGGA ATTGACTCAA ATGATGTCAA
5401 TTAGTCTATC AGAAGCTCAT CTGGTCTCCC TTCCGGGGGA CAAGACATCC CTGTTTAATA
5461 TTTAAACAGC AGTGTTCCCA AACTGGGTTC TTATATCCCT TGCTCTGGTC AACCAGGTTG
5521 CAGGGTTTCC TGTCCTCACA GGAACGAAGT CCCTAAAGAA ACAGTGGCAG CCAGGTTTAG
5581 CCCCGGAATT GACTGGATTC CTTTTTTAGG GCCCATTGGT ATGGCTTTTT CCCCGTATCC
5641 CCCCAGGTGT CTGCAGGCTC AAAGAGCAGC GAGAAGCGTT CAGAGGAAAG CGATCCCGTG
5701 CCACCTTCCC CGTGCCCGGG CTGTCCCCGC ACGCTGCCGG CTCGGGGATG CGGGGGGAGC
5761 GCCGGACCGG AGCGGAGCCC CGGGCGGCTC GCTGCTGCCC CCTAGCGGGG GAGGGACGTA
5821 ATTACATCCC TGGGGGCTTT GGGGGGGGGC TGTCCCTGAT ATCTATAACA AGAAATATAT
5881 ATATAATAAG TTATCACGTA AGTAGAACAT GAAATAACAA TATAATTATC GTATGAGTTA
5941 ATCTTAAAGT CACGTAAAAG ATAATCATGC GTCATTTTGA CTCACGCGGT CGTTATAGTT
6001 CAAAATCAGT GACACTTACC GCATTGACAA GCACGCCTCA CGGGAGCTCC AAGCGGCGAC
6061 TGAGATGTCC TAAATGCACA GCGACGGATT CGCGCTATTT AGAAAGAGAG AGCAATATTT
6121 CAAGAATGCA TGCGTCAATT TTACGCAGAC TATCTTTCTA GGGTTAATCT AGCTGCATCA
6181 GGATCATATC GTCGGGTCTT TTTTCCGGCT CAGTCATCGC CCAAGCTGGC GCTATCTGGG
6241 CATCGGGGAG GAAGAAGCCC GTGCCTTTTC CCGCGAGGTT GAAGCGGCAT GGAAAGAGTT
6301 TGCCGAGGAT GACTGCTGCT GCATTGACGT TGAGCGAAAA CGCACGTTTA CCATGATGAT
6361 TCGGGAAGGT GTGGCCATGC ACGCCTTTAA CGGTGAACTG TTCGTTCAGG CCACCTGGGA
6421 TACCAGTTCG TCGCGGCTTT TCCGGACACA GTTCCGGATG GTCAGCCCGA AGCGCATCAG
6481 CAACCCGAAC AATACCGGCG ACAGCCGGAA CTGCCGTGCC GGTGTGCAGA TTAATGACAG
6541 CGGTGCGGCG CTGGGATATT ACGTCAGCGA GGACGGGTAT CCTGGCTGGA TGCCGCAGAA
6601 ATGGACATGG ATACCCCGTG AGTTACCCGG CGGGCGCGCT TGGCGTAATC ATGGTCATAG
6661 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC
6721 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC
6781 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA
6841 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG
6901 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG
6961 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG
7021 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
7081 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
7141 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
7201 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC
7261 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
7321 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
7381 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
7441 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA
7501 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAGAGTT GGTAGCTCTT
7561 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA
7621 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC
7681 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA
7741 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA
7801 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT
7861 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT
7921 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAG
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